Assessment of Genetic Variability in Long-lived Cupressus sempervirens var. horizontalis Using SSR Markers

2012 ◽  
Author(s):  
Korori A. A. Soudabeh ◽  
Azadfar Davoud ◽  
Shirvany Anoushirvan ◽  
Valipour K. Hossein ◽  
Matinizadeh Mohammad
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Cupressus Sempervirens

scholarly journals Elucidating Genetic Diversity in Apricot (Prunus armeniaca L.) Cultivated in the North-Western Himalayan Provinces of India Using SSR Markers

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2668
Author(s):  
Zahid Nabi Sheikh ◽  
Vikas Sharma ◽  
Rafiq Ahmad Shah ◽  
Shilpa Raina ◽  
Maha Aljabri ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Polymorphic Information Content ◽  
Genetic Parameter ◽  
Nutritional Composition ◽  
Prunus Armeniaca ◽  
Jammu And Kashmir ◽  
Expected Heterozygosity ◽  
Wild Apricot ◽  
Prunus Armeniaca L

Apricot (Prunus armeniaca L.) is an important temperate fruit crop worldwide. The availability of wild apricot germplasm and its characterization through genomic studies can guide us towards its conservation, increasing productivity and nutritional composition. Therefore, in this study, we carried out the genomic characterization of 50 phenotypically variable accessions by using SSR markers in the erstwhile States of Jammu and Kashmir to reveal genetic variability among accessions and their genetic associations. The genetic parameter results revealed that the number of alleles per locus (Na) ranged from 1 to 6 with a mean Na value of 3.89 and the mean effective number of alleles (Ne) per locus 1.882 with a range of 1.22 to 2. Similarly, the polymorphic information content (PIC) values ranged from 0.464 to 0.104. The observed heterozygosity (Ho) (0.547) was found to have higher than expected heterozygosity (He) (0.453) with average heterozygosity of 0.4483. The dendrogram clustered genotypes into three main clades based on their pedigree. The population structure revealed IV sub-populations with all admixtures except the III sub-population, which was mainly formed of exotic cultivars. The average expected heterozygosity (He) and population differentiation within four sub-populations was 1.78 and 0.04, respectively, and explained 95.0% of the total genetic variance in the population. The results revealed that the SSR marker studies could easily decrypt the genetic variability present within the germplasm, which may form the base for the establishment of good gene banks by reducing redundancy of germplasm, selection of parents for any breeding program.

Characterization of Resistance of Peanut to Puccinia arachidis

2013 ◽  
Vol 14 (1) ◽  
pp. 31 ◽  
Author(s):  
Imana L. Power ◽  
Albert K. Culbreath ◽  
Barry L. Tillman
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Collaborative Research ◽  
Field Studies ◽  
The United States ◽  
Breeding Lines ◽  
Plant Introductions ◽  
Puccinia Arachidis ◽  
Arachis Hypogaea L

Peanut rust, caused by Puccinia arachidis Speg, is an important foliar disease of peanut (Arachis hypogaea L.) in tropical countries. The best option for disease management is host resistance. The objectives of this project included characterizing peanut genotypes for resistance to P. arachidis, assessing the genetic variation of newly developed Collaborative Research and Support Program (CRSP) peanut breeding lines, and assessing genetic variability among P. arachidis populations. In field studies conducted over 2010-2011, several CRSP breeding lines demonstrated varying levels of rust resistance. Detached leaf assays were used to examine the components of resistance to P. arachidis. Few significant differences were observed in these studies. We used SSR markers to characterize newly developed CRSP breeding lines, plant introductions, and commonly grown cultivars. The SSR markers used detected polymorphisms but were not able to distinguish resistant from susceptible peanut genotypes. Sequences of the 5.8S-ITS2-28S region of P. arachidis isolates collected from different regions in the United States and other countries do not indicate high genetic variability among the populations. Accepted for publication 23 September 2013. Published 25 November 2013.

Genetic variability of Indonesian landraces of Vigna subterranea: Morphological characteristics and molecular analysis using SSR markers

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
MUHAMMAD FAUZAN FARID ALHAMDI ◽  
Asep Setiawan ◽  
Satriyas Ilyas ◽  
Wai Kuan Ho
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Molecular Analysis ◽  
Block Design ◽  
Leaf Shape ◽  
Morphological Characteristics ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Alternative Source ◽  
Randomized Block Design

Abstract. Alhamdi MFF, Setiawan A, Ilyas S, Ho WK. 2020. Genetic variability of Indonesian landraces of Vigna subterranea: The morphological characteristics and molecular analysis using SSR markers. Biodiversitas 21: 3929-3937. Bambara groundnut (Vigna subterranea (L.) Verdc.) is a potential grain, which can be considered as an alternative source of protein and carbohydrate. Due to unavailability of commercial bambara groundut cultivar in Indonesia, the characterization of bambara groundnut landraces is an important step before developing cultivar with traits of interest. The objective of the research was to access genetic variability of Indonesian landraces of bambara groundnut with different seed coat colors based on morphological and molecular markers. The experiment was arranged as split-plot in a complete randomized block design with the main plot was cultivation methods and the sub plot was landraces. There were differences in leaf shape and pod shape among the landraces. There were two main clusters of Indonesian landraces of bambara groundnut with 88.28% similarity. The first cluster was Cream, Brown Sumedang, Black Sumedang and Black Tasikmalaya, and the second cluster was Black Sukabumi, Brown Gresik, Black Madura, and Black Gresik. The result based on SSR marker with capillary electrophoresis indicated Black Gresik and Black Madura landraces were different from other Indonesian landraces.  Cream Sumedang or Brown Sumedang from the first cluster and Black Gresik or Brown Gresik from the second cluster have the farthest distances for developing improved variety of bambara groundnut.

scholarly journals Fingerprinting and genetic variability in cork oak (Quercus suber L.) elite trees using ISSR and SSR markers

2007 ◽  
Vol 64 (7) ◽  
pp. 773-779 ◽  
Author(s):  
Aimara Löpez-Aljorna ◽  
Maria Angeles Bueno ◽  
Itziar Aguinagalde ◽  
Juan Pedro Martín
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Quercus Suber ◽  
Cork Oak ◽  
Elite Trees

scholarly journals Genetic Diversity and Population Structure of Traditional Greek and Cypriot Melon Cultigens (Cucumis melo L.) Based on Simple Sequence Repeat Variability

HortScience ◽  
2009 ◽  
Vol 44 (7) ◽  
pp. 1820-1824 ◽  
Author(s):  
Emmanouil N. Tzitzikas ◽  
Antonio J. Monforte ◽  
Abdelhak Fatihi ◽  
Zacharias Kypriotakis ◽  
Tefkros A. Iacovides ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Variability ◽  
Ssr Markers ◽  
Structure Analysis ◽  
Simple Sequence Repeat ◽  
Cucumis Melo ◽  
Sequence Repeat ◽  
Population Structure Analysis ◽  
Simple Sequence

Seventeen simple sequence repeat (SSR) markers were used to assess the genetic diversity and population structure among traditional Greek and Cypriot melon cultigens (Cucumis melo L.). All SSR markers were polymorphic with a total number of 81 alleles, whereas all cultigens could be distinguished with at least one SSR, except cultigens 43 and 41. Reference accessions showed larger genetic variability with an average of four alleles per locus and 0.65 gene of diversity compared with an average of 2.47 alleles per locus and 0.30 of gene diversity for the Greek/Cypriot cultigens. Observed heterozygosity was very low, indicating a lack of outcrossing, at least in recent times. Unrooted neighbor-joining tree analysis and population structure analysis clustered the cultigens and the reference genotypes into five groups. All cultigens could be distinguished; the Cypriot cultigens were more closely related to the inodorus ‘Piel de Sapo’, whereas the Greek cultigens were located in an intermediate position between the inodorus ‘Piel de Sapo’ and the cantalupensis ‘Védrantais’. The cultigen ‘Kokkini’ was the most divergent among the Greek and Cypriot cultigens. This association between geographic origin and genetic similarity among Greek and Cypriot cultigens indicates geographic isolation. Most of the cultivars from the same cultivar group (i.e., inodorus, cantalupensis) clustered together, but some exceptions were found, suggesting that former inodorus landraces would have been transformed to cantalupensis as a result of intercrossing and further selection by farmers. Results of population structure analysis support mixing between cantalupensis and inodorus. ‘Agiou Basileiou’, an inodorus cultigen, was assigned to the subpopulation IV/II of which II is a pure cantalupensis subpopulation. Greek and Cypriot melon cultigens were developed from a broader germplasm base than western Mediterranean cultivars and exhibited useful for melon breeding programs genetic variability.

scholarly journals Genetic variability in peas (Pisum sativum L.) from Turkey asssessed with molecular and morphological markers

2019 ◽  
Vol 31 (1) ◽  
pp. 101-116
Author(s):  
Fatih Hanci
Keyword(s):  
Genetic Variability ◽  
Pisum Sativum ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Morphological Characteristics ◽  
Principal Component ◽  
Morphological Markers ◽  
Pisum Sativum L ◽  
Euclidean Distances ◽  
Simple Sequence

AbstractThe aim of this study was to identify the molecular and morphological characteristics of Turkish pea accessions (Pisum sativum L.). The genetic diversity among 130 Turkish landraces and 2 commercial varieties in a total of 132 pea accessions was assessed with 14 simple sequence repeat (SSR) markers. Forty-eight (48) polymorphic alleles were identified using 14 SSR markers. The pairwise Dice coefficients of similarity between accessions ranged from 0.091 to 0.960. The polymorphism information content (PIC) value ranged from 0.585 to 0.861. Overall, 50 morphological traits were evaluated. Cluster analysis was carried out on a matrix of Euclidean distances. The accessions were divided into three main groups. Principal component analysis (PCA) was used to identify the weight of each morphological characteristic. According to the results, the highest eigenvalue was observed in PC-I (13.88) followed by PC-II (11.42), and PC-III (7.32). The first fifteen PCs with eigenvalues > 1 explained 74.08% of the variability. The results showed that the molecular markers were useful and polymorphic, sufficient to allocate all the evaluated accessions. This research has provided significant insights into the genetic variability of Turkish pea accessions.

scholarly journals Genetic variability of Verbascum populations from metal polluted and unpolluted sites

Genetika ◽  
2015 ◽  
Vol 47 (1) ◽  
pp. 245-251 ◽  
Author(s):  
Gordana Danilovic ◽  
Filis Morina ◽  
Zlatko Satovic ◽  
Ljiljana Prokic ◽  
Dejana Pankovic
Keyword(s):  
Genetic Variability ◽  
Genetic Differentiation ◽  
Ssr Markers ◽  
Environmental Conditions ◽  
Metal Content ◽  
Plant Adaptation ◽  
Suitable Material ◽  
Adaptation Mechanisms

Mullein (Verbascum) plants have extensive distribution and can grow in variable environmental conditions. Seed was collected from mullein plants grown at 4 locations, two metals contaminated and two metal uncontaminated areas. Genetic variability of progeny was examined. Populations collected from unpolluted areas were genetically more similar than those collected from polluted areas as revealed by RAPD and SSR markers and UPGMA analysis. The results indicate that there is genetic differentiation between examined populations and therefore they represent suitable material for further investigation of plant adaptation mechanisms to increased metal content.

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