Antibody Response to Newcastle Disease Virus Given by Two Different Routes as Measured by ELISA and Hemagglutination-Inhibition Test and Associated Tracheal Immunity

1985 ◽  
Vol 29 (1) ◽  
pp. 71 ◽  
Author(s):  
W. W. Marquardt ◽  
D. B. Snyder ◽  
P. K. Savage ◽  
S. K. Kadavil ◽  
F. S. Yancey
Keyword(s):  
Antibody Response ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Inhibition Test ◽  
Hemagglutination Inhibition Test

scholarly journals Commission testing of the test system “A kit for the detection of antibodies to Newcastle disease virus in hemagglutination inhibition test”

2019 ◽  
pp. 26-31
Author(s):  
B. T. Stegniy ◽  
D. V. Muzyka ◽  
S. V. Tkachenko ◽  
O. M. Rula ◽  
A. B. Stegniy ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Test System ◽  
Inhibition Test ◽  
Hemagglutination Inhibition Test ◽  
System A ◽  
Nervous System Function ◽  
Technical Specifications

Newcastle disease or pseudo-plague is a highly contagious viral bird disease, characterized by damage to the respiratory and digestive organs, as well as impaired central nervous system function. Since the middle of the last century, this disease has become widespread in many European countries. Newcastle disease is common in all continents and is especially dangerous. The article provides information on conducting a round of commission tests of the components of the test system “A Kit for Detection of Antibodies to Newcastle Disease Virus in Hemagglutination Inhibition Test”. When testing encrypted antigens and sera with previously characterized and referent samples, they were active and specific, meeting the requirements of the technical specifications of Ukraine for the specified drug. Thus, the positive antigen had activity in the hemagglutination test of 1:256, reacted only with positive to the Newcastle disease virus serum, and did not delay agglutination in the presence of referent to avian influenza virus subtypes H5 and H7 sera. In the presence of positive sera to the Newcastle disease virus in encrypted form, the positive antigen of the corresponding virus delayed the agglutination of the cock erythrocytes in dilutions 1:256–1:512. The results obtained allowed to make a positive decision on the registration of this preparation in the territory of Ukraine

scholarly journals Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry

2009 ◽  
Vol 63 (1-2) ◽  
pp. 37-44
Author(s):  
Nenad Milic ◽  
Jakov Nisavic ◽  
Marina Radojicic ◽  
Marina Sekler ◽  
Kazimir Matovic ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Blood Serum ◽  
Disease Virus ◽  
Sensitivity And Specificity ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Hemagglutination Inhibition Test ◽  
Serological Diagnostics

Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32.

scholarly journals Effect of Immunosuppression on Newcastle Disease Virus Persistence in Ducks with Different Immune Status

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Lucy W. Njagi ◽  
Phillip N. Nyaga ◽  
Lilly C. Bebora ◽  
Paul G. Mbuthia ◽  
Uswege M. Minga
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Immune Status ◽  
Experimental Period ◽  
Hemagglutination Inhibition Test ◽  
Viral Excretion ◽  
Antibody Titres ◽  
Antibody Levels ◽  
Chicken Embryo Fibroblasts

This study was carried out to verify the possibility that ducks are sources of Newcastle disease (ND) virus infection for chickens in mixed flocks. Immunosuppressed (IS) and non immunosuppressed (NIS) birds, at three different antibody levels (medium, low and absent) were used; the titres having been induced through vaccination, and Immunosuppression done using dexamethazone. Each of the 3 respective groups was further divided into 2 groups of about 12 ducks each: one challenged with velogenic ND virus; the other not challenged. Selected ducks from all groups had their antibody titres monitored serially using hemagglutination inhibition test, while two birds from each of the challenged groups were killed and respective tissues processed for ND viral recovery, using chicken embryo fibroblasts. In general, antibody titres of IS and NIS challenged ducks were significantly higher than their unchallenged counterparts (P<0.05). Non-challenged pre-immunised ducks had a progressive decrease in antibody levels; non-immunised ducks did not seroconvert. Newcastle disease virus was isolated from livers and kidneys of the challenged ducks throughout the experimental period; indicating a possibility of viral excretion, especially when the birds are stressed. It, therefore, provides another possible model of viral circulation within mixed flocks.

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