Nylon Oxygen Barrier Tubing Reduces Biofouling in Beer Draught Lines

Beer draught lines are frequently contaminated with biofilm-forming microorganisms, which forces retailers to spend considerable time and money cleaning and replacing lines. In light of this financial burden, draught tubing composition was examined for its role in the prevention of biofouling in beer lines. Three types of draught tubing - vinyl, polyethylene, and nylon barrier - were inoculated with a combination of biofilm-forming microorganisms (Hafnia paralvei, Raoultella planticola, Pediococcus damnosus and Saccharomyces cerevisiae) and used to simulate a bar environment for sixteen weeks. Following simulation, the degree of biofouling in each draught line was determined by spectrophotometry and microscopy. Absorption values and fluorescence images showed that nylon barrier tubing was superior to the other lines at resisting biofilm maturation.These results suggest that tubing composition plays a significant role in the prevention of biofilm formation in beer draught lines and supports the adoption of nylon barrier tubing as an effective strategy against biofouling in a variety of applications.

Péritonite à Raoultella planticola chez un patient en dialyse péritonéale automatisée 48 heures après ensablement d’une piste équestre

Nous rapportons ici un cas de péritonite à Raoultella planticola multisensible chez un patient immunodéprimé en dialyse péritonéale automatisée. Le patient avait travaillé à l’ensablement d’une piste équestre 48 heures auparavant. L’évolution clinique et biologique a été rapidement favorable sous antibiothérapie par ciprofloxacine. La ciprofloxacine est remplacée dans un second temps par de l’amoxyclavulanate en raison d’une tendinopathie achiléenne. L’antibiothérapie est poursuivie pour une durée totale de 3 semaines avec une bonne réponse clinique.Aucun autre cas de péritonite à raoultella n’a été rapporté à ce jour auprès du registre de Dialyse Péritonéale de Langue Française.

Complete Genome Sequence of a Canadian Strain of Raoultella planticola with Metal and Antimicrobial Resistance Genes

Raoultella planticola is a Gram-negative opportunistic bacterial pathogen associated with hospital-acquired infections in humans. Here, we report the complete genome sequence of one Raoultella planticola strain isolated from Canadian wastewater treatment facilities containing one chromosome and four plasmids with four antimicrobial resistance (AMR) genes and four metal resistance gene clusters.

Bacteremic cholangitis due to Raoultella planticola complicating intrahepatic bile duct stricture 5 years post-laparoscopic cholecystectomy: a case report

Background Raoultella Planticola is a facultative anaerobic, gram-negative, water- and soil-dwelling rod bacterium rarely reported as a cause of human disease. However, the number of reported R. planticola infections is growing, without a concomitant increase in research on the microbe or its pathogenesis. Previous genomic studies demonstrating genetic similarities between R. planticola and Klebsiella pneumoniae suggest that capsule biosynthesis, mucoid phenotype, biofilm production, and lipopolysaccharide (endotoxin) synthesis may all be potential virulence factors of R. planticola. We present a unique case of R. planticola infection of the biliary tract 5 years after biliary surgery in a patient with no previously documented risk factors. We also use in silico techniques to predict virulence factors of R. planticola. Case presentation This case report is the first to discuss a R. planticola infection in the biliary tract of late onset post-surgery (5 years) in a Caucasian patient with no previously documented risk factors. Conclusions An in-depth search of the current literature did not yield other similar cases of R. planticola infections. Moreover, to the best of our knowledge, our case is the first case of R. planticola isolated from post-endoscopic retrograde cholangiopancreatography (ERCP) as part of biliary sepsis not associated with gastroenteritis. The late onset of the infection in our patient and the results of the in silico analysis suggest that R. planticola may have survived exposure to the host immune system through the creation of an intracellular biofilm or in a non-culturable but viable state (NCBV) for the 5-year period. The in silico analysis also suggests that biofilms, enterobactin, and mucoid phenotype may play a role in the pathogenesis of R. planticola. However, further research is needed to illuminate the significance of pili, capsule biosynthesis, and lipopolysaccharide (LPS) in the virulence of R. planticola. Lastly, as our patient did not have any risk factors previously associated with R. planticola, we suggest that biliary tract stricture, cholecystitis, and prior surgery may be possible novel risk factors.