scholarly journals Direct somatic embryogenesis and plant regeneration in tea by temporary liquid immersion Embriogenesis somatik langsung dan regenerasi tanaman teh melalui perendaman sesaat

2016 ◽  
Vol 68 (1) ◽  
Author(s):  
J S TAHARDI ◽  
Tatik RAISAWATI ◽  
Imron RIYADI ◽  
W A DODD
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Culture Technique ◽  
Direct Somatic Embryogenesis ◽  
Temporary Immersion System ◽  
Single Leaf ◽  
Half Strength ◽  
Rapid Dissemination ◽  
Planting Materials

Ringkasan Perbanyakan tanaman teh [Camellia sinen­sis (L.) O. Kuntze] melalui stek tunas berdaun tunggal hanya dapat menghasilkan klon unggul dalam jumlah terbatas. Oleh sebab itu diperlukan metode alternatif dengan teknik kultur sel dan jaringan untuk perbanyakan klonal secara cepat. Dalam penelitian ini dikembangkan metode yang lebih efektif untuk regenerasi tanaman teh melalui embriogenesis somatik langsung. Massa pro­embriogenik dari eksplan kotiledon dihasilkan dengan frekuensi 56,7% dalam media MS padat setengah konsentrasi yang mengandung BAP 2 mg1L. Proliferasi, perkembangan, pendewasaan dan perkecambahan embrio somatik diperoleh dengan sistem perendaman sesaat (SPS) yang menggunakan media MS cair setengah konsen­trasi, yang diperkaya dengan zat pengatur tumbuh dengan berbagai konsentrasi. Proliferasi embrio meningkat 4,3 kali dalam media yang diberi BAP 2 mglL; perkembangan dan pendewasaannya meningkat dengan penambahan kinetin dan ABA masing-masing pada konsentrasi 0,1 mg1L yang 30% diantaranya berkecambah dan membentuk planlet tanpa penambahan zat pengatur tumbuh. Protokol SPS tersebut merupakan sistem in vitro yang berpotensi bagi proliferasi dan perkembang­an embrio somatik tanaman teh yang cepat dan sinkron dari kultur kotiledon, serta regenerasinya menjadi planlet tanpa melalui fase kalus.Summary Tea propagation by single-leaf bud cuttings has limited applications for rapid dissemination of planting materials from new elite clones. An alternative method for rapid cloning by cell and tissue culture technique is necessary. In this study we have established an improved method for tea [Camellia sinensis (L.) O. Kuntze] plant regenera­tion via direct somatic embryogenesis. Clumps of proembryogenic masses were initiated at a fre­quency of 56.7% from cotyledonary slices cul­tured on a half-strength MS agar-gelled medium supplemented with 2 mg/L BAP. Proliferation, development, maturation and germination of so­matic embryos were achieved using the temporary immersion system (TIS) provided with half­strength MS liquid media supplemented with varying concentrations of growth regulators. Em­bryo proliferation increased by 4.3-fold in me­dium provided with 2 mg/L BAP; their develop­ment and maturation were enhanced by the presence of both kinetin and ABA at 0.1 mg/L each. Germination and plant recovery were achieved at a frequency of about 30% without the use of growth regulators. The TIS protocol des­cribed above represents an in vitro system poten­tial for rapid proliferation and synchronized development of tea somatic embryos from cotyledon cultures, and their regeneration into plantlets without an intervening callus phase.

scholarly journals Direct somatic embryogenesis and plant regeneration in tea by temporary liquid immersion Embriogenesis somatik langsung dan regenerasi tanaman teh melalui perendaman sesaat

2016 ◽  
Vol 68 (1) ◽  
Author(s):  
J S TAHARDI ◽  
Tatik RAISAWATI ◽  
Imron RIYADI ◽  
W A DODD
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Culture Technique ◽  
Direct Somatic Embryogenesis ◽  
Temporary Immersion System ◽  
Single Leaf ◽  
Half Strength ◽  
Rapid Dissemination ◽  
Planting Materials

Ringkasan Perbanyakan tanaman teh [Camellia sinen­sis (L.) O. Kuntze] melalui stek tunas berdaun tunggal hanya dapat menghasilkan klon unggul dalam jumlah terbatas. Oleh sebab itu diperlukan metode alternatif dengan teknik kultur sel dan jaringan untuk perbanyakan klonal secara cepat. Dalam penelitian ini dikembangkan metode yang lebih efektif untuk regenerasi tanaman teh melalui embriogenesis somatik langsung. Massa pro­embriogenik dari eksplan kotiledon dihasilkan dengan frekuensi 56,7% dalam media MS padat setengah konsentrasi yang mengandung BAP 2 mg1L. Proliferasi, perkembangan, pendewasaan dan perkecambahan embrio somatik diperoleh dengan sistem perendaman sesaat (SPS) yang menggunakan media MS cair setengah konsen­trasi, yang diperkaya dengan zat pengatur tumbuh dengan berbagai konsentrasi. Proliferasi embrio meningkat 4,3 kali dalam media yang diberi BAP 2 mglL; perkembangan dan pendewasaannya meningkat dengan penambahan kinetin dan ABA masing-masing pada konsentrasi 0,1 mg1L yang 30% diantaranya berkecambah dan membentuk planlet tanpa penambahan zat pengatur tumbuh. Protokol SPS tersebut merupakan sistem in vitro yang berpotensi bagi proliferasi dan perkembang­an embrio somatik tanaman teh yang cepat dan sinkron dari kultur kotiledon, serta regenerasinya menjadi planlet tanpa melalui fase kalus.Summary Tea propagation by single-leaf bud cuttings has limited applications for rapid dissemination of planting materials from new elite clones. An alternative method for rapid cloning by cell and tissue culture technique is necessary. In this study we have established an improved method for tea [Camellia sinensis (L.) O. Kuntze] plant regenera­tion via direct somatic embryogenesis. Clumps of proembryogenic masses were initiated at a fre­quency of 56.7% from cotyledonary slices cul­tured on a half-strength MS agar-gelled medium supplemented with 2 mg/L BAP. Proliferation, development, maturation and germination of so­matic embryos were achieved using the temporary immersion system (TIS) provided with half­strength MS liquid media supplemented with varying concentrations of growth regulators. Em­bryo proliferation increased by 4.3-fold in me­dium provided with 2 mg/L BAP; their develop­ment and maturation were enhanced by the presence of both kinetin and ABA at 0.1 mg/L each. Germination and plant recovery were achieved at a frequency of about 30% without the use of growth regulators. The TIS protocol des­cribed above represents an in vitro system poten­tial for rapid proliferation and synchronized development of tea somatic embryos from cotyledon cultures, and their regeneration into plantlets without an intervening callus phase.

Somatic Embryogenesis and In vitro Plant Regeneration in Vigna trilobata (L.) Verd. - A Potential Pasture Legume

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Long Term Study ◽  
Continuous Application ◽  
First Time ◽  
Pasture Legume

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

scholarly journals The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

2011 ◽  
Vol 27 (2) ◽  
Author(s):  
Rina Arimarsetiowati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Callus Formation ◽  
Culture Technique ◽  
Plant Production ◽  
Direct Somatic Embryogenesis ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

scholarly journals MICROPROPAGATION OF PHALAENOPSIS SPP. BY SOMATIC EMBRYOGENESIS TECHNIQUE

2018 ◽  
Vol 6 ◽  
pp. 1185-1191
Author(s):  
Minh Van Tran
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Cell ◽  
Embryonic Cell ◽  
Cell Suspensions ◽  
Ms Medium ◽  
Callus Initiation ◽  
Set Up ◽  
Planting Materials ◽  
In Vitro Embryogenesis

Phalaenopsis spp. was regularly produced through micropropagation by protocorm like bodies (PLBs); micropropagation takes a lot of labor, and has high cost of seedlings, energy and material. The purpose of this paper was to study the new technique of using in vitro embryogenesis culturing for microprogation. The method involved using protocorm like bodies as planting materials. PLBs were cut into slices and placed on the medium for callus initiation. The callus was initiated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l) or 2,4D (1 mg/l) and was proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l). Somatic cell suspensions were initiated and proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (0.5, 1 mg/l). Somatic cell suspensions were differentiated to embryonic cell suspensions on the MS medium supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Embryonic cell suspensions were plated and regenerated on the medium: 1/2MS supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Micropropagation of Phalaenopsis sp. via the embryogenesis technique was set up to produce 5,800 plantlets per one liter of somatic embryogenesis suspension.

scholarly journals Peculiarities of in vitro rooting of some species of Carlina L. genus

1970 ◽  
pp. 215-220
Author(s):  
N. B. Kravets ◽  
M. Z. Mosula ◽  
N. M. Drobyk ◽  
N. V. Tulaidan ◽  
M. B. Cheterbok
Keyword(s):  
Acid Solution ◽  
Growth Regulators ◽  
Root Formation ◽  
Cultivated Plants ◽  
Treat Ment ◽  
Presowing Treatment ◽  
Murashige Skoog ◽  
Half Strength ◽  
Semi Solid

Aim. The aim of the study was to choose conditions for rooting improvement of in vitro cultivated plants of some species of Carlina L. genus. Methods. For receiving and rooting of aseptic sprouts, seeds of Carlina acaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl were subjected to presowing treatment with gibberellic acid solution (GA3) or indolebyturic acid solution (IBA). Sterilized seeds were planted in sterile Petri dishes on semi-solid Murashige, Skoog nutrient medium with half-strength concentrations of macro- and microsalts without growth regulators. Results. It was found that with the seed soaking of C. acaulis, C. cirsioides and C. onopordifolia in GA3 solution the percentage of root formation amounted to 33.3 %, 33.3 % and 22.2 % respectively. Presowing treat-ment of carlina seeds in IBA solution with concentration of 1000 mg for 2–4 hours before sterilization gave a positive effect: the percentage of root formation for C. acaulis, C. cirsioides and C. onorordifolia was 2.4–4.5 times higher compared to the treatment with GA3 solution. Conclusions. To form the root system of carlina plants it is effective to soak the seeds in the solution of IBA. Thus we were able to increase the percentage of rooting of C. sirsioides and C. onorordifolia plants to 100 %, C. acaulis plants – up to 80 % and avoid sprouts’ injury and changes in the concentra-tions of the IBA, which may occur during sterilization at high temperatures by using non-sterile solution of growth regulators. Keywords: Carlina àcaulis L., Carlina cirsioides Klok, Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl, in vitro, sprouts rooting.

scholarly journals In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1

2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Marlúcia Souza Pádua ◽  
Raíssa Silveira Santos ◽  
Luciano Vilela Paiva ◽  
Vanessa Cristina Stein ◽  
Luciano Coutinho Silva
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Oil Palm ◽  
Somatic Embryos ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Germination Percentage ◽  
Shoot Length ◽  
Indole Butyric Acid

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germinated from somatic embryos is a difficult step, yet it is of great importance for later acclimatization and success in propagation. The aim of this study was to evaluate the effect of the auxins indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of somatic embryos of Tenera hybrid oil palm. Plants obtained by somatic embryogenesis were inoculated in modified MS medium with 10% sucrose and 0.6% agar and supplemented with IAA or IBA at concentrations of 5 µM, 10 µM, and 15 µM, and the absence of growth regulators. After 120 days, the presence of roots, root type, length of the longest root, number of roots, number of leaves, and shoot length were analyzed. Growth regulators were favorable to rooting; plants cultivated with IBA growth regulator at 15 µM showed higher rooting percentage (87%) and better results for the parameters of number of roots (1.33) and shoot length (9.83).

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