scholarly journals Antibacterial and antibiofilm effect of silver and gold nanoparticles in Uropathogenic Escherichia coli

2021 ◽  
Vol 27 (2) ◽  
pp. 67-72
Author(s):  
Rini Purbowati ◽  
Sugiharto Sugiharto ◽  
Agusniar Furkani Listyawati ◽  
Masfufatun Masfufatun ◽  
Lusiani Tjandra ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Nosocomial Infections ◽  
Infectious Agent ◽  
Microtiter Plate ◽  
Uropathogenic Escherichia Coli ◽  
Antibiofilm Activity ◽  
Infectious Agents ◽  
Silver And Gold Nanoparticles ◽  
Life Threatening

Nosocomial infections of the urinary tract are the most common nosocomial infections. Infectious diseases currently pose a life threatening due to the increasing phenomenon of infectious agent resistance. One of the factors known to cause resistance to broad-spectrum antimicrobials agent is the susceptibility to biofilm formation. The use of silver nanoparticles (NPs) is now regarded as one of the most promising strategies to target infections associated with biofilms. This study aims to determine the application of nanotechnology in the form of metal nanoparticles in targeting the formation of biofilms on infectious agents. The antibacterial and antibiofilm activity test was performed on a 96-well microtiter plate on uropathogenic Escherichia coli (UPEC) at concentrations of 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 ppm. The results showed the addition of silver and gold nanoparticles at different concentrations affected and inhibited the growth of UPEC and prevented the formation of biofilms, as indicated by a decrease in the OD value. In conclusion, silver and gold nanoparticles have antibacterial and antibiofilm effects on UPEC.

scholarly journals Antibiofilm activity of trans-cinnamaldehyde, p-coumaric, and ferulic acids on uropathogenic Escherichia coli

2015 ◽  
Vol 45 ◽  
pp. 919-924 ◽  
Author(s):  
Barbara KOT ◽  
Jolanta WICHA ◽  
Małgorzata PIECHOTA ◽  
Katarzyna WOLSKA ◽  
Agata GRUŻEWSKA
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli ◽  
Antibiofilm Activity ◽  
Ferulic Acids

scholarly journals Antibacterial Potency of Black Garlic Extract from Allium sativum on Escherichia coli

2021 ◽  
Vol 3 (3) ◽  
pp. 124-131
Author(s):  
Muhammad Alief Harun ◽  
Sultan Buraena ◽  
Eny Arlini Wello ◽  
Hasta Handayani Idrus ◽  
Andi Sitti Fahirah Arsal
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Allium Sativum ◽  
Infectious Agent ◽  
Garlic Extract ◽  
Infectious Agents ◽  
Spontaneous Fermentation ◽  
Global Issues ◽  
Good Antibacterial Activity ◽  
Fermented Product

Background: Diarrhea is still one of the global issues especially in developing countries. Diarrhea can be caused by either an infectious agent or a non-infectious agent. Escherichia coli is one of the infectious agents that is responsible for causing diarrhea. Garlic (Allium sativum) is a plant that has a good antibacterial activity potential because of its organosulfur and phenolic compound. Black garlic is the product of spontaneous fermentation of garlic that has improved its bioactive compounds. Content: Antibacterial potency of black garlic extract on Escherichia coli is shown on the resistance zone that formed where the lowest zone diameter is 9,67 mm while the highest zone is 24 mm. The fermentation of black garlic happened at the temperature of 70-80°C with 75-80% for 28-40 days. Conclusion: Black garlic is shown to exhibit antibacterial activity against bacteria such as Escherichia coli. This fermented product has the potential to be a therapeutic agent for diseases caused by Escherichia coli.

scholarly journals The Antibiofilm Efficacy of Gold Nanoparticles Against Acinetobacter baumannii

2020 ◽  
pp. 749-753
Author(s):  
Mahmood Jasim Mohammed Al-Shaabani ◽  
Ahmed Mohammed Turki ◽  
Harith J. Fahad Al-Mathkhury
Keyword(s):  
Gold Nanoparticles ◽  
Acinetobacter Baumannii ◽  
Microtiter Plate ◽  
Clinical Isolates ◽  
Antibiofilm Activity ◽  
Chronic Infections ◽  
Plate Method ◽  
Hospital Environments

Acinetobacter baumannii is highly adapted to hospital environments, causing persistent chronic infections due to its ability to form biofilms. In this work, the antibiofilm activity of AuNPs with a subMIC concentration of 9.34 μg/ml was investigated by the microtiter plate method against 80 clinical isolates of A. baumannii. The results revealed that the biofilm was significantly (P< 0.05) reduced by 48.2 – 82.1%.

scholarly journals Synthesis and Characterization of Silver and Gold Nanoparticles Using Aqueous Extract of Seaweed,Turbinaria conoides,and Their Antimicrofouling Activity

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Sri Ramkumar Vijayan ◽  
Prakash Santhiyagu ◽  
Muthukkumarasamy Singamuthu ◽  
Natarajan Kumari Ahila ◽  
Ravindran Jayaraman ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Aqueous Extract ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Antibiofilm Activity ◽  
Ag Nps ◽  
Au Nps ◽  
Silver And Gold Nanoparticles ◽  
Marine Biofilm ◽  
Turbinaria Conoides

Silver and gold nanoparticles were synthesized using an aqueous extract of the seaweedTurbinaria conoidesand their antibiofilm activity against marine biofilm forming bacteria is reported here. The UV-Vis spectra showed the characteristics SPR absorption band for Ag NPs at 421 and for Au NPs at 538 nm. Further, the synthesized nanoparticles were characterized using FT-IR, XRD, FESEM, EDX, and HRTEM analysis. Spherical and triangular nanostructures of the Ag and Au nanoparticles were observed between the size ranges of 2–17 nm and 2–19 nm, respectively. The synthesized Ag NPs are efficient in controlling the bacterial biofilm formation; however, Au NPs did not show any remarkable antibiofilm activity. The maximum zone of inhibition was recorded againstE. coli(17.6±0.42 mm), followed bySalmonellasp.,S. liquefaciens, andA. hydrophila. The macrotube dilution method inferred the MIC (20–40 µL mL−1) and MBC (40–60 µL mL−1) of Ag NPs. The CLSM images clearly showed the weak adherence and disintegrating biofilm formation of marine biofilm bacterial strains treated with Ag NPs. TheArtemiacytotoxicity assay recorded the LC50value of88.914±5.04 µL mL−1. Thus the present study proved the efficiency of Ag NPs as a potent antimicrofouling agent and became the future perspective for the possible usage in the biofouling related issues in the aquaculture installations and other marine systems.

64: Triclosan Decreases Uropathogenic Escherichia Coli Adherence to Bladder Epithelial Cells

2006 ◽  
Vol 175 (4S) ◽  
pp. 22-22
Author(s):  
Chelsea N. Elwood ◽  
Shannon Mifflin ◽  
Peter A. Cadieux ◽  
Ben H. Chew ◽  
Gregor Reid ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Uropathogenic Escherichia Coli
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