Assessment of genetic variability among selected species of Apocynaceae

Biologia ◽  
2011 ◽  
Vol 66 (1) ◽  
Author(s):  
Tariq Mahmood ◽  
Anna Iqbal ◽  
Nazia Nazar ◽  
Ishrat Naveed ◽  
Bilal Abbasi ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Catharanthus Roseus ◽  
Rapd Markers ◽  
Molecular Level ◽  
Ornamental Plants ◽  
Morphological Characters ◽  
Randomly Amplified Polymorphic Dna ◽  
Medicinal Uses ◽  
Alstonia Scholaris ◽  
Thevetia Peruviana

AbstractFamily Apocynaceae is an economically important family grown as ornamental plants and many wild species have medicinal uses as well. The aim of the present study was to understand the level and pattern of genetic variability among the selected individuals of Apocynaceae. For this purpose, three species of different genera of Apocynaceae, Thevetia peruviana, Alstonia scholaris and Catharanthus roseus, were collected from Rawalpindi and Quaid-i-Azam University forest, Islamabad. To evaluate the level of polymorphism within the species and members of different species, randomly amplified polymorphic DNA (RAPD) markers were used. A series of OPC RAPD primers were used; only six primers of OPC series gave amplification. Highest genetic variation at interspecific and intraspecific levels was shown by OPC 9 and the lowest polymorphism was observed in OPC 4. The data was analyzed by using software Statistica 5.5. In total 105 monomorphic and 272 polymorphic bands were produced from all primers. Therefore, out of 322 amplified products, 26% were monomorphic and 68% were polymorphic. Low genetic diversification was observed both at intraspecific and interspecific level. At the molecular level Alstonia scholaris and Catharanthus roseus (subfamily Plumerioideae) appeared in a group and Thevetia peruviana (subfamily Rauvolfoideae) formed another group, confirming the classification based on morphological characters.

scholarly journals Analysis of Genetic Diversity among Selected Grasspea (Lathyrus sativus L.) Genotypes Using RAPD Markers

2007 ◽  
Vol 62 (11-12) ◽  
pp. 869-874 ◽  
Author(s):  
Durga P. Barik ◽  
Laxmikanta Acharya ◽  
Arup K. Mukherjee ◽  
Pradeep K. Chand
Keyword(s):  
Rapd Markers ◽  
Molecular Level ◽  
Crop Improvement ◽  
Dna Amplification ◽  
The Other ◽  
Randomly Amplified Polymorphic Dna ◽  
Similar Morphology ◽  
Molecular Weights ◽  
Breeding Programmes ◽  
Lathyrus Sativus L

Randomly amplified polymorphic DNA (RAPD) technique was applied to assess the genetic variability among five selected genotypes of grasspea. Out of 30 random decamer primers tested for the present investigation 20 showed reproducible DNA amplification. A total of 257 loci were amplified of which 159 were polymorphic including 57 genotype-specific unique bands. Amplicons had molecular weights ranging from 3.0 kb to 0.1 kb. Majority amplicons were shared by most of the genotypes which indicated a very narrow genetic gap between them. The dendrogram constructed on the basis of RAPD data showed two clusters. The local genotype collected from Nayagarh was grouped along with IC-120451 and IC-120453, sharing a common node at an 82% similarity level. The other genotypes, IC-120478 and IC-120487, were located in the second clade having a common node at 84% similarity level. The investigation showed that though all the genotypes of grasspea were of apparently similar morphology there exists polymorphism at the molecular level, which can be exploited in breeding programmes aimed at crop improvement.

scholarly journals Use of RAPD Markers to Analyze Genetic Variability of Introgressed Brassica Lines

2016 ◽  
Vol 14 (1) ◽  
pp. 122-133
Author(s):  
Md. Harun-Or-Rashid ◽  
Md. Shafikur Rahman ◽  
Sudhir Chandra Nath ◽  
S S R M Mahe Alam Sorwar ◽  
Md. Tanvir Ahmed
Keyword(s):  
Cluster Analysis ◽  
Genetic Variability ◽  
Genetic Distance ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Lower Proportion ◽  
Randomly Amplified Polymorphic Dna ◽  
Polymorphic Loci ◽  
Parental Lines ◽  
The Cross

Seven individuals of introgressed Brassica lines (Binasarisha-5/Daulot) and two of their parental lines were used for this study to estimate genetic variability using three randomly amplified polymorphic DNA (RAPD) markers (61AB10G1, 72AB10G12 and 73AB10T13). A total of 23 clear bands were scored, of which 21 (91.30%) bands were proved to be polymorphic. The highest proportion of polymorphic loci and gene diversity values were 43.48% and 0.187, respectively in the line five of Binasarisha-5/Daulot. The lower proportion of polymorphic loci and gene diversity values were 8.70% and 0.034; 8.70% and 0.026 in the line seven of the cross and one parent, Daulot, respectively. The co-efficient of gene differentiation (Gst) and gene flow (Nm) values were 0.677 and 0.237, found respectively from the Popgene analysis. Result of cluster analysis indicated that the nine accessions were capable of being classified into two major groups - one consists of only one parent Daulot (Brassica juncea) while another consists of Binasarisha-5 (Brassica napus) and all introgressed lines of C6 generation (treated with colchicine in C1 generation) resulted from the cross B. napus and B. juncea. Introgressed line seven and Binasarisha-5 showed the lowest genetic distance of 0.077. Higher similarity was found between Binasarisha-5 and introgressed progenies. Introgressed line one and Daulot showed the highest genetic distance of 0.709, which can be used as germplasm for breeding program that aim to improve Brassica. It was concluded that RAPD markers can be used for the study of molecular characterization and diversity in Brassica.The Agriculturists 2016; 14(1) 122-133

scholarly journals Molecular Marker Analysis of Vitis vinifera `Albariño' and some Similar Grapevine Cultivars

1998 ◽  
Vol 123 (5) ◽  
pp. 842-848 ◽  
Author(s):  
M. Dolores Loureiro ◽  
M. Carmen Martínez ◽  
Jean-Michel Boursiquot ◽  
Patrice This
Keyword(s):  
Genetic Variability ◽  
Molecular Marker ◽  
Vitis Vinifera ◽  
Microsatellite Loci ◽  
Rapd Markers ◽  
Dna Polymorphisms ◽  
Clonal Variation ◽  
Randomly Amplified Polymorphic Dna ◽  
Grape Cultivar ◽  
Molecular Marker Analysis

`Albariño' (Vitis vinifera L.) is an important grape cultivar in Spain, morphologically diverse but subject to much misnaming. The objectives of the present work were to correct some of the more common misnamings concerning `Albariño' and to evaluate the genetic variability within this cultivar by analyzing DNA polymorphisms using randomly amplified polymorphic DNA (RAPD) markers and microsatellite techniques. Several accessions of `Albariño' (16 accessions from Misión Biológica de Galicia, one accession from El Encin, one accession from Rancho de la Merced), related cultivars (`Alvarinho', `Caíño blanco', `Cainho branco', `Loureiro'), and cultivars presumably identical to misnomers (`Savagnin blanc' and `Gewürztraminer') were analyzed using 20 RAPD markers and six microsatellite loci. Both techniques revealed polymorphism among `Albariño', `Caíño blanco', `Albariño' from Rancho de la Merced and `Loureiro'. No polymorphism was detected among the 16 `Albariño' accessions from Galicia, the `Albariño' accession from El Encin and `Alvarinho', nor among the `Albariño' accession from Rancho de la Merced, `Savagnin blanc' and `Gewürztraminer', nor between `Caíño blanco' and `Cainho branco'. These results enabled us to clarify the main misnomers concerning these cultivars. The absence of polymorphism among the true `Albariño' accessions did not allow the detection of any clonal variation. The suitability of both techniques for defining the cultivar level for grapevine is discussed.

Identification of Hydrangeaceae accessions of wild origin from Jeju, Korea, using molecular markers

2010 ◽  
Vol 8 (3) ◽  
pp. 235-241 ◽  
Author(s):  
Young Hee Joung ◽  
Jeung Keun Suh ◽  
Nam Sook Lee ◽  
Sang Mi Eum ◽  
Ik-Young Choi ◽  
...  
Keyword(s):  
Rapd Markers ◽  
Large Subunit ◽  
Ornamental Plants ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Bisphosphate Carboxylase ◽  
Apical Buds ◽  
Ribulose 1

Hydrangea and Schizophragma are popular horticultural and ornamental plants. This study was designed to identify unknown accessions of Hydrangeaceae that have leaf morphological characters different from Hydrangeaanomala subsp. petiolaris and Schizophragma hydrangeoides in Jeju, Korea. A total of 29 accessions of Hydrangea and Schizophragma from the wild were collected and analyzed using randomly amplified polymorphic DNA (RAPD) and single nucleotide polymorphisms (SNPs) from the ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene. Morphological characteristics of leaves and shoot apices of several accessions were also examined. Based on the RAPD markers, SNPs of the (rbcL) gene and morphological characteristics of apical buds, the unknown accessions were identified as H. anomala var. petiolaris.

Genetic variation and species identification among selected leeches (Hirudinea) revealed by RAPD markers

Biologia ◽  
2012 ◽  
Vol 67 (4) ◽  
Author(s):  
Aleksander Bielecki ◽  
Kornelia Polok
Keyword(s):  
Genetic Variation ◽  
Genetic Variability ◽  
Multidimensional Scaling ◽  
Rapd Markers ◽  
Genetic Parameters ◽  
Morphological Characters ◽  
Species Differentiation ◽  
North Eastern ◽  
Evolutionary Studies ◽  
First Time

AbstractLeech taxonomy is based on unstable morphological characters. The overall level of genetic variability and species differentiation is unknown. Using the RAPD assays genetic diversities and genetic similarities were estimated in twelve species collected in North-Eastern Poland and representing three families and five subfamilies. Ten primers revealed 204 reproducible bands. Genetic diversities varied from 0.099 to 0.219 classifying studied species among variable invertebrates. Total 45 markers comprised 22% of all amplified bands were unique for species thus enabling their identification. Genetic similarities among species (0.528–0.811) evidenced several stages of differentiation, which is mirrored in the current taxonomy. The UPGMA and multidimensional scaling (nMDS) based on our RAPDs are congruent and reflected traditional division into “Rhynchobdellida” and Arhynchobdellida. The RAPD approach proved to be an effective tool in population and evolutionary studies of leeches. For the first time, genetic parameters were estimated enabling to compare leeches with outcomes from other animals.

Study of genetic diversity in wild raspberry (Rubus idaeus L.) germplasm collection using morphological characters and RAPD markers

Biologija ◽  
2008 ◽  
Vol 54 (2) ◽  
pp. 66-74 ◽  
Author(s):  
Jolanta Patamsytė ◽  
Donatas Žvingila ◽  
Juozas Labokas ◽  
Virgilijus Baliuckas ◽  
Laimutė Balčiūnienė ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Germplasm Collection ◽  
Morphological Characters ◽  
Rubus Idaeus

scholarly journals Metal Accumulation Profile of Catharanthus roseus (L.) G.Don and Celosia argentea L. with EDTA Co-Application

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 598
Author(s):  
Muneeba Qurban ◽  
Cyrus Raza Mirza ◽  
Aqib Hassan Ali Khan ◽  
Walid Khalifa ◽  
Mustapha Boukendakdji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Catharanthus Roseus ◽  
Metal Uptake ◽  
Metal Accumulation ◽  
Metal Tolerance ◽  
Plant Biomass ◽  
Ethylenediaminetetraacetic Acid ◽  
Ornamental Plants ◽  
Environmental Deterioration ◽  
Celosia Argentea

The problem of metal-induced toxicity is proliferating with an increase in industrialization and urbanization. The buildup of metals results in severe environmental deterioration and harmful impacts on plant growth. In this study, we investigated the potential of two ornamental plants, Catharanthus roseus (L.) G.Don and Celosia argentea L., to tolerate and accumulate Ni, Cr, Cd, Pb, and Cu. These ornamental plants were grown in Hoagland’s nutrient solution containing metal loads (50 µM and 100 µM) alone and in combination with a synthetic chelator, ethylenediaminetetraacetic acid (EDTA) (2.5 mM). Plant growth and metal tolerance varied in both plant species for Ni, Cr, Cd, Pb, and Cu. C. roseus growth was better in treatments without EDTA, particularly in Ni, Cr, and Pb treatments, and Pb content increased in all parts of the plant. In contrast, Cd content decreased with EDTA addition. In C. argentea, the addition of EDTA resulted in improved plant biomass at both doses of Cu. In contrast, plant biomass reduced significantly in the case of Ni. In C. argentea, without EDTA, root length in Cd and Cu treatments was significantly lower than the control and other treatments. However, the addition of EDTA resulted in improved growth at both doses for Pb and Cu. Metal accumulation in C. argentea enhanced significantly with EDTA addition at both doses of Cu and Cd. Hence, it can be concluded that EDTA addition resulted in improved growth and better metal uptake than treatments without EDTA. Metal accumulation increased with EDTA addition compared to treatments without EDTA, particularly for Pb in C. roseus and Cu and Cd in C. argentea. Based on the present results, C. roseus showed a better ability to phytostabilize Cu, Cd, and Ni, while C. argentea worked better for Ni, Cd, Cu, and Pb.

scholarly journals Genetic variation among South Brazilian accessions of Lippia alba Mill. (Verbenaceae) detected by ISSR and RAPD markers

2009 ◽  
Vol 69 (2) ◽  
pp. 375-380 ◽  
Author(s):  
MF. Manica-Cattani ◽  
J. Zacaria ◽  
G. Pauletti ◽  
L. Atti-Serafini ◽  
S. Echeverrigaray
Keyword(s):  
Genetic Variability ◽  
Rapd Markers ◽  
Molecular Data ◽  
Chemical Characteristics ◽  
Hierarchical Analysis ◽  
Rio Grande Do Sul ◽  
Medicinal Species ◽  
Lippia Alba ◽  
Very High ◽  
Issr Primers

Twenty-seven accessions of Lippia alba Mill. collected in Rio Grande do Sul state, Brazil, were analysed by ISSR and RAPD markers to evaluate their genetic variability and relationships. Six ISSR primers and four RAPD primers generated 120 amplified fragments, most of which were polymorphics. The overall genetic variability among accessions was very high when compared with other plant species. The hierarchical analysis of molecular data (UPGMA) showed low relationship between accessions, and no grouping between accessions of the same chemotype. Canonical functions allowed identifying some variables related with the chemical characteristics of the essential oils. Both ISSR and RAPD markers were efficient to address the genetic diversity of L. alba, and may contribute to the conservation and breeding of this increasingly important aromatic and medicinal species.

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