scholarly journals Analysis of pretreatments of sugar beet shreds for bioethanol production in respect of cellulose hydrolysis and waste flows

2011 ◽  
pp. 223-230
Author(s):  
Darjana Ivetic ◽  
Vesna Vasic ◽  
Marina Sciban ◽  
Mirjana Antov
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugar Beet ◽  
Reducing Sugars ◽  
Cellulose Hydrolysis ◽  
Raw Material ◽  
Bioethanol Production ◽  
Lignin Removal ◽  
Chemical Pretreatments ◽  
Hydrolysis Of

This paper analyzes some chemical pretreatments of sugar beet shreds concerning generated waste flows and yield of reducing sugars obtained by enzymatic hydrolysis of pretreated material. Waste flows produced in pretreatments of sugar beet shreds originated from pectin and lignin removal from raw material. Suitability of substrates prepared in single and two-step pretreatment procedure for enzymatic hydrolysis was determined based on the yield of reducing sugars released by cellulase action on them, while different possibilities of processing of wastewaters were discussed based on the characteristic of waste flows.

scholarly journals The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds

2018 ◽  
pp. 1-9
Author(s):  
Mirjana Antov ◽  
Aleksandar Fistes
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugar Beet ◽  
Reducing Sugars ◽  
Cellulose Hydrolysis ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Untreated Material ◽  
Hydrolysis Of Cellulose ◽  
Beta Glucosidase ◽  
Hydrolysis Of

Sugar beet shreds were pretreated by hydrothermal procedure to investigate the effect of beta-glucosidase supplementation at different substrate loading on the rate of cellulose hydrolysis. Cellulose in the hydrothermally pretreated substrate was more efficiently hydrolyzed by enzymes than in untreated material, resulting in more than two times higher release of reducing sugars. In the investigated range of solids load, supplementation of fungal cellulases cocktail by beta-glucosidase increased production of reducing sugars from substrates, while 0.25 U/g was sufficient to achieve the highest effect under applied conditions.

scholarly journals BIOETHANOL PRODUCTION FROM COCONUT HUSK USING THE WET GAMMA IRADIATION METHOD

2021 ◽  
Vol 14 (2) ◽  
pp. 43
Author(s):  
Putra Oktavianto ◽  
Risdiyana Setiawan ◽  
Ilhami Ariyanti ◽  
Muhammad Fadhil Jamil
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cellulose Hydrolysis ◽  
Raw Material ◽  
Bioethanol Production ◽  
Coconut Husk ◽  
Hydrolysis Process ◽  
Hydrolysis Of Cellulose ◽  
Ethanol Ethanol ◽  
Hydrolysis Of ◽  
Low Dosage

BIOETHANOL PRODUCTION FROM COCONUT HUSK USING the WET GAMMA IRRADIATION METHOD. The use of coconut husk has only been used as a material for making handicrafts such as ropes, brooms, mats, and others or just burned. The combustion of coconut husk can cause air pollution. In fact, coconut husk can be used as a raw material for bioethanol production so that the beneficial value of coconut husk will also increase. One way of bioethanol production from coconut husk is by irradiating the coconut husk. The coconut husk irradiation technique to be carried out in this study is the wet irradiation technique. Wet irradiation is carried out to accelerate the process of bioethanol production because at the time of irradiation, cellulose has been hydrolyzed and glucose has been formed so that it is more efficient in time and use of the material so that the cellulose hydrolysis process is not necessary. The coconut husk samples were wet because they were mixed with 4% NaOH and were irradiated using a gamma irradiator from STTN-BATAN Yogyakarta with a dose of 30 kGy and 50 kGy and 0 kGy (or without irradiation). Then the sample is fermented with the fungus Saccharomyces Cerevisiae from tape yeast to form ethanol. Ethanol is purified and then analyzed for concentrations using pycnometric and refractometric methods. The result is that the highest ethanol content is without irradiation (0 kGy), this is due to the low dosage used. However, the main point in this wet method research is evidence of hydrolysis of cellulose by the formation of gluoxane after irradiated wet coconut husk, and with Fehling A and B analysis, brown deposits are seen proving that glucose has been formed.

scholarly journals Effect of Combinative Pretreatments on Cellulose-to-Glucose Conversion of Empty Palm Fruit Bunch (EFB)

2014 ◽  
Vol 7 ◽  
pp. 81-85
Author(s):  
Yakindra Prasad Timilsena ◽  
Nicolas Brosse
Keyword(s):  
Enzymatic Hydrolysis ◽  
Reducing Sugars ◽  
Dilute Acid ◽  
Palm Fruit ◽  
Step Counter ◽  
Lignin Removal ◽  
One Step ◽  
Total Reducing Sugars ◽  
Hydrolysis Of ◽  
Sugar Conversion

Various methods of pretreatments were investigated to their effect on cellulose to glucose conversion efficiency on enzymatic hydrolysis of a tropical agro-industrial waste residue (empty palm fruit bunch, EFB). Four different kinds of combinative pretreatments (autohydrolysis with and without naphthol, dilute acid prehydrolysis, soda prehydrolysis and enzymatic prehydrolysis) were tested for delignification during the first and the second steps of pretreatment. Each prehydrolysis step was seconded by the organosolv delignification with the same conditions of pretreatment. It was observed that all the combinative methods were far more efficient in delignification and enzymatic hydrolysis ability as compared to its one step counter parts. The combinative pretreatment method involving dilute acid prehydrolysis followed by organosolv delignification revealed the best result with respect to lignin removal and enzymatic hydrolysis. The resultant pulp contained very low Klason lignin (~5%) with high sugar conversion ratio (64% total reducing sugars). DOI: http://dx.doi.org/10.3126/jfstn.v7i0.10613   J. Food Sci. Technol. Nepal, Vol. 7 (81-85), 2012  

scholarly journals FEASIBILITY TO PRODUCING SECOND GENERATION BIOETHANOL IN BOLIVIA

2020 ◽  
Vol 51 (1) ◽  
pp. 57-61
Author(s):  
Antonio Gil ◽  
M. Beltran Siñani
Keyword(s):  
Enzymatic Hydrolysis ◽  
Environmental Impact ◽  
Impact Evaluation ◽  
Second Generation ◽  
Reducing Sugars ◽  
Agricultural Waste ◽  
Agricultural Crops ◽  
Bioethanol Production ◽  
Second Generation Bioethanol ◽  
Hydrolysis Of

The bioethanol that is produced worldwide is mostly obtained from agricultural crops such as sugarcane and corn. However, it has negative environmental effects, so the option of producing bioethanol from agricultural waste arises. This work evaluates the feasibility to produce second generation bietanol from oranges residues (peel and bagasse) produced in the province of Chapare, Bolivia. The estimation is carried out from the reducing sugars, determined by the DNS method, individual sugars, determined by HPLC, produced by acidic and enzymatic hydrolysis of the residues. Similarly, the amount of ethanol produced by fermentation of the samples is quantified. Regarding the results obtained, the best alternative in terms of bioethanol production is the enzymatic hydrolysis. An economic and environmental impact evaluation are also included considering the production of bioethanol from real orange residues.

scholarly journals ENZYMATIC HYDROLYSIS OF SOLUBLE CELLULOSE DERIVATIVES AS MEASURED BY CHANGES IN VISCOSITY

1950 ◽  
Vol 33 (5) ◽  
pp. 601-628 ◽  
Author(s):  
Hillel S. Levinson ◽  
Elwyn T. Reese
Keyword(s):  
Enzymatic Hydrolysis ◽  
Intrinsic Viscosity ◽  
Reducing Sugars ◽  
Enzyme System ◽  
Molecular Size ◽  
Cellulose Hydrolysis ◽  
Reducing Sugar ◽  
Cellulose Derivatives ◽  
Enzyme Preparations ◽  
Hydrolysis Of

Observation of changes in fluidity is presented as a method for following the enzymatic hydrolysis of soluble cellulose derivatives. The activity of different cell-free enzyme preparations may be compared by this method, providing certain precautions are observed. In general, results obtained by use of the fluidity method are similar to those obtained using the reducing sugar technique, indicating that the same enzyme system is measured by the two methods. Changes in the DP of the substratum may be followed within certain limits of molecular size. Results indicate that a random splitting of CMC occurs during enzymatic hydrolysis, with a concomitant decrease in intrinsic viscosity and an increase in reducing sugars. Certain inadequacies of the cellulose-cellobiose-glucose theory, together with more recent findings, have led to the postulation of an alternate explanation of the mechanism of cellulose hydrolysis.

Improving the efficiency of enzymatic hydrolysis of Eucalyptus residues with a modified aqueous ammonia soaking method

2018 ◽  
Vol 33 (2) ◽  
pp. 165-174 ◽  
Author(s):  
Dan Huo ◽  
Qiulin Yang ◽  
Guigan Fang ◽  
Qiujuan Liu ◽  
Chuanling Si ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Enzymatic Hydrolysis ◽  
Aqueous Ammonia ◽  
Pulp Mill ◽  
Fiber Surface ◽  
Raw Material ◽  
Sem Images ◽  
Aqueous Ammonia Soaking ◽  
Guaiacyl Lignin ◽  
Hydrolysis Of

Abstract Eucalyptus residues from pulp mill were pretreated with aqueous ammonia soaking (AAS) method to improve the efficiency of enzymatic hydrolysis. The optimized condition of AAS was obtained by response surface methodology. Meanwhile, hydrogen peroxide was introduced into the AAS system to modify the AAS pretreatment (AASP). The results showed that a fermentable sugar yield of 64.96 % was obtained when the eucalypt fibers were pretreated at the optimal conditions, with 80 % of ammonia (w/w) for 11 h and keeping the temperature at 90 °C. In further research it was found that the addition of H2O2 to the AAS could improve the pretreatment efficiency. The delignification rate and enzymatic digestibility were increased to 64.49 % and 73.85 %, respectively, with 5 % of hydrogen peroxide being used. FTIR analysis indicated that most syringyl and guaiacyl lignin and a trace amount of xylan were degraded and dissolved during the AAS and AASP pretreatments. The CrI of the raw material was increased after AAS and AASP pretreatments, which was attributed to the removal of amorphous portion. SEM images showed that microfibers were separated and explored from the initial fiber structure after AAS pretreatment, and the AASP method could improve the destructiveness of the fiber surface.

Optimization of steam pretreatment conditions for enzymatic hydrolysis of poplar wood

Holzforschung ◽  
2011 ◽  
Vol 65 (4) ◽  
Author(s):  
Fokko Schütt ◽  
Jürgen Puls ◽  
Bodo Saake
Keyword(s):  
Enzymatic Hydrolysis ◽  
Raw Material ◽  
Steam Pretreatment ◽  
Alkaline Extraction ◽  
Poplar Wood ◽  
Steam Refining ◽  
Severity Factor ◽  
Short Rotation ◽  
Pretreatment Conditions ◽  
Hydrolysis Of

Abstract Steam refining was investigated as a pretreatment for enzymatic hydrolysis of poplar wood from a short rotation plantation. The experiments were carried out without debarking to use an economically realistic raw material. Steam refining conditions were varied in the range of 3–30 min and 170–220°C, according to a factorial design created with the software JMP from SAS Institute Inc., Cary, NC, USA. Predicted steaming conditions for highest glucose and xylose yields after enzymatic hydrolysis were at 210°C and 15 min. Control tests under the optimized conditions verified the predicted results. Further pretreatments without bark showed that the enzymes were not significantly inhibited by the bark. The yield of glucose and xylose was 61.9% of theoretical for the experiments with the whole raw material, whereas the yield for the experiments without bark was 63.6%. Alkaline extraction of lignin from the fibers before enzymatic hydrolysis resulted in an increase of glucose yields from mild pretreated fibers and a decrease for severe pretreated fibers. The extracted lignin had a high content of xylose of up to 14% after very mild pretreatments. On the other hand, molecular weights of the extracted lignin increased substantially after pretreatments with a severity factor above 4. Hence, alkaline extraction of the lignin seems only attractive in a narrow range of steaming conditions.

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