scholarly journals MODELAMIENTO DE LA CINÉTICA DE HIDRÓLISIS ENZIMÁTICA DE PROTEÍNAS DEL PLASMA BOVINO (MODELING OF THE KINETICS OF ENZYMATIC HYDROLYSIS OF BOVINE PLASMA PROTEINS)

Revista EIA ◽  
2013 ◽  
Vol 9 (17) ◽  
pp. 71
Author(s):  
Omar Alfredo Figueroa ◽  
José Édgar Zapata ◽  
Gail Albeiro Gutiérrez
Keyword(s):  
Kinetic Model ◽  
Plasma Proteins ◽  
Batch Reactor ◽  
Degree Of Hydrolysis ◽  
Order Kinetic ◽  
Enzyme Substrate ◽  
Bovine Plasma ◽  
Order Kinetic Model ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of

Se utilizó un modelo cinético para estudiar la velocidad de reacción en la hidrólisis de proteínas de plasma de bovino con alcalasa 2,4 L en un reactor batch. Se estudió la influencia de variables como la concentración inicial de sustrato y enzima sobre el grado de hidrólisis y se determinaron los parámetros cinéticos de la ecuación de velocidad, analizando su relación con las variables de trabajo. Se ajustó un modelo cinético de orden cero y desactivación enzimática por sustrato, de segundo orden, así como la relación directa entre la fracción enzima-sustrato y la tasa de formación de productos de hidrólisis.Abstract: A kinetic model was used to study the reaction rate of hydrolysis of bovine plasma proteins and alcalase 2.4 L, in a batch reactor. The influence of variables, such as the concentration of initial enzyme substrate and the degree of hydrolysis was studied, and kinetic parameters of the rate equation were determined by analyzing its relationship with the work variables. A zero-order kinetic model and enzyme deactivation by substrate was found, as well as the direct relationship between the fraction of enzyme-substrate and the rate of formation of hydrolysis products.

scholarly journals Analysis of electrocoagulation process efficiency of compost leachate with the first order kinetic model

2020 ◽  
Vol 10 (2) ◽  
pp. 35-40
Author(s):  
Marjana Simonič ◽  
Mirjana Čurlin ◽  
Lidija Fras Zemljič
Keyword(s):  
Kinetic Model ◽  
Removal Efficiency ◽  
Metal Removal ◽  
Batch Reactor ◽  
Process Efficiency ◽  
Order Kinetic ◽  
First Order ◽  
Order Kinetic Model ◽  
Electrocoagulation Process ◽  
Compost Leachate

Large quantities of leachate are generated from the water release during the decomposition of the biodegradable waste. The composition of compost leachate is very complex and its treatment is necessary before releasing into the environment. The possibilities of treating compost leachate by electrocoagulation have been extensively studied. The scope of this work was to investigate applicability of the first order kinetic model for degradation of metal and organic compounds from compost leachate by electrocoagulation process. Experimental results showed 75 % removal efficiency of Cu2+ and 65 % of Zn2+, while chemical oxygen demand was reduced by 36 %. According to obtained kinetic parameters, simulation of metal removal efficiency was performed in batch reactor. This way optimal electrocoagulation time which is needed for 95 % efficiency of metal removal was determined at 120th min for Zn2+ and 102nd min for Cu2+.

Kinetics of Acid Hydrolysis of Arabinogalactans

2010 ◽  
Vol 8 (1) ◽  
Author(s):  
Bright T Kusema ◽  
Chunlin Xu ◽  
Päivi Mäki-Arvela ◽  
Stefan Willför ◽  
Bjarne Holmbom ◽  
...  
Keyword(s):  
Acid Hydrolysis ◽  
Acid Concentration ◽  
Batch Reactor ◽  
Average Molecular Weight ◽  
Hydrolysis Rate ◽  
Order Kinetic ◽  
First Order ◽  
Order Kinetic Model ◽  
Kinetics Of ◽  
Hydrolysis Of

The kinetics of the acid hydrolysis of arabinogalactans (AG) was studied isothermally in a batch reactor. AG was hydrolyzed with hydrochloric acid and the main parameters established were the acid concentration (pH), temperature and AG concentration. The hydrolysis rate increased with the acid concentration (pH) and temperature. Complete hydrolysis of AG to arabinose and galactose was achieved at 90°C and pH 1 without any further degradation of the sugars. A first-order kinetic model including two simultaneous reactions for the formation of arabinose and galactose was successfully fitted to the experimental data. The rate constants and activation energies were calculated from the model. The decrease of the average molecular weight was also explained by the model.

Polarographic study of hydrolysis of [8-lysine]vasopressin and its derivatives with blood serum of pregnant women

1980 ◽  
Vol 45 (4) ◽  
pp. 1099-1108 ◽  
Author(s):  
Mikuláš Chavko ◽  
Michal Bartík ◽  
Evžen Kasafírek
Keyword(s):  
Blood Serum ◽  
Pregnant Women ◽  
Degree Of Hydrolysis ◽  
Polarographic Study ◽  
Ph Optimum ◽  
Lysine Vasopressin ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of ◽  
Vasopressin Analogues

A polarographic study of the hydrolysis of [8-lysine]vasopressin and some hormonogens of the vasopressin series with the blood serum of women in the last week of pregnancy was studied. The dependence of hydrolysis on pH (pH optimum: 7.4-7.50, substrate concentration (Km 1.2 . 10-5M), pH stability and thermal stability were determined. The rate of hydrolysis of individual vasopressin analogues decreases in the order: [8-lysine]vasopressin > Nα-glycyl-prolyl[8-lysine]-vasopressin > Nα-leucyl-[8-lysine]vasopressin > Nα-alanyl-[8-lysine]vasopressin > Nα-phenyl alanyl-[8-lysine]vasopressin > Nα-diglycyl-[8-lysine]vasopressin > Nα-prolyl-[8-lysine]vasopressin > Nα-triglycyl-[8-lysine]vasopressin > Nα-sarcosyl-glycyl-[8-lysine]vasopressin. The degree of hydrolysis gradually increases to a multiple with the length of the pregnancy in consequence of the presence of oxytocine. However, vasopressin is also hydrolysed to a small extent with the enzymes from the blood sera of non-pregnant women. Under similar analytical conditions oxytocin was not hydrolysed with the sera of non-pregnant women and therefore oxytocin is a more suitable substrate than vasopressin for polarographic determination of serum oxytocinase.

scholarly journals Investigation of Seeds and Peels of Citrullus colocynthis as Efficient Natural Adsorbent for Methylene Blue Dye

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1279
Author(s):  
Wafa Mohammed Alghamdi ◽  
Ines El Mannoubi
Keyword(s):  
Methylene Blue ◽  
Kinetic Model ◽  
Langmuir Isotherm ◽  
Order Kinetic ◽  
Citrullus Colocynthis ◽  
Natural Adsorbent ◽  
Spontaneous Process ◽  
Equilibrium Data ◽  
Order Kinetic Model ◽  
Mb Adsorption

Natural adsorbents as low-cost materials have been proved efficient for water remediation and have significant capacity for the removal of certain chemicals from wastewater. The present investigation aimed to use Citrullus colocynthis seeds (CCSs) and peels (CCPs) as an efficient natural adsorbent for methylene blue (MB) dye in an aqueous solution. The examined biosorbents were characterized using surface area analyzer (BET), scanning electron microscope (SEM), thermogravimetric analyzer (TGA) and Fourier transform infra-red (FT-IR) spectroscopy. Batch adsorption experiments were conducted to optimize the main factors influencing the biosorption process. The equilibrium data for the adsorption of MB by CCSs were best described by the Langmuir isotherm followed by the Freundlich adsorption isotherms, while the equilibrium data for MB adsorption by CCPs were well fitted by the Langmuir isotherm followed by the Temkin isotherm. Under optimum conditions, the maximum biosorption capacity and removal efficiency were 18.832 mg g−1 and 98.00% for MB-CCSs and 4.480 mg g−1 and 91.43% for MB-CCPs. Kinetic studies revealed that MB adsorption onto CCSs obeys pseudo-first order kinetic model (K1 = 0.0274 min−1), while MB adsorption onto CCPs follows the pseudo-second order kinetic model (K2 = 0.0177 g mg−1 min−1). Thermodynamic studies revealed that the MB biosorption by CCSs was endothermic and a spontaneous process in nature associated with a rise in randomness, but the MB adsorption by CCPs was exothermic and a spontaneous process only at room temperature with a decline in disorder. Based on the obtained results, CCSs and CCPSs can be utilized as efficient, natural biosorbents, and CCSs is promising since it showed the highest removal percentage and adsorption capacity of MB dye.

Dyeing mechanism and photodegradation kinetics of gardenia yellow natural colorant

2020 ◽  
pp. 004051752095848
Author(s):  
Huiyu Jiang ◽  
Xiaodong Hu ◽  
Asfandyar Khan ◽  
Jinbo Yao ◽  
Muhammad Tahir Hussain
Keyword(s):  
Kinetic Model ◽  
Cotton Fabric ◽  
Reaction Rate Constant ◽  
Second Order ◽  
Order Kinetic ◽  
Natural Colorant ◽  
Yellow Solution ◽  
Order Kinetic Model ◽  
Pseudo Second Order ◽  
Dyeing Rate

In this study, gardenia yellow solution is used to dye 100% cotton fabric. The dyeing rate curve and adsorption isotherms were recorded to explore the thermodynamic model and to calculate the corresponding parameters. A definite concentration of gardenia yellow solution was placed under the xenon arc lamp for irradiation to test its photodegradability. Absorbance of the solution was measured at different degradation times and the corresponding varying curve of the absorbance was drawn to explore the photodegradation reaction order of the natural colorant and consistent parameters were calculated. The experimental results proved that the dyeing of cotton fabric with gardenia yellow colorant followed the pseudo second order kinetic model whereas adsorption isotherm followed the Langmuir model and the photodegradation process followed the second order kinetic model. Values of different parameters were calculated: reaction rate constant k = 2.26 × 10–3 (mg · L−1)1−m h−1, the correlation coefficient R2 = 0.994, and half decay time t1/2 = 5.82 h.

Iron Chelation by Polyamidoamine Dendrimers: A Second-Order Kinetic Model for Metal–Amine Complexation

2011 ◽  
Vol 115 (46) ◽  
pp. 13534-13540 ◽  
Author(s):  
Michael R. Mankbadi ◽  
Mohamed A. Barakat ◽  
Mohamed H. Ramadan ◽  
H. Lee Woodcock ◽  
John N. Kuhn
Keyword(s):  
Kinetic Model ◽  
Iron Chelation ◽  
Second Order ◽  
Order Kinetic ◽  
Polyamidoamine Dendrimers ◽  
Order Kinetic Model ◽  
Metal Amine

Bovine plasma hydrolysates’ iron chelating capacity and its potentiating effect on ferritin synthesis in Caco-2 cells

2020 ◽  
Vol 11 (12) ◽  
pp. 10907-10912
Author(s):  
Nathalia A. Gómez-Grimaldos ◽  
Leidy J. Gómez-Sampedro ◽  
José E. Zapata-Montoya ◽  
Gabriel López-García ◽  
Antonio Cilla ◽  
...  
Keyword(s):  
Degree Of Hydrolysis ◽  
Ferritin Synthesis ◽  
Bovine Plasma ◽  
Hydrolysis Of ◽  
Ferritin Synthesis In

Bovine plasma hydrolysates with a degree of hydrolysis of 19.1% have an iron chelating capacity of 38.5 ± 0.4% and increase the synthesis of ferritin in Caco-2 cells five-fold compared to the control.

Sign in / Sign up

Export Citation Format

Share Document