scholarly journals Chronic prostatitis in a dog infected with a smooth strain of Brucella spp.

2021 ◽  
Vol 14 (1) ◽  
pp. 61-65
Author(s):  
Maria Rüegger ◽  
◽  
Maria Gorordo ◽  
Maria Luciani ◽  
Denise Pautasso ◽  
...  
Keyword(s):  
Prostate Gland ◽  
Perineal Hernia ◽  
Agglutination Test ◽  
Farm Animals ◽  
Serological Tests ◽  
Tissue Samples ◽  
Veterinary Clinic ◽  
Mixed Breed ◽  
Smooth Strain ◽  
Serum Agglutination

Dogs may be occasionally infected by smooth strains of Brucella spp. The infection is usually associated with the ingestion of contaminated material from parturition or abortion, or other tissues from infected farm animals, particularly cattle and pigs. A 6-year-old, male mixed breed dog from a rural area was admitted at a veterinary clinic for clinical examination. The dog had bilateral perineal hernia with dysuria and dyschezia, as well as small firm testicles with scrotal adhesions. Serological tests, including buffered plate antigen, serum agglutination test, and 2-mercaptoethanol test, were positive for smooth Brucella spp. strains, whereas a rapid slide agglutination test was negative for B. canis. Blood and prostate tissue samples yielded no bacterial isolates. Histopathology demonstrated interstitial lymphoplasmacytic and histiocytic infiltration of the prostate gland, with fibrosis and occasional disruption of glandular architecture. Immunohistochemistry demonstrated abundant Brucella spp. antigens in the cytoplasm of macrophages. This report supports the notion that not only B. canis, but also smooth Brucella spp. must be considered in the differential diagnosis of prostatitis in dogs.

scholarly journals A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

1976 ◽  
Vol 76 (2) ◽  
pp. 287-298 ◽  
Author(s):  
G. S. Allan ◽  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Quantitative Comparison ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Specific Antibodies ◽  
The Rose ◽  
Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

scholarly journals Leptospirosis in native mixed-breed sheep slaughtered in a semiarid region of Brazil

2017 ◽  
Vol 47 (2) ◽  
Author(s):  
Diego Figueiredo da Costa ◽  
Aline Ferreira da Silva ◽  
Arthur Willian de Lima Brasil ◽  
Ana Paula Pereira Loureiro ◽  
Fabrine Alexandre dos Santos ◽  
...  
Keyword(s):  
Bacterial Culture ◽  
Renal Tissue ◽  
Agglutination Test ◽  
Urine Samples ◽  
Semiarid Region ◽  
Tissue Samples ◽  
Mixed Breed ◽  
Leptospira Spp ◽  
Low Prevalence ◽  
Breed Sheep

ABSTRACT: Sheep breeding is an economic activity of great value for the Brazilian livestock industry, and infectious diseases can significantly compromise its productivity. The aim of this study was to detect Leptospira spp. in mixed breed sheep from a semiarid region of the State of Paraíba, Northeast Brazil. In total, 49 blood and renal tissue samples, as well as 25 urine samples, were collected from 49 sheep in the slaughter line. The microscopic agglutination test (MAT) was used to verify the presence of anti -Leptospira spp. antibodies in blood samples. Renal tissue and urine samples were analyzed by both PCR and bacterial culture. Sero reactivity was observed in 8.2% (4/49) of animals. Three renal samples were positive for leptospiral DNA by PCR although all urine samples were negative. No leptospires in pure culture were recovered from the samples tested. Low prevalence of Leptospira spp. observed by serology and PCR suggested that the rusticity of the mixed-breed sheep may have contributed to the low occurrence of leptospirosis in the semiarid region investigated.

scholarly journals Comparison of the results of some serological tests for bovine brucellosis

1978 ◽  
Vol 80 (3) ◽  
pp. 365-371 ◽  
Author(s):  
R. J. Chappel ◽  
D. J. McNaught ◽  
J. A. Bourke ◽  
G. S. Allan
Keyword(s):  
Rose Bengal ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Comparison Of The Results ◽  
The Rose ◽  
Serum Agglutination

SummaryA total of 1887 bovine sera positive to the Rose Bengal plate test were subjected to other serological tests for bovine brucellosis: the complement fixation test using warm fixation (CFTW), the serum agglutination test (SAT) and the radioimmunoassay (RIA).The SAT was generally much less sensitive than the CFTW. Many sera, however, gave positive reactions in the SAT but no reaction in the CFTW or the RIA. These SAT reactions were attributed to IgM antibody.Comparison between the results of the CFTW and the RIA led to the conclusion that 200 ng could be used as a minimum diagnostic reaction in the RIA.

COMPARISON OF EFFICIENCY OF THE RAPID WHOLE BLOOD AGGLUTINATION TEST WITH THE SERUM AGGLUTINATION TEST FOR PULLORUM DISEASE

1932 ◽  
Vol 6 (4) ◽  
pp. 381-386 ◽  
Author(s):  
Jacob Biely ◽  
William Roach
Keyword(s):  
Laboratory Test ◽  
Whole Blood ◽  
Low Cost ◽  
Agglutination Test ◽  
Practical Application ◽  
Serum Agglutination

The results obtained with the rapid whole blood agglutination test for pullorum disease, applied in the field, agree closely with the results secured with the rapid serum agglutination test, applied in the laboratory.The accuracy of the diagnosis was found to depend upon the training and experience of the technician. When the whole blood agglutination test was applied by inexperienced persons, the results obtained differed from the laboratory test by 12% as compared with a difference of 1.3% when the whole blood agglutination test was applied by an experienced technician.The rapid whole blood agglutination test was found to lend itself very readily to practical application in the field. The extremely low cost makes feasible the application and repetition of the test on a large scale.Since it is known from previous work that one agglutination test will not eliminate all carriers of pullorum disease, the rapid whole blood agglutination test should be applied several times a year until at least two successive negative tests are obtained on each bird of the flock.

A COMPARISON OF THE ROUTINE RAPID WHOLE BLOOD (STAINED ANTIGEN) AND THE ROUTINE RAPID SERUM AGGLUTINATION TESTS FOR PULLORUM DISEASE

1934 ◽  
Vol 10 (6) ◽  
pp. 798-806
Author(s):  
Jacob Biely ◽  
W. Roach
Keyword(s):  
Whole Blood ◽  
Agglutination Test ◽  
Test Laboratory ◽  
Post Mortem ◽  
Post Mortem Examination ◽  
Experiment Station ◽  
Commercial Laboratory ◽  
Serum Agglutination

Data are presented on 4,429 birds, comprising eight flocks, which were tested for pullorum disease by the whole blood agglutination test and the rapid serum agglutination test (commercial laboratory). The diagnoses agreed in the cases of 4,046 birds (97.24%) and disagreed in the cases of 122 birds (2.75%).Of the 122 birds, 43 were diagnosed as positive by the whole blood agglutination test and as negative by the rapid serum agglutination test, while 79 were diagnosed as positive by the rapid serum agglutination test and as negative by the whole blood agglutination test.Of the 122 birds, 102 were retested by the whole blood, rapid serum (Laboratory 1), and rapid serum agglutination test (Laboratory 2, (Experiment Station Laboratory)).There was a closer agreement between the diagnoses made on the basis of the whole blood and rapid serum tests (Laboratory 2) than between those made on the basis of the rapid serum (Laboratory 1) and rapid serum (Laboratory 2) tests (71.56% and 62.37% respectively).A detailed study of the retests and post-mortem examination of the 102 birds is presented.

scholarly journals Seroprevalance of Toxoplasmosis in sheep and goat: Iraq/ Sulaimania

2011 ◽  
Vol 35 (1) ◽  
pp. 16-24
Author(s):  
Lazem H. Al-Taie
Keyword(s):  
Age Groups ◽  
Neural System ◽  
Farm Animals ◽  
Economic Losses ◽  
Age Group ◽  
Serum Samples ◽  
Serological Tests ◽  
Back Ground ◽  
Qualitative Determination

Back ground: Toxoplasmosis is an important zoonosis that causes economic losses in animal herds due to abortion and stillbirth as well as changes in the reproductive and neural system of susceptible animals . Objective: The aims of the present study is to determination the prevalence of T. gondii in farm animals ( sheep& goat)of both genders and different ages in Sulaimani province by using two serological tests (ELISA and LAT). Methods: Blood samples were collected from farm animals ,142 sheep and 46 goats , of different sexes and ages. Tow different serological tests ,ELISA and LAT for qualitative determination of T. gondii antibody titer in sheep and goats serum samples. Results: The prevalence rate in sheep was 73 (51.7 %) and 82 (57 %) , and 21 (54.6 %) and 25 (54.35 %) in goats ,by ELISA and LAT respectively. The prevalence of toxoplasmosis was highest in age group 7-9 (66.6%) in sheep in compares’ with other age groups. There was no significant differences between both spp.and tow test. Conclusion: Statistical results show no significant differences between both tests (ELISA &LAT) at (P ≥ 0.05).The prevalence of toxoplasmosis was increased proportionally with the age of animals, while gender has no effect on the prevalent rate .

scholarly journals Bayesian evaluation of three serological tests for the diagnosis of bovine brucellosis in Bangladesh

2019 ◽  
Vol 147 ◽  
Author(s):  
A. K. M. A. Rahman ◽  
S. Smit ◽  
B. Devleesschauwer ◽  
P. Kostoulas ◽  
E. Abatih ◽  
...  
Keyword(s):  
Latent Class ◽  
Latent Class Model ◽  
Dairy Farm ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Brucellosis ◽  
Class Model ◽  
Serological Tests ◽  
Predictive Values ◽  
Conditional Dependence

AbstractWe evaluated the performance of three serological tests – an immunoglobulin G indirect enzyme linked immunosorbent assay (iELISA), a Rose Bengal test and a slow agglutination test (SAT) – for the diagnosis of bovine brucellosis in Bangladesh. Cattle sera (n = 1360) sourced from Mymensingh district (MD) and a Government owned dairy farm (GF) were tested in parallel. We used a Bayesian latent class model that adjusted for the conditional dependence among the three tests and assumed constant diagnostic accuracy of the three tests in both populations. The sensitivity and specificity of the three tests varied from 84.6% to 93.7%, respectively. The true prevalences of bovine brucellosis in MD and the GF were 0.6% and 20.4%, respectively. Parallel interpretation of iELISA and SAT yielded the highest negative predictive values: 99.9% in MD and 99.6% in the GF; whereas serial interpretation of both iELISA and SAT produced the highest positive predictive value (PPV): 99.9% in the GF and also high PPV (98.9%) in MD. We recommend the use of both iELISA and SAT together and serial interpretation for culling and parallel interpretation for import decisions. Removal of brucellosis positive cattle will contribute to the control of brucellosis as a public health risk in Bangladesh.

scholarly journals A serological study of Haemophilus influenzae

1937 ◽  
Vol 37 (1) ◽  
pp. 98-107 ◽  
Author(s):  
A. E. Platt
Keyword(s):  
Haemophilus Influenzae ◽  
Type A ◽  
Small Sample ◽  
Partial Purification ◽  
Serological Study ◽  
Serological Tests ◽  
Carbohydrate Fraction ◽  
Smooth Strain ◽  
Type Strains ◽  
Antigenic Heterogeneity

1. Of eighty-six strains of Haem. influenzae isolated from the naso-pharynx of normal persons, sixteen had the general characters of Pittman's “smooth” type.2. These sixteen strains and one “smooth” strain isolated from a case of meningitis were submitted to various serological tests, including the separation and partial purification of a carbohydrate fraction from the meningeal strain and from four of the sixteen strains.3. By precipitin tests carried out with these purified fractions, it was possible to identify three of the five “smooth” naso-pharyngeal strains and the meningeal strain, as belonging to Pittman's type e, and one of the “smooth” naso-pharyngeal strains to Pittman's type a.4. Some indication of grouping, within this small sample of smooth strains, was obtained by various other methods, such as precipitin tests carried out with simple saline washings or agglutination reactions; but, apart from the four type strains a, b, e and f, received from Miss Pittman, no strain could be satisfactorily typed by any of these methods. Only by using partially purified polysaccharide fractions was it possible to assign any of the “smooth” nasopharyngeal strains to their correct type.5. The testing of these naso-pharyngeal strains against antisera prepared against types a, b, e and f by direct agglutination, showed that either many of these strains contained some proportion of the smooth antigens, or that the antisera contained antibodies acting on other antigenic components, although, in relation to the type strains themselves, they appeared to be specific.6. The examination of the eighty-six strains, as a whole, revealed the extreme antigenic heterogeneity that has been noted by many previous workers.

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