Antifungal activity of methanol extract of Pluchea Lanceolata against some fungi

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
PREM SHANKAR PANDEY ◽  
UDAY PRATAP SINGH
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Pluchea Lanceolata

scholarly journals Antimicrobial and antioxidant potentials of non-cytotoxic extracts of corticolous lichens sampled in Armenia

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Razmik Sargsyan ◽  
Arsen Gasparyan ◽  
Gohar Tadevosyan ◽  
Hovik Panosyan
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cytotoxic Activities ◽  
Bacterial Strains ◽  
Evernia Prunastri ◽  
Wide Range ◽  
Antibacterial And Antifungal ◽  
Acetone Extracts

AbstractDue to wide range of secondary metabolites, lichens were used from antiquity as sources of colorants, perfumes and medicaments. This research focuses on exploring the antioxidant, antimicrobial and cytotoxic activities of methanol, ethanol, acetone extracts and aqueous infusions of corticolous lichens sampled from Armenia. Methanol, ethanol and acetone extracts from all tested lichens were active against Gram-positive bacterial strains. The most effective solvent to retrieve antimicrobial compounds was methanol. Aqueous infusions of tested lichens didn’t show any significant antibacterial and antifungal activity. The highest antimicrobial activity was observed for methanol extract of Ramalina sinensis. The minimum inhibitory concentration of methanol extract of Ramalina sinensis were 0.9–1.8 mg mL− 1. Pseudevernia furfuracea demonstrated antifungal activity (Ø 12 mm). Methanol extract of Parmelia sulcata demonstrated largest 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging activity (71 %). The cytotoxicity was measured on human HeLa (cervical carcinoma) cell lines using microculture tetrazolium test assay. The IC50 values estimated for methanol extracts of Peltigera praetextata, Evernia prunastri, Ramalina sinensis and Ramalina farinacea species in HeLa cell line were within 1.8–2.8 mg mL− 1 and considered as non-cytotoxic. Obtained results suggest that studied lichens can be prospective in biotechnologies as alternative sources of antimicrobial and antioxidant substances.

scholarly journals POTENSI EKSTRAK DAUN CENDANA (Santalum album L.) SEBAGAI SENYAWA PENGHAMBAT JAMUR Candida albicans

Jurnal Kimia ◽  
2019 ◽  
pp. 159
Author(s):  
K. Swandiyasa ◽  
N. M. Puspawati ◽  
I. A. R. A. Asih
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Santalum Album ◽  
Web Database ◽  
Nail Diseases ◽  
Leaf Powder ◽  
Santalum Album L ◽  
Crude Methanol Extract

Diseases caused by C. albicans (C. albicans) such as mouth, skin and nail  diseases are still commonly found in Indonesia. This study aimed to examine antifungal activity of n-hexane, chloroform, and n-butanol extracts of Santalum album (S.album) leaves in inhibiting the growth of C. albicans and to determine the minimum inhibitory concentration of the most active extract as well as to identify their active compounds. Antifungal activity testing was carried out using disc diffusion method and identification of active compound was performed using LC-MS /MS. Extraction of 1 kg sandalwood leaf powder with methanol yielded 86.80 gram of crude methanol extract. Partiiton 40 gram of the crude methanol extract with n-hexane, chloroform, and n-buthanol gave 12.60, 6.20 and 1.20 g of extracts respectively. The antifungal activity test results revealed that n-hexane extract was the most active in inhibiting the growth of C.albicans with inhibitory diameter of 13 mm as compared to chloroform 9 mm and n-buthanol 8 mm. The active n-hexane extract which is active as an antifungal is then made various variations to obtain a minimum inhibitory. The n-hexane extract, at concentrations of 95, 90 and 85% inhibited the growth of C.albican with inhibitory diameter of 12.04, 15.8, and 13 mm respectively. While at the concentarion of 80 % showed minimum inhibitory diameter of 9.32 mm. Based on analysis mass spectra of two peaks of LC-MS / MS chromatogram with MassLynx V4.1 programe and Chemspider web database suggested the presence of benzofurazan and picolylamine compounds which may contribute to the antifungal activityof n-hexane extract of S.album. Keywords: Sandalwood (Santalum album L.), antifungal, C. albicans, n-Hexane, LC-MS/MS

Additional Studies on the Antifungal Activity of a Methanol Extract of Ipomoea Carnea Subsp. Fistulosa and Octadecyl P-Coumarates

2016 ◽  
Vol 6 (3) ◽  
pp. 148-151 ◽  
Author(s):  
Eugene Sebastian John Nidiry ◽  
Girija Ganeshan ◽  
Ankanahalli Narayanashetty Lokesha ◽  
Nanjundagowda Ramachandran
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Ipomoea Carnea

scholarly journals Anti –phytopathogenic Activities of Cladophora glomerata extract against plant fungi

2014 ◽  
Vol 11 (2) ◽  
pp. 883-887 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antifungal Activity ◽  
Rhizoctonia Solani ◽  
Antimicrobial Agents ◽  
Methanol Extract ◽  
Cladophora Glomerata ◽  
Damping Off ◽  
Natural Antimicrobial ◽  
Plant Fungi ◽  
Pharmaceutical Industries ◽  
Crude Methanol Extract

The antiphytopathogenic effects of the crude methanol extract of Cladophora glomerata (Lin.) Kützing (Cladophoraceae) which isolated from Al Rashidiya region at north of Baghdad was investigated against two type of plant fungi (Pythium altimum and Rhizoctonia solani) which causes damping off disease where isolated from covered cucumber field in Al-Alyosifia region. Hot methanol extract showed antifungal activity against the two species of fungi in different concentrations(10,25,50 mg/ml) of extract to Cladophora glomerata as percentage inhibition (51.63 ,72.8 ,83.71) and (56.18 ,77.41 ,100)comparing with Pythium altimum and Rhizoctonia solani respectively .primary detection of active compounds showed that macroalgae (Cladophora glomerata) containing flavonoids,alkaloids, phenols and tannins. Our findings suggest the possibility of using the Cladophora glomerata as a novel source of natural antimicrobial agents for pharmaceutical industries.

scholarly journals Antimicrobial and Antioxidant Potentials of Non-cytotoxic Extracts of Corticolous Lichens Sampled in Armenia

2021 ◽  
Author(s):  
Razmik Sargsyan ◽  
Arsen Gasparyan ◽  
Gohar Tadevosyan ◽  
Hovik Panosyan
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cytotoxic Activities ◽  
Evernia Prunastri ◽  
Wide Range ◽  
Alternative Sources ◽  
Antibacterial And Antifungal ◽  
Acetone Extracts

Abstract Due to wide range of secondary metabolites, lichens were used from antiquity as sources of colorants, perfumes and medicaments. This research focuses on exploring the antioxidant, antimicrobial and cytotoxic activities of methanol, ethanol, acetone extracts and aqueous infusions of corticolous lichens sampled from Armenia. Methanol, ethanol and acetone extracts from all tested lichens were active against Gram-positive bacterial strains. The most effective solvent to retrieve antimicrobial compounds was methanol. Aqueous infusions of tested lichens didn’t show any significant antibacterial and antifungal activity. The highest antimicrobial activity was observed for methanol extract of Ramalina sinensis. The minimum inhibitory concentration of methanol extract of Ramalina sinensis were 0.9 to 1.8 mg mL-1. Pseudevernia furfuracea demonstrated antifungal activity (Ø 12 mm). Methanol extract of Parmelia sulcata demonstrated largest DPPH radical scavenging activity (71%). The cytotoxicity was measured on human HeLa (cervical carcinoma) cell lines using microculture tetrazolium test assay. The IC50 values estimated for methanol extracts of Peltigera praetextata, Evernia prunastri, R. sinensis and R. farinacea species in HeLa cell line were within 1.8-2.8 mg mL-1 and considered as non-cytotoxic. Obtained results suggest that studied lichens can be prospective in biotechnologies as alternative sources of antimicrobial and antioxidant substances.

scholarly journals The Effects of Tormentic Acid and Extracts from Callistemon citrinus on Candida albicans and Candida tropicalis Growth and Inhibition of Ergosterol Biosynthesis in Candida albicans

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Chido Bvumbi ◽  
Godloves Fru Chi ◽  
Marc Y. Stevens ◽  
Molly Mombeshora ◽  
Stanley Mukanganyama
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Candida Tropicalis ◽  
Methanol Extract ◽  
Fungal Infections ◽  
Water Extract ◽  
Antifungal Drugs ◽  
Ergosterol Content ◽  
Broth Microdilution Method ◽  
Tormentic Acid

Candida albicans and Candida tropicalis are the leading causes of human fungal infections worldwide. There is an increase in resistance of Candida pathogens to existing antifungal drugs leading to a need to find new sources of antifungal agents. Tormentic acid has been isolated from different plants including Callistemon citrinus and has been found to possess antimicrobial properties, including antifungal activity. The study aimed to determine the effects of tormentic and extracts from C. citrinus on C. albicans and C. tropicalis and a possible mode of action. The extracts and tormentic acid were screened for antifungal activity using the broth microdilution method. The growth of both species was inhibited by the extracts, and C. albicans was more susceptible to the extract compared to C. tropicalis. The growth of C. albicans was inhibited by 80% at 100 μg/ml of both the DCM: methanol extract and the ethanol: water extract. Tormentic acid reduced the growth of C. albicans by 72% at 100 μg/ml. The effects of the extracts and tormentic acid on ergosterol content in C. albicans were determined using a UV/Vis scanning spectrophotometer. At concentrations of tormentic acid of 25 μg/ml, 50 μg/ml, 100 μg/ml, and 200 μg/ml, the content of ergosterol was decreased by 22%, 36%, 48%, and 78%, respectively. Similarly, the DCM: methanol extract at 100 μg/ml and 200 μg/ml decreased the content by 78% and 88%, respectively. A dose-dependent decrease in ergosterol content was observed in cells exposed to miconazole with a 25 μg/ml concentration causing a 100% decrease in ergosterol content. Therefore, tormentic acid inhibits the synthesis of ergosterol in C. albicans. Modifications of the structure of tormentic acid to increase its antifungal potency may be explored in further studies.

scholarly journals Antifungal Activity of Senna alata, Senna bicapsularis and Pityrogramma calomelanos

2020 ◽  
pp. 11-21
Author(s):  
Florencia Guerra ◽  
Abdullah Adil Ansari ◽  
Rajini Kurup ◽  
Gomathinayagam Subramanian
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Plant Diseases ◽  
Diffusion Method ◽  
Curvularia Lunata ◽  
Diffusion Technique ◽  
Phytochemical Extraction ◽  
Pityrogramma Calomelanos ◽  
Senna Alata

Aims: Senna alata (Carrion crow bush), Senna bicapsularis (Money bush) and Pityrogramma calomelanos (Wild maran) are known for their value in traditional medicine. The study was an effort to identify, environment friendly antifungal compounds from these locally available plants against fungal pathogen Curvularia lunata. Study Design: An experiment based study done to identify antifungal property and phytochemical identification of C. lunata. Place and Duration of Study: Plants were collected from the coastal areas of Guyana and identified at the Biodiversity Center, University of Guyana, Guyana between January 2017- May 2017. Methodology: Phytochemical extraction was conducted using soxhlet and rotovap apparatus. Hexane, methanol and aqueous extracts of plants were analyzed for antifungal properties. Antifungal activity was tested using the poisoned food and well diffusion technique. Data analysis of the study was done using R-Studio Program for statistical computing and graphics. A Tukey test was done along with ANOVA and Boxplots. Results: Methanol extract of P. calomelanos showed maximum antifungal activity with an inhibition percentage of 60.3% at 400 µl, with poison food technique. With well diffusion method, P. calomelanos showed an inhibition zone of 54 mm at 500 µl. Saponins, steroids and glycosides were identified with methanol extract of P. calomelanos. Conclusion: The finding of this study indicates, the possible use of P. calomelanos extract as an antifungal agent against C. lunata and its potential to control fungal plant diseases.

scholarly journals ANTIFUNGAL POTENTIAL OF EXTRACTS AND FRACTIONS OF Randia nitida LEAVES ON SOYBEAN PATHOGENS AND THEIR PHYTOCHEMISTRY

2016 ◽  
Vol 29 (3) ◽  
pp. 594-602 ◽  
Author(s):  
STHEFANY CAROLINE BEZERRA DA CRUZ-SILVA ◽  
ROSEMARY MATIAS ◽  
JOSÉ ANTONIO MAIOR BONO ◽  
KAREN SILVA SANTOS ◽  
JULIANE LUDWIG
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Plant Pathogens ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Phytochemical Analysis ◽  
Total Phenols ◽  
Major Chemical ◽  
Crude Methanol Extract

ABSTRACT This study aimed to evaluate the antifungal activity of methanol extract and fractions of leaves of Randia nitida (Kunth) DC on Colletotrichum truncatum (Schw.) Andrus & Moore, Rhizoctonia solani Kühn and Sclerotinia sclerotiorum (Lib.) De Bary and perform chemical and antioxidant activity tests. Crude methanol extract (EBM) and fractions (hexane = FHX, dichloromethane = FDM and ethyl acetate = FAE) underwent classical phytochemical analysis and determination of total phenols, flavonoids and antioxidant activity. Samples were individually incorporated into PDA culture mediums (potato-dextrose-agar) at concentrations of 10, 20, 40, 80 and 160 µg mL-1 of extract or fraction. Mycelial growth was evaluated when control reached the edge of the plate. Phytochemical results indicated that flavonoids, steroids and triterpenoids are the major chemical constituents in both EBM and fractions. Total phenols, flavonoids and antioxidant activity were higher for FAE and EBM, followed by FDM and FHX; however, the latter showed higher content of steroids and triterpenoids compared to the other fractions. For C. truncatum, R. solani and S. sclerotiorum, the most efficient fractions were respectively FAE, FDM and FHX, all of them in the highest concentration (160 µg mL-1). The highest inhibition rate was observed against S. sclerotiorum, reaching 93.75%. We concluded that all fractions have antifungal activity on the plant pathogens, being FHX the most active against S. sclerotiorum, which might have been due to the presence of steroids and triterpenes.

scholarly journals Triterpenoid saponins from the roots of Acanthophyllum gypsophiloides Regel

2012 ◽  
Vol 8 ◽  
pp. 763-775 ◽  
Author(s):  
Elena A Khatuntseva ◽  
Vladimir M Men’shov ◽  
Alexander S Shashkov ◽  
Yury E Tsvetkov ◽  
Rodion N Stepanenko ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antifungal Activity ◽  
Methanol Extract ◽  
Keyhole Limpet Hemocyanin ◽  
2D Nmr ◽  
2D Nmr Spectroscopy ◽  
Triterpenoid Saponins ◽  
Pure Compounds ◽  
Ester Linkage ◽  
Quillaic Acid

Two new triterpenoid saponins 1 and 2 were isolated from the methanol extract of the roots of Acanthophyllum gypsophiloides Regel. These saponins have quillaic acid or gypsogenin moieties as an aglycon, and both bear similar sets of two oligosaccharide chains, which are 3-O-linked to the triterpenoid part trisaccharide α-L-Arap-(1→3)-[α-D-Galp-(1→2)]-β-D-GlcpA and pentasaccharide β-D-Xylp-(1→3)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-[β-D-Quip-(1→4)]-β-D-Fucp connected through an ester linkage to C-28. The structures of the obtained saponins were elucidated by a combination of mass spectrometry and 2D NMR spectroscopy. A study of acute toxicity, hemolytic, anti-inflammatory, immunoadjuvant and antifungal activity was carried out. Both saponins 1 and 2 were shown to exhibit immunoadjuvant properties within the vaccine composition with keyhole limpet hemocyanin-based immunogen. The availability of saponins 1 and 2 as individual pure compounds from the extract of the roots of A. gypsophiloides makes it a prospective source of immunoactive agents.

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