scholarly journals Histochemical analysis of skeletal muscular tissues of pigs according to genotype <i>MYF 4</i> (Short communication)

2009 ◽  
Vol 52 (4) ◽  
pp. 395-401
Author(s):  
P. Makovický ◽  
P. Makovický ◽  
M. Kačániová ◽  
S. Pavličová ◽  
P. Haščík
Keyword(s):  
Short Communication ◽  
Rectus Femoris ◽  
Polymerase Chain Reaction Method ◽  
Muscle Fibres ◽  
Triceps Brachii ◽  
Chain Reaction ◽  
Histochemical Analysis ◽  
Polymerase Chain ◽  
Muscular Tissues

Abstract. The results of histochemical analysis of 3 muscles – m. triceps brachii (MTB), m. longissimus thoracitus (MLT) and m. rectus femoris (MRF) – in 2 groups of pigs formed according to the genotypes MYF 4 are presented in this study. Determination of MYF 4 genotypes was performed by the polymerase chain reaction method (PCR). For histochemical analysis, 5 animals detected as homozygotes of the MYF 4-AA type and 5 animals of the heterozygous genotype AB were used out of the total 25 individual animals tested. The histochemical analysis demonstrated that homozygotes AA had larger fat cells on the average than heterozygotes AB in the three studied muscles, which was statistically significant (P<0.05). The percentage surface representation of interstitial tissues was higher in the studied muscles of heterozygotes MYF 4-AB. The volume of interstitial tissues was the highest in the MRF in both groups (myogenin – AA and AB). The average thickness of muscle fibres of the three studied muscles was higher in the homozygote genotype myogenin MYF 4-AA than in heterozygotes MYF 4-AB, which was statistically significant (P<0.05).

scholarly journals Histochemical analysis of skeletal muscular tissues of pigs according to genotype MYF4

2008 ◽  
Vol 24 (1-2) ◽  
pp. 117-126
Author(s):  
Vladimir Zimmermann ◽  
V. Kulísek ◽  
A. Copík ◽  
M. Odstrcil ◽  
Ondrej Debrecéni ◽  
...  
Keyword(s):  
Rectus Femoris ◽  
Muscle Fibres ◽  
Fat Cells ◽  
Average Thickness ◽  
Triceps Brachii ◽  
Surface Representation ◽  
Histochemical Analysis ◽  
Pcr Method ◽  
Muscular Tissues

The results of histochemical analysis of three muscles m. triceps brachii (MTB), m. longissimus thoracicus (MLT) and m. rectus femoris (MRF) of two groups of pigs created according to the genotypes MYF 4 are presented. Determination of MYF 4 genotypes was made by PCR method and for histochemical analysis was used 5 animals detected as homozygote MYF 4- AA type and 5 animals of heterozygote genotype myogenin-AB out of the total of 25 individual animals tested. The histochemical analysis proved that homozygotes AA have had bigger fat cells than heterozygotes AB in three studied muscles in average. The size of fat cells in MLT - 41.10?m or 38.50 ?m respectively dominated in both groups of animals. Percentage surface representation of interstitial tissues was higher in the studied muscles of heterozygote MYF 4-AB. The volume of ligaments was the highest in MRF (3.80% or 3.90% respectively) in both groups (myogenin - AA and AB). The average thickness was of three studied muscles muscle fibres higher at homozygote genotype myogenin-AA than in heterozygote myogenin-AB. The thickest fibres in both genotypes were in MRF (88.60 ?m, and 84.72 ?m respectively) and the lowest in MTB (73.30 and 69.40 ?m respectively). The highest values of muscle fibres thickness were detected in ?-White fibres. Their percentage surface representation corresponded to this in all three types of muscles of both studied genotype myogenin groups.

Determination of candida species from mouth and vagina by use of polymerase chain reaction method

2011 ◽  
Vol 44 (13) ◽  
pp. S130-S131
Author(s):  
Saeid Mahdavi Omran ◽  
Ramazan Rajabnia ◽  
Seyed Ali Asghar Sefidgar ◽  
Seddigheh Esmaeilzadeh ◽  
Maryam Ghasempour ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Species ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
Polymerase Chain

Rapid determination of vapA/vapB genotype in Rhodococcus equi using a differential polymerase chain reaction method

2004 ◽  
Vol 85 (4) ◽  
pp. 317-326 ◽  
Author(s):  
Christopher Oldfield ◽  
Hal Bonella ◽  
Lynne Renwick ◽  
Hilary I. Dodson ◽  
Grace Alderson ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rapid Determination ◽  
Polymerase Chain Reaction Method ◽  
Rhodococcus Equi ◽  
Chain Reaction ◽  
Reaction Method ◽  
Polymerase Chain

scholarly journals Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yudith Violetta Pamulang ◽  
Aris Haryanto
Keyword(s):  
Polymerase Chain Reaction ◽  
Peripheral Blood ◽  
Short Communication ◽  
Dna Amplification ◽  
Polymerase Chain Reaction Method ◽  
Molecular Sexing ◽  
Chain Reaction ◽  
Reaction Method ◽  
Three Samples ◽  
Polymerase Chain

Abstract. Pamulang YV, Haryanto A. 2021. Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method. Biodiversitas 22: 449-452. Sex determination is an important aspect in breeding, ecological studies, rearing and reproductive management on Kutilang (Pycnonotus sp.) birds. It is based on differences in intron lengths from DNA amplification of the Chromodomain Helicase DNA-binding (CHD) genes on the Z and W sex chromosomes. This study aims to compare the molecular bird sexing method on Pycnonotus sp. by PCR amplification derived from two different DNA sources. DNA was extracted from DNA 100 µL peripheral blood (1-2 drops) and three plucked feathers from each sample. The CHD genes were amplified using PCR method and visualized by electrophoresis on 1.5% agarose gel. The amplified DNA fragments were single and double DNA bands in the size of 300 bp for the CHD-Z and 400 bp for the CHD-W genes. Three samples were identified as males from the six Pycnonotus sp. while the other three were females. The molecular sexing of six Kultilang (Pycnonotus sp.) birds showed that three samples were males and three were females. Descriptive analysis of two different DNA sources showed that the DNA extracted from peripheral blood samples has better quality than DNA extracted from plucked feathers.

Sign in / Sign up

Export Citation Format

Share Document