Selective inhibition of the growth of incompatible pollen tubes by S-protein in the Japanese pear

2001 ◽  
Vol 13 (4) ◽  
pp. 209-215 ◽  
Author(s):  
S. Hiratsuka ◽  
Shao-Ling Zhang ◽  
E. Nakagawa ◽  
Yoshitaka Kawai
Keyword(s):  
Selective Inhibition ◽  
Pollen Tubes ◽  
Japanese Pear ◽  
S Protein ◽  
Incompatible Pollen

scholarly journals Cultivar and Developmental Differences in S-protein Concentration and Self-incompatibility in the Japanese Pear

HortScience ◽  
2000 ◽  
Vol 35 (5) ◽  
pp. 917-920 ◽  
Author(s):  
Shao-Ling Zhang ◽  
Shin Hiratsuka
Keyword(s):  
Pollen Tubes ◽  
Tube Growth ◽  
Cultivar Differences ◽  
Japanese Pear ◽  
Pyrus Pyrifolia ◽  
Self Incompatibility ◽  
S Protein ◽  
Self Pollination ◽  
Rate Of Increase ◽  
S Proteins

Cultivars of the Japanese pear [Pyrus pyrifolia (Burm.) Nakai] have variable degrees of self-incompatibility (SI) and can be classified into at least three groups: strong, intermediate, or weak SI; as shown by the extent of self-pollen tube growth in the style, and the percentage of fruit set following self-pollination. Following self-pollination, the elongation of pollen tubes in the detached styles of `Kosui' and `Kikusui' became increasingly suppressed from 4 days before anthesis (–4 DAA) to 2 days after anthesis (2 DAA). Tube growth of `Kosui' was more suppressed than that of `Kikusui' during this period. In `Osa-Nijisseiki', however, the rate of tube growth did not vary with stage of stylar development, from –8 to 2 DAA. Pollen tubes elongated much better after cross-pollination than after self-pollination at all stages tested, and the extent of the elongation increased as the styles matured. The concentration of total S-protein (sum of two S-proteins per buffer-soluble protein) increased with stylar development, but the rate of increase varied with the cultivar. The rate was significantly greater in the strongly self-incompatible `Kosui' than in the moderately self-incompatible `Kikusui', and was slowest in the weakly self-incompatible `Osa-Nijisseiki' at all developmental stages. During stylar maturation, the concentration of S4-protein, which is common in all cultivars, was highest in `Kosui', followed by `Kikusui' and `Osa-Nijisseiki'. Thus, the cultivar differences in SI expression in the Japanese pear are determined about –4 DAA and appear to be regulated, in part, by the concentration of S-proteins produced in the style.

Ultrastructural Aspects of the Self-Incompatibility Mechanism in Lycopersicum Peruvianum Mill

1973 ◽  
Vol 12 (2) ◽  
pp. 403-419 ◽  
Author(s):  
D. DE NETTANCOURT ◽  
M. DEVREUX ◽  
A. BOZZINI ◽  
M. CRESTI ◽  
E. PACINI ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Tube Wall ◽  
Degradation Mechanism ◽  
Embryo Sac ◽  
Pollen Tubes ◽  
Self Incompatibility ◽  
Weak Reaction ◽  
General Hypothesis ◽  
Incompatible Pollen ◽  
Compatible Pollen

The experimental results obtained show that the tip of the incompatible pollen tube bursts open after the outer-wall has considerably expanded in the intercellular spaces of the conducting tissue and the inner-wall has disappeared and numerous particles have accumulated in the tube cytoplasm. These particles, which measure approximately 0.2 µm in diameter and give a weak reaction to the test of Thiéry, differ in many respects from the vesicles normally present in compatible pollen tubes growing through the style; they appear to resemble, in some cases, the spheres which are discharged by the compatible pollen tubes after they have reached the embryo-sac. It is considered that these observations support the current belief that the tube wall is the site of action for the incompatibility proteins and suggest that self-incompatibility is not a passive process resulting from lack of growth stimulation but an active event which leads to the destruction of the incompatible pollen tubes. The degradation mechanism involved appears similar to the one which enables the compatible pollen tube to release its contents in the degenerated synergid and presents some analogies with the lytic process taking place in virus-infected cells. The general hypothesis is presented that the particles observed in the cytoplasm of self-incompatible pollen tubes consist of a mixture of incompatibility proteins and of basic constituents of the tube wall.

scholarly journals The Genetical Control of Incompatibility in Phalaris Coerulescens Desf.

1956 ◽  
Vol 9 (3) ◽  
pp. 321 ◽  
Author(s):  
DL Hayman
Keyword(s):  
Day Treatment ◽  
Pollen Grains ◽  
Pollen Tubes ◽  
Multiple Alleles ◽  
Incompatible Pollen ◽  
Genetical Control

Pha/a1'is roau/c8CeJ18 Des!'. is a completely Relf-incompatible, diploid, perennial gmss. incompatible pollen gmins germinate normally but the pollen tubes fail to penetrate far into tho 8Lyle. The ineompatibility interrelationships of parents and progenios were dotermined by olmen-ing the pollen tubes and pollen grains after pollination. This eould be clone successfully only in plants induced to Hower early by eXJlosure to 100tg day treatment. Incompatibility is controlled by two loci, each with a series of multiple alleles. The loci are probably not linked but the data would not. allow detection of loose linlmge. Pollen determination is gametophytic, and the genes aet indepoudelltly in the style. 'eho rolationship of this incompatihility system to previously described systems is eonsid(lred, and theoretically possible incompatihility systems based on genetical eOlltrol nt. two loci are suggested.

Serological reactions of pollen incompatibility substances

1952 ◽  
Vol 140 (898) ◽  
pp. 127-135 ◽  
Keyword(s):  
Pollen Tube ◽  
Pollen Tubes ◽  
Cross Reactions ◽  
Antigenic Stimulus ◽  
Incompatible Pollen ◽  
S Alleles ◽  
Incompatibility Reaction

Pollen extracts of four different incompatibility genotypes S 2.4 , S 2.6 , S 3.4 , S 3.6 of Oenothera organensis were injected into rabbits to produce antisera. All the unabsorbed antisera gave precipitin reactions with all pollen extracts but the homologous extracts gave stronger reactions. Antisera which had been fully absorbed with pollen extracts heterologous in respect of S alleles gave strong reactions only with extracts which had S alleles in common with the antisera. The results of twenty-five different combinatorial tests, with the exception of two cross reactions, agree with the theory that the S alleles produce specific substances in the pollen each of which is antigenically different. Stigmas pollinated twice at a 4 hour interval with incompatible pollen showed that the pollen tubes from the second pollination were not affected by the presence of the first lot. This shows that the stylar incompatibility substance is pre-formed and is not the result of an antigenic stimulus from the pollen tubes. Pollen-tube inhibitions in the style due to the incompatibility reaction is complete and irreversible at 31°C in 30 min and after 0⋅2 to 0⋅3 mm growth.

Growth of Incompatible Pollen Tubes in Oenothera organensis

1940 ◽  
Vol 101 (4) ◽  
pp. 890-911 ◽  
Author(s):  
Sterling Emerson
Keyword(s):  
Pollen Tubes ◽  
Incompatible Pollen

scholarly journals Two Self-Incompatibility Sites Occur Simultaneously in the Same Acianthera Species (Orchidaceae, Pleurothallidinae)

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1758
Author(s):  
Mariana Oliveira Duarte ◽  
Denise Maria Trombert Oliveira ◽  
Eduardo Leite Borba
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Main Reaction ◽  
Reaction Site ◽  
Self Incompatibility ◽  
Incompatible Pollen ◽  
Plant Groups ◽  
Transmission Electron ◽  
Incompatibility System ◽  
Cytological Characteristics

In most species of Pleurothallidinae, the self-incompatibility site occurs in the stylar canal inside the column, which is typical of gametophytic self-incompatibility (GSI). However, in some species of Acianthera, incompatible pollen tubes with anomalous morphology reach the ovary, as those are obstructed in the column. We investigated if a distinct self-incompatibility (SI) system is acting on the ovary of A. johannensis, which is a species with partial self-incompatibility, contrasting with a full SI species, A. fabiobarrosii. We analyzed the morphology and development of pollen tubes in the column, ovary, and fruit using light, epifluorescence, and transmission electron microscopy. Our results show that the main reaction site in A. johannensis is in the stylar canal inside the column, which was also recorded in A. fabiobarrosii. Morphological and cytological characteristics of the pollen tubes with obstructed growth in the column indicated a process of programmed cell death in these tubes, showing a possible GSI reaction. In addition, partially self-incompatible individuals of A. johannensis exhibit a second SI site in the ovary. We suggest that this self-incompatibility site in the ovary is only an extension of GSI that acts in the column, differing from the typical late-acting self-incompatibility system recorded in other plant groups.

Pollen tube growth in tristylous Pontederia cordata (Pontederiaceae)

1986 ◽  
Vol 64 (11) ◽  
pp. 2602-2607 ◽  
Author(s):  
J. M. Anderson ◽  
S. C. H. Barrett
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Pollen Size ◽  
Tube Length ◽  
Pontederia Cordata ◽  
Incompatible Pollen ◽  
Hand Pollination ◽  
Pollen Tube Length

Pollen tube growth rates in legitimate cross- and self-pollinations were compared by controlled hand pollination in two varieties of Pontederia cordata L. (Pontederiaceae). In each pollen–stigma combination pollen germinated readily on stigmas and pollen tubes penetrated stigmatic tissue. Pollen tube length in styles was positively correlated with pollen size. In most pollinations legitimate pollen tubes grew more rapidly in styles than self pollen tubes. This difference was statistically significant in most comparisons. Inhibition of incompatible pollen tubes was either in the style or ovary. It is suggested that the mechanisms of incompatibility may differ among the floral morphs and depend on the particular pollen size involved in pollinations. The observed differences in pollen tube growth of legitimate cross- and self-pollinations in P. cordata suggest that the species is primarily outbreeding.

Identification of Residues in a Hydrophilic Loop of the Papaver rhoeas S Protein That Play a Crucial Role in Recognition of Incompatible Pollen

1998 ◽  
Vol 10 (10) ◽  
pp. 1723
Author(s):  
Katsuyuki Kakeda ◽  
Nicholas D. Jordan ◽  
Alex Conner ◽  
Jon P. Ride ◽  
Vernonica E. Franklin-Tong ◽  
...  
Keyword(s):  
Crucial Role ◽  
S Protein ◽  
Papaver Rhoeas ◽  
Incompatible Pollen ◽  
Hydrophilic Loop

scholarly journals Pollen-Stigma Interactions following Compatible and Incompatible Pollinations in Hazelnut

1993 ◽  
Vol 118 (6) ◽  
pp. 814-819 ◽  
Author(s):  
Cheryl R. Hampson ◽  
Anita Nina Azarenko ◽  
Al Soeldner
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Corylus Avellana ◽  
Pollen Tubes ◽  
Self Incompatibility ◽  
Incompatible Pollen ◽  
Tube Emergence ◽  
Stigmatic Papillae ◽  
Compatible Pollen ◽  
Scanning Electron

Scanning electron microscopy was used to describe pollen-stigma interactions during compatible and incompatible pollinations of hazelnut (Corylus avellana L.), a species possessing sporophytic self-incompatibility. The stigmatic surface is of the dry type and was covered with elongated, rounded papillae. Compatible and incompatible pollen hydrated within 2 hours of pollination. Compatible pollen tubes emerged by 4 hours and grew into the style by 12 hours after pollination. Penetration of stigmatic papillae appeared to be intracellular in some cases. In incompatible pollinations, however, pollen tube emergence was delayed until at least 8 hours. The pollen tubes were distorted and did not penetrate the stigma.

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