Effects of anoxia on 31P NMR spectra of Phycomyces blakesleeanus during development

The method of 31P NMR spectroscopy was used to investigate the effects of anoxia on Phycomyces blakesleea?nus mycelium during development. The greatest changes were recorded in the PPc, NADH, and ?-ATP signals. Decrease of PPc signal intensity is due to chain length reduction and reduction in number of PPn molecules. Smaller decrease of ?-ATP compared to ?-ATP signal intensity can be attributed to maintenance of ATP concentration at the expense of PPn hydrolysis. Sensitivity to anoxia varies with the growth stage. It is greatest in 32-h and 44-h mycelium, in which PPn is used as an additional energy source, while the smallest effect was noted for 36-h fungi.

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Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus Phycomyces blakesleeanus

Archives of Biological Sciences ◽

10.2298/abs1402847s ◽

2014 ◽

Vol 66 (2) ◽

pp. 847-857

Author(s):

Marina Stanic ◽

M. Zivic ◽

Mirzeta Hadzibrahimovic ◽

Aleksandra Pajdic ◽

S. Krizak ◽

...

Keyword(s):

Nmr Spectroscopy ◽

31P Nmr ◽

31P Nmr Spectroscopy ◽

Antimycin A ◽

Phosphate Metabolism ◽

Phycomyces Blakesleeanus ◽

Tightly Coupled ◽

A Cell ◽

Inorganic Phosphates

The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxidase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by 31P NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the effect of cyanide on mitochondria.

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Quantitative Determination of Krill Oil Composition by ATR-FTIR, NIR, and 31P NMR Spectroscopy

10.21748/am20.144 ◽

2020 ◽

Author(s):

Ashraf Ismail ◽

Sanaz Molaye Moghaddam ◽

Jean-Pierre MetabanzoulouSarya Aziz ◽

Jacqueline Sedman ◽

Mazen Bahadi

Keyword(s):

Nmr Spectroscopy ◽

Quantitative Determination ◽

31P Nmr ◽

31P Nmr Spectroscopy ◽

Krill Oil ◽

Oil Composition

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Enzymatic decomposition of a locked nucleoside phosphoramidate monitored by 31P NMR spectroscopy

Collection Symposium Series ◽

10.1135/css201414356 ◽

2014 ◽

Author(s):

Eliška Procházková ◽

Hubert Hřebabecký ◽

Radim Nencka ◽

Martin Dračínský

Keyword(s):

Nmr Spectroscopy ◽

31P Nmr ◽

31P Nmr Spectroscopy

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Effect of Dietary Magnesium on Post Mortem Phosphocreatine Utilization in Skeletal Muscle of Swine: A Non-Invasive Study Using 31P-NMR Spectroscopy

Acta Veterinaria Scandinavica ◽

10.1186/bf03548184 ◽

1993 ◽

Vol 34 (4) ◽

pp. 397-404

Author(s):

B. Moesgaard ◽

I. Errebo Larsen ◽

B. Quistorff ◽

I. Therkelsen ◽

V. Grøsfjeld Christensen ◽

...

Keyword(s):

Skeletal Muscle ◽

Nmr Spectroscopy ◽

31P Nmr ◽

31P Nmr Spectroscopy ◽

Post Mortem ◽

Non Invasive ◽

Dietary Magnesium

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Unravelling the dissolution mechanism of polyphosphate glasses by 31P NMR spectroscopy: ligand competition and reactivity of intermediate complexes

Dalton Transactions ◽

10.1039/d0dt03381b ◽

2021 ◽

Author(s):

Dahiana Andrea Avila Salazar ◽

Peter Bellstedt ◽

Atsuhiro Miura ◽

Yuki Oi ◽

Toshihiro Kasuga ◽

...

Keyword(s):

Nuclear Magnetic Resonance ◽

Solid State ◽

Nmr Spectroscopy ◽

Biomedical Applications ◽

Structural Changes ◽

31P Nmr ◽

Dissolution Mechanism ◽

31P Nmr Spectroscopy ◽

Glass Dissolution ◽

Polyphosphate Glasses

Phosphate glass dissolution can be tailored via compositional and subsequent structural changes, which is of interest for biomedical applications such as therapeutic ion delivery. Here, solid-state 31P nuclear magnetic resonance...

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