In Vitro Quality Evaluation of Amoxicillin Trihydrate Capsules Marketed in Gaza Strip-Palestine

Eugenyl-β-D-glucopyranoside, also referred to as Citrusin C, is a natural glucoside found among others in cloves, basil and cinnamon plants. Eugenol in a form of free aglycone is used in perfumeries, flavourings, essential oils and in medicinal products. Synthetic Citrusin C was incubated with human saliva in several in vitro models together with substrate-specific enzyme and antibiotics (clindamycin, ciprofloxacin, amoxicillin trihydrate and potassium clavulanate). Citrusin C was detected using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Citrusin C was completely degraded only when incubated with substrate-specific A. niger glucosidase E.C 3.2.1.21 (control sample) and when incubated with human saliva (tested sample). The addition of antibiotics to the above-described experimental setting, stopped Citrusin C degradation, indicating microbiologic origin of hydrolysis observed. Our results demonstrate that Citrusin C is subjected to complete degradation by salivary/oral cavity microorganisms. Extrapolation of our results allows to state that in the human oral cavity, virtually all β-D-glucosides would follow this type of hydrolysis. Additionally, a new method was developed for an in vivo rapid test of glucosidase activity in the human mouth on the tongue using fluorescein-di-β-D-glucoside as substrate. The results presented in this study serve as a proof of concept for the hypothesis that microbial hydrolysis path of β-D-glucosides begins immediately in the human mouth and releases the aglycone directly into the gastrointestinal tract.

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Quality evaluation of minoxidil topical solutions obtained from magistral pharmacies

Drug Analytical Research ◽

10.22456/2527-2616.98396 ◽

2019 ◽

Vol 3 (2) ◽

pp. 30-35

Author(s):

Karen N. Dartora ◽

Paula C. Bona ◽

Francielli L. Dos Santos ◽

Paula Bianchetti ◽

Renata Vidor Contri

Keyword(s):

Statistical Difference ◽

Quality Evaluation ◽

Quality Parameters ◽

Centrifuge Test ◽

Test Drug ◽

Organoleptic Properties ◽

Industrial Sample ◽

Drug Content ◽

Ph Values

The objective of this study was to evaluate quality parameters of magistral topical solutions containing minoxidil (A, B and C), comparing the results with the ones obtained for the industrial formulation. Organoleptic tests, evaluation of the pH and density, centrifuge test, drug content determination, comparison of indicated dosages and in vitro follicular penetration of minoxidil were performed. Regarding the organoleptic properties, differences in color and viscosity were observed between the magistral (composed of minoxidil sulfate) and the industrial formulations (composed of minoxidil base). For pH values, the magistral solutions presented considerably more acidic pH, compared to the industrial sample. For the density test, the samples with the highest ethanol percentages (B and C) presented lower density. In the centrifuge test, none of the samples showed changes. Considering the drug content test, only the industrial sample and the magistral sample C showed drug percentage within the expected (90-110%), indicating lack of correction factor determination by the magistral pharmacies. Furthermore, it was observed that the dosage indicated by the magistral pharmacies do not correspond to the dose indicated by the industry, being significantly lower. All topical solutions tested presented hair follicle penetration of minoxildil, without statistical difference. The results indicate that there is a failure in the magistral pharmacies regarding the production and the indication of dosage of minoxidil topical solutions.

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Quality evaluation with reference to clitoriacetal and in vitro antioxidant activities of Clitoria macrophylla root

Journal of Advanced Pharmaceutical Technology amp Research ◽

10.4103/japtr.japtr_67_19 ◽

2019 ◽

Vol 10 (4) ◽

pp. 169 ◽

Cited By ~ 1

Author(s):

Nijsiri Ruangrungsi ◽

Yamon Pitakpawasutthi ◽

Maneewan Suwatronnakorn ◽

Somchai Issaravanich ◽

Chanida Palanuvej

Keyword(s):

Quality Evaluation ◽

Antioxidant Activities

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Eight enteric-coated 50 mg diclofenac sodium tablet formulations marketed in Saudi Arabia: in vitro quality evaluation

BMC Research Notes ◽

10.1186/s13104-020-05270-4 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Muhammad M. Hammami ◽

Rajaa F. Hussein ◽

Reem AlSwayeh ◽

Syed N. Alvi

Keyword(s):

Quality Evaluation ◽

Diclofenac Sodium ◽

Dissolution Profile ◽

Disintegration Time ◽

United States Pharmacopoeia ◽

Weight Variation ◽

Tablet Formulations ◽

Enteric Coated

Abstract Objective To evaluate in vitro quality of enteric-coated 50 mg diclofenac sodium tablet formulations on Saudi market. Results A reference and seven generic (G1-7) formulations were commercially available in December 2019/January 2020 and were assessed within 25–75% of manufacture-expiration period. Weight variation (range as% difference from mean, n = 20), active substance content (ASC, mean (SD) as% difference from label, n = 20), hardness (mean (SD), n = 10), and friability (% weight loss, n = 20) were 97–103%, 102.0% (3.4%), 15.4 (1.1) kg, and 0.24%, respectively, for the reference. For G2-7, they were ≤ ±5%, 98.6% (4.0%) to 109.9% (1.8%), 11.9 (0.9) to 18.3 (0.8) kg, and ≤ 0.00 to 0.75%, respectively. G1 ASC, hardness, and friability were 111.3% (1.7%), 20.1 (1.7) kg, and 1.10%, respectively. Disintegration time (n = 6) and dissolution profile (n = 8) were also determined. No formulation disintegrated or released ˃ 0.1% of label ASC in 0.1 N HCl for 2 h. The reference disintegrated in 15:00 min:seconds and released a mean (range) of 100% (99–103%) of label ASC by 45 min in phosphate buffer (pH = 6.8). G1-7 disintegrated in 8:53 to 20:37 min:seconds and released 81% (69–90%) (G1) to 109%. Except for borderline performance of G1, all formulations passed in vitro quality tests according to United States Pharmacopoeia.

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In vitro propagation and quality evaluation of long-term micro-propagated and conventionally grown Fagopyrum dibotrys Hara mutant, an important medicinal plant

Journal of Medicinal Plants Research ◽

10.5897/jmpr11.1508 ◽

2012 ◽

Vol 6 (15) ◽

Cited By ~ 1

Author(s):

Caixia Chen,

Keyword(s):

Medicinal Plant ◽

In Vitro Propagation ◽

Quality Evaluation ◽

Important Medicinal Plant

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An in vitro quality evaluation of slaughterhouse by-products preserved by pickling with sulfuric acid

Biological Wastes ◽

10.1016/0269-7483(87)90062-0 ◽

1987 ◽

Vol 19 (4) ◽

pp. 281-286 ◽

Cited By ~ 4

Author(s):

S. Divakaran

Keyword(s):

Sulfuric Acid ◽

Quality Evaluation ◽

By Products

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In vitro quality evaluation of leading brands of ciprofloxacin tablets available in Bangladesh

BMC Research Notes ◽

10.1186/s13104-017-2507-y ◽

2017 ◽

Vol 10 (1) ◽

Author(s):

Md. Sahab Uddin ◽

Abdullah Al Mamun ◽

Md. Saddam Hossain ◽

Md. Asaduzzaman ◽

Md. Shahid Sarwar ◽

...

Keyword(s):

Quality Evaluation

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QbD-Based Development and Evaluation of Time-dependent Chronopharmaceutical Drug Delivery System of Amoxicillin Trihydrate for Management of Bacterial Infection

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2018.100311 ◽

2018 ◽

Vol 10 (03) ◽

Author(s):

Shyam Narayan Prasad ◽

Ashok Kumar Sahoo ◽

Abhijit V. Gothoskar

Keyword(s):

Drug Delivery ◽

Bacterial Infection ◽

Drug Delivery System ◽

Delivery System ◽

Bacterial Infections ◽

Release Kinetics ◽

Matrix Tablets ◽

Critical Material ◽

Amoxicillin Trihydrate

The present studies discuss about the quality by design (QbD)-based development and evaluation of chronomodulated release drug delivery system of amoxicillin trihydrate for management of bacterial infection. Initially, target product profile was defined and critical quality attributes were earmarked. Risk assessment study was performed for identifying the critical material attributes. Preformulation studies were carried out, and direct compression method was employed for the preparation of bilayer matrix tablets containing a delayed and a sustained release layer for preliminary optimization. Systematic formulation optimization was carried out using central composite design by selecting the concentration of Eudragit-L100 D55 and HPMCK4M. Mathematical modeling was performed and optimized compositions of the polymers were identified from the design space. Moreover, the prepared bilayer tablets were evaluated for various tablet properties including in vitro drug release study, release kinetics evaluation and characterized for FTIR, DSC, XRD, SEM studies, in vitro was-off test, antimicrobial assay and accelerated stability studies. In a nutshell, the present studies indicated the supremacy of designing a chronomodulated release bilayer tablet formulations of amoxicillin trihydrate for effective management of bacterial infections.

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