scholarly journals Expressions of alkaline phosphatase genes during phosphate starvation are under positive influences of multiple cell wall hydrolase genes in Bacillus subtilis

2016 ◽  
Vol 62 (2) ◽  
pp. 106-109 ◽  
Author(s):  
Yueh-Chi Shen ◽  
Yi-Nei Hu ◽  
Gwo-Chyuan Shaw
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Wall ◽  
Bacillus Subtilis ◽  
Phosphate Starvation ◽  
Cell Wall Hydrolase ◽  
Multiple Cell

scholarly journals Analysis of Bacillus subtilis tagAB andtagDEF Expression during Phosphate Starvation Identifies a Repressor Role for PhoP∼P

1998 ◽  
Vol 180 (3) ◽  
pp. 753-758 ◽  
Author(s):  
Wei Liu ◽  
Stephen Eder ◽  
F. Marion Hulett
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Teichoic Acid ◽  
Phosphate Starvation ◽  
Acid Synthesis ◽  
Pho Regulon ◽  
Wall Teichoic Acid ◽  
Negative Role ◽  
First Time ◽  
Starvation Conditions

ABSTRACT The tagAB and tagDEF operons, which are adjacent and divergently transcribed, encode genes responsible for cell wall teichoic acid synthesis in Bacillus subtilis. TheBacillus data presented here suggest that PhoP and PhoR are required for direct repression of transcription of the two operons under phosphate starvation conditions but have no regulatory role under phosphate-replete conditions. These data identify for the first time that PhoP∼P has a negative role in Pho regulon gene regulation.

scholarly journals Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis. .

1995 ◽  
Vol 177 (19) ◽  
pp. 5582-5589 ◽  
Author(s):  
J Sekiguchi ◽  
K Akeo ◽  
H Yamamoto ◽  
F K Khasanov ◽  
J C Alonso ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Nucleotide Sequence ◽  
Cell Wall Hydrolase

scholarly journals Bacillus subtilis transcription regulator, Spo0A, decreases alkaline phosphatase levels induced by phosphate starvation.

1993 ◽  
Vol 175 (12) ◽  
pp. 3749-3756 ◽  
Author(s):  
K K Jensen ◽  
E Sharkova ◽  
M F Duggan ◽  
Y Qi ◽  
A Koide ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bacillus Subtilis ◽  
Phosphate Starvation ◽  
Transcription Regulator

scholarly journals SweC and SweD are essential co-factors of the FtsEX-CwlO cell wall hydrolase complex in Bacillus subtilis

PLoS Genetics ◽  
2019 ◽  
Vol 15 (8) ◽  
pp. e1008296 ◽  
Author(s):  
Yannick R. Brunet ◽  
Xindan Wang ◽  
David Z. Rudner
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Cell Wall Hydrolase

scholarly journals The lytE Gene of Bacillus subtilis 168 Encodes a Cell Wall Hydrolase

1998 ◽  
Vol 180 (8) ◽  
pp. 2272-2272
Author(s):  
Philippe Margot ◽  
Michael Whalen ◽  
Ahmad Gholamhoseinian ◽  
Patrick Piggot ◽  
Dimitri Karamata
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Bacillus Subtilis 168 ◽  
Cell Wall Hydrolase ◽  
A Cell

scholarly journals Regulation of a New Cell Wall Hydrolase Gene,cwlF, Which Affects Cell Separation in Bacillus subtilis

1998 ◽  
Vol 180 (9) ◽  
pp. 2549-2555 ◽  
Author(s):  
Shu Ishikawa ◽  
Yoshiko Hara ◽  
Ryo Ohnishi ◽  
Junichi Sekiguchi
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Sequence Similarity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Exponential Growth Phase ◽  
High Sequence Similarity ◽  
Cell Wall Hydrolase ◽  
Mutant Cells

ABSTRACT Bacillus subtilis produces a 35-kDa cell wall hydrolase, CwlF, during vegetative growth. The CwlF protein was extracted from B. subtilis cwlB sigD mutant cells and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-terminal amino acid sequencing revealed that its sequence is completely identical to that of the internal region of thepapQ gene product. Disruption of the papQ gene in the B. subtilis chromosome led to the complete loss of CwlF, indicating that papQ is identical tocwlF. CwlF exhibits high sequence similarity to the p60 proteins of Listeria species, NlpC proteins ofEscherichia coli and Haemophilus influenzae, and Enp2 protein of Bacillus sphaericus. The β-galactosidase activity of the cwlF-lacZ transcriptional fusion and Northern blot analysis of the cwlF gene indicated that the gene is expressed as a monocistronic operon during the exponential growth phase, and primer extension analysis suggested that the cwlF gene is transcribed mainly by EςA RNA polymerase and weakly by EςH RNA polymerase. While the cells of the cwlF-deficient mutant were about twice as long as those of the wild-type strain, the cwlF sigD double mutant cells exhibited extraordinary microfiber formation, in contrast to the filamentation of the sigD mutant. The CwlF production was not affected by the pleiotropic mutationsflaD1 and degU32(Hy), which endow cells with the ability of extensive filamentation.

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