Therapeutic Effects of Olopatadine Hydrochloride on Blood Substance P Levels and Circulating Th17 Cell Numbers in Patients with Atopic Dermatitis

The extracts of Schisandra chinensis (Turcz.) Baill. (Schisandraceae) have various therapeutic effects, including inflammation and allergy. In this study, gomisin M2 (GM2) was isolated from S. chinensis and its beneficial effects were assessed against atopic dermatitis (AD). We evaluated the therapeutic effects of GM2 on 2,4-dinitrochlorobenzene (DNCB) and Dermatophagoides farinae extract (DFE)-induced AD-like skin lesions with BALB/c mice ears and within the tumor necrosis factor (TNF)-α and interferon (IFN)-γ-stimulated keratinocytes. The oral administration of GM2 resulted in reduced epidermal and dermal thickness, infiltration of tissue eosinophils, mast cells, and helper T cells in AD-like lesions. GM2 suppressed the expression of IL-1β, IL-4, IL-5, IL-6, IL-12a, and TSLP in ear tissue and the expression of IFN-γ, IL-4, and IL-17A in auricular lymph nodes. GM2 also inhibited STAT1 and NF-κB phosphorylation in DNCB/DFE-induced AD-like lesions. The oral administration of GM2 reduced levels of IgE (DFE-specific and total) and IgG2a in the mice sera, as well as protein levels of IL-4, IL-6, and TSLP in ear tissues. In TNF-α/IFN-γ-stimulated keratinocytes, GM2 significantly inhibited IL-1β, IL-6, CXCL8, and CCL22 through the suppression of STAT1 phosphorylation and the nuclear translocation of NF-κB. Taken together, these results indicate that GM2 is a biologically active compound that exhibits inhibitory effects on skin inflammation and suggests that GM2 might serve as a remedy in inflammatory skin diseases, specifically on AD.

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Nerve growth factor and substance P are useful plasma markers of disease activity in atopic dermatitis

British Journal of Dermatology ◽

10.1046/j.1365-2133.2002.04803.x ◽

2002 ◽

Vol 147 (1) ◽

pp. 71-79 ◽

Cited By ~ 217

Author(s):

M. Toyoda ◽

M. Nakamura ◽

T. Makino ◽

T. Hino ◽

M. Kagoura ◽

...

Keyword(s):

Atopic Dermatitis ◽

Nerve Growth Factor ◽

Growth Factor ◽

Substance P ◽

Disease Activity ◽

Nerve Growth ◽

Plasma Markers

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Crocin ameliorated skin tissue inflammation in atopic dermatitis in mice

International Journal of Research in Dermatology ◽

10.18203/issn.2455-4529.intjresdermatol20200592 ◽

2020 ◽

Vol 6 (2) ◽

pp. 142

Author(s):

Abdullah Alyoussef

Keyword(s):

Atopic Dermatitis ◽

Protein Expression ◽

Skin Disease ◽

Therapeutic Effects ◽

Inflammatory Skin Disease ◽

Mice Model ◽

Subsequent Reduction ◽

Gardenia Jasminoides ◽

Tnf Α ◽

Gene And Protein Expression

Background: Atopic dermatitis (AD) is considered a chronic recurrent inflammatory skin disease. In addition, crocin is the major carotenoid compound found in Gardenia jasminoides. It is previously proved to produce anti-inflammatory actions. Therefore, we conducted this research to investigate the therapeutic effects of crocin on a mice model of AD.Methods: Mice were investigated for the number of scratches and dermatitis score. Skin was isolated and used for measurements of gene and protein expression of β-catenin, NFκB, TNF-α and IL-1β.Results: Authors found that crocin significantly reduced the number of scratches, ear thickness and dermatitis score. In addition, crocin ameliorated AD-induced elevation in the expression of β-catenin, NFκB, TNF-α and IL-1β.Conclusions: Crocin ameliorated DNCB-induced AD in mice via blockage of β-catenin with subsequent reduction in inflammatory pathway.

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