scholarly journals Study of genetic variability of Ribes L. representatives grown in Belarus

2017 ◽  
Vol 1 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Volha Anatol’evna Mezhnina ◽  
Aksana Yuryeuna Urbanovich
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Molecular Level ◽  
Diagnostic Value ◽  
Dna Identification ◽  
Black Currant

Abstract The research aims to study the genetic variability of Ribes L. representatives grown in Belarus, and to reveal a set of DNAmarkers for its DNA-identification. We formed a set of SSR-markers that possess rather high diagnostic value and allow identifying of black currant and gooseberry varieties at a molecular level and can be recommended for DNA-identification of those cultures.

Assessment of genetic variability among selected species of Apocynaceae

Biologia ◽  
2011 ◽  
Vol 66 (1) ◽  
Author(s):  
Tariq Mahmood ◽  
Anna Iqbal ◽  
Nazia Nazar ◽  
Ishrat Naveed ◽  
Bilal Abbasi ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Catharanthus Roseus ◽  
Rapd Markers ◽  
Molecular Level ◽  
Ornamental Plants ◽  
Morphological Characters ◽  
Randomly Amplified Polymorphic Dna ◽  
Medicinal Uses ◽  
Alstonia Scholaris ◽  
Thevetia Peruviana

AbstractFamily Apocynaceae is an economically important family grown as ornamental plants and many wild species have medicinal uses as well. The aim of the present study was to understand the level and pattern of genetic variability among the selected individuals of Apocynaceae. For this purpose, three species of different genera of Apocynaceae, Thevetia peruviana, Alstonia scholaris and Catharanthus roseus, were collected from Rawalpindi and Quaid-i-Azam University forest, Islamabad. To evaluate the level of polymorphism within the species and members of different species, randomly amplified polymorphic DNA (RAPD) markers were used. A series of OPC RAPD primers were used; only six primers of OPC series gave amplification. Highest genetic variation at interspecific and intraspecific levels was shown by OPC 9 and the lowest polymorphism was observed in OPC 4. The data was analyzed by using software Statistica 5.5. In total 105 monomorphic and 272 polymorphic bands were produced from all primers. Therefore, out of 322 amplified products, 26% were monomorphic and 68% were polymorphic. Low genetic diversification was observed both at intraspecific and interspecific level. At the molecular level Alstonia scholaris and Catharanthus roseus (subfamily Plumerioideae) appeared in a group and Thevetia peruviana (subfamily Rauvolfoideae) formed another group, confirming the classification based on morphological characters.

scholarly journals Elucidating Genetic Diversity in Apricot (Prunus armeniaca L.) Cultivated in the North-Western Himalayan Provinces of India Using SSR Markers

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2668
Author(s):  
Zahid Nabi Sheikh ◽  
Vikas Sharma ◽  
Rafiq Ahmad Shah ◽  
Shilpa Raina ◽  
Maha Aljabri ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Polymorphic Information Content ◽  
Genetic Parameter ◽  
Nutritional Composition ◽  
Prunus Armeniaca ◽  
Jammu And Kashmir ◽  
Expected Heterozygosity ◽  
Wild Apricot ◽  
Prunus Armeniaca L

Apricot (Prunus armeniaca L.) is an important temperate fruit crop worldwide. The availability of wild apricot germplasm and its characterization through genomic studies can guide us towards its conservation, increasing productivity and nutritional composition. Therefore, in this study, we carried out the genomic characterization of 50 phenotypically variable accessions by using SSR markers in the erstwhile States of Jammu and Kashmir to reveal genetic variability among accessions and their genetic associations. The genetic parameter results revealed that the number of alleles per locus (Na) ranged from 1 to 6 with a mean Na value of 3.89 and the mean effective number of alleles (Ne) per locus 1.882 with a range of 1.22 to 2. Similarly, the polymorphic information content (PIC) values ranged from 0.464 to 0.104. The observed heterozygosity (Ho) (0.547) was found to have higher than expected heterozygosity (He) (0.453) with average heterozygosity of 0.4483. The dendrogram clustered genotypes into three main clades based on their pedigree. The population structure revealed IV sub-populations with all admixtures except the III sub-population, which was mainly formed of exotic cultivars. The average expected heterozygosity (He) and population differentiation within four sub-populations was 1.78 and 0.04, respectively, and explained 95.0% of the total genetic variance in the population. The results revealed that the SSR marker studies could easily decrypt the genetic variability present within the germplasm, which may form the base for the establishment of good gene banks by reducing redundancy of germplasm, selection of parents for any breeding program.

Characterization of Resistance of Peanut to Puccinia arachidis

2013 ◽  
Vol 14 (1) ◽  
pp. 31 ◽  
Author(s):  
Imana L. Power ◽  
Albert K. Culbreath ◽  
Barry L. Tillman
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Collaborative Research ◽  
Field Studies ◽  
The United States ◽  
Breeding Lines ◽  
Plant Introductions ◽  
Puccinia Arachidis ◽  
Arachis Hypogaea L

Peanut rust, caused by Puccinia arachidis Speg, is an important foliar disease of peanut (Arachis hypogaea L.) in tropical countries. The best option for disease management is host resistance. The objectives of this project included characterizing peanut genotypes for resistance to P. arachidis, assessing the genetic variation of newly developed Collaborative Research and Support Program (CRSP) peanut breeding lines, and assessing genetic variability among P. arachidis populations. In field studies conducted over 2010-2011, several CRSP breeding lines demonstrated varying levels of rust resistance. Detached leaf assays were used to examine the components of resistance to P. arachidis. Few significant differences were observed in these studies. We used SSR markers to characterize newly developed CRSP breeding lines, plant introductions, and commonly grown cultivars. The SSR markers used detected polymorphisms but were not able to distinguish resistant from susceptible peanut genotypes. Sequences of the 5.8S-ITS2-28S region of P. arachidis isolates collected from different regions in the United States and other countries do not indicate high genetic variability among the populations. Accepted for publication 23 September 2013. Published 25 November 2013.

Assessment of Genetic Variability in Long-lived Cupressus sempervirens var. horizontalis Using SSR Markers

2012 ◽  
Author(s):  
Korori A. A. Soudabeh ◽  
Azadfar Davoud ◽  
Shirvany Anoushirvan ◽  
Valipour K. Hossein ◽  
Matinizadeh Mohammad
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Cupressus Sempervirens

Genetic variability of Indonesian landraces of Vigna subterranea: Morphological characteristics and molecular analysis using SSR markers

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
MUHAMMAD FAUZAN FARID ALHAMDI ◽  
Asep Setiawan ◽  
Satriyas Ilyas ◽  
Wai Kuan Ho
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Molecular Analysis ◽  
Block Design ◽  
Leaf Shape ◽  
Morphological Characteristics ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Alternative Source ◽  
Randomized Block Design

Abstract. Alhamdi MFF, Setiawan A, Ilyas S, Ho WK. 2020. Genetic variability of Indonesian landraces of Vigna subterranea: The morphological characteristics and molecular analysis using SSR markers. Biodiversitas 21: 3929-3937. Bambara groundnut (Vigna subterranea (L.) Verdc.) is a potential grain, which can be considered as an alternative source of protein and carbohydrate. Due to unavailability of commercial bambara groundut cultivar in Indonesia, the characterization of bambara groundnut landraces is an important step before developing cultivar with traits of interest. The objective of the research was to access genetic variability of Indonesian landraces of bambara groundnut with different seed coat colors based on morphological and molecular markers. The experiment was arranged as split-plot in a complete randomized block design with the main plot was cultivation methods and the sub plot was landraces. There were differences in leaf shape and pod shape among the landraces. There were two main clusters of Indonesian landraces of bambara groundnut with 88.28% similarity. The first cluster was Cream, Brown Sumedang, Black Sumedang and Black Tasikmalaya, and the second cluster was Black Sukabumi, Brown Gresik, Black Madura, and Black Gresik. The result based on SSR marker with capillary electrophoresis indicated Black Gresik and Black Madura landraces were different from other Indonesian landraces.  Cream Sumedang or Brown Sumedang from the first cluster and Black Gresik or Brown Gresik from the second cluster have the farthest distances for developing improved variety of bambara groundnut.

scholarly journals Fingerprinting and genetic variability in cork oak (Quercus suber L.) elite trees using ISSR and SSR markers

2007 ◽  
Vol 64 (7) ◽  
pp. 773-779 ◽  
Author(s):  
Aimara Löpez-Aljorna ◽  
Maria Angeles Bueno ◽  
Itziar Aguinagalde ◽  
Juan Pedro Martín
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Quercus Suber ◽  
Cork Oak ◽  
Elite Trees

scholarly journals Genetic Diversity and Population Structure of Traditional Greek and Cypriot Melon Cultigens (Cucumis melo L.) Based on Simple Sequence Repeat Variability

HortScience ◽  
2009 ◽  
Vol 44 (7) ◽  
pp. 1820-1824 ◽  
Author(s):  
Emmanouil N. Tzitzikas ◽  
Antonio J. Monforte ◽  
Abdelhak Fatihi ◽  
Zacharias Kypriotakis ◽  
Tefkros A. Iacovides ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Variability ◽  
Ssr Markers ◽  
Structure Analysis ◽  
Simple Sequence Repeat ◽  
Cucumis Melo ◽  
Sequence Repeat ◽  
Population Structure Analysis ◽  
Simple Sequence

Seventeen simple sequence repeat (SSR) markers were used to assess the genetic diversity and population structure among traditional Greek and Cypriot melon cultigens (Cucumis melo L.). All SSR markers were polymorphic with a total number of 81 alleles, whereas all cultigens could be distinguished with at least one SSR, except cultigens 43 and 41. Reference accessions showed larger genetic variability with an average of four alleles per locus and 0.65 gene of diversity compared with an average of 2.47 alleles per locus and 0.30 of gene diversity for the Greek/Cypriot cultigens. Observed heterozygosity was very low, indicating a lack of outcrossing, at least in recent times. Unrooted neighbor-joining tree analysis and population structure analysis clustered the cultigens and the reference genotypes into five groups. All cultigens could be distinguished; the Cypriot cultigens were more closely related to the inodorus ‘Piel de Sapo’, whereas the Greek cultigens were located in an intermediate position between the inodorus ‘Piel de Sapo’ and the cantalupensis ‘Védrantais’. The cultigen ‘Kokkini’ was the most divergent among the Greek and Cypriot cultigens. This association between geographic origin and genetic similarity among Greek and Cypriot cultigens indicates geographic isolation. Most of the cultivars from the same cultivar group (i.e., inodorus, cantalupensis) clustered together, but some exceptions were found, suggesting that former inodorus landraces would have been transformed to cantalupensis as a result of intercrossing and further selection by farmers. Results of population structure analysis support mixing between cantalupensis and inodorus. ‘Agiou Basileiou’, an inodorus cultigen, was assigned to the subpopulation IV/II of which II is a pure cantalupensis subpopulation. Greek and Cypriot melon cultigens were developed from a broader germplasm base than western Mediterranean cultivars and exhibited useful for melon breeding programs genetic variability.

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