scholarly journals POTENSI EKSTRAK DAUN CENDANA (Santalum album L.) SEBAGAI SENYAWA PENGHAMBAT JAMUR Candida albicans

Jurnal Kimia ◽  
2019 ◽  
pp. 159
Author(s):  
K. Swandiyasa ◽  
N. M. Puspawati ◽  
I. A. R. A. Asih
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Santalum Album ◽  
Web Database ◽  
Nail Diseases ◽  
Leaf Powder ◽  
Santalum Album L ◽  
Crude Methanol Extract

Diseases caused by C. albicans (C. albicans) such as mouth, skin and nail  diseases are still commonly found in Indonesia. This study aimed to examine antifungal activity of n-hexane, chloroform, and n-butanol extracts of Santalum album (S.album) leaves in inhibiting the growth of C. albicans and to determine the minimum inhibitory concentration of the most active extract as well as to identify their active compounds. Antifungal activity testing was carried out using disc diffusion method and identification of active compound was performed using LC-MS /MS. Extraction of 1 kg sandalwood leaf powder with methanol yielded 86.80 gram of crude methanol extract. Partiiton 40 gram of the crude methanol extract with n-hexane, chloroform, and n-buthanol gave 12.60, 6.20 and 1.20 g of extracts respectively. The antifungal activity test results revealed that n-hexane extract was the most active in inhibiting the growth of C.albicans with inhibitory diameter of 13 mm as compared to chloroform 9 mm and n-buthanol 8 mm. The active n-hexane extract which is active as an antifungal is then made various variations to obtain a minimum inhibitory. The n-hexane extract, at concentrations of 95, 90 and 85% inhibited the growth of C.albican with inhibitory diameter of 12.04, 15.8, and 13 mm respectively. While at the concentarion of 80 % showed minimum inhibitory diameter of 9.32 mm. Based on analysis mass spectra of two peaks of LC-MS / MS chromatogram with MassLynx V4.1 programe and Chemspider web database suggested the presence of benzofurazan and picolylamine compounds which may contribute to the antifungal activityof n-hexane extract of S.album. Keywords: Sandalwood (Santalum album L.), antifungal, C. albicans, n-Hexane, LC-MS/MS

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

scholarly journals SENYAWA ANTIMAKAN TERHADAP Epilachna Sparsa DARI BATANG GAYAM (Inocarpus fagiferus Fosb.)

Jurnal Kimia ◽  
2020 ◽  
pp. 51
Author(s):  
S. R. Santi ◽  
I M. Sukadana ◽  
N. N. T. Hanayanthi
Keyword(s):  
Ethyl Acetate ◽  
Mass Spectra ◽  
Methanol Extract ◽  
Leaf Tissue ◽  
Hexane Extract ◽  
Antifeedant Activity ◽  
Insecticide Activity ◽  
Hexane Extracts ◽  
Bottom Side ◽  
Crude Methanol Extract

Epilachna sparsa is one of the plant pests that damage the epidermal layer on the bottom side of the leaves, so it can lead to damage of leaf tissue and only the skeleton remains. Gayam (Inocarpus fagiferus) is one of the species of the Legumineceae family which has insecticide activity. This study aims to isolate and identify the antifeedant triterpenoid compound from stem of gayam (Inocarpus fagiferus Fosb.) against Epilachna sparsa larvae. Dried powder of 1200 g gayam stems produced 17.82 g of crude methanol extract with antifeedant activity of 60.74% at a concentration of 5% (w/v) which was further separated by partition to obtain n-hexane, ethyl acetate, and water extracts. Extracts of n-hexane, ethyl acetate, and water at 5% (w/v) concentration showed antifeedant activity of 46.74%, 18.09%, and 8.31% respectively. Futhermore, the most active n-hexane extract was identified by phytochemical tests and LC-MS/MS. The phytochemical test showed n-hexane extract contains triterpenoid compounds. The mass spectra from LC-MS/MS chromatogram peaks with MassLynx V4.1 software and Chemspider database for n-hexane extracts which were previously purified by SPE (Solid method) Phase Extraction) using methanol and dichloromethane (DCM) eluents show the presence of components of flavonol pyrogallol, sucrose2,3,3 ', 4', 6-pentaacetate, soyasaponin I, and a compound with a molecular weight of 685,225 g/mol which cannot be identified further because there is no match spectra with the database.    Keywords: antifeedant, Epilachna sparsa, gayam, Inocarpus fagiferus Fosb., triterpenoid.

scholarly journals Anti –phytopathogenic Activities of Cladophora glomerata extract against plant fungi

2014 ◽  
Vol 11 (2) ◽  
pp. 883-887 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antifungal Activity ◽  
Rhizoctonia Solani ◽  
Antimicrobial Agents ◽  
Methanol Extract ◽  
Cladophora Glomerata ◽  
Damping Off ◽  
Natural Antimicrobial ◽  
Plant Fungi ◽  
Pharmaceutical Industries ◽  
Crude Methanol Extract

The antiphytopathogenic effects of the crude methanol extract of Cladophora glomerata (Lin.) Kützing (Cladophoraceae) which isolated from Al Rashidiya region at north of Baghdad was investigated against two type of plant fungi (Pythium altimum and Rhizoctonia solani) which causes damping off disease where isolated from covered cucumber field in Al-Alyosifia region. Hot methanol extract showed antifungal activity against the two species of fungi in different concentrations(10,25,50 mg/ml) of extract to Cladophora glomerata as percentage inhibition (51.63 ,72.8 ,83.71) and (56.18 ,77.41 ,100)comparing with Pythium altimum and Rhizoctonia solani respectively .primary detection of active compounds showed that macroalgae (Cladophora glomerata) containing flavonoids,alkaloids, phenols and tannins. Our findings suggest the possibility of using the Cladophora glomerata as a novel source of natural antimicrobial agents for pharmaceutical industries.

scholarly journals Antifungal Activity of Senna alata, Senna bicapsularis and Pityrogramma calomelanos

2020 ◽  
pp. 11-21
Author(s):  
Florencia Guerra ◽  
Abdullah Adil Ansari ◽  
Rajini Kurup ◽  
Gomathinayagam Subramanian
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Plant Diseases ◽  
Diffusion Method ◽  
Curvularia Lunata ◽  
Diffusion Technique ◽  
Phytochemical Extraction ◽  
Pityrogramma Calomelanos ◽  
Senna Alata

Aims: Senna alata (Carrion crow bush), Senna bicapsularis (Money bush) and Pityrogramma calomelanos (Wild maran) are known for their value in traditional medicine. The study was an effort to identify, environment friendly antifungal compounds from these locally available plants against fungal pathogen Curvularia lunata. Study Design: An experiment based study done to identify antifungal property and phytochemical identification of C. lunata. Place and Duration of Study: Plants were collected from the coastal areas of Guyana and identified at the Biodiversity Center, University of Guyana, Guyana between January 2017- May 2017. Methodology: Phytochemical extraction was conducted using soxhlet and rotovap apparatus. Hexane, methanol and aqueous extracts of plants were analyzed for antifungal properties. Antifungal activity was tested using the poisoned food and well diffusion technique. Data analysis of the study was done using R-Studio Program for statistical computing and graphics. A Tukey test was done along with ANOVA and Boxplots. Results: Methanol extract of P. calomelanos showed maximum antifungal activity with an inhibition percentage of 60.3% at 400 µl, with poison food technique. With well diffusion method, P. calomelanos showed an inhibition zone of 54 mm at 500 µl. Saponins, steroids and glycosides were identified with methanol extract of P. calomelanos. Conclusion: The finding of this study indicates, the possible use of P. calomelanos extract as an antifungal agent against C. lunata and its potential to control fungal plant diseases.

Antibacterial Activity of Nanoparticles Produced by Ethanol Extract of Boesenbergia rotunda Rhizome Loaded with Chitosan and Alginic Acid Against Streptococcus mutans by In Vitro

2017 ◽  
Vol 7 (03) ◽  
Author(s):  
Sri Atun ◽  
Sri Handayani ◽  
Melia Aliffiana ◽  
Hajar Nur Afifah ◽  
Anna Rakhmawati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
Alginic Acid ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

scholarly journals Evaluation of Antimicrobial Activities of Crude Methanol Extract of Phoenix dactylifera Seeds on Clinical Isolates of Different Strains of E. coli

2019 ◽  
pp. 1-7
Author(s):  
C. Eze Chinelo ◽  
N. Ezeibe Ezinwanne ◽  
A. Ozioko Chizoba ◽  
C. Agbo Martina ◽  
N. Ugwu Chineye ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Phoenix Dactylifera ◽  
Antimicrobial Activities ◽  
Seed Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

Aim: This study was conducted to evaluate the antimicrobial activities of seed extract of Phoenix dactylifera. Study Design: Extraction of active ingredients of Phoenix dactylifera seeds using methanol and its effects on selected clinical isolates and isolated strains of E. coli. Place and Duration of Study: `Department of Phatrmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical, University of Nigeria, Nsukka between march 2017 and October 2018. Methodology: The seed of phoenix dactylifera were washed thoroughly to get rid of any adhering date flesh, air dried and ground. The ground powders were subjected to extraction by cold maceration using methanol. Preliminary sensitivity test was carried out against eight microorganisms, namely: Pseudomonas aeruginosa, E. coli, Enterococcus, Klebsiella, Staph. spp, Salmonella spp, Bacillus sp and Candida albicans using agar diffusion method. Antimicrobial susceptibility tests were carried out on isolated strains of E. coli from urine samples using agar diffusion method. The minimal inhibitory concentrations of crude methanol extract of seeds of Phoenix dactylifera were determined using agar diffusion method. The phytochemical analysis was conducted to determine the secondary metabolites. Results: The preliminary sensitivity test performed shows that the crude methanol extract of date seed were sensitive against all organisms tested. The active ingredients of the extract showed activities against all strains of E. coli tested though their zones of inhibition vary (18-21mm). The minimum inhibitory concentration (MIC) value ranges from 10-19.9 mg/ml. The phytochemical analysis of crude methanol seed extract of P. dacterifera indicated the presence of alkaloids, flavonoids, tannins, carbohydrates, proteins, reducing sugar, sterols and Terpenes, Anthraquinone glycosides. Conclusion: The present study provides the scientific information about Phoenix dactylifera seed activity.

scholarly journals ANTIFUNGAL POTENTIAL OF EXTRACTS AND FRACTIONS OF Randia nitida LEAVES ON SOYBEAN PATHOGENS AND THEIR PHYTOCHEMISTRY

2016 ◽  
Vol 29 (3) ◽  
pp. 594-602 ◽  
Author(s):  
STHEFANY CAROLINE BEZERRA DA CRUZ-SILVA ◽  
ROSEMARY MATIAS ◽  
JOSÉ ANTONIO MAIOR BONO ◽  
KAREN SILVA SANTOS ◽  
JULIANE LUDWIG
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Plant Pathogens ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Phytochemical Analysis ◽  
Total Phenols ◽  
Major Chemical ◽  
Crude Methanol Extract

ABSTRACT This study aimed to evaluate the antifungal activity of methanol extract and fractions of leaves of Randia nitida (Kunth) DC on Colletotrichum truncatum (Schw.) Andrus & Moore, Rhizoctonia solani Kühn and Sclerotinia sclerotiorum (Lib.) De Bary and perform chemical and antioxidant activity tests. Crude methanol extract (EBM) and fractions (hexane = FHX, dichloromethane = FDM and ethyl acetate = FAE) underwent classical phytochemical analysis and determination of total phenols, flavonoids and antioxidant activity. Samples were individually incorporated into PDA culture mediums (potato-dextrose-agar) at concentrations of 10, 20, 40, 80 and 160 µg mL-1 of extract or fraction. Mycelial growth was evaluated when control reached the edge of the plate. Phytochemical results indicated that flavonoids, steroids and triterpenoids are the major chemical constituents in both EBM and fractions. Total phenols, flavonoids and antioxidant activity were higher for FAE and EBM, followed by FDM and FHX; however, the latter showed higher content of steroids and triterpenoids compared to the other fractions. For C. truncatum, R. solani and S. sclerotiorum, the most efficient fractions were respectively FAE, FDM and FHX, all of them in the highest concentration (160 µg mL-1). The highest inhibition rate was observed against S. sclerotiorum, reaching 93.75%. We concluded that all fractions have antifungal activity on the plant pathogens, being FHX the most active against S. sclerotiorum, which might have been due to the presence of steroids and triterpenes.

scholarly journals CRUDE EXTRACTS OF MAGNOLIA ALBA FLOWERS USING N-HEXANE AND METHANOL SOLVENTS AND THEIR ANTI-ACNE POTENTIAL

2021 ◽  
Vol 10 (6) ◽  
pp. 3789-3793
Author(s):  
Sofia Munira
Keyword(s):  
Propionibacterium Acnes ◽  
Methanol Extract ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Positive Control ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Bacterial Inhibition

This study aimed to test the antibacterial activity of n-hexane and methanol extracts of white cempaka flowers (Magnolia alba) against Propionibacterium acnes bacteria. The extract was obtained by using the maceration method for 72 hours. Each extract was tested for its antibacterial properties using the disc diffusion method with the variant concentration of the extract used was 0.05%; 2%; 4%; 6%; and 8%. The results showed that the methanol extract had a higher inhibition zone than the n-hexane extract. In n-hexane extract, the lowest concentration of 0.05% showed an inhibition zone of 6.6 mm and the highest concentration of 8% had an inhibition zone of 9.6 mm. Whereas in methanol extract, the inhibition zone obtained was 7.3 mm at a low concentration of 0.05%, but at the highest concentration of 8% the inhibition zone obtained was 11.6 mm. The effect of extract concentration on the diameter of the inhibition zone formed is very significant (directly proportional), where the higher the concentration used, the larger the diameter of the inhibition zone produced. Antibacterial testing for both extracts showed a weak (< 10 mm) to moderate (> 10 mm) bacterial inhibition, which was similar with the inhibition zone obtained from the positive control (11-11.3 mm; moderate bacterial inhibition).

scholarly journals Screening of Antimicrobial Activity of Various Extracts of the Stem Justicia gendarussa

2019 ◽  
pp. 1-7
Author(s):  
Dhanapal Venkatachalam ◽  
Akhib Rahman ◽  
Basil Sunny ◽  
Jensy Jacob ◽  
Nikhil Kuriyan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Aqueous Extract ◽  
Antimicrobial Agents ◽  
Pathogenic Fungus ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Standard Drug ◽  
Antibacterial And Antifungal

Objective: The present study was designed to check in-vitro efficacy of Antibacterial and antifungal activity of aqueous and hexane extracts of stem of Justicia gendarussa against selected bacterial and fungal strains. Justicia gendarussa, belonging to the family Acanthaceae, commonly known as ‘‘vatham kolli’ was used in treatment of bronchitis, inflammation, eye diseases, ear ache, vaginal discharges, rheumatism, dysentery, eczema and jaundice. Methodology: Hexane and aqueous extracts of Justicia gendarussa was used for antimicrobial screening. Antibacterial activity was tested against pathogenic bacterial strains Eschericha coli and Staphylococcus aureus. Also antifungal activity was tested against one human pathogenic fungal strain i.e. Candida albicans. Antibacterial and antifungal activities of Justicia gendarussa extract was carried out by using disc diffusion method. Results: Aqueous extract of Justicia gendarussa showed the largest zone of inhibition (5mm) against Eschericha coli, at 50µgm/ml and 3mmzone of inhibition against S. aures, at 50µgm/ml. Hexane extract does not   showing activity against to human pathogenic E. coli and S. aures, at 50µgm/ml. Aqueous extract has shown more potent antibacterial activity against Eschericha coli. Aqueous extract was found to be more effective against human pathogenic fungus Candida albicuns than hexane extract and standard drug. The zone of inhibition of Aqueous extract was 11mm and the zone of inhibition of hexane extract was7mm and standard only 2mm.The phytochemical screening demonstrated the presence of different types of compounds like terpenoids, tannins, and flavonoids which may contribute for the anti-microbial action of this plant. Conclusions: These findings provide scientific evidence of traditional use of Justicia gendarussa and also indicate the potential of this plant for the development of antimicrobial agents.

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