scholarly journals EFEKTIVITAS EKSTRAK PROPOLIS DALAM PENYEMBUHAN LUKA BAKAR DERAJAT II PADA HEWAN UJI

2021 ◽  
Vol 1 (2) ◽  
pp. 80
Author(s):  
Komang Sherly Ulandari ◽  
Putu Cahyani Paramita Yoga ◽  
Made Widya Lestari ◽  
Ni Nyoman Mestri Agustini
Keyword(s):  
Reactive Oxygen Species ◽  
Ferulic Acid ◽  
Tissue Repair ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Literature Study ◽  
Propolis Extract ◽  
Average Value ◽  
Different Doses ◽  
Second Degree

Luka bakar merupakan salah satu insiden yang sering terjadi dalam kehidupan sehari-hari. Tujuan dari penelitian berdasarkan studi pustaka ini adalah untuk mengetahui efektivitas ekstrak propolis dalam penyembuhan luka bakar derajat II pada hewan uji. Propolis kaya akan manfaat seperti menjadi antimikroba, antibakteri, antimikotik, astringent, spasmolitik, anti-inflamasi, anestesi, antioksidan, antiulcer, antikanker, serta memiliki efek imunomodulator yang dapat mendukung penyembuhan luka bakar derajat II. Senyawa flavonoid, fenol, arginine, asam ferulat, dan albumin berperan baik dalam menurunkan pertumbuhan mikroorganisme, mempercepat perbaikan jaringan dan menghambat munculnya ROS atau Reactive Oxygen Species pada luka bakar.Ekstrak propolis dengan konsentrasi 100% telah terbukti lebih efektif dalam mempercepat penyembuhan luka bakar derajat II pada hewan uji. Pemberian propolis dengan dosis pemberian yang berbeda menunjukkan perbedaan lamanya waktu penyembuhan luka bakar yang ditandai oleh perbedaan nilai rata-rata luas luka bakar dalam setiapwaktu pengukuran. Berdasarkan analisis hipotesis peneliti, efektivitas penyembuhan luka bakar dipengaruhi oleh dosis pemberian ekstrak propolis pada luka bakar tersebut. Dimana keduanya memiliki hubungan yang sebanding, peningkatan dosis pemberian propolis dapat mempercepat penyembuhan luka bakar derajat II.Burns are one of the incidents that often occur in life. The purpose of this research based on literature study was to determine the effectiveness of propolis extract in healing second degree burns in tested animals. Propolis is rich in benefits such as being antimicrobial, antibacterial, antimycotic, astringent, spasmolytic, anti- inflammatory, anesthetic, antioxidant, antiulcer, anticancer, and has an immunomodulatory effect that can support healing of second degree burns. Flavonoids, phenols, arginine, ferulic acid, and albumin have a good role in reducing the growth of microorganisms, accelerating tissue repair and inhibiting the appearance of ROS or Reactive Oxygen Species in burns.Propolis extract with a concentration of 100% has been proven to be more effective in accelerating the healing of second degree burns in test animals. Giving propolis with different doses showed a difference in length of time to heal burns, indicated by differences in the average value of burns in each measurement time. Based on the analysis of the researchers' hypothesis, the effectiveness of healing burns was influenced by the dose of propolis extract on the burns. Both have an equal relation, increasing thedose of giving propolis can accelerate the healing of second degree burns Keywords: second degree burns, propolis, effectiveness of propolisextrac

scholarly journals Vesicular Formation of Trans-Ferulic Acid: an Efficient Approach to Improve the Radical Scavenging and Antimicrobial Properties

2021 ◽  
Author(s):  
Anahita Rezaeiroshan ◽  
Majid Saeedi ◽  
Katayoun Morteza-Semnani ◽  
Jafar Akbari ◽  
Akbar Hedayatizadeh-Omran ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Reactive Oxygen Species ◽  
Ferulic Acid ◽  
Reactive Oxygen Species Production ◽  
Radical Scavenging ◽  
Reactive Oxygen ◽  
Entrapment Efficiency ◽  
The Body ◽  
Oxygen Species ◽  
Species Production

Abstract Purposes Reactive oxygen species production is harmful to human’s health. The presence of antioxidants in the body may help to diminish reactive oxygen species. Trans-ferulic acid is a good antioxidant, but its low water solubility excludes its utilization. The study aims to explore whether a vesicular drug delivery could be a way to overcome the poor absorption of trans-ferulic acid hence improving its antimicrobial efficiency and antioxidant effect. Methods Niosomal vesicles containing the drug were prepared by film hydration method. The obtained vesicles were investigated in terms of morphology, size, entrapment efficiency, release behavior, cellular cytotoxicity, antioxidant, cellular protection study, and antimicrobial evaluations. Results The optimized niosomal formulation had a particle size of 158.7 nm and entrapment efficiency of 21.64%. The results showed that the optimized formulation containing 25 μM of trans-ferulic acid could enhance the viability of human foreskin fibroblast HFF cell line against reactive oxygen species production. The minimum effective dose of the plain drug and the niosomal formulation against Staphylococcus aurous (ATCC 29213) was 750 µg/mL and 375 µg/mL, respectively, and for Escherichia coli (ATCC 25922), it was 750 µg/mL and 187/5 µg/mL, respectively. The formulation could also improve the minimum bactericidal concentration of the drug in Staphylococcus aurous, Escherichia coli, and Acinobacter baumannii (ATCC 19606). Conclusion These results revealed an improvement in both antibacterial and antioxidant effects of the drug in the niosomal formulation.

Reactive Oxygen Species (ROS)-Responsive Nanoprobe For Bioimaging And Targeted Therapy of Osteoarthritis

2021 ◽  
Author(s):  
Chong Shen ◽  
Ming Gao ◽  
Haimin Chen ◽  
Yanting Zhan ◽  
Qiumei Lan ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cartilage Damage ◽  
Reactive Oxygen ◽  
Disease Classification ◽  
Oxygen Species ◽  
Stimulus Responsive ◽  
Targeting Peptide ◽  
Physiological Markers ◽  
Different Doses ◽  
Selection Of

Abstract Stimulus-responsive therapy that allows precise imaging-guided therapy is limited for osteoarthritis (OA) therapy due to the selection of proper physiological markers as stimulus. Based on that the over-production of Reactive Oxygen Species (ROS) is one of the leading causes of OA, we selected ROS as markers and designed a cartilage-targeting and ROS-responsive theranostic nanoprobe that is highly specific for effective bioimaging and therapy of OA. This nanoprobe was fabricated by using PEG micelles modified with ROS-sensitive thioketal linkers (TK) and cartilage-targeting peptide, termed TKC, which was then encapsulated with Dexamethasone (DEX) to form TKC@DEX nanoparticles. Results showed that the nanoprobe can smartly “turn on” in response to excessive ROS and “turn off” in the normal joint. By applying different doses of ROS inducer and ROS inhibitor, this nanoprobe can emit ROS-dependent fluorescence according to the degree of OA severity, helpful to precise disease classification in clinic. Specifically targeting cartilage, TKC@DEX could effectively respond to ROS and sustained release DEX to remarkably reduce cartilage damage in the OA joints. This smart, sensitive and endogenously activated ROS-responsive nanoprobe is promising for OA theranostics.

scholarly journals GENERATION OF REACTIVE OXYGEN SPECIES IN RAT EPIDIDYMAL SPERMATOZOA AFTER CYCLOPHOSPHAMIDE TREATMENT

2018 ◽  
Vol 11 (4) ◽  
pp. 437
Author(s):  
Kashmiri Zn ◽  
Sastry Ms
Keyword(s):  
Reactive Oxygen Species ◽  
Sperm Morphology ◽  
Reactive Oxygen ◽  
Wistar Rat ◽  
Sperm Function ◽  
Oxygen Species ◽  
Cyclophosphamide Treatment ◽  
Epididymal Spermatozoa ◽  
Vehicle Treated Control ◽  
Different Doses

 Objective: The aim of this study was to examine the effect of an anticancer drug cyclophosphamide (CPA) on generation of reactive oxygen species (ROS) in epididymal spermatozoa of Wistar rat, Rattus norvegicus. Methods: For this purpose, the rats were injected intraperitoneally with different doses of CPA (5 mg, 15 mg, and 20 mg/Kg BW for 2 weeks).Results: Treatment resulted into significantly increased level of ROS in CPA-treated groups when compared to the vehicle-treated control group.Conclusion: The results revealed that CPA has deleterious effect on the sperm morphology and physiology, which is dose and duration dependent and at certain doses cause the production of a number of reactive molecules and free radicals derived from molecular oxygen consequently resulting into adverse effect on the sperm function and hence on reproduction.

scholarly journals Reactive oxygen species (ROS)-responsive nanoprobe for bioimaging and targeting therapy of osteoarthritis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Chong Shen ◽  
Ming Gao ◽  
Haimin Chen ◽  
Yanting Zhan ◽  
Qiumei Lan ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cartilage Damage ◽  
Reactive Oxygen ◽  
Disease Classification ◽  
Oxygen Species ◽  
Targeting Therapy ◽  
Targeting Peptide ◽  
Physiological Markers ◽  
Different Doses ◽  
Selection Of

AbstractStimulus-responsive therapy that allows precise imaging-guided therapy is limited for osteoarthritis (OA) therapy due to the selection of proper physiological markers as stimulus. Based on that the over-production of Reactive Oxygen Species (ROS) is associated with the progression in OA, we selected ROS as markers and designed a cartilage targeting and ROS-responsive theranostic nanoprobe that can be used for effective bioimaging and therapy of OA. This nanoprobe was fabricated by using PEG micelles modified with ROS-sensitive thioketal linkers (TK) and cartilage-targeting peptide, termed TKCP, which was then encapsulated with Dexamethasone (DEX) to form TKCP@DEX nanoparticles. Results showed that the nanoprobe can smartly “turn on” in response to excessive ROS and “turn off” in the normal joint. By applying different doses of ROS inducer and ROS inhibitor, this nanoprobe can emit ROS-dependent fluorescence according to the degree of OA severity, helpful to precise disease classification in clinic. Specifically targeting cartilage, TKCP@DEX could effectively respond to ROS and sustained release DEX to remarkably reduce cartilage damage in the OA joints. This smart, sensitive and endogenously activated ROS-responsive nanoprobe is promising for OA theranostics. Graphical Abstract

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