scholarly journals Nattokinase production: Medium components and feeding strategy studies

2014 ◽  
Vol 20 (4) ◽  
pp. 541-547 ◽  
Author(s):  
Aydin Berenjian ◽  
Raja Mahanama ◽  
John Kavanagh ◽  
Fariba Dehghani ◽  
Younes Ghasemi
Keyword(s):  
Feeding Strategy ◽  
Scale Up ◽  
Fold Increase ◽  
Polynomial Equation ◽  
Fed Batch ◽  
Medium Components ◽  
Bacillus Subtilis Natto ◽  
Order Polynomial ◽  
Composite Face ◽  
Central Composite

In the present study, the effect of nutrients on nattokinase activity during the fermentation of Bacillus subtilis natto was investigated. The highest nattokinase activity of 587 U/mL was obtained in fermenter for a media consisting of yeast extract (6%, w/v), soy peptone (1.2%, w/v) and glycerol (6%, w/v). The second order polynomial equation was fitted to the results by using central composite face design. The polynomial model fitted the experimental data well with R2 = 0.939 and R2(Adj) = 0.861. In addition, contribution of fed-batch glycerol addition on the nattokinase production pathway was further investigated. Maximum nattokinase activity was resulted when 3% (w/v) glycerol was added to the fermentation media during the cell growth phase. Results demonstrate 25 fold increase of nattokinase activity compared to the batch and not optimized culture. This study provides valuable data on the key nutrients and feeding strategy for further investigations and scale up of nattokinase production process.

Enhanced Alkali Pretreatment of Narrow Leaves Cattail by Response Surface Methodology

2014 ◽  
Vol 875-877 ◽  
pp. 1637-1641
Author(s):  
Arrisa Sopajarn ◽  
Chayanoot Sangwichien
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Residence Time ◽  
Preliminary Test ◽  
Polynomial Equation ◽  
Alkali Concentration ◽  
Pretreatment Condition ◽  
Order Polynomial ◽  
Optimized Conditions ◽  
Central Composite

The purpose of this work is to develop a pretreatment process of lingo-cellulosic ethanol production from narrow leaves cattail (Typha angustifolia) by using alkali catalysis with the response surface methodology (RSM) as a central composite design (CCD). The first step, LiOH, NaOH, and KOH were used as catalytic alkali for preliminary test. Second, the suitable alkali from first step was selected to optimize of pretreatment condition of three independent variables (alkali concentration, temperature, and residence time) that varies at CCD five codes (-2, -1, 0, 1, 2). Sodium hydroxide (NaOH) is the proper alkali because it could increase cellulose more than KOH and nearby LiOH while it is cheapest. RSM result shows the optimized pretreatment condition based on cellulose increased which obtained from this study that is NaOH 5 % w/v at 100 °C and residence time for 120 min. Beside, this condition was analyzed using an ANOVA with a second order polynomial equation after eliminated non-significant terms. At the optimized conditions, cellulose increased, hemicellulose decreased and weight recovery were achieved 77.81%, 80.59, and 41.65%, respectively. Moreover, the model was reasonable to predict the response of strength with less than 5% error.

scholarly journals Medium Optimization for the Production of Fibrinolytic Enzyme byPaenibacillussp. IND8 Using Response Surface Methodology

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Ponnuswamy Vijayaraghavan ◽  
Samuel Gnana Prakash Vincent
Keyword(s):  
Fold Increase ◽  
Fibrinolytic Enzyme ◽  
Dihydrogen Phosphate ◽  
Key Factors ◽  
Sodium Dihydrogen Phosphate ◽  
Fibrinolytic Enzymes ◽  
Order Polynomial ◽  
Full Factorial ◽  
Sodium Dihydrogen ◽  
Central Composite

Production of fibrinolytic enzyme by a newly isolatedPaenibacillussp. IND8 was optimized using wheat bran in solid state fermentation. A 25full factorial design (first-order model) was applied to elucidate the key factors as moisture, pH, sucrose, yeast extract, and sodium dihydrogen phosphate. Statistical analysis of the results has shown that moisture, sucrose, and sodium dihydrogen phosphate have the most significant effects on fibrinolytic enzymes production (P<0.05). Central composite design (CCD) was used to determine the optimal concentrations of these three components and the experimental results were fitted with a second-order polynomial model at 95% level (P<0.05). Overall, 4.5-fold increase in fibrinolytic enzyme production was achieved in the optimized medium as compared with the unoptimized medium.

scholarly journals Seed train process intensification strategy offers potential for rapid, cost-effective scale-up of biosimilars manufacturing

2021 ◽  
Vol 20 ◽  
Author(s):  
Rajib Malla ◽  
Dhaval Shah ◽  
Chinmay Gajendragadkar ◽  
Vijayalakshmi Vamanan ◽  
Deepak Singh ◽  
...  
Keyword(s):  
Feeding Strategy ◽  
Scale Up ◽  
Control Process ◽  
Process Intensification ◽  
Cost Effective ◽  
Fold Increase ◽  
Exponential Growth Phase ◽  
Production Scale ◽  
Seed Train ◽  
Process Transfer

A perfusion approach at N-1, where cells stay in the exponential growth phase throughout the entire culture duration, is becoming more common as a strategy for process intensification. This is because the higher cell densities it generates allows manufacturers to skip seed stages and reduce process transfer time through multiple bioreactor sizes, thus providing more cost-effective biologics production in smaller facilities. However, this N-1 perfusion approach requires optimization. In this article, we describe the development and proof-of-concept studies with single-use rocking motion perfusion bioreactors in which we have achieved a ten-fold increase in viable cell count in N-1 seed stage, compared to the fed-batch control process, in just 6–8 days. We also mention in detail how we inoculated a 50 L bioreactor production run using this intensified seed train and show comparable growth kinetics and yield with a control process, also at 50 L scale. Using this intensification approach in the future will help our manufacturing facility, the Biopharma Division of Intas Pharmaceuticals Ltd., reach 4000 L production-scale volumes with fewer process transfer steps, and without changing the feeding strategy or production bioreactors of our biologics’ portfolio.

Optimization of Soluble Organic Selenium Accumulation during Fermentation of Flammulina velutipes Mycelia

2012 ◽  
Vol 67 (11-12) ◽  
pp. 594-602
Author(s):  
Yunfeng Ma ◽  
Fu Xiang ◽  
Jun Xiang ◽  
Longjiang Yu
Keyword(s):  
Feeding Strategy ◽  
Logistic Function ◽  
Flammulina Velutipes ◽  
Batch Cultures ◽  
Organic Selenium ◽  
Medium Components ◽  
Inorganic Selenium ◽  
Essential Nutrient ◽  
Optimal Medium ◽  
Central Composite

Selenium is an essential nutrient with diverse physiological functions, and soluble organic selenium (SOS) sources have a higher bioavailability than inorganic selenium sources. Based on the response surface methodology and central composite design, this study presents the optimal medium components for SOS accumulation in batch cultures of Flammulina velutipes, i.e. 30 g/L glucose, 11.2 mg/L sodium selenite, and 1.85 g/L NH4NO3. Furthermore, logistic function model feeding was found to be the optimal feeding strategy for SOS accumulation during Flammulina velutipes mycelia fermentation, where the maximum SOS accumulation reached (4.63 ± 0.24) mg/L, which is consistent with the predicted value.

scholarly journals Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2833
Author(s):  
J. N. Bandal ◽  
V. A. Tile ◽  
R. Z. Sayyed ◽  
H. P. Jadhav ◽  
N. I. Wan Azelee ◽  
...  
Keyword(s):  
Catalytic Efficiency ◽  
Fold Increase ◽  
Soluble Starch ◽  
Statistical Optimization ◽  
Bacillus Sp ◽  
Bioprocess Design ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology. This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization. The use of Plackett–Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product. In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp. H7 by 16S rRNA. The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase. A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor. Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess. The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp. H7 on soluble starch was estimated to be 13.73 mL/s/mg.

scholarly journals Formulation and Mathematical Optimization of Controlled Release Calcium Alginate Micro Pellets of Frusemide

2013 ◽  
Vol 2013 ◽  
pp. 1-14 ◽  
Author(s):  
Amitava Ghosh ◽  
Prithviraj Chakraborty
Keyword(s):  
Sodium Alginate ◽  
Calcium Alginate ◽  
Mathematical Optimization ◽  
Extended Period ◽  
Cost Effective ◽  
Polynomial Equation ◽  
Chronic Hypertension ◽  
Order Polynomial ◽  
High Degree

Objective. Frusemide loaded calcium alginate micropellets, an oral microparticulate delivery system, was statistically optimized exhibiting prolonged therapeutic action minimizing its adverse effects.Methods. Ionotropic Gelation technique was adopted employing 32Factorial designs and keeping the entire process free from organic solvents. Physicochemical and the release characteristics of the prepared formulations were studied, keeping variations only in sodium alginate (primary polymer) and Acrycoat E30D (copolymer) dispersion.Result. Sodium alginate was predominant over Acrycoat E30D in all batches. Nonadditives or interaction was observed to be insignificant. Multiple regressions produced second-order polynomial equation, and the predictive results obtained were validated with high degree of correlation. Thein vivostudy applauded that optimized calcium alginate micropellets of frusemide can produce a much greater diuretic effect over an extended period of 24 hours.Conclusion. This study reveals that the potential of a single dose of the mathematically optimized micro pellets of frusemide formulation is sufficient in the management of peripheral edema and ascites in congestive heart failure and as well in the treatment of chronic hypertension, leading to better patient compliance, and can be produced with minimum experimentation and time, proving far more cost-effective formulation than the conventional methods of formulating dosage forms.

EVALUATION OF A NATURAL RUBBER SLEEVE STOPPER AS A CONTROLLED-RELEASE SUBSTRATE FOR USE IN STUDIES OF SEX-PHEROMONE-MEDIATED MATING DISRUPTION OF THE SPOTTED TENTIFORM LEAFMINER

1999 ◽  
Vol 131 (1) ◽  
pp. 137-149 ◽  
Author(s):  
R.M. Trimble ◽  
C.A. Tyndall ◽  
B.D. McGarvey
Keyword(s):  
Sex Pheromone ◽  
Natural Rubber ◽  
Mating Disruption ◽  
Polynomial Equation ◽  
Order Polynomial ◽  
Rubber Sleeve ◽  
Rate Of Evaporation ◽  
The Relationship ◽  
Linear Decline ◽  
Order Polynomial Equation

AbstractNatural rubber sleeve stoppers were impregnated with 10 mg of (E)-10-dodecen-1-yl-acetate, the major component of spotted tentiform leafminer, Phyllonorycter blancardella (F.), pheromone. In the laboratory, there was a linear decline in the amount of pheromone remaining on stoppers during 8 weeks of exposure to 10, 15, 20, and 25 °C. At 30 and 35 °C, the relationship was curvilinear and could be described using a second-order polynomial equation. The estimated rate of evaporation ranged from 0.03 mg/day at 10 °C to 0.08 mg/day at 25 °C; the rate of evaporation at 30 and 35 °C varied with the time since first exposure. There was a linear decline in the amount of pheromone remaining on stoppers during 8 weeks of exposure to fluctuating temperature regimes with average temperatures of 10 °C (i.e., 5–15 °C) and 20 °C (i.e., 15–25 °C). At a fluctuating regime with an average temperature of 30 °C (i.e., 25–35 °C), the relationship was curvilinear and could be described using a second-order polynomial equation. The estimated rate of evaporation was 0.02 and 0.09 mg/day at 5–15 and 15–25 °C, respectively; the estimated daily rate of evaporation at 25–35 °C varied with the time since first exposure. In an orchard, the estimated average rate of evaporation of pheromone from stoppers ranged from 0.05 to 0.31 mg/day and did not vary significantly with temperature. During the first 4 weeks of exposure in an orchard, the observed rate of evaporation was up to 4.4-fold greater than the rate predicted using the relationship between evaporation rate and constant temperatures observed in the laboratory. The potential for using natural rubber sleeve stoppers as controlled-release substrates in studies of sex-pheromone-mediated mating disruption of P. blancardella is discussed.

Sign in / Sign up

Export Citation Format

Share Document