Optimum Conditions for Phenol Oxidase (Laccase) Production from  Azotobacter chroococcum by Submerged Fermentation

Phenol oxidases (laccase), copper-containing oxidase enzymes, are found in many plants, fungi and microorganisms. Many factors that influence laccase production from Azotobacter chroococcum are determined in this study, these factors are: carbon source, nitrogen source, pH, incubation temperature and incubation period. The results showed that the best carbon and nitrogen sources for laccase production are sucrose and yeast extract respectively, and the best factors for laccase production conditions are pH 7.0 with an incubation period of 6 days at temperature 30 Cᵒ.

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Optimising carbon and nitrogen sources for Azotobacter chroococcum growth

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb10.1484 ◽

2011 ◽

Vol 10 (15) ◽

pp. 2951-2958 ◽

Cited By ~ 3

Author(s):

Gutieacute rrez Rojas Ivonne ◽

Beatriz Torres Geraldo Ana ◽

Moreno Sarmiento Nubia

Keyword(s):

Nitrogen Sources ◽

Azotobacter Chroococcum ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen

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Optimum conditions for Prodigiosin production by Serratia marcescens S11

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2011.5.3.180 ◽

2011 ◽

Vol 5 (3) ◽

pp. 34-40

Author(s):

Abdulkareem Jasim ◽

Hameed M. Jasim ◽

Isra'a M. Dhahi

Keyword(s):

Serratia Marcescens ◽

Culture Medium ◽

Casein Hydrolysate ◽

Brain Heart Infusion ◽

Nitrogen Sources ◽

Optimum Conditions ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Phosphate Source ◽

Initial Medium

Different nutritional and cultural factors were studied to determine the optimum conditions for prodigiosin production by Serratia marcescens S11 in a batch culture of brain-heart infusion broth medium. These factors include carbon source and its concentration, nitrogen source and its concentration, phosphate source, temperature and pH. Results showed that the optimum conditions for prodigiosin production were achieved when the production medium was supplemented with olive oil and casein hydrolysate as a carbon and nitrogen sources respectively in a concentration of 1.5% for broth, KH2PO4 as a phosphate source at initial medium pH8, and incubation at 28°C for 24 hours. Under these optimal conditions, prodigiosin activity produced by Serratia marcescens S11 in culture medium was increased from 200 U/cell before optimization to 3000 U/cell.

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Production of Xylanase from Arthrobacter sp. MTCC 6915 Using Saw Dust As Substrate under Solid State Fermentation

Enzyme Research ◽

10.4061/2011/696942 ◽

2011 ◽

Vol 2011 ◽

pp. 1-7 ◽

Cited By ~ 10

Author(s):

Sevanan Murugan ◽

Donna Arnold ◽

Uma Devi Pongiya ◽

P.M. Narayanan

Keyword(s):

Enzyme Activity ◽

Solid State ◽

Incubation Temperature ◽

Nitrogen Sources ◽

Optimal Temperature ◽

Carbon And Nitrogen Sources ◽

Xylanase Production ◽

Carbon And Nitrogen ◽

Saw Dust ◽

Production Temperature

Saw dust was used as substrate for xylanase production from Arthrobacter sp. MTCC 6915. The study of period of incubation, temperature, pH, carbon, and nitrogen sources for xylanase production was optimized. Xylanase production was found to be optimum at an incubation period of 96 hrs (117.0 U/mL), temperature 30°C (105.0 U/mL), and pH 9.0 (102.9 U/mL). The results showed that the xylanase production was found to be higher in the presence of carboxymethylcellulose (176.4 U/mL) and dextrose (126.0 U/mL). It was also observed that peptone (170.1 U/mL) and beef extract (161.7 U/mL) supported maximum xylanase production.The enzyme was characterized and found to be fairly active at pH 9 (764.4 U/mL) and temperature 60°C (819 U/mL). Even in the present study, a major difference in the production temperature (30°C) and optimal temperature (60°C) of the enzyme activity was observed. However, the pH of the production media and the enzyme activity were found to be the same (pH 9).

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Optimum the Fermentation Condition to Produce the Polysaccharide of Hericium erinaceus with Tofu Whey

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.998-999.275 ◽

2014 ◽

Vol 998-999 ◽

pp. 275-278

Author(s):

Zuo Fa Zhang ◽

Guo Ying Lv ◽

Xue Jiang ◽

Lei Fa Fan

Keyword(s):

Incubation Temperature ◽

Environmental Parameters ◽

Nitrogen Sources ◽

Fermentation Condition ◽

Cultivation Medium ◽

Carbon And Nitrogen Sources ◽

Hericium Erinaceus ◽

Carbon And Nitrogen ◽

Potato Extract ◽

Optimum Production

The present study was to evaluate the effects of certain nutrients and environmental parameters on growth and production of polysaccharide of Hericium erinaceus with tofu whey. The effect of different carbon and nitrogen sources, temperature, pH and incubation period on the production of polysaccharide in flask cultures by H. erinaceus was investigated. Glucose and potato extract were found to be the most suitable carbon and nitrogen source, respectively. Optimum production of polysaccharide required glucose in the cultivation medium with a concentration of 8.0 g/L. On the other hand, the highest production of polysaccharide was obtained in a cultivation medium containing 12.0 g/L of potato extract. Furthermore, the incubation temperature at 27 °C, pH 5.5, rotation rate 180 and seven days of the incubation were found to be the optimum for maximum production of polysaccharide of H. erinaceus.

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Enhancement of Extracellular Pullulanase Production by Raoultella planticola DSMZ 4617 Using Optimized Medium Based on Sago Starch

The Open Biotechnology Journal ◽

10.2174/1874070700903010001 ◽

2009 ◽

Vol 3 (1) ◽

pp. 1-8 ◽

Cited By ~ 8

Author(s):

Siew Ling Hii ◽

Tau Chuan Ling ◽

Rosfarizan Mohamad ◽

Arbakariya B. Ariff

Keyword(s):

Incubation Temperature ◽

Nitrogen Sources ◽

Sago Starch ◽

Cultivation Medium ◽

Carbon And Nitrogen Sources ◽

Raoultella Planticola ◽

Carbon And Nitrogen ◽

Initial Culture ◽

Czapek Medium ◽

Medium Formulation

Enhancement of pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium formulation was investigated in batch fermentation using 500-mL shake flask. The fermentations were carried out, firstly, to search for a suitable cultivation medium for enzyme production and followed by the evaluations on the influence of carbon and nitrogen sources and also initial culture pHs on the secretion of pullulanase by this bacterium. The modified mineral Czapek medium was found suitable to produce substantially high activity of pullulanase (320 times higher) as compared to ‘Ohba-Ueda’ medium. This bacterium was found superior in pullulanase production using sago starch and peptone as carbon and nitrogen sources, respectively. Using the optimized medium, the bacterium produced 0.95 U/mL of pullulanase at initial culture pH of 7 and incubation temperature of 30oC.

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Production and Characterization of Polyhydroxyalkanoates from Lactic Acid Bacteria Isolated from Dairy Wastewater, Fermented Cow Milk and ‘Ogi’

Journal of Advances in Microbiology ◽

10.9734/jamb/2020/v20i930279 ◽

2020 ◽

pp. 31-46

Author(s):

Wakil Sherifah Monilola ◽

Oluwatobi Esther Makinde

Keyword(s):

Liquid Medium ◽

Lactobacillus Casei ◽

Incubation Temperature ◽

Nitrogen Sources ◽

Agitation Speed ◽

Dairy Wastewater ◽

Cow Milk ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Pha Production

Aims: This study aimed at screening, producing and characterizing polyhydroxyalkanoates (PHA) from lactic acid bacteria (LAB) isolated from dairy wastewater, fermented cow milk and “ogi”. Place and Duration of Study: Department of Microbiology, Faculty of Science, University of Ibadan, Ibadan between August 2018 and February 2019. Methodology: The initial screening of isolated LAB was carried out by Sudan Black staining method followed by secondary screening on liquid medium. Isolates that tested positive for Sudan Black stain and had the highest PHA yield from liquid medium was used for further analysis. The cell walls of selected isolates were lysed with sodium hypochlorite and PHA extracted using chloroform. Optimization of PHA production was carried out using different carbon and nitrogen sources, incubation temperature, pH and agitation speed. Extracted PHA was characterized by FTIR spectroscopy and the microstructure and surface morphology were observed using Scanning Electron Microscope. Results: Seven isolates tested positive for Sudan Black stain of which two isolates identified as Lactobacillus plantarum CW10 and Lactobacillus casei WWD3 had the highest PHA yield from liquid medium. From the optimization experiment, highest PHA production was observed in Lactobacillus plantarum CW10 (20.5%) and Lactobacillus casei WWD3 (19.7%) when glucose and ammonium sulphate was used as carbon and nitrogen sources respectively, and at pH 6, agitation speed of 200 rpm and incubation temperature of 35oC and 40oC. The spectra of extracted PHA as characterized by FTIR showed absorption peaks for the carbonyl, C-H, -OH, aliphatic -CH3 and ester groups that are characteristics of PHA. The microstructure and surface morphology shows grains that are pseudo-spherical in shape with fairly regular distribution. Conclusion: There is an appreciable production of PHA from the Lactobacillus strains and can be considered as part of the choice of organisms for PHA production in commercial quantity.

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Ability of the fungus Aureobasidium pullulans to produce polysaccharide (pullulan) with presence of different Carbon and Nitrogen sources

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2014.8.1.311 ◽

2014 ◽

Vol 8 (1) ◽

pp. 67-70

Author(s):

Zahraa Ibraheem Muhammed Al_dabbagh ◽

Muhammed Basheer Ismaeil Kassim

Keyword(s):

Amino Acid ◽

Carbon Source ◽

Nitrogen Source ◽

Incubation Period ◽

Aureobasidium Pullulans ◽

Nitrogen Sources ◽

Extracellular Polysaccharide ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Amino Acid Glycine

The effect of incubation period, different carbon and nitrogen sources on the production of the extracellular polysaccharide pullulan by Aureobasidium pullulansNRRL58560 was examined. The results showed that the maximum production of pullulan was obtained 13.76 after 96 hours of incubation. Sucrose as a carbon source gave the highest production of pullulan 31.92 g/l while the highest biomass was obtained when starch was used as a carbon source 16.30 g/l. The amino acid glycine as a nitrogen source gave the highest production of pullulan 34.61 g/l and the highest production of biomass 14.49 g/l was obtained when ammonium chloride was used.

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