scholarly journals Statistical Optimization of Medium Components of Xylanase Production by Irpex lacteus BAFC 1168 strain F under liquid fermentation

2015 ◽  
Vol 5 (1) ◽  
pp. 559-569 ◽  
Author(s):  
Gabriela Verónica Díaz
Keyword(s):  
Central Composite Design ◽  
Xylanase Activity ◽  
White Rot ◽  
White Rot Fungus ◽  
Irpex Lacteus ◽  
Xylanase Production ◽  
Liquid Fermentation ◽  
Medium Components ◽  
The Media ◽  
Central Composite

The enhanced xylanase production of white rot fungus Irpex lacteus BAFC 1168 strain F using a liquid fermentation culture was investigated. The media nutrients were screened by a Plackett-Burman design and from the analysis of variance, carboxy-methylcellulose, urea and peptone were found to be the most positive and significant nutrient components. Hence, these three variables were selected for further optimization using a five-level central composite design in response surface methodology. The validation of the model experiment was conducted reaching 8.29 U/mL of xylanase activity when the composition medium was 1.6 g/L of CMC, 0.84 g/L of urea and 2 g/L of peptone. Optimum temperature and pH conditions on xylanase activity were also determined using a central composite design. It was reached a maximum xylanase activity of 8.36 U/mL when temperature and pH were 42.8°C and 5.6, respectively.

Valorization of de-oiled cakes for xylanase production and optimization using central composite design by Trichoderma koeningi isolate

2017 ◽  
Vol 42 (3) ◽  
Author(s):  
Ramesh Bandikari ◽  
Umamahesh Katike ◽  
Naga Sivudu Seelam ◽  
Vijaya Sarathi Reddy Obulam
Keyword(s):  
Central Composite Design ◽  
18S Rrna ◽  
Fermentation Medium ◽  
Xylanase Production ◽  
Corn Cobs ◽  
Medium Components ◽  
The Media ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

AbstractAimEvaluation of the medium components and conditions for the optimization of xylanase production in submerged fermentation byMethodswas identified by the 18s rRNA. NaOH pre-treated corn cobs were used in order to reduce the crystallinity of corn cobs. De-oiled cakes proximate composition was analyzed according to AOAC. Plackett-Burman design (PBD) was used to screen the 19 media variables that affect xylanase production and optimized the media by central composite design (CCD).ResultsThe isolate was identified asConclusionNaOH pre-treated corn cobs were used with mustard cake powder as fermentation medium constituent to induce the xylanase production. From the results we conclude that mustard cake induced the xylanase production.

scholarly journals Alkali treatment of fungal pretreated wheat straw for bioethanol production

Bioethanol ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
María García-Torreiro ◽  
Miguel Álvarez Pallín ◽  
María López-Abelairas ◽  
Thelmo A. Lu-Chau ◽  
Juan M. Lema
Keyword(s):  
Wheat Straw ◽  
Alkali Treatment ◽  
Alkaline Treatment ◽  
White Rot ◽  
White Rot Fungus ◽  
Process Conditions ◽  
Alkali Pretreatment ◽  
Irpex Lacteus ◽  
C 30 ◽  
Pretreated Wheat Straw

AbstractBioconversion of lignocellulosic materials into ethanol requires an intermediate pretreatment step for conditioning biomass. Sugar yields from wheat straw were previously improved by the addition of a mild alkali pretreatment step before bioconversion by the white-rot fungus Irpex lacteus. In this work, an alternative alkaline treatment, which significantly reduces water consumption, was implemented and optimized. Sugar recovery increased 117% with respect to the previously developed alkaline wash process at optimal process conditions (30°C, 30 minutes and 35.7% (w/w) of NaOH). In order to further reduce operational costs, a system for alkali recycling was implemented. This resulted in the treatment of 150% more wheat straw using the same amount of NaOH. Finally, enzymatic hydrolysis was optimized and resulted in a reduction of enzyme dose of 33%.

Irpex lacteus, a white-rot fungus with biotechnological potential — review

2009 ◽  
Vol 54 (5) ◽  
pp. 375-390 ◽  
Author(s):  
Č. Novotný ◽  
T. Cajthaml ◽  
K. Svobodová ◽  
M. Šušla ◽  
V. Šašek
Keyword(s):  
White Rot ◽  
White Rot Fungus ◽  
Irpex Lacteus ◽  
Biotechnological Potential

scholarly journals Characterization of Acetylxylan Esterase from White-Rot Fungus Irpex lacteus

2019 ◽  
Vol 66 (4) ◽  
pp. 131-137
Author(s):  
Sangho Koh ◽  
Seika Imamura ◽  
Naoto Fujino ◽  
Masahiro Mizuno ◽  
Nobuaki Sato ◽  
...  
Keyword(s):  
White Rot ◽  
White Rot Fungus ◽  
Irpex Lacteus ◽  
Acetylxylan Esterase

Bioremediation of the heavy metal complex dye Isolan Dark Blue 2SGL-01 by white rot fungus Irpex lacteus

2011 ◽  
Vol 198 ◽  
pp. 198-205 ◽  
Author(s):  
Duraisamy Kalpana ◽  
Jae Hong Shim ◽  
Byung-Taek Oh ◽  
Kalaiselvi Senthil ◽  
Yang Soo Lee
Keyword(s):  
Heavy Metal ◽  
Metal Complex ◽  
White Rot ◽  
White Rot Fungus ◽  
Irpex Lacteus ◽  
Dark Blue

Iron and calcium translocation from pure gypsum and iron-amended gypsum by two brown rot fungi and a white rot fungus

Holzforschung ◽  
2008 ◽  
Vol 62 (6) ◽  
Author(s):  
Jonathan S. Schilling ◽  
Kaitlyn M. Bissonnette
Keyword(s):  
Building Materials ◽  
Woody Debris ◽  
White Rot Fungi ◽  
Brown Rot ◽  
White Rot ◽  
White Rot Fungus ◽  
Irpex Lacteus ◽  
Brown Rot Fungi ◽  
Ca Oxalate ◽  
Iron And Calcium

AbstractWood-degrading fungi commonly grow in contact with calcium (Ca)-containing building materials and may import Ca and iron (Fe) from soil into forest woody debris. For brown rot fungi, imported Ca2+may neutralize oxalate, while Fe3+may facilitate Fenton-based degradation mechanisms. We previously demonstrated, in two independent trials, that degradation of spruce by wood-degrading fungi was not promoted when Ca or Fe were imported from gypsum or metallic Fe, respectively. Here, we tested pine wood with lower endogenous Ca than the spruce blocks used in prior experiments, and included a pure gypsum treatment and one amended with 1% with FeSO4. Electron microscopy with microanalysis verified that brown rot fungiSerpula himantioidesandGloeophyllum trabeumand the white rot fungusIrpex lacteusgrew on gypsum and produced iron-free Ca-oxalate crystals away from the gypsum surface. Wood cation analysis verified significant Fe import by both brown rot isolates in Fe-containing treatments. Wood degradation was highest in Fe-gypsum-containing treatments for all three fungi, although only wood degraded byI. lacteushad significant Ca import. We suggest that Fe impurities may not exacerbate brown rot, and that both brown and white rot fungi may utilize Ca-containing materials.

scholarly journals Factors Governing Degradation of Phenol in Pharmaceutical Wastewater by White-rot Fungi: A Batch Study

2015 ◽  
Vol 9 (1) ◽  
pp. 93-99 ◽  
Author(s):  
M. Bernats ◽  
T. Juhna
Keyword(s):  
Synthetic Medium ◽  
Biomass Concentration ◽  
White Rot Fungi ◽  
Total Phenol ◽  
White Rot ◽  
White Rot Fungus ◽  
Optimal Conditions ◽  
Pharmaceutical Wastewater ◽  
Irpex Lacteus ◽  
Low Efficiency

Phenol is a major contaminant in the industrial water effluent, including pharmaceutical wastewaters. Although several physic-chemical methods for removal of phenol exist, they are of high cost, low efficiency, and generate toxic by-products. Thus, there is a need to develop technologies for biological removal of phenol from wastewater. In this study, the degradation of phenol in pharmaceutical wastewater by monoculture of white-rot fungi was studied. The degradation rate of total phenol in batch flasks by four fungal monocultures of Trametes versicolor, Phanerochaete chrysosporium, Gloeophyllum trabeum and Irpex lacteus in synthetic medium was compared. The results showed that white-rot fungus T.Versicolor was the most effective of the species. Further selection tests of optimal conditions of biomass concentration, pH and temperature were done, indicating that optimal conditions of degradation are at pH 5-6, temperature 25 °C, and biomass inoculum 10% (v/v). Under optimal conditions, total phenol was reduced by 93%, concentration of total phenol decreasing from 420±12 mg/l to 29±1 mg/l in seven days, with T.Versicolor specie. This study suggested that biological treatment with fungi may effectively be used as a pre-treatment stage for removal of phenol before polishing wastewater with conventional biological methods.

scholarly journals Media optimization for C-phycocyanin production in Plectonema sp. using response surface methodology and central composite design

2020 ◽  
Vol 11 (3) ◽  
pp. 3897-3904
Author(s):  
Arbab Husain ◽  
Fahad Khan ◽  
Khwaja Osama ◽  
Sadaf Mahfooz ◽  
Adeeba Shamim ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Citric Acid ◽  
Central Composite Design ◽  
Response Surface ◽  
Nostoc Muscorum ◽  
Therapeutic Implications ◽  
Medium Components ◽  
Anabaena Sp ◽  
Different Strains ◽  
Central Composite

Cyanobacteria represent the richest sources of phycobiliproteins with especial reference to C-phycocyanin (C-PC), which in turn holds exhaustive therapeutic implications. Screening of several cyanobacterial strains namely Anabaena sp., Nostoc muscorum, Cylindrospermum sp., Plectonema sp., Scytonema sp., Spirulina sp., Synechococcous sp. and Tolypothrix sp. was carried out for their C-PC producing capacity, however the produced quantity of C-PC varies greatly among different strains. Owing to the crucial role of different media constituents on productivity of C-PC the current study was designed to optimize most appropriate media composition for augmented CPC production by selected superior producer. 36 factorial central composite design (CCD) dependent response surface methodology (RSM) was utilized to estimate the important medium components attributed with influencing C-PC productivity. RSM analysis of five independent coded factors including Na2CO3, K2HPO4, NaNO3, citric acid and EDTA were analyzed preceded by recognition of efficient variables for algal components production by Plectonema sp. Investigation of results revealed that the eminent medium components were NaCO3 (0.4 g/L); NaNO3 (0.5 g/L); K2HPO4 (2.8 g/L); citric acid (0.08 g/L) and EDTA (0.01 g/L) respectively. The optimized combination yielded 0.5536 mg/ml of C-PC. The increment of C-PC yield is R-Sq = 88.2%. Thus, our study led to the recognition of critical nutritional component that can be used further for enhanced productivity of C-PC.

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