scholarly journals ANTIFUNGAL POTENTIAL OF EXTRACTS AND FRACTIONS OF Randia nitida LEAVES ON SOYBEAN PATHOGENS AND THEIR PHYTOCHEMISTRY

2016 ◽  
Vol 29 (3) ◽  
pp. 594-602 ◽  
Author(s):  
STHEFANY CAROLINE BEZERRA DA CRUZ-SILVA ◽  
ROSEMARY MATIAS ◽  
JOSÉ ANTONIO MAIOR BONO ◽  
KAREN SILVA SANTOS ◽  
JULIANE LUDWIG
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Plant Pathogens ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Phytochemical Analysis ◽  
Total Phenols ◽  
Major Chemical ◽  
Crude Methanol Extract

ABSTRACT This study aimed to evaluate the antifungal activity of methanol extract and fractions of leaves of Randia nitida (Kunth) DC on Colletotrichum truncatum (Schw.) Andrus & Moore, Rhizoctonia solani Kühn and Sclerotinia sclerotiorum (Lib.) De Bary and perform chemical and antioxidant activity tests. Crude methanol extract (EBM) and fractions (hexane = FHX, dichloromethane = FDM and ethyl acetate = FAE) underwent classical phytochemical analysis and determination of total phenols, flavonoids and antioxidant activity. Samples were individually incorporated into PDA culture mediums (potato-dextrose-agar) at concentrations of 10, 20, 40, 80 and 160 µg mL-1 of extract or fraction. Mycelial growth was evaluated when control reached the edge of the plate. Phytochemical results indicated that flavonoids, steroids and triterpenoids are the major chemical constituents in both EBM and fractions. Total phenols, flavonoids and antioxidant activity were higher for FAE and EBM, followed by FDM and FHX; however, the latter showed higher content of steroids and triterpenoids compared to the other fractions. For C. truncatum, R. solani and S. sclerotiorum, the most efficient fractions were respectively FAE, FDM and FHX, all of them in the highest concentration (160 µg mL-1). The highest inhibition rate was observed against S. sclerotiorum, reaching 93.75%. We concluded that all fractions have antifungal activity on the plant pathogens, being FHX the most active against S. sclerotiorum, which might have been due to the presence of steroids and triterpenes.

scholarly journals Seasonal effects and antifungal activity from bark chemical constituents of Sterculia apetala (Malvaceae) at Pantanal of Miranda, Mato Grosso do Sul, Brazil

2015 ◽  
Vol 45 (3) ◽  
pp. 283-292 ◽  
Author(s):  
Fernanda Mussi FONTOURA ◽  
Rosemary MATIAS ◽  
Juliane LUDWIG ◽  
Ademir Kleber Morbeck de OLIVEIRA ◽  
José Antonio Maior BONO ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Chemical Constituents ◽  
Chemical Study ◽  
Stem Bark ◽  
Seasonal Effects ◽  
Chemical Components ◽  
Phytochemical Analysis ◽  
Wet Season ◽  
Mycelium Growth ◽  
Mato Grosso

In the Southern Pantanal, the hyacinth macaw (Anodorhynchus hyacinthinus), an endangered species, often chooses the manduvi tree (Sterculia apetala) as a nesting site, because of its physical properties. In addition, the chemical composition of the wood may also contribute to a nesting selection by the hyacinth macaws. The objective of this study was to determine the main chemical components of S. apetala bark for two seasons, and evaluate its fungicidal potential. Bark samples from S. apetala trees with and without nests of A. hyacinthinus were collected in January (wet season) and August (dry season) of 2012. The inhibition of mycelium growth (MGI) from tree samples with and without nests were assessed using a phytochemical analysis to evaluate their antifungal activity against Trichoderma sp. Phytochemical analysis confirmed the presence of phenolic compounds and flavonoids. In both seasons, samples obtained from nested trees had higher content of total phenols than those collected from non-nested trees. The average content of total flavonoids was higher in January for samples with nest and in August for samples without nest. All selected samples showed antifungal activity, and those with nest collected in August (peak of hyacinth macaw breeding) resulted in an MGI of 51.3%. Therefore, this percentage, related to the content of flavonoids and the presence of coumarins, may influence the reproductive success of hyacinth macaws and other species of birds, in this region. This is the first chemical study report with the stem bark of S. apetala.

scholarly journals Chemical Constituents and Antioxidant Activity of Ficus callosa

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Phan Van Kiem ◽  
Nguyen Xuan Cuong ◽  
Nguyen Xuan Nhiem ◽  
Dan Thi Thuy Hang ◽  
Nguyen Hoai Nam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Chemical Evidence

One new megastigmane glycoside, ficalloside (1), and eleven known compounds, were isolated from methanol extract of Ficus callosa leaves by repeated column chromatography. Their structures were established on the basis of spectral and chemical evidence. The antioxidant activities of these compounds were measured using the oxygen radical absorbance capacity (ORAC) assay. Compound 8 exhibited potent antioxidant activity of 10.6 μM trolox equivalents at the concentration of 2 μM. At this concentration, compounds 4-7 and 9-12 showed significant antioxidant activity with ranging of 2.1~6.1 μM trolox equivalents.

scholarly journals DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF PRASMAN LEAF EXTRACTS (AYAPANA TRIPLINERVIS [VAHL]) AND THE TOTAL FLAVONOID AND PHENOL CONTENTS OF THE MOST ACTIVE EXTRACTS

2020 ◽  
pp. 107-111
Author(s):  
YUNITRIANTI ◽  
BERNA ELYA ◽  
ARIKADIA NOVIANI
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Total Flavonoid ◽  
Dpph Assay ◽  
Frap Assay ◽  
Methanol Extracts ◽  
Uv Visible ◽  
Antioxidant Effects

Objective: Prasman (Ayapana triplinervis [Vahl]) is a plant that can eliminate free radicals through its antioxidant effects. However, little research hasbeen conducted to explore the antioxidant activity of the plant.Methods: A. triplinervis (Vahl) leaves, which were determined by the Indonesian Institute of Sciences (LIPI) Bogor, were used in this study. Meanwhile,extraction was performed using n-hexane, ethyl acetate, and methanol as the solvents. Based on the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay usinga UV–visible spectrophotometer and based on ferric reducing antioxidant power (FRAP) assay using a microplate reader.Results: Based on the DPPH assay using a UV–visible spectrophotometer, n-hexane, ethyl acetate, and methanol extracts with a final concentrationof 25 μg/mL inhibited DPPH radical production by 38.91, 51.03, and 54.06%, respectively. Using the percent inhibition, the IC50 for the ethyl acetateand methanol extracts were 28.71 and 23.472 μg/mL, respectively. Based on FRAP assay using a microplate reader, the n-hexane, ethyl acetate, andmethanol extracts had ferrous equivalent antioxidant capacity values of 460, 828.99, and 940.22 μmol/g, respectively. The methanol extract had thegreatest antioxidant activity. The ethyl acetate and methanol extracts at the initial concentrations contained total phenol levels of 12.06 and 42.11 mgGAE/g extract, respectively, as well as total flavonoid levels of 3.24 and 3.41 mg QE/g extract, respectively.Conclusion: Based on the determination of phenol and flavonoid levels, the methanol extract had the greatest antioxidant effects.

scholarly journals POTENSI EKSTRAK DAUN CENDANA (Santalum album L.) SEBAGAI SENYAWA PENGHAMBAT JAMUR Candida albicans

Jurnal Kimia ◽  
2019 ◽  
pp. 159
Author(s):  
K. Swandiyasa ◽  
N. M. Puspawati ◽  
I. A. R. A. Asih
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Santalum Album ◽  
Web Database ◽  
Nail Diseases ◽  
Leaf Powder ◽  
Santalum Album L ◽  
Crude Methanol Extract

Diseases caused by C. albicans (C. albicans) such as mouth, skin and nail  diseases are still commonly found in Indonesia. This study aimed to examine antifungal activity of n-hexane, chloroform, and n-butanol extracts of Santalum album (S.album) leaves in inhibiting the growth of C. albicans and to determine the minimum inhibitory concentration of the most active extract as well as to identify their active compounds. Antifungal activity testing was carried out using disc diffusion method and identification of active compound was performed using LC-MS /MS. Extraction of 1 kg sandalwood leaf powder with methanol yielded 86.80 gram of crude methanol extract. Partiiton 40 gram of the crude methanol extract with n-hexane, chloroform, and n-buthanol gave 12.60, 6.20 and 1.20 g of extracts respectively. The antifungal activity test results revealed that n-hexane extract was the most active in inhibiting the growth of C.albicans with inhibitory diameter of 13 mm as compared to chloroform 9 mm and n-buthanol 8 mm. The active n-hexane extract which is active as an antifungal is then made various variations to obtain a minimum inhibitory. The n-hexane extract, at concentrations of 95, 90 and 85% inhibited the growth of C.albican with inhibitory diameter of 12.04, 15.8, and 13 mm respectively. While at the concentarion of 80 % showed minimum inhibitory diameter of 9.32 mm. Based on analysis mass spectra of two peaks of LC-MS / MS chromatogram with MassLynx V4.1 programe and Chemspider web database suggested the presence of benzofurazan and picolylamine compounds which may contribute to the antifungal activityof n-hexane extract of S.album. Keywords: Sandalwood (Santalum album L.), antifungal, C. albicans, n-Hexane, LC-MS/MS

Phytochemical Analysis and GC-MS Profiling in the Flower of Plumeria alba

2020 ◽  
Vol 981 ◽  
pp. 280-284
Author(s):  
Siti Aisyah Mohamad ◽  
Noor Suhana Adzahar ◽  
Muhammad Nadeem Akhtar ◽  
Seema Zareen ◽  
Thong Chuan Lee
Keyword(s):  
Chemical Constituents ◽  
Volatile Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Therapeutic Properties ◽  
Phytochemical Constituents ◽  
Screening Assays ◽  
Major Chemical ◽  
Ethylhexyl Phthalate

Therapeutic properties of the medicinal plant are due to the presence of phytochemical constituents. The phytochemical constituents of Plumeria alba flower were investigated by phytochemical screening assays and gas chromatography-mass spectrometry (GC-MS). The phytochemical screening of hexane, dichloromethane, ethyl acetate, butanol, and aqueous extracts of P. alba flower showed it contains a various concentration of saponins, flavonoids, tannins, steroids, volatile oil and phenolic compounds. Several major chemical constituents that were identified is squalene, bis(2-ethylhexyl) phthalate, methyl (methyl 4-O-methyl-α-d-mannopyranoside) uronate and tricyclo [7.2.0.0(2,6)] undecan-5-ol, 2,6,10,10-tetramethyl- (isomer 2) by using GC-MS technique.

scholarly journals ANTIOXIDANT ACTIVITY, PHYTOCHEMICAL ANALYSIS AND TOTAL POLYPHENOLICS CONTENT OF ESSENTIAL OIL, METHANOL EXTRACT AND METHANOL FRACTIONS FROM COMMELINA NUDIFLORA

2018 ◽  
Vol 10 (8) ◽  
pp. 36 ◽  
Author(s):  
Muhammad Dawood Shah ◽  
Mohammad Iqbal
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Ethyl Acetate ◽  
Palmitic Acid ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Hexadecanoic Acid ◽  
Methanol Extracts

Objective: In the present study, the essential oil, methanol extract, and methanol fractions (n-hexane, chloroform, ethyl acetate, and n-butanol) obtained from Commelina nudiflora were investigated for the free radical scavenging effects and phytochemical analysis.Methods: The antioxidative effect of the essential oil, methanol extracts and methanol fractions were evaluated using 2, 2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Total phenolic and flavonoid contents were determined using Folin-Ciocalteau and aluminium chloride reagents respectively. The phytochemical analyses of the essential oil, methanol extracts and methanol fractions were performed by gas chromatography and mass spectrometry (GCMS). Results: The antioxidant, total phenolic and total flavonoid contents of butanol, ethyl acetate and chloroform fractions were higher followed by methanol extract, hexane fraction and essential oil. Phytochemical analysis indicated the presence of alkaloid, saponin, steroid, phytosterols, triterpenoids and tannins etc. The identified bioactive constituents of essential oil, methanol extract and methanol fractions of C. nudiflora were indole, 2-methoxy-4-vinylphenol, 2-pentadecanone, 6,10,14-trimethyl, phenol, benzyl alcohol, eugenol, phenol, 2, 4-bis (1,1-dimethylethyl), hexadecanoic acid, ethyl ester (palmitic acid ester), n-hexadecanoic acid (palmitic acid), 9, 12-octadecadienoic acid, (linoleic acid) and phytol. All identified bioactive compounds and their derivatives were generally reported with antimicrobial, antioxidant, anti-inflammatory and antitumor properties.Conclusion: The obtained data suggest that the essential oil, methanol extract and methanol fractions of C. nudiflora possess remarkable antioxidant activities and vital phytochemicals. Thus the plant can be a utilized as a potential source of nutraceutical with antioxidant activity.

scholarly journals Deoxypodophyllotoxin Content and Antioxidant Activity of Aerial Parts of Anthriscus sylvestris Hoffm.

2006 ◽  
Vol 61 (9-10) ◽  
pp. 658-662 ◽  
Author(s):  
Stefano Dall’Acqua ◽  
Michela Giorgetti ◽  
Rinaldo Cervellati ◽  
Gabbriella Innocenti
Keyword(s):  
Antioxidant Activity ◽  
Chlorogenic Acid ◽  
Crude Extract ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Aerial Parts ◽  
Anthriscus Sylvestris ◽  
Oscillating Reaction ◽  
Crude Methanol Extract ◽  
Different Altitudes

AbstractDeoxypodophyllotoxin content of the aerial parts of Anthriscus sylvestris Hoffm. growing at different altitudes was evaluated in comparison to the roots. The lignan accumulation in ground parts was at least double compared to aerial ones. In addition antioxidant-guided fractionation of the crude methanol extract of aerial parts was performed with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. Active fractions contained mainly luteolin-7-O-glucoside and chlorogenic acid. Antioxidant properties of both crude extract and isolated compounds were also investigated with the Briggs-Rauscher (BR) oscillating reaction. A satisfactory agreement between the results obtained with the two methods was observed.

Comparative Study on Two Variants of Laghupanchamula (A Compound Ayurvedic Formulation) for Important Groups of Phytochemicals and Antioxidant Activity

2017 ◽  
Vol 2 (3) ◽  
pp. 157-163
Author(s):  
Shivani Ghildiyal ◽  
Apurva Joshi ◽  
Vinod K Joshi
Keyword(s):  
Antioxidant Activity ◽  
Comparative Study ◽  
High Performance ◽  
Hplc Analysis ◽  
Shikimic Acid ◽  
Ricinus Communis ◽  
Chemical Constituents ◽  
Tribulus Terrestris

ABSTRACT Introduction Laghupanchamula is a compound formulation prepared by combinations of roots of five herbs. Two variants of Laghupanchamula have been described in Ayurvedic classics where beside four common herbs fifth one is either Gokshura (Tribulus terrestris L.) or Eranda (Ricinus communis L.). The objective of the study is to make comparison between two variants of Laghupanchmula with respect to important group of phytochemicals and antioxidant activity to corroborate the science behind their therapeutic utility. Materials and methods Standard methods have been followed for quantitative determination of total quantity of phenols, tannins, flavonoids, and flavonols, and in vitro antioxidant activity in variants of Laghupanchmula formulations. Qualitative high-performance liquid chromatography (HPLC) analysis has also been performed to establish presence/absence of important chemical constituents in formulations. Results Additional quantity of phenols, tannins, flavonoids, and flavonols has been observed in Laghupanchamula variant containing Eranda than the variant containing Gokshura. Greater antioxidant activity has also been found in formulation containing Eranda. The HPLC analysis revealed the presence of shikimic acid, gallic acid, catechin in both formulations, but rutin has been found only in formulation containing Eranda. Conclusion From the results of experiments it has been observed that the formulation containing Eranda has more antioxidant activity as it contains more quantities of phenol, tannins, flavonoids, and flavonols than the formulation containing Gokshura. How to cite this article Ghildiyal S, Joshi A, Joshi VK. Comparative Study on Two Variants of Laghupanchamula (A Compound Ayurvedic Formulation) for Important Groups of Phytochemicals and Antioxidant Activity. J Drug Res Ayurvedic Sci 2017;2(3):157-163.

scholarly journals Anti –phytopathogenic Activities of Cladophora glomerata extract against plant fungi

2014 ◽  
Vol 11 (2) ◽  
pp. 883-887 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antifungal Activity ◽  
Rhizoctonia Solani ◽  
Antimicrobial Agents ◽  
Methanol Extract ◽  
Cladophora Glomerata ◽  
Damping Off ◽  
Natural Antimicrobial ◽  
Plant Fungi ◽  
Pharmaceutical Industries ◽  
Crude Methanol Extract

The antiphytopathogenic effects of the crude methanol extract of Cladophora glomerata (Lin.) Kützing (Cladophoraceae) which isolated from Al Rashidiya region at north of Baghdad was investigated against two type of plant fungi (Pythium altimum and Rhizoctonia solani) which causes damping off disease where isolated from covered cucumber field in Al-Alyosifia region. Hot methanol extract showed antifungal activity against the two species of fungi in different concentrations(10,25,50 mg/ml) of extract to Cladophora glomerata as percentage inhibition (51.63 ,72.8 ,83.71) and (56.18 ,77.41 ,100)comparing with Pythium altimum and Rhizoctonia solani respectively .primary detection of active compounds showed that macroalgae (Cladophora glomerata) containing flavonoids,alkaloids, phenols and tannins. Our findings suggest the possibility of using the Cladophora glomerata as a novel source of natural antimicrobial agents for pharmaceutical industries.

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