Pathomorphological features and mast cell count in canine visceral hemangiosarcomas

Visceral hemangiosarcoma were analyzed at the Department of Veterinary Pathology of the Faculty of Veterinary Medicine, University of Zagreb, over a 5-year period. From a total of 52 tumor masses in a total of 31 dogs, histological growth patterns (cavernous, capillary or solid) and the amount of tumor supporting stroma were evaluated. Additionally, sections were stained with toluidine blue for the purpose of mast cell detection and their number was determined in the tumor parenchyma, tumor stroma and edges. The average age of the affected animals was 10 years, males predominated, and the tumors occurred most frequently in cross breeds, German Shepherd dogs and Labrador Retrievers. The highest number of visceral hemangiosarcoma was found in the spleen (27/52). The most common growth pattern of visceral hemangiosarcoma was solely cavernous with a mixture of cavernous and solid patterns in different proportions. Mast cells were found in 44/52 (84.6%) of the visceral hemangiosarcomas. A smaller number of mast cells were found in the tumor parenchyma, and higher number were found in the stroma and tumor edges. The number of mast cells in tumors was not significantly associated with the tumor growth pattern, but there was a positive correlation between MCC - tumor parenchyma and stroma (rs = 0.28, P<0.05), MCC - tumor stroma + edges (rs = 0.74, P<0.05) and MCC - tumor parenchyma and MCC- tumor stroma+edges (rs = 0.30, P<0.05) in all the examined tumors. In splenic tumors, there was only a significant positive correlation between MCC - stroma+edges (rs P = 0.68 P<0.05). These results suggest a higher mast cell count in tumors with more developed stromal components in canine visceral hemangiosarcomas, and certainly indicate the need for further research on their role and the factors they release in the development and progression of hemangiosarcomas.

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Correlation between clinical findings, mast cell count and interleukin 31 immunostaining in the skin of dogs with atopic dermatitis

Ciência Rural ◽

10.1590/0103-8478cr20180004 ◽

2018 ◽

Vol 48 (9) ◽

Author(s):

Bárbara Hess Rodrigues Gonçalves ◽

Bruna Dantas Matos ◽

Mariana Batista Rodrigues Faleiro ◽

Emmanuel Arnhold ◽

Moema Pacheco Chediak Matos ◽

...

Keyword(s):

Atopic Dermatitis ◽

Mast Cell ◽

Mast Cells ◽

Cell Count ◽

Plasma Cells ◽

Clinical Severity ◽

Cell Numbers ◽

Cell Counts ◽

Clinical Scores ◽

Mast Cell Count

ABSTRACT: In this study the correlation between the clinical score, mast cell count and interleukin 31 (IL-31) immunostaining in the skin of dogs with atopic dermatitis was determined. A total of 31 dogs of different breeds, from one to eight years of age, were chosen for the study. The 20 females and 11 males were categorized based on the CADESI-4 system, as having discrete, moderate or marked atopic dermatitis. Skin samples were collected from the axillary and interdigital regions and stained with hematoxylin and eosin for cytohistomorphological analyses and toluidine blue to evaluate the mast cell counts, and immunohistochemistry for the IL-31 immunostaining. Animals revealing higher atopic dermatitis scores had greater numbers of mast cells and IL-31 immunolabeled cells. More numbers of cells immunolabeled for IL-31 were evident in the axillary skin compared with the interdigital skin in dogs having this condition. A correlation was identified between the clinical scores and mast cell numbers in the interdigital region, as well as between the clinical scores and number of cells immunolabeled for IL-31 in the axillary area. A correlation was also reported between the mast cell numbers and IL-31 immunolabeled cells only in the axillary skin, and none in the interdigital regions. It was thus concluded that the mast cells and IL-31 are involved in the pathogenesis of the canine atopic dermatitis (CAD), as well as lymphocytes and plasma cells. It was also observed that the higher the degree of clinical severity of the disease, the more the numbers of mast cells and IL-31 in the skin of those animals suffering from CAD, which implies the influence of these immunological constituents on the genesis of pruritus and disease progression.

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A study on mast cell variation in neoplastic and non neoplastic disease of uterine cervix

Journal of Pathology of Nepal ◽

10.3126/jpn.v4i8.11594 ◽

2014 ◽

Vol 4 (8) ◽

pp. 658-662 ◽

Cited By ~ 1

Author(s):

N Mainali ◽

AK Sihna ◽

P Upadhyaya ◽

D Upreti

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Cell Count ◽

Uterine Cervix ◽

Statistical Significance ◽

Neoplastic Disease ◽

Neoplastic Lesion ◽

Mast Cell Count ◽

Cell Variation ◽

Relationship Of

Background: Mast cells are heterogeneous group of immune cells involved in multiple biological events. The significance of mast cells in uterine tumor surveillance has been studied with conflicting results. The presence of mast cell in tumor has been described as evidence of a host immunologic anti tumor response and if they are abundant the prognosis is good. However in other studies, with the help of different granules of mast cell, it is said to be very closely related with angiogenesis and tumor invasion. The study aims to analyze the histomorphologic changes with special reference to mast cells in different neoplastic and non neoplastic disease of uterine cervix, and also the relationship of the mast cell population with degree of anaplasia and mitotic figures.Materials and methods: Cervical biopsies received in the department of Pathology for HPE were stained with H& E stain and toludine blue for the identification of mast cellResult: Out of a total of 100 cases, 82 were non neoplastic cases with the mean mast cell count of 83.73 and mean age of patient being 44.30 year. Eighteen neoplastic cases were included which had mean mast cell count of 13.5 and mean age of 49.5 year.Conclusion: Mast cell was found to be highest in non Neoplastic lesion with increase count in polypoidal cervicitis. There was a statistical significance variation between mast cell count in neoplastic and non Neoplastic disease of the cervix. However,role of age in mast cell count was least significant.DOI: http://dx.doi.org/10.3126/jpn.v4i8.11594 Journal of Pathology of Nepal; Vol.4,No. 8 (2014) 658-662

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Evaluation of Mast Cells in Oral Potentially Malignant Disorders and Oral Squamous Cell Carcinoma

International Journal of Dentistry ◽

10.1155/2021/5609563 ◽

2021 ◽

Vol 2021 ◽

pp. 1-5

Author(s):

Ashish Shrestha ◽

Shashi Keshwar ◽

Toniya Raut

Keyword(s):

Squamous Cell Carcinoma ◽

Mast Cell ◽

Mast Cells ◽

Cell Count ◽

Oral Potentially Malignant Disorders ◽

Potentially Malignant Disorders ◽

Potentially Malignant ◽

Mast Cell Count ◽

The Mean

Background. Oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs) are epithelial-derived pathologies which share inflammation as a common initial pathogenic-inducing state. Mast cell is a key immune modulating cell which is primarily involved in initiation and propagation of inflammation. The role of mast cell in OPMDs and OSCC has been an established fact; however, its definitive pathogenic correlation is still under study. The objective of the study was to evaluate the number of mast cell in OPMDs and OSCC using special stain correlating its probable role as a promoter or retarder of OSCC. Materials and Methods. Forty-five archival histopathologically confirmed cases each of OPMD and OSCC were studied for mast cells using toluidine blue and Alcian blue-safranin stain and compared with 10 normal oral mucosal tissues. Comparisons between the mast cells count was also performed between the two special stains. Results. Among 100 cases, 67% were males and 33% were females. The mean age was 41.68 ± 13.39; 55.06 ± 12.55; and 18.4 ± 2.54 years for OPMDs, OSCC, and normal, respectively. A statistically significant increase in mast cells among OPMDs (9.88 ± 7.9) and OSCC (6.711 ± 3.94) was observed compared to normal oral mucosa. The mast cell count reduced among OSCC in comparison to OPMDs which was significant as well. The mean mast cell count for Alcian blue-safranin stain was higher than toluidine blue stain. Conclusion. There is a decrease in mean mast cell count from OPMDs to OSCC which is suggestive of protective role of mast cell. Proper quantification of mast cells using specific stains can guide to define prognosis in oral potentially malignant disorders and oral cancer.

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High fat diet and mast cell count in rat mesenterium

Cellular and Molecular Life Sciences ◽

10.1007/bf02158655 ◽

1958 ◽

Vol 14 (5) ◽

pp. 184-184 ◽

Cited By ~ 9

Author(s):

J. Fodor ◽

P. Fábry ◽

Z. Lojda

Keyword(s):

Mast Cell ◽

Cell Count ◽

High Fat Diet ◽

High Fat ◽

Mast Cell Count

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Increased Mast Cell Counts and Degranulation in Microscopic Colitis

Gastroenterology Research and Practice ◽

10.1155/2020/9089027 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

Zhikai Chi ◽

Jing Xu ◽

Romil Saxena

Keyword(s):

Mast Cell ◽

Cell Count ◽

Cell Activation ◽

Collagenous Colitis ◽

Microscopic Colitis ◽

Mast Cell Activation ◽

Mucosal Inflammation ◽

High Power Field ◽

Cell Counts ◽

Mast Cell Count

Objectives. Microscopic colitis (MC) is characterized by chronic diarrhea, normal colonoscopy findings, and mucosal inflammation in colonic biopsies and can be classified as collagenous colitis (CC) or lymphocytic colitis (LC). However, the pathogenesis of MC is largely unknown. In this study, we aimed to study mast cell counts and activation in MC. Methods. We investigated 64 biopsy samples from the surgical pathology database of Indiana University Health, which met the diagnostic criteria for CC or LC along with 20 control samples collected from 2014 to 2015. The specimens were used for the quantification of mast cells by examining the presence of intracellular and extracellular tryptase by immunohistochemistry. Results. In the lamina propria, the mast cell count was higher in both CC and LC groups than the control (mean highest count, 39/high-power field (HPF) vs. 30/HPF vs. 23/HPF; P<0.01). Extracellular tryptase was present in 10% of control subjects as compared to 41% of CC (P<0.05) and 60% of LC (P<0.001) patients. When LC patients were stratified into two groups with either <80% or >80% of fragments affected by inflammation, increased mast cell counts are only observed in the >80% involvement group compared with the control, but not the <80% involvement group. Conclusions. The increased mast cell count and degranulation are identified in MC, suggesting that mast cell activation might be involved in the pathogenesis of MC.

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Morphometric study of the fibrosis and mast cell count in the circular colon musculature of chronic Chagas patients with and without megacolon

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822003000400005 ◽

2003 ◽

Vol 36 (4) ◽

pp. 461-466 ◽

Cited By ~ 22

Author(s):

Simone Wanderley Pinheiro ◽

Adilha Misson de Oliveira Rua ◽

Renata Margarida Etchebehere ◽

Cristiane Gobbo Cançado ◽

Javier Emílio Lazo Chica ◽

...

Keyword(s):

Mast Cell ◽

Cell Count ◽

Fibrous Tissue ◽

Morphometric Study ◽

Significant Difference ◽

Mast Cell Count ◽

Distal Sigmoid

A morphometric study of the circular colon musculature was performed, in which the mast cell count was determined and the connective fibrous tissue in this layer was measured. The objective was to gain better understanding of Chagas megacolon morphology and contribute towards the knowledge of fibrosis pathogenesis in Chagas megas. An evaluation was made of 15 distal sigmoid rings from Chagas patients with megacolon (MCC), 15 without megacolon (CSMC) and 15 non-Chagas patients (NC). The rings were fixed in formol, embedded in paraffin, and 7mm thick sections were cut and stained using Azan-Heidenhain and Giemsa. The mast cell count and fibrosis were greater in the MCC group than in the CSMC and NC groups (p < 0.05; Kruskal-Wallis test) and there was no significant difference between the latter two. The fibrosis and increased mast cell count in the colon musculature of the MCC group possibly indicates that there is a relationship between mastocytosis and fibrosis, as has already been demonstrated in other pathologies.

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Correlation between testicular mast cell count and spermatogenic epithelium in non-obstructive azoospermia

International Journal of Experimental Pathology ◽

10.1111/iep.12261 ◽

2018 ◽

Vol 99 (1) ◽

pp. 22-28 ◽

Cited By ~ 5

Author(s):

Ahmed A. M. Abdel-Hamid ◽

Hoda Atef ◽

Khaled R. Zalata ◽

Atef Abdel-Latif

Keyword(s):

Mast Cell ◽

Cell Count ◽

Obstructive Azoospermia ◽

Mast Cell Count ◽

Spermatogenic Epithelium

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Does cystoscopy method affect the investigation of bladder pain syndrome/interstitial cystitis?

International Urogynecology Journal ◽

10.1007/s00192-020-04512-0 ◽

2020 ◽

Author(s):

Visha K. Tailor ◽

Ellen Morris ◽

Alka A. Bhide ◽

Ruwan Fernando ◽

Guiseppe A. Digesu ◽

...

Keyword(s):

Mast Cell ◽

Cell Count ◽

Interstitial Cystitis ◽

Muscularis Propria ◽

Pain Syndrome ◽

Bladder Pain Syndrome ◽

Detrusor Muscle ◽

Bladder Pain ◽

Significant Difference ◽

Mast Cell Count

Abstract Introduction and hypothesis Cystoscopic investigation to identify associated histological findings of increased mast cells in the detrusor muscle has been recommended by the European Society for the Study of Bladder Pain Syndrome (ESSIC) in the investigation of bladder pain syndrome/interstitial cystitis (BPS/IC). The aim of this study was to identify if the cystoscopy approach impacts the biopsy results when investigating women presenting with symptoms of BPS/IC. Methods We performed a single-centre retrospective analysis of 300 bladder biopsy reports from 2015 to 2018 from women undergoing cystoscopy for BPS/IC. Biopsies obtained using closed cup forceps through a flexible (FC) or rigid cystoscope (RC) were compared. Results Fifty-eight FC biopsies were compared with 242 RC biopsies. FC biopsies had a smaller mean diameter (1.6 mm vs 2.9 mm p < 0.01) and volume (4.1 mm3 vs 9.6 mm3p < 0.001) compared with RC biopsies. There was no significant difference in the histological depth of sampling to the muscularis propria. A total of 292 samples had CD117 immunohistochemical staining for mast cell count (MCC) analysis. The MCC/mm2 was significantly lower in FC biopsies (p < 0.01). Sixteen percent of FC samples compared with 60% of RC samples had a high MCC >28/mm2 (p < 0.01). There was no significant difference in positive microbiology culture between FC (21%) and RC (28%) sampling. Conclusion Rigid and flexible cystoscopy can be used to investigate BPS/IC as recommended by international societies. However, the biopsy method impacts the mast cell count analysis, which can influence diagnosis and management. Therefore, RC would be the optimal investigation.

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