The First Reported Case of Microsporum canis Infection in Kyushu District

Latar Belakang: Dermatofitosis adalah suatu kondisi penyakit yang ditandai dengan infeksi pada jaringan berkeratin seperti epidermis, rambut dan kuku. Kondisi ini disebabkan oleh sekelompok jamur berfilamen terkait yang dikenal sebagai dermatofita. Bawang dayak (Eleutherine americana Merr.) merupakan tanaman berumbi merah yang mengandung senyawa bioaktif yang memiliki kemampuan menghambat pertumbuhan jamur golongan dermatofita. Metode: Umbi bawang dayak diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Uji aktivitas antijamur menggunakan metode difusi cakram Kirby-Bauer dengan 5 variasi konsentrasi yaitu 60%, 30%, 15%, 7,5% dan 3,75%. Kontrol positif yang digunakan adalah itrakonazol 8 µg/disk sedangkan kontrol negatif yang digunakan adalah pelarut Tween 80 sebesar 10%. Hasil: Ekstrak umbi bawang dayak mengandung senyawa metabolit sekunder berupa saponin, kuinon, flavonoid, fenol, tanin, alkaloid, steroid dan triterpenoid. Uji aktivitas antijamur ekstrak etanol umbi bawang dayak dengan metode difusi cakram tidak membentuk zona hambat terhadap pertumbuhan Microsporum canis. Kesimpulan: Ekstrak etanol umbi bawang dayak tidak memiliki aktivitas antijamur terhadap pertumbuhan Microsporum canis.

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Faculty Opinions recommendation of Population structure and evolutionary origins of Microsporum canis, M. ferrugineum and M. audouinii.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1087061.540030 ◽

2007 ◽

Author(s):

Joseph Heitman

Keyword(s):

Population Structure ◽

Microsporum Canis ◽

Evolutionary Origins

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Tinea corporis bullosa due to Microsporum canis mimicking linear IgA bullous dermatosis

European Journal of Dermatology ◽

10.1684/ejd.2012.1837 ◽

2012 ◽

Vol 22 (6) ◽

pp. 805-806 ◽

Cited By ~ 2

Author(s):

A. Susanne Aalfs ◽

Marcel F. Jonkman

Keyword(s):

Tinea Corporis ◽

Microsporum Canis ◽

Bullous Dermatosis ◽

Linear Iga Bullous Dermatosis

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A Fungal Defensin Targets the SARS−CoV−2 Spike Receptor−Binding Domain

Journal of Fungi ◽

10.3390/jof7070553 ◽

2021 ◽

Vol 7 (7) ◽

pp. 553

Author(s):

Bin Gao ◽

Shunyi Zhu

Keyword(s):

Receptor Binding ◽

Viral Entry ◽

Single Point ◽

Binding Domain ◽

Host Cells ◽

Single Point Mutation ◽

Binding Motif ◽

Microsporum Canis ◽

Receptor Binding Domain ◽

Fungal Defensin

Coronavirus Disease 2019 (COVID−19) elicited by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS−CoV−2) is calling for novel targeted drugs. Since the viral entry into host cells depends on specific interactions between the receptor−binding domain (RBD) of the viral Spike protein and the membrane−bound monocarboxypeptidase angiotensin converting enzyme 2 (ACE2), the development of high affinity RBD binders to compete with human ACE2 represents a promising strategy for the design of therapeutics to prevent viral entry. Here, we report the discovery of such a binder and its improvement via a combination of computational and experimental approaches. The binder micasin, a known fungal defensin from the dermatophytic fungus Microsporum canis with antibacterial activity, can dock to the crevice formed by the receptor−binding motif (RBM) of RBD via an extensive shape complementarity interface (855.9 Å2 in area) with numerous hydrophobic and hydrogen−bonding interactions. Using microscale thermophoresis (MST) technique, we confirmed that micasin and its C−terminal γ−core derivative with multiple predicted interacting residues exhibited a low micromolar affinity to RBD. Expanding the interface area of micasin through a single point mutation to 970.5 Å2 accompanying an enhanced hydrogen bond network significantly improved its binding affinity by six−fold. Our work highlights the naturally occurring fungal defensins as an emerging resource that may be suitable for the development into antiviral agents for COVID−19.

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