scholarly journals Ligninolytic enzyme production in Pleurotus eryngii depending on the medium composition and cultivation conditions

2005 ◽  
pp. 269-276
Author(s):  
Mirjana Stajic ◽  
Sonja Duletic-Lausevic ◽  
Jelena Vukojevic
Keyword(s):  
Nitrogen Concentration ◽  
Nitrogen Sources ◽  
Ligninolytic Enzyme ◽  
Medium Composition ◽  
Pleurotus Eryngii ◽  
Phenol Red ◽  
Cultivation Conditions ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Two Peaks

Pleurotus eryngii produced laccase (Lac) both under conditions of submerged fermentation (SF) and solid-state fermentation (SSF) using all of the investigated carbon and nitrogen sources, while significant peroxidases production occurred only under SSF conditions. The highest levels of Lac activity were found under SF conditions of dry ground mandarine peels (999.5 U/l). After purification of extracellular crude enzyme mixture of P. eryngii which was grown under SF conditions with dry ground mandarine peels it was revealed two peaks of Lac activity and one peak of activity against phenol red in absence of external Mn2+ which was very low (1.4 U/l). Results obtained by purification also showed that the levels of phenol red oxidation in absence of external Mn2+ were higher than phenol red oxidation levels in presence of external Mn2+. In the medium with the best carbon source for Lac production (dry ground mandarine peels), (NH4)2SO4, with a nitrogen concentration of 20 mM, was the most optimum nitrogen source among 8 investigated sources.

scholarly journals Influence of cultivation conditions on IAA - producing activity of endophytic bacterial strains isolated from morning-glory (IPOMOEA PES-CAPRAE (L.) R.Br.)

2021 ◽  
Vol 36 ◽  
pp. 05003
Author(s):  
Nguyen Van Zhang ◽  
Nguyen Thi Thu ◽  
Vu Thi Linh ◽  
V.V. Pylnev ◽  
M.I. Popchenko
Keyword(s):  
Carbon Source ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Morning Glory ◽  
Bacterial Strains ◽  
Cultivation Conditions ◽  
Bacillus Mycoides ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Study Results

This work presents the experimental study results of the influence of the culture medium on the ability to IAA synthesis of three endophytic strains TH10R, TH11T, and TH13T from roots of Ipomoea pes-caprae. Three investigated strains give the highest IAA concentration after 96 h of cultivation. A significant increase in IAA biosynthesis was obtained by cultivating the TH10R strain in a medium containing lactose or starch as a carbon source and NH4Cl or KNO3 as a nitrogen source. The TH11T strain produces the maximum amount of IAA, using glucose or xylose and KNO3 or NH4NO3 as carbon and nitrogen sources, respectively. Sucrose is a suitable carbon source for the TH13T strain; on a sucrose-containing medium, the TH13T strain produces the highest IAA amount. The most active strain is TH10R, identified as Bacillus mycoides and named Bacillus mycoides TH10R.

scholarly journals Influence of the cultivation conditions on ligninolytic enzyme production in Pleurotus pulmonarius

2007 ◽  
pp. 303-312
Author(s):  
Mirjana Stajic ◽  
Jelena Vukojevic ◽  
Sonja Duletic-Lausevic
Keyword(s):  
Carbon Source ◽  
Enzyme Production ◽  
Laccase Activity ◽  
Nitrogen Concentration ◽  
Ligninolytic Enzyme ◽  
Phenol Red ◽  
Cultivation Conditions ◽  
Pleurotus Pulmonarius ◽  
State Culture ◽  
Solid State Culture

The highest level of laccase activity (391 Ul-1), as well as significant Mn-oxidizing peroxidases production, were found in solid-state culture with grapevine sawdust as the carbon source. After purification of extracellular crude enzyme mixture of Pleurotus pulmonarius, grown in the medium with the best carbon source (grapevine sawdust), three peaks of laccase activity were noted. The results obtained by purification also showed that the levels of phenol red oxidation, in absence of external Mn2+, were higher than phenol red oxidation levels in presence of external Mn2+. The highest laccase activity was in the medium with grapevine sawdust, as carbon source, and NH4Cl at a nitrogen concentration of 30 mM (441 Ul-1). The best nitrogen source for Mn-oxidizing peroxidase production was NH4NO3 at nitrogen concentration of 30 mM. The highest laccase activity was found in the presence of 5 mM Cu2+, and 5 mM Mn2+, respectively. The absence of Cu2+ and Mn2+, as well as their presence at the concentration of 1 mM, led to the peaks of Mn-oxidizing peroxidases activities. Zn2+ and Fe2+ caused a decrease, and Se, in all investigated forms, an increase of laccase and peroxidases activities.

scholarly journals Screening for the Heterotrophic Cultivation of Chlorella sorokiniana Using an Indirect Impedance Microbiological Technique

2018 ◽  
Vol 62 (4) ◽  
Author(s):  
Balázs József Nagy ◽  
Réka Czinkóczky ◽  
Áron Németh
Keyword(s):  
Nitrogen Sources ◽  
Chlorella Sorokiniana ◽  
Added Value ◽  
Small Scale ◽  
Cultivation Conditions ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Heterotrophic Cultivation ◽  
Novel Approach ◽  
General Expectation

Heterotrophic culturing of microalgae is of great interest as it has the potential to produce feedstock for added-value bioproducts. The general expectation is to develop a method that can help screen for different media compositions and batch fermentation settings more easily and faster. In the current study, an indirect impedance microbiological technique was used to test the effects of various carbon and nitrogen sources on the growth of Chlorella sorokiniana. It was found that this technique has great potential to screen for heterotrophic cultivation conditions of microalgae as the metabolic rate of microorganisms can be determined by measuring the amount of CO2 produced. The BacTrac 4100® was proven to be a suitable instrument to compare several different small-scale culturing settings. Considering the relative changes in impedance observed, the initial cell cumber exhibits an inverse linear correlation with the detection time. Chlorella sorokiniana exhibited an enhanced degree of growth on yeast extract and tryptone, and preferred glucose over acetate or glycerol. An optimum rate of growth at a glucose concentration of 20 gL-1 was also determined. Our novel approach in the field of heterotrophic cultivation of microalgae envisages great prospects for the method in terms of the design of experiments in the field of media optimization.

scholarly journals Semi-quantitative detection for L-asparaginase producing fungi and the impact of carbon and nitrogen sources on enzyme activity

2021 ◽  
Author(s):  
Mahdi Hamed ◽  
Ahmed A Osman ◽  
Mustafa Ateş
Keyword(s):  
Enzyme Activity ◽  
Enzyme Production ◽  
Enzyme Level ◽  
Nitrogen Sources ◽  
Quantitative Detection ◽  
Phenol Red ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Fungal Isolates ◽  
The Impact

AbstractObjectiveTo semi-quantitively screen filamentous fungi isolated from different habitats for L-asparaginase production by three indicators; phenol red, cresol red and bromothymol blue and to examine the impact of different carbon and nitrogen sources on the enzyme production using different fungal isolates.Materials and methodsFifty-five fungal isolates were tested for L-asparaginase production by plate assay using Modified Czapek-Dox (MCD) medium. The enzyme activity was estimated using the Nessler method which measures the concentration of ammonia formed owing to the enzyme action on the substrate. The impact of nitrogen and carbon sources on the enzyme production was done by using the best three L-asparaginase producers from the semi-quantitative screening.Results and conclusionsA total of 53/55 (96.36%) fungal isolates were L-asparaginase producing strains, of them, Cladosporium tenuissimum, Penicillium camembertii and Aspergillus carneus showed high enzyme production. Production of L-asparaginase was higher with the glucose and urea as carbon and nitrogen sources, respectively. The highest enzyme level (5,558 U/ml) was produced by C. tenuissimum in a glucose-containing medium. This study shows that P. camemberti, A. carneus, and C. tenuissimum are good L-asparaginase producers and thus could be used for L-asparaginase production

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production
Sign in / Sign up

Export Citation Format

Share Document