139-OR: GIP Regulates Fatty Acid and Glycerol Synthesis in Human Adipocytes

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 139-OR
Author(s):  
PAUL L. MILLIGAN ◽  
KENNETH D. ROTH ◽  
HAITAO HU ◽  
JULIE MOYERS ◽  
XIAOPING RUAN ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Human Adipocytes ◽  
Glycerol Synthesis

Calcium-dependent release of adipocyte fatty acid binding protein from human adipocytes

2014 ◽  
Vol 122 (03) ◽  
Author(s):  
I Schlottmann ◽  
M Ehrhart-Bornstein ◽  
M Wabitsch ◽  
SR Bornstein ◽  
V Lamounier-Zepter
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Human Adipocytes ◽  
Fatty Acid Binding ◽  
Calcium Dependent ◽  
Acid Binding Protein

scholarly journals A novel JNK2/SREBP-1c pathway involved in insulin-induced fatty acid synthesis in human adipocytes

2013 ◽  
Vol 54 (6) ◽  
pp. 1531-1540 ◽  
Author(s):  
Minoru Ito ◽  
Michiaki Nagasawa ◽  
Naoki Omae ◽  
Masaki Tsunoda ◽  
Junichi Ishiyama ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Human Adipocytes

scholarly journals Determining contributions of exogenous glucose and fructose to de novo fatty acid and glycerol synthesis in liver and adipose tissue

2019 ◽  
Vol 56 ◽  
pp. 69-76 ◽  
Author(s):  
João C.P. Silva ◽  
Cátia Marques ◽  
Fátima O. Martins ◽  
Ivan Viegas ◽  
Ludgero Tavares ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
De Novo ◽  
Exogenous Glucose ◽  
Glycerol Synthesis

Fatty acid synthase and in vitro adipogenic response of human adipocytes inhibited by α and α′ subunits of soybean β-conglycinin hydrolysates

2010 ◽  
Vol 119 (4) ◽  
pp. 1571-1577 ◽  
Author(s):  
E. Gonzalez de Mejia ◽  
C. Martinez-Villaluenga ◽  
M. Roman ◽  
N.A. Bringe
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Human Adipocytes ◽  
Α Subunits

Direct Free Fatty Acid Uptake Into Human Adipocytes In Vivo: Relation to Body Fat Distribution

Diabetes ◽  
2007 ◽  
Vol 56 (5) ◽  
pp. 1369-1375 ◽  
Author(s):  
S. Shadid ◽  
C. Koutsari ◽  
M. D. Jensen
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Body Fat ◽  
Fat Distribution ◽  
Fatty Acid Uptake ◽  
Body Fat Distribution ◽  
Acid Uptake ◽  
Human Adipocytes

scholarly journals Adiponectin Inhibits Spontaneous and Catecholamine-Induced Lipolysis in Human Adipocytes of Non-Obese Subjects Through AMPK-Dependent Mechanisms

2011 ◽  
pp. 139-148 ◽  
Author(s):  
Z. WEDELLOVÁ ◽  
J. DIETRICH ◽  
M. ŠIKLOVÁ-VÍTKOVÁ ◽  
K. KOLOŠTOVÁ ◽  
M. KOVÁČIKOVÁ ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Acid Metabolism ◽  
Adenosine Monophosphate ◽  
Inhibitory Effect ◽  
Glycerol Concentration ◽  
Human Adipocytes ◽  
Compound C ◽  
Obese Subjects ◽  
Surgical Operations

Adiponectin is an adipokine increasing glucose and fatty acid metabolism and improving insulin sensitivity. The aim of this study was to investigate the role of adiponectin in the regulation of adipocyte lipolysis. Human adipocytes isolated from biopsies obtained during surgical operations from 16 non-obese and 17 obese subjects were incubated with 1) human adiponectin (20 µg/ml) or 2) 0.5 mM AICAR – activator of AMPK (adenosine monophosphate activated protein kinase). Following these incubations, isoprenaline was added (10-6 M) to investigate the influence of adiponectin and AICAR on catecholamine-induced lipolysis. Glycerol concentration was measured as lipolysis marker. We observed that adiponectin suppressed spontaneous lipolysis by 21 % and isoprenaline-induced lipolysis by 14 % in non-obese subjects. These effects were not detectable in obese individuals, but statistically significant differences in the effect of adiponectin between obese and non-obese were not revealed by two way ANOVA test. The inhibitory effect of AICAR and adiponectin on lipolysis was reversed by Compound C. Our results suggest, that adiponectin in physiological concentrations inhibits spontaneous as well as catecholamine-induced lipolysis. This effect might be lower in obese individuals and this regulation seems to involve AMPK.

scholarly journals Mining microarray datasets in nutrition: expression of the GPR120 (n-3 fatty acid receptor/sensor) gene is down-regulated in human adipocytes by macrophage secretions

2012 ◽  
Vol 1 ◽  
Author(s):  
Paul Trayhurn ◽  
Gareth Denyer
Keyword(s):  
Fatty Acid ◽  
Conditioned Medium ◽  
Mrna Level ◽  
Bioinformatic Analysis ◽  
Human Adipocytes ◽  
Acid Receptor ◽  
Microarray Datasets ◽  
G Protein Coupled ◽  
Source Of Information ◽  
Fatty Acid Receptor

AbstractMicroarray datasets are a rich source of information in nutritional investigation. Targeted mining of microarray data following initial, non-biased bioinformatic analysis can provide key insight into specific genes and metabolic processes of interest. Microarrays from human adipocytes were examined to explore the effects of macrophage secretions on the expression of the G-protein-coupled receptor (GPR) genes that encode fatty acid receptors/sensors. Exposure of the adipocytes to macrophage-conditioned medium for 4 or 24 h had no effect on GPR40 and GPR43 expression, but there was a marked stimulation of GPR84 expression (receptor for medium-chain fatty acids), the mRNA level increasing 13·5-fold at 24 h relative to unconditioned medium. Importantly, expression of GPR120, which encodes an n-3 PUFA receptor/sensor, was strongly inhibited by the conditioned medium (15-fold decrease in mRNA at 24 h). Macrophage secretions have major effects on the expression of fatty acid receptor/sensor genes in human adipocytes, which may lead to an augmentation of the inflammatory response in adipose tissue in obesity.

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