scholarly journals NEW, GREEN AND MINIATURIZED ANALYTICAL METHOD FOR DETERMINATION OF CEFADROXIL MONOHYDRATE IN CAPSULES

2019 ◽  
Vol 3 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Bianca Marco ◽  
Ana Kogawa ◽  
Hérida Salgado
Keyword(s):  
Analytical Chemistry ◽  
Standard Sample ◽  
Low Cost ◽  
Visible Region ◽  
Hplc Method ◽  
Minimal Amount ◽  
Green Analytical Chemistry ◽  
Color Reagent ◽  
Routine Quality Control

Cefadroxil, an oral antimicrobial, presents few techniques optimized for the reduction of solvents and toxic residues and/or non-use of them. So, a quantitative, new and miniaturized method for determination of cefadroxil monohydrate in capsules has been developed and validated by spectrophotometric method in the visible region according to the international guidelines. The analyzes were performed using microplates containing 96 wells, 1 % of phenolphthalein and sodium hydroxide 0.1 M as reagent at 552 nm. The method was (i) linear in the range of 15-115 µg mL-1, (ii) selective when comparing standard, sample, adjuvants and color reagent, (iii) precise with deviations below 4 %, (iv) accurate when comparing the proposed method with the HPLC method, (v) robusts by making small and deliberate modifications to the method, (vi) besides being fast, low cost, eco-friendly and generates minimal amount of waste. The method can be applied to the routine quality control of cefadroxil monohydrate in capsules and an effective and accessible alternative that contemplates the concepts of current and sustainable green analytical chemistry.

A New Ecological HPLC Method for Determination of Vancomycin Dosage form

2020 ◽  
Vol 7 (2) ◽  
pp. 82-90
Author(s):  
Patricia Aleixa do Nascimento ◽  
Ana Carolina Kogawa ◽  
Hérida R.N. Salgado
Keyword(s):  
Analytical Chemistry ◽  
Quality Control ◽  
Acetic Acid ◽  
Dosage Form ◽  
Uv Light ◽  
Hplc Method ◽  
Green Analytical Chemistry ◽  
Vancomycin Dosage ◽  
Routine Quality Control

Aims: To develop and validate a new ecological HPLC method for the determination of vancomycin dosage form. Background: Vancomycin is an important antimicrobial. According to the literature, there are many methods that use HPLC, but none of these methods follow the green analytical chemistry principles. Objective: Therefore, a green analytical method to quantify vancomycin in lyophilized powder for injectable solution by HPLC was developed. Materials and Methods: It uses less quantity of toxic solvents, minimizing the costs and optimizing the time of analysis. Water + 0.1% acetic acid and ethanol (85:15, v/v), 0.5 mL min-1, and C18 column (15 cm) at 280 nm were used. Results and Discussion: The method was linear in the range of 40 to 140 μg mL-1, with a correlation coefficient of 0.9998. It was selective when subjected to acid 0.1M, basic 0.01M, oxidative 0.3%, UV light and neutral degradation in a bath of 60 ºC for 8 hours. The precision of the method was proved at intraday (RSD 1.08%), interday (RSD 0.47%) and intermediate levels (RSD 2.35%). It was accurate with a mean recovery of 100.19% and robust when changes were performed in seven parameters of the method and analyzed by the Youden and Steiner test. Conclusion: The method can be applied to routine quality control of vancomycin lyophilized powder for injectable solution as an ecological and sustainable alternative that contemplates the green analytical chemistry and the current pharmaceutical analyses.

scholarly journals GREEN ANALYTICAL METHOD FOR QUANTIFICATION OF SECNIDAZOLE IN TABLETS BY HPLC-UV

2018 ◽  
Vol 2 (2) ◽  
pp. 20-26
Author(s):  
Jéssica Lima ◽  
Ana Kogawa ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Analytical Chemistry ◽  
Particle Size ◽  
Analytical Method ◽  
Mobile Phase ◽  
Toxic Waste ◽  
Green Analytical Chemistry ◽  
Injection Volume ◽  
As Stress ◽  
Routine Quality Control

A simple, rapid, economic and green analytical method was validated for the determination of secnidazole in tablets. The aim was to contribute to the green analytical chemistry since it has low use of organic solvent and low production of toxic waste. For the HPLC-UV method, the mobile phase was a mixture of purified water + 0.7 % acetic acid and ethanol (78:22, v/v), flow rate was 1.3 mL min-1 on column CN Phenomenex Luna (250 x 4.60 mm, 5 μm particle size), injection volume was 20 μL with UV detection at 318 nm and retention time of 4.26 minutes. The method was linear over the concentration range of 5-100 μg mL-1 (r = 0.9998) with limits of detection and quantitation of 0.533 e 1.615 μg mL-1, respectively. The precision of the method showed RSD less than 2 %. The accuracy determined by the average recoveries was 99.58 %. The secnidazole tablets were subjected to oxidation, acid, alkaline, neutral and photolysis degradation as stress conditions and the method was considered as indicative of stability. The method is adequate and safe to be a great alternative method in routine quality control analyzes for determination and quantification of secnidazole tablets.

scholarly journals ECO-FRIENDLY GREEN LIQUID CHROMATOGRAPHIC FOR DETERMINATION OF DOXYCYCLINE IN TABLETS AND IN THE PRESENCE OF ITS DEGRADATION PRODUCTS

2018 ◽  
Vol 2 (2) ◽  
pp. 49-55
Author(s):  
Loren Ghidini ◽  
Ana Kogawa ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Analytical Chemistry ◽  
Quality Control ◽  
Degradation Products ◽  
Toxic Waste ◽  
Green Analytical Chemistry ◽  
Photolytic Degradation ◽  
Liquid Chromatographic ◽  
Detection And Quantification ◽  
Routine Quality Control

Doxycycline, an oral antimicrobial, does not present a sustainable analytical method described in the literature using liquid chromatography. A new and efficient method was developed and validated for the quantification of doxycycline tablets by HPLC-UV. Its aim is the contribution to the green analytical chemistry since it has low use of organic solvent and low production of toxic waste. The HPLC-UV method used a mixture of purified water + 0.5 % acetic acid and ethanol (40:60, v/v). The flow rate was 0.8 mL min-1, C18 Luna column, 20 μL of injected volumes at 275 nm. The samples were prepared in purified water and the method was linear over the concentration range of 20–200 μg mL-1 (r = 0.9997) with limits of detection and quantification of 1.08 and 3.27 μg mL-1, respectively. The precision of the method showed RSD 0.50 % (intra-assay), 2.35 % (inter-assay) and 1.13 % (between analysts). The accuracy of the method was determined by standard recovery and it was 99.85 %. The DOX tablets were subjected to oxidative, acid, basic, neutral and photolytic degradation and it showed be stability indicative. Statistical analysis provided reliable, safety and reproducible results. The method is considered linear, selective, precise, accurate, robust, indicative of stability and safe to be used in routine quality control analyzes for determination and quantification of doxycycline in tablets. The proposed method is an ecologically correct alternative for the evaluation of doxycycline tablets.

Green analytical chemistry in the determination of organic pollutants in the aquatic environment

2010 ◽  
Vol 29 (11) ◽  
pp. 1347-1362 ◽  
Author(s):  
Marinella Farré ◽  
Sandra Pérez ◽  
Carlos Gonçalves ◽  
M.F. Alpendurada ◽  
Damià Barceló
Keyword(s):  
Analytical Chemistry ◽  
Organic Pollutants ◽  
Aquatic Environment ◽  
Green Analytical Chemistry

scholarly journals Rapid Analysis of Milk Using Low-Cost Pocket-Size NIR Spectrometers and Multivariate Analysis

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1090 ◽  
Author(s):  
Jordi Riu ◽  
Giulia Gorla ◽  
Dib Chakif ◽  
Ricard Boqué ◽  
Barbara Giussani
Keyword(s):  
Analytical Chemistry ◽  
Near Infrared ◽  
Multivariate Calibration ◽  
Low Cost ◽  
Analytical Data ◽  
Acquisition Time ◽  
Green Analytical Chemistry ◽  
Data Acquisition Time ◽  
Commercial Milk ◽  
New Generation

The miniaturisation of analytical devices, reduction of analytical data acquisition time, or the reduction of waste generation throughout the analytical process are important requirements of modern analytical chemistry, and in particular of green analytical chemistry. Green analytical chemistry has fostered the development of a new generation of miniaturized near-infrared spectroscopy (NIR) spectrometric systems. However, one of the drawbacks of these systems is the need for a compromise between the performance parameters (accuracy and sensitivity) and the aforementioned requirements of green analytical chemistry. In this paper, we evaluated the capabilities of two recently developed portable NIR instruments (SCiO and NeoSpectra) to achieve a rapid, simple and low-cost quantitative determination of commercial milk macronutrients. Commercial milk samples from Italy, Switzerland and Spain were chosen, covering the maximum range of variability in protein, carbohydrate and fat content, and multivariate calibration was used to correlate the recorded spectra with the macronutrient content of milk. Both SCiO and NeoSpectra can provide a fast and reliable analysis of fats in commercial milk, and they are able to correctly classify milk according to fat level. SCiO can also provide predictions of protein content and classification according to presence or absence of lactose.

scholarly journals Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples

2017 ◽  
Vol 67 (3) ◽  
pp. 373-384 ◽  
Author(s):  
Jumah Masoud Mohammad Salmani ◽  
Xue Wu ◽  
Joe Antony Jacob ◽  
Rong Fu ◽  
Baoan Chen
Keyword(s):  
Standard Sample ◽  
Drug Distribution ◽  
Rat Plasma ◽  
Hplc Method ◽  
Standard Samples ◽  
Factors Affecting ◽  
Highly Hydrophobic ◽  
Plasma Compatibility

AbstractIn the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation of standard samples and factors affecting drug distribution. A preparation procedure was devised to simulate the conditions the drug is expected to experiencein vivowhile pointing to the shortcomings of previously published methods. The method was validated according to the FDA regulations and showed to be highly efficient and capable of extracting the drug and IS from the plasma accurately and precisely within the specified range of 50–500 ng mL−1. Further, the standard sample preparation of this method can be used as a guideline for other methods, particularly when highly hydrophobic drugs with considerable protein binding are involved and could be valuable in the field of bioanalysis to improve the reliability of methods.

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