scholarly journals Estimation and Evaulation of Collagen Fibres as Stromal Componment in Various Grades of Oral Squamous Cell Carcinoma Using Picrosirius Red Stain under Polarised Microscope

2019 ◽  
Vol 4 (10) ◽  
Author(s):  
Manjushree Nayak ◽  
Jayanta Kumar Panda ◽  
Sudha Smruti Mishra ◽  
Bijay Kumar Das
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Treatment Plan ◽  
Developed Countries ◽  
Normal Mucosa ◽  
Collagen Fibres ◽  
Parallel Orientation ◽  
Picrosirius Red

Oral squamous cell carcinoma (OSSC) is the 3rd most common form of malignancy in developed countries[1]. The tumour is composed of two basic components i e, the neoplastic cells and the reactive stroma. The stromal changes which indicate the propensity of tumour cells to infiltrate and metastasise can also be used as one of the prognostic indicator[4,5]. Use of Picrosirius red (PSR) stain along with circular polarising microscope is helpful to detect spread of malignancy and guides the treatment plan and assess prognosis[11]. The present study is aimed to analyse the various aspects of collagen fibres in relation with the different grades OSSC. 30 patients of OSCC of various grades and 10 cases of normal mucosa were evaluated and data analysed statistically using sigma scan pro image analysis software(SPSS). Observation: Thirty patients (male-19 and female -11) of OSCC of age group 47-60 years were analysed. There were 14 well differentiated (WDSCC), 12 moderately differentiated (MDSCC) and 4 poorly differentiated (PDSCC) cases. When examined under polarising microscope majority (85.7%) of WDSCC and 41.67% of MDSCC showed reddish orange (RO) collagen fibres whereas in PDSCC (50%) showed green yellow (GY) collagen fibres which is statistically significant (P=0.02).In WDSCC 57.14% and in MDSCC 50% had swirl(S) arrangement whereas in PDSCC 50% had crosss hatchet (CH) and bundle arrangement. This is again statistically significant (P=0.031).While examining the orientation of collagen fibres in WDSCC 71.43% and in MDSCC, 83.33% had parallel orientation whereas in PDSCC 75% showed non parallel orientation. This orientation is statistically significant (p.008). On comparing the nature of birefringence maximum cases of WDSCC (85.71%) and MDSCC (83.33%) revealed strong birefringence whereas in PDSCC 75% exhibited weak. (statistically significant p.001). Conclusion: PSR stain under polarising microscope is an adjunct to the routine staining for studying stromal changes. The colour, arrangement, orientation & birefringence changes reflect its gradual shift from WDSCC to PDSCC and may help as a diagnostic and prognostic tool in OSCC.

scholarly journals Study of Collagen Birefringence in Different Grades of Oral Squamous Cell Carcinoma Using Picrosirius Red and Polarized Light Microscopy

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Pillai Arun Gopinathan ◽  
Ganganna Kokila ◽  
Mahadesh Jyothi ◽  
Chatterjee Ananjan ◽  
Linganna Pradeep ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Polarization Microscopy ◽  
Gradual Change ◽  
Collagen Fibres ◽  
Greenish Yellow ◽  
Picrosirius Red ◽  
Yellowish Orange

Objectives. The present study was done to evaluate birefringence pattern of collagen fibres in different grades of oral squamous cell carcinoma using Picrosirius red stain and polarization microscopy and to determine if there is a change in collagen fibres between different grades of oral squamous cell carcinoma.Materials and Methods. Picrosirius red stained 5 μm thick sections of previously diagnosed different grades of squamous cell carcinoma and normal oral mucosa were studied under polarization microscopy for arrangement as well as birefringence of collagen fibres around tumour islands.Results. It was found that thin collagen fibres increased and thick collagen fibres decreased with dedifferentiation of OSCC (P<0.0001 ). It was observed that there was change in polarization colours of thick fibres from yellowish orange to greenish yellow with dedifferentiation of OSCC indicating loosely packed fibres (P<0.0001).Conclusion. There was a gradual change of birefringence of collagen from yellowish orange to greenish yellow from well to poorly differentiated squamous cell carcinoma, indicating that there is a change from mature form of collagen to immature form as tumour progresses. Studying collagen fibres with Picrosirius red for stromal changes around tumour islands along with routine staining may help in predicting the prognosis of tumour.

Relationship Between mir15b and Sal-Like Protein 4 and Biological Behavior of Oral Squamous Cell Carcinoma

2021 ◽  
Vol 11 (2) ◽  
pp. 308-314
Author(s):  
Zengbo Wu ◽  
Yan Yan ◽  
Xianzhuo Chen ◽  
Yanling Liu ◽  
Dinggen Chen
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Normal Mucosa ◽  
Clinicopathological Features ◽  
Biological Behavior ◽  
Control Group ◽  
Tumor Tissues ◽  
Proliferation And Invasion

miR15b and SALL4 are involved in a variety of tumor progression. The roles of miR15b and SALL4 in oral squamous cell carcinoma (OSCC) remains unclear. The tumors and normal mucosa of OSCC patients were collected to detect miR15b and SALL4 level by Real-time PCR and analyze their correlation with OSCC clinicopathological features. Oral cancer Tca8113 cells were separated into control group; miR15b mimics group and miR15b inhibitor group followed by analysis of SALL4 expression, cell survival by MTT assay; cell invasion by Transwell chamber assay, as well as expression of N-cadherin and Vimentin and correlated with TNM stage, tumor volume and metastasis, and positively with differentiation TGF-β by Western blot. miR15b expression was decreased and SALL4 expression was increased in OSCC tumor tissues. miR15b was negatively degree (P < 0.05), whereas, opposite correlation of SALL4 with the above parameters was found (P < 0.05). miR15b and SALL4 were negatively correlated. MiR15b mimics significantly up-regulated MiR15b, decreased SALL4 expression, inhibited Tca8113 cell proliferation and invasion, as well as reduced N-cadherin, Vimentin and TGF-βexpression (P < 0.05). Opposite results were found in MiR15b inhibitor group. MiR15b expression is decreased and SALL 4 is increased in OSCC tumor tissues. MiR15b and SALL4 is closely related to OSCC clinicopathological features. MiR15b regulates the expression of EMT-related genes and TGF-β, thereby altering the proliferation and invasion of OSCC cells.

scholarly journals Expression of RAD51 and Its Clinical Impact in Oral Squamous Cell Carcinoma

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Yuyang Li ◽  
Jia Li ◽  
Jingchun Sun ◽  
Yingkun Liu ◽  
Dingkun Liu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Normal Skin ◽  
Clinical Stage ◽  
Prognostic Indicator ◽  
Normal Mucosa ◽  
Strong Positive Correlation ◽  
Tissue Samples

Purpose. To examine the expression of RAD51 in oral squamous cell carcinoma (OSCC) and analyze its connection with pathological grade, clinical stage, and lymphatic metastasis potential. Methods. For this study, 74 OSCC samples, 15 normal mucosa tissues, and 11 normal skin tissue samples were collected. RAD51 expression was investigated using immunohistochemistry. A follow-up visit was used to assess the prognosis of each patient. We compared RAD51 expression in oral mucosa epithelial cells (OMECs), keratinocytes, and tongue squamous cell carcinoma cells (TSCCs) by Western blot analysis. Results. RAD51 expression was higher in tumor cells than in normal mucosal tissues. In addition, RAD51 expression was associated with higher tumor differentiation (P<0.05). Also, RAD51 expression was higher (P<0.05) in patients with lymphatic metastases, and relapse rates were also higher in patients with elevated RAD51 levels (P=0.052). In addition, RAD51 expression levels were highest in the skin keratinocytes, followed by the TSCCs and OMECs. Conclusion. A strong positive correlation was found between RAD51 expression and the degree of malignancy in OSCC patients, suggesting that RAD51 could be an excellent prognostic indicator for OSCC patients.

scholarly journals SPINK7 Expression Changes Accompanied by HER2, P53 and RB1 may be Potential Biomarkers to Predict Oral Squamous Cell Carcinoma at Molecular Level

2020 ◽  
Author(s):  
Gina Penachiotti ◽  
Fabio Valdez ◽  
Wilfredo A González-Arriagada ◽  
Hector F Montes ◽  
Judith Parra ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Oral Lesion ◽  
Normal Mucosa ◽  
Morbidity Rate ◽  
High Morbidity ◽  
Less Invasive ◽  
High Morbidity Rate

Abstract Background. The oral squamous cell carcinoma (OSCC) affects more than 300,000 patients annually worldwide with a high morbidity rate (37.8%). Several tumor biomarkers have been suggested to anticipate outcome but results were poor. Changes of SPINK7 and associated proteins in precancerous oral lesions could lead to genomic instability and promote oncogenesis. Our aim was to evaluate SPINK7as apotential molecular biomarkerpredictive of OSCC stages, compared with well-known molecules altered in cancer: HER2,TP53, RB1, NFKB and CYP4B1. Methods.Oral biopsies from patientswith dysplasia (n=33), less invasive(n=28) andhighly invasiveOSCC (n=18) were collected. 20 cases with a clinical suspicion but normal mucosa confirmedwere included ascontrol. Gene expression of SPINK7, P53,RB, NFKBand CYP4B1 were quantified by qPCR.SPINK7 levels were correlated with a cohort of 330 patients from the TCGA. Also,SPINK7, HER2, TP53, and RB1, were evaluated by immunohistofluorescence. One-way Kruskal-Wallis test and Dunn's post-hocwith a p<0.05 significance were used to data analyze.Results.In OSCC, SPINK7wasdown regulated andP53, RB, NFKB and CYP4B1were up regulatedrespect tothe others groups (p<0.001). Also,SPINK7 expressionwasdiminished in patients of TCGA(p=2.10e-6). In less invasive OSCC,SPINK7 and HER2 proteinswere decreasedandTP53 and RB1 significantly increasedrespect todysplasia and highly invasivegroups (p<0.05).Conclusion. Our results suggest that SPINK7changes accompanied of HER2, P53 and RB1 can be used to classify the molecular stage of epithelial oral lesion inthe OSCC, allowing a more accuratediagnosis to molecular and histopathological level.

scholarly journals Comparison of COX2 Expression between Oral Squamous Cell Carcinoma, Leukoplakia and Normal Mucosa

2012 ◽  
Vol 13 (2) ◽  
pp. 205-209 ◽  
Author(s):  
Maryam Amirchaghmaghi ◽  
Nooshin Mohtasham ◽  
Pegah Mosannen Mozaffari
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Normal Tissue ◽  
Basal Layer ◽  
Normal Mucosa ◽  
Significant Difference ◽  
Grade 3 ◽  
Cox2 Expression

ABSTRACT Aim To compare cyclooxygenase 2 expression (COX2-E) between normal, oral leukoplakia lesions and different grades of oral squamous cell carcinoma (SCC). Materials and methods Around 90 paraffin embedded blocks consisting of 45 SCC, 15 leukoplakia and 17 controls were selected for immunohistochemistry (IHC) for detection of COX2- E. COX2-E was divided in four grades, as A (0-10%), B (11-40%), C (41-70%) and D (> 70%) cellularity. Results Mean age of the patients was 55.17 ± 18.41 (M:57.92 ± 16.87, F:52.19 ± 19.74). A significant difference was found in COX2 expression between SCC total and, basal and spinous layers of leukoplakia (p < 0.05). COX2-E in spinous layer of normal tissue was significantly lower than SCC (p = 0.000). COX2-E was significantly different in SCC grade 3 and leukoplakia (p = 0.001) and normal tissue (p = 0.000). COX2-E was significantly higher in SCC grade 3 compared to leukoplakia (basal layer) (p = 0.000). Conclusion We showed a significant higher COX2-E in SCC lesions compared to leukoplakias and normal controls. In our study COX2-E was not significantly different in SCC grades 1, 2 and 3 (p > 0.05). How to cite this article Amirchaghmaghi M, Mohtasham N, Mozaffari PM. Comparison of COX2 Expression between Oral Squamous Cell Carcinoma, Leukoplakia and Normal Mucosa. J Contemp Dent Pract 2012;13(2):205-209.

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