Tissue toxicity of root canal sealers

The aim of this study was to evaluate tissue response to root canal sealers Tubuliseal and Sealapex. The sealers were freshly mixed and injected in the dorsal subcutaneous connective tissue of 12 Wistar rats.The observation periods were 7 days, 21days and 60 days. Four operative areas were formed ( 2 for test sealers, Tubliseal or Sealapex and 2 for control material) on each animal. Tissue sections were taken from selected sites. Each section included skin, subcutaneous connective tissue and underlying muscle tissue.All blocks were processed with the use of standardized histological procedures.The tissue reactions were studied under light microscopy. Different grades of tissue reaction to the tested materials were recorded as mild, moderate or severe inflammation. After seven days both root canal sealers showed severe inflammatory reaction of connective tissue in experimental animals.Tubuliseal caused prolonged moderate and mild inflammation. Sealapex caused mild inflammation which diminished at the end of the observation period. The results of this study demonstrated that Sealapex was better tolerated by tissue than Tubliseal.

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The response of subcutaneous connective tissue to newly developed calcium phosphate-based root canal sealers

International Endodontic Journal ◽

10.1111/j.1365-2591.2010.01836.x ◽

2011 ◽

Vol 44 (4) ◽

pp. 342-352 ◽

Cited By ~ 9

Author(s):

R. M. Khashaba ◽

M. M. Moussa ◽

N. B. Chutkan ◽

J. L. Borke

Keyword(s):

Calcium Phosphate ◽

Connective Tissue ◽

Root Canal ◽

Subcutaneous Connective Tissue ◽

Root Canal Sealers

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In vivo comparison of the biocompatibility of two root canal sealers implanted into the subcutaneous connective tissue of rats

Journal of Endodontics ◽

10.1016/s0099-2399(98)80082-4 ◽

1998 ◽

Vol 24 (2) ◽

pp. 82-85 ◽

Cited By ~ 37

Author(s):

Ioannis Kolokouris ◽

Nikolaos Economides ◽

Panagiotis Beltes ◽

Ioannis Vlemmas

Keyword(s):

Connective Tissue ◽

Root Canal ◽

Subcutaneous Connective Tissue ◽

Root Canal Sealers

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Tissue Response to a New Methacrylate-Based Root Canal Sealer: Preliminary Observations in the Subcutaneous Connective Tissue of Rats

Journal of Endodontics ◽

10.1097/00004770-200405000-00010 ◽

2004 ◽

Vol 30 (5) ◽

pp. 348-351 ◽

Cited By ~ 58

Author(s):

O ZMENER

Keyword(s):

Connective Tissue ◽

Root Canal ◽

Tissue Response ◽

Root Canal Sealer ◽

Subcutaneous Connective Tissue

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In vivo comparison of the biocompatibility of two root canal sealers implanted into the subcutaneous connective tissue of rats

Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology ◽

10.1016/j.tripleo.2006.11.025 ◽

2007 ◽

Vol 103 (5) ◽

pp. e88-e94 ◽

Cited By ~ 17

Author(s):

Edilson José Zafalon ◽

Marco Aurélio Versiani ◽

Cássio José Alves de Souza ◽

Camila Christian Gomes Moura ◽

Paula Dechichi

Keyword(s):

Connective Tissue ◽

Root Canal ◽

Subcutaneous Connective Tissue ◽

Root Canal Sealers

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Tissue response to an experimental calcium hydroxide-based endodontic sealer: a quantitative study in subcutaneous connective tissue of the rat

Dental Traumatology ◽

10.1111/j.1600-9657.1990.tb00393.x ◽

1990 ◽

Vol 6 (2) ◽

pp. 66-71 ◽

Cited By ~ 20

Author(s):

Osvaldo Zmener ◽

Maria B. Guglielmotti ◽

Romulo L. Cabrini

Keyword(s):

Connective Tissue ◽

Calcium Hydroxide ◽

Quantitative Study ◽

Tissue Response ◽

Subcutaneous Connective Tissue

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Histological analysis of the biocompatibility of three different calcium hydroxide-based root canal sealers

Journal of Applied Oral Science ◽

10.1590/s1678-77572006000500014 ◽

2006 ◽

Vol 14 (5) ◽

pp. 376-381 ◽

Cited By ~ 7

Author(s):

Heloisa Helena Pinho Veloso ◽

Roberto Alves do Santos ◽

Túlio Pessoa de Araújo ◽

Denise Piotto Leonardi ◽

Flares Baratto Filho

Keyword(s):

Calcium Hydroxide ◽

Study Design ◽

Inflammatory Reaction ◽

Root Canal ◽

Histological Analysis ◽

Subcutaneous Tissue ◽

Inflammatory Cells ◽

Control Groups ◽

Tissue Reactions ◽

Root Canal Sealers

OBJECTIVE: To histologically evaluate the behavior of the subcutaneous tissue of rats after placement of polyethylene tubes filled with calcium hydroxide-based root canal sealers (SealapexTM, ApexitTM e Sealer 26TM). STUDY DESIGN: The study employed 60 rats, which were divided into eight groups, being four experimental groups with 10 rats each, and four control groups with five rats. Analysis was conducted at 48 hours, 7, 21 and 60 days. RESULTS: Histological analysis revealed that all sealers were irritant; however, the intensity varied between each sealer and as a function of time. At the initial periods (48 hours and seven days), all materials demonstrated similar outcomes, with inflammatory reaction from severe to moderate, respectively. At the final periods (21 and 60 days), the tissue reactions to the implants containing SealapexTM displayed an active status yet with regression, compared to the ApexitTM and Sealer 26TM. The control groups exhibited less inflammatory cells than the experimental groups, keeping a reaction status with regression. CONCLUSIONS: The results achieved in this study revealed that the SealapexTM sealer was the most compatible, followed by ApexitTM and Sealer 26TM.

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A Comparative Study of Biocompatibility in Rat Connective Tissue of a New Mineral Trioxide Compound (Theracal) versus MTA and a Bioactive G3 Glass

Journal of Clinical Medicine ◽

10.3390/jcm10122536 ◽

2021 ◽

Vol 10 (12) ◽

pp. 2536

Author(s):

Jesús Mena-Álvarez ◽

Cristina Rico-Romano ◽

Carlos Gutiérrez-Ortega ◽

Pablo Arias-Sanz ◽

Javier Castro-Urda

Keyword(s):

Connective Tissue ◽

Inflammatory Reaction ◽

Direct Contact ◽

Wistar Rats ◽

Tissue Response ◽

New Mineral ◽

Exact Test ◽

Dorsal Area ◽

Subcutaneous Connective Tissue ◽

Hematoxylin Eosin

The aim of this paper was to assess the differences in tissue response to implantation during 15, 30 and 45 days in the subcutaneous connective tissue of Wistar rats from three biomaterials: Angelus MTA®, Theracal LC®, and Angelus MTA® to which 25% bioglass G3 was added. Twenty-four Wistar rats were used, the materials were inserted into the rat’s dorsal area in silicone tubes 5 mm long by 1.5 mm diameter. Histological reaction was assessed at 15, 30, and 45 days after implantation. They were then stained with hematoxylin eosin and evaluated by two observers. Data were analyzed using Fisher’s exact test and Mann–Whitney’s U test was used to determine the association between variables. Angelus MTA induced the formation of dystrophic calcifications twice as much as Theracal LC (p < 0.05). The addition of G3 did not affect the greater or lesser occurrence of calcifications (p > 0.05). Theracal LC and MTA plus G3 caused an inflammatory reaction, which was chronic at 15 days and decreased in intensity, almost disappearing after 45 days. Theracal LC, as well as Angelus MTA plus G3, were well tolerated when implanted in the subcutaneous connective tissue of rat. Histologically, no inconvenience was found for the use by direct contact of Theracal LC, and the mixture of MTA with 25% bioactive glass G3, in the tissue of Wistar rats.

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