scholarly journals CYTOLOGICAL CHANGES IN THE TESTIS AND EJACULATE IN CONDITIONS OF PROLONGED BLOCKADE OF THE SPERM DUCT AND ITS RECANALIZATION IN EXPERIMENT

2016 ◽  
Vol 15 (3) ◽  
pp. 38-40
Author(s):  
B. V. Hrytsuliak ◽  
V. B. Hrytsuliak ◽  
T. A. Lisova ◽  
N. P. Dolynko
Keyword(s):  
Sperm Duct ◽  
Cytological Changes

Ultrastructure of Melosira resting cell rejuvenation

1984 ◽  
Vol 42 ◽  
pp. 734-735
Author(s):  
C. E. M. Bourne ◽  
L. Sicko-Goad
Keyword(s):  
Electron Microscopy ◽  
Lake Michigan ◽  
Light And Electron Microscopy ◽  
Resting Cells ◽  
Planktonic Diatoms ◽  
Resting Cell ◽  
Vegetative Survival ◽  
Cytological Changes ◽  
Recent Attention ◽  
Freshwater Diatom

Much recent attention has been focused on vegetative survival forms of planktonic diatoms and other algae. There are several reports of extended vegetative survival of the freshwater diatom Melosira in lake sediments. In contrast to those diatoms which form a morphologically distinct resistant spore, Melosira is known to produce physiological resting cells that are indistinguishable in outward morphology from actively growing cells.We used both light and electron microscopy to document and elucidate the sequence of cytological changes during the transition from resting cells to actively growing cells in a population of Melosira granulata from Douglas Lake, Michigan sediments collected in mid-July of 1983.

High-pressure freezing and freeze substitution of teliospores of the rust fungus Gymnosporangium clavipes

1990 ◽  
Vol 48 (3) ◽  
pp. 692-693
Author(s):  
Robert W. Roberson
Keyword(s):  
High Pressure ◽  
Room Temperature ◽  
Pathogenic Fungus ◽  
Rust Fungus ◽  
Freeze Substitution ◽  
Ice Crystals ◽  
High Pressure Freezing ◽  
Thin Walled Structures ◽  
Cytological Changes ◽  
Dextran Solution

The use of cryo-techniques for the preparation of biological specimens in electron microscopy has led to superior preservation of ultrastructural detail. Although these techniques have obvious advantages, a critical limitation is that only 10-40 μm thick cells and tissue layers can be frozen without the formation of distorting ice crystals. However, thicker samples (600 μm) may be frozen well by rapid freezing under high-pressure (2,100 bar). To date, most work using cryo-techniques on fungi have been confined to examining small, thin-walled structures. High-pressure freezing and freeze substitution are used here to analysis pre-germination stages of specialized, sexual spores (teliospores) of the plant pathogenic fungus Gymnosporangium clavipes C & P.Dormant teliospores were incubated in drops of water at room temperature (25°C) to break dormancy and stimulate germination. Spores were collected at approximately 30 min intervals after hydration so that early cytological changes associated with spore germination could be monitored. Prior to high-pressure freezing, the samples were incubated for 5-10 min in a 20% dextran solution for added cryoprotection during freezing. Forty to 50 spores were placed in specimen cups and holders and immediately frozen at high pressure using the Balzers HPM 010 apparatus.

Cytological changes induced by platinum-thymine in sarcoma-180 cells: Electron microscopy study

1990 ◽  
Vol 48 (3) ◽  
pp. 290-291
Author(s):  
Gustav Ofosu
Keyword(s):  
Mammalian Cells ◽  
Antitumor Agent ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Limiting Factor ◽  
Sarcoma 180 ◽  
Electron Microscopic ◽  
Cytological Changes

Platinum-thymine has been found to be a potent antitumor agent, which is quite soluble in water, and lack nephrotoxicity as the dose-limiting factor. The drug has been shown to interact with DNA and inhibits DNA, RNA and protein synthesis in mammalian cells in vitro. This investigation was undertaken to elucidate the cytotoxic effects of piatinum-thymine on sarcoma-180 cells in vitro ultrastructurally, Sarcoma-180 tumor bearing mice were treated with intraperitoneal injection of platinum-thymine 40mg/kg. A concentration of 60μg/ml dose of platinum-thymine was used in in vitro experiments. Treatments were at varying time intervals of 3, 7 and 21 days for in vivo experiments, and 30, 60 and 120 min., 6, 12, and 24th in vitro. Controls were not treated with platinum-thymine.Electron microscopic analyses of the treated cells in vivo and in vitro showed drastic cytotoxic effect.

Tracheal Transplantation: Cytological Changes Studied by Scanning and Transmission Electron Microscopy in the Rabbit

2001 ◽  
Vol 111 (4) ◽  
pp. 657-662 ◽  
Author(s):  
Carlos Zagalo ◽  
Nuno R. Grande ◽  
Jos?? Martins dos Santos ◽  
Emanuel Monteiro ◽  
Jos?? Brito ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Transmission Electron ◽  
Tracheal Transplantation ◽  
Cytological Changes

scholarly journals STUDIES ON RENAL JUXTAGLOMERULAR CELLS

1953 ◽  
Vol 97 (3) ◽  
pp. 415-428 ◽  
Author(s):  
Phyllis Merritt Hartroft ◽  
W. Stanley Hartroft
Keyword(s):  
Sodium Chloride ◽  
Hormonal Regulation ◽  
Salt Intake ◽  
Juxtaglomerular Cells ◽  
Cytological Evidence ◽  
Secretory Cycle ◽  
Cent Sodium ◽  
Cytological Changes ◽  
Desoxycorticosterone Acetate ◽  
Sodium Metabolism

Accumulation of granules in the juxtaglomerular cells occurred in rats which were maintained for 5 to 6 weeks on a diet low in sodium, chloride. Cytological evidence suggests that this was probably a storage phase of secretion following a decrease in the rate of liberation of the granules. Administration of DCA (desoxycorticosterone acetate) to salt-deficient rats did not alter this appearance of the juxtaglomerular cells. Two per cent sodium chloride taken in the drinking water consumed for 4 weeks by similar animals caused degranulation of the juxtaglomerular cells. This effect was enhanced by DCA. DCA administered to animals on a normal salt intake produced a lesser degree of degranulation. Cytological changes in degranulated cells suggested that these represent a stage of hyperactivity in the secretory cycle produced by an increase in the rate of liberation of granules. A hypothesis is suggested that the juxtaglomerular cells are involved in the hormonal regulation of sodium metabolism and/or blood pressure.

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