scholarly journals Biomanufacturing of Value-Added Products from Oils or Fats: A Case Study of Yarrowia lipolytica

2020 ◽  
Author(s):  
Na Liu ◽  
Ya-Hue Soong ◽  
Iman Mirzaee Kakhki ◽  
Andrew Olson ◽  
Peng Yu ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Value Added ◽  
Value Added Products

scholarly journals Bioconversion of biomass: a case study of ligno-cellulosics bioconversions in solid state fermentation

1998 ◽  
Vol 41 (4) ◽  
pp. 379-390 ◽  
Author(s):  
Ashok Pandey ◽  
Carlos R. Soccol
Keyword(s):  
Solid State ◽  
Sugarcane Bagasse ◽  
Solid State Fermentation ◽  
Value Added ◽  
Biological Processes ◽  
Lignocellulosic Residues ◽  
Recent Developments ◽  
Feed Enzymes ◽  
Value Added Products

Lignocellulosic residues obtained from crops cultivation form useful sources to be used as substrate for bioconversion processes. Sugarcane bagasse, which is a complex substrate obtained from the processing of sugar cane, is an important biomass among such sources. Due to its abundant availability, it can serve as an ideal substrate for microbial processes for the production of value added products. This paper reviews recent developments on biological processes developed on production of various products in solid state fermentation using sugarcane bagasse as the substrate and describes production of protein enriched feed, enzymes, amino acid, organic acids and compounds of pharmaceutical importance, etc. through microbial means.

scholarly journals Valorization of raw glycerol and crustacean waste into value added products by Yarrowia lipolytica

2017 ◽  
Vol 243 ◽  
pp. 57-68 ◽  
Author(s):  
Sara Magdouli ◽  
Tayssir Guedri ◽  
Rouissi Tarek ◽  
Satinder Kaur Brar ◽  
Jean François Blais
Keyword(s):  
Yarrowia Lipolytica ◽  
Value Added ◽  
Raw Glycerol ◽  
Value Added Products

scholarly journals α-Farnesene production from lipid by engineered Yarrowia lipolytica

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Yinghang Liu ◽  
Zhaoxuan Wang ◽  
Zhiyong Cui ◽  
Qingsheng Qi ◽  
Jin Hou
Keyword(s):  
Oleic Acid ◽  
Yarrowia Lipolytica ◽  
Edible Oils ◽  
Economic Value ◽  
Value Added ◽  
Waste Cooking Oil ◽  
Limiting Factor ◽  
Cell Factory ◽  
Microbial Cell Factory ◽  
Value Added Products

AbstractProducing high value-added products from waste lipid feedstock by microbial cell factory has great advantages to minimize the pollution as well as improve the economic value of wasted oils and fats. Yarrowia lipolytica is a non-conventional oleaginous yeast and can grow on a variety of hydrophobic substrates. In this study, we explored its ability to synthesize α-farnesene, an important sesquiterpene, using lipid feedstock. Based on the α-farnesene production strain, we constructed previously, we identified that Erg12 was the key limiting factor to further increase the α-farnesene production. The α-farnesene production was improved by 35.8% through increasing the copy number of ERG12 and FSERG20 on oleic acid substrate. Expression of heterologous VHb further improved α-farnesene production by 12.7%. Combining metabolic engineering with the optimization of fermentation conditions, the α-farnesene titer and yield reached 10.2 g/L and 0.1 g/g oleic acid, respectively, in fed-batch cultivation. The α-farnesene synthesis ability on waste cooking oil and other edible oils were also explored. Compared with using glucose as carbon source, using lipid substrates obtained higher α-farnesene yield and titer, but lower by-products accumulation, demonstrating the advantage of Y. lipolytica to synthesize high value-added products using lipid feedstock.

scholarly journals A teaching protocol demonstrating the use of EasyClone and CRISPR/Cas9 for metabolic engineering of Saccharomyces cerevisiae and Yarrowia lipolytica

2019 ◽  
Author(s):  
N Milne ◽  
L R R Tramontin ◽  
I Borodina
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Yarrowia Lipolytica ◽  
Yeast Species ◽  
Production Capacity ◽  
Value Added ◽  
Phenotypic Screen ◽  
Rate Limiting ◽  
Β Carotene

ABSTRACT We present a teaching protocol suitable for demonstrating the use of EasyClone and CRISPR/Cas9 for metabolic engineering of industrially relevant yeasts Saccharomyces cerevisiae and Yarrowia lipolytica, using β-carotene production as a case study. The protocol details all steps required to generate DNA parts, transform and genotype yeast, and perform a phenotypic screen to determine β-carotene production. The protocol is intended to be used as an instruction manual for a two-week practical course aimed at MSc and PhD students. The protocol details all necessary steps for students to engineer yeast to produce β-carotene and serves as a practical introduction to the principles of metabolic engineering including the concepts of boosting native precursor supply and alleviating rate-limiting steps. It also highlights key differences in the metabolism and heterologous production capacity of two industrially relevant yeast species. The protocol is divided into daily experiments covering a two week period and provides detailed instructions for every step meaning this protocol can be used ‘as is’ for a teaching course or as a case study for how yeast can be engineered to produce value-added molecules.

scholarly journals The Practice of Co-Production through Biocultural Design: A Case Study among the Bribri People of Costa Rica and Panama

2020 ◽  
Vol 12 (17) ◽  
pp. 7120
Author(s):  
Mariana Rodríguez Valencia
Keyword(s):  
Costa Rica ◽  
Social Relationships ◽  
Sustainability Science ◽  
Scientific Literature ◽  
Value Added ◽  
Agroforestry Systems ◽  
Cacao Butter ◽  
Biocultural Heritage ◽  
Value Added Products

Research in co-production has given rise to a rich scientific literature in sustainability science. The processes by which co-production occurs are not well documented. Here, I present my work with the Bribri people to undertake a biocultural design project. Biocultural design is a process that begins with understanding participants’ aspirations to support their livelihoods. The process is collaborative, imagining ideas and executing products and services by drawing upon the capabilities of the participant’s biocultural heritage. In the Bribri territory, the biocultural heritage associated with cacao agroforestry systems is considered significant for Bribri livelihoods. Bribri people’s aspirations to grow cacao go beyond increasing cacao yields and include the respect for cultural teachings and social relationships. The participants of this project designed cacao value-added products (e.g., cacao jam, cacao butter) and services (e.g., showcase farm) by identifying viable ways to execute their ideas. Biocultural design offers a guide to co-imagine and co-execute ideas to solve specific problems and contributes to the practice of co-production by offering an approach that recognizes the value of science, while respecting the knowledge, aspirations and values of other actors.

scholarly journals Synthesis of A Sustainable Sago-Based Value Chain via Fuzzy Optimisation Approach

2018 ◽  
Vol 152 ◽  
pp. 01004 ◽  
Author(s):  
Jeffrey Hong Seng Chong ◽  
Yoke Kin Wan ◽  
Viknesh Andiappan
Keyword(s):  
Waste Management ◽  
Value Chain ◽  
Value Added ◽  
Extraction Process ◽  
Sago Starch ◽  
Industrial Case Study ◽  
Industrial Practice ◽  
Sago Bark ◽  
Value Added Products

Sago starch is one of the staple foods for human, especially in Asia’s Region. It can be produced via sago starch extraction process (SSEP). During the SSEP, several types of sago wastes are generated such as sago fiber (SF), sago bark (SB) and sago wastewater (SW). With the increase in production of existing factories and sago mills, the sago industrial practice in waste disposal management is gaining more attention, thus implementation of effective waste management is vital. One of the promising ways to have effective waste management is to create value out of the sago wastes. In a recent study, sago-based refinery, which is a facility to convert sago wastes into value-added products (e.g., bio-ethanol and energy) was found feasible. However, the conversion of other value added products from sago wastes while considering the environmental impact has not been considered in sago value chain. Therefore, an optimum sago value chain, which involved conversion activities of sago wastes into value-added products, is aimed to be synthesised in this work. The optimum sago value chain will be evaluated based on profit and carbon emissions using fuzzy-based optimisation approach via a commercial optimisation software, Lingo 16.0. To illustrate the the developed approach, an industrial case study has been solved in this work.

scholarly journals Inherent Safety Analysis and Sustainability Evaluation of Chitosan Production from Shrimp Exoskeleton in Colombia

Water ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 553
Author(s):  
Antonio Zuorro ◽  
Kariana Andrea Moreno-Sader ◽  
Ángel Darío González-Delgado
Keyword(s):  
Return On Investment ◽  
Value Added ◽  
Safety Analysis ◽  
Main Process ◽  
Inherent Safety ◽  
Safety Index ◽  
Sustainability Evaluation ◽  
Shrimp Industry ◽  
Value Added Products

Waste valorization strategies are key to achieve more sustainable production within the shrimp industry. The crustacean exoskeletons can be potentially used to obtain value-added products such as chitosan. A comprehensive analysis including both safety and sustainability aspects of chitosan production from shrimp shells is presented in this study. The inherent safety analysis and sustainability evaluation was performed using the Inherent Safety Index (ISI) methodology and the Sustainable Weighted Return on Investment Metric (SWROIM), respectively. The process was designed for a processing capacity of 57,000 t/year. The return on investment (%ROI), potential environmental impact (PEI output), exergy efficiency, and the total inherent safety index (ITI) were used as indicators to evaluate process sustainability. The total inherent safety index was estimated at 25 indicating that the process is inherently unsafe. The main process risks were given by handling of flammable substances, reactivity, and inventory subindices. The overall sustainability evaluation showed a SWROIM of 36.33% indicating that the case study showed higher weighted performance compared to the return on investment metric of 18.08%.

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