Quantitative assessment of mast cells and expression of IgE protein and mRNA for IgE and interleukin 4 in the gastrointestinal tract of healthy dogs and dogs with inflammatory bowel disease

The pathomechanism of inflammatory bowel disease (IBD) has not yet been fully demonstrated. However, it is well known that mast cells are present in the gastrointestinal tract, suggesting that mast cells may take part in it. So, we investigated the number of mast cells in IBD, such as ulcerative colitis (UC) and eosinophilic colitis, and showed that the number of mast cells was increased in the inflammatory lesions. We also presented a case of UC which was treated successfully with an antiallergic drug, tranilast. Furthermore, possible new approaches to treating the disease with immunomodulators including suplatast are introduced. However, our investigations were performed with a limited number of patients with IBD, and additional further studies are required to confirm the findings.

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Alteration of Interleukin 4 Production Results in the Inhibition of T Helper Type 2 Cell–Dominated Inflammatory Bowel Disease in T Cell Receptor α Chain–Deficient Mice

Journal of Experimental Medicine ◽

10.1084/jem.190.5.607 ◽

1999 ◽

Vol 190 (5) ◽

pp. 607-616 ◽

Cited By ~ 75

Author(s):

Hideki Iijima ◽

Ichiro Takahashi ◽

Daisuke Kishi ◽

Jin-Kyung Kim ◽

Sunao Kawano ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

T Cells ◽

T Cell ◽

T Cell Receptor ◽

Bowel Disease ◽

Cell Receptor ◽

Interleukin 4 ◽

T Helper ◽

Inflammatory Bowel ◽

Α Chain

T cell receptor α chain–deficient (TCR-α−/−) mice are known to spontaneously develop inflammatory bowel disease (IBD). The colitis that develops in these mice is associated with increased numbers of T helper cell (Th)2-type CD4+TCR-ββ (CD4+ββ) T cells producing predominantly interleukin (IL)-4. To investigate the role of these Th2-type CD4+ββ T cells, we treated TCR-α−/− mice with anti–IL-4 monoclonal antibody (mAb). Approximately 60% of TCR-α−/− mice, including those treated with mock Ab and those left untreated, spontaneously developed IBD. However, anti–IL-4 mAb–treated mice exhibited no clinical or histological signs of IBD, and their levels of mucosal and systemic Ab responses were lower than those of mock Ab–treated mice. Although TCR-α−/− mice treated with either specific or mock Ab developed CD4+ββ T cells, only those treated with anti–IL-4 mAb showed a decrease in Th2-type cytokine production at the level of mRNA and protein and an increase in interferon γ–specific expression. These findings suggest that IL-4–producing Th2-type CD4+ββ T cells play a major immunopathological role in the induction of IBD in TCR-α−/− mice, a role that anti–IL-4 mAb inhibits by causing Th2-type CD4+ββ T cells to shift to the Th1 type.

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Interfering With Inflammation: Heterogeneous Effects of Interferons in Graft-Versus-Host Disease of the Gastrointestinal Tract and Inflammatory Bowel Disease

Frontiers in Immunology ◽

10.3389/fimmu.2021.705342 ◽

2021 ◽

Vol 12 ◽

Author(s):

Eileen Haring ◽

Robert Zeiser ◽

Petya Apostolova

Keyword(s):

Inflammatory Bowel Disease ◽

Gastrointestinal Tract ◽

Graft Versus Host Disease ◽

Bowel Disease ◽

Intestinal Barrier Function ◽

Type I ◽

Hematopoietic Stem ◽

Host Disease ◽

Graft Versus Host ◽

Inflammatory Bowel

The intestine can be the target of several immunologically mediated diseases, including graft-versus-host disease (GVHD) and inflammatory bowel disease (IBD). GVHD is a life-threatening complication that occurs after allogeneic hematopoietic stem cell transplantation. Involvement of the gastrointestinal tract is associated with a particularly high mortality. GVHD development starts with the recognition of allo-antigens in the recipient by the donor immune system, which elicits immune-mediated damage of otherwise healthy tissues. IBD describes a group of immunologically mediated chronic inflammatory diseases of the intestine. Several aspects, including genetic predisposition and immune dysregulation, are responsible for the development of IBD, with Crohn’s disease and ulcerative colitis being the two most common variants. GVHD and IBD share multiple key features of their onset and development, including intestinal tissue damage and loss of intestinal barrier function. A further common feature in the pathophysiology of both diseases is the involvement of cytokines such as type I and II interferons (IFNs), amongst others. IFNs are a family of protein mediators produced as a part of the inflammatory response, typically to pathogens or malignant cells. Diverse, and partially paradoxical, effects have been described for IFNs in GVHD and IBD. This review summarizes current knowledge on the role of type I, II and III IFNs, including basic concepts and controversies about their functions in the context of GVHD and IBD. In addition, therapeutic options, research developments and remaining open questions are addressed.

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Effects of dietary tryptophan supplementation in the acetic acid-induced colitis mouse model

Food & Function ◽

10.1039/c8fo01025k ◽

2018 ◽

Vol 9 (8) ◽

pp. 4143-4152 ◽

Cited By ~ 8

Author(s):

Shuai Chen ◽

Meiwei Wang ◽

Lanmei Yin ◽

Wenkai Ren ◽

Peng Bin ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Acetic Acid ◽

Gastrointestinal Tract ◽

Mouse Model ◽

Chronic Inflammation ◽

Bowel Disease ◽

Intestinal Immunity ◽

Inflammatory Bowel

Inflammatory bowel disease (IBD) is characterized by chronic inflammation of the gastrointestinal tract and is strongly associated with intestinal immunity and the microbiome.

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Differentiation of alimentary lymphoma from inflammatory bowel disease and the influence of breed and age on the outbreak of pathological changes in the canine gastrointestinal tract

Veterinarska stanica ◽

10.46419/vs.51.3.6 ◽

2020 ◽

Vol 51 (3) ◽

pp. 293-304

Author(s):

Aneta Piplica ◽

Marko Hohšteter ◽

Lidija Medven Zagradišnik ◽

Branka Artuković ◽

Andrea Gudan Kurilj ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Gastrointestinal Tract ◽

Bowel Disease ◽

Pathological Changes ◽

Inflammatory Bowel

Cilj ovog rada bio je istražiti korisnost upotrebe imunohistokemijske metode (IHK) zajedno s histopatološkom pretragom u dijagnosticiranju i razlikovanju alimentarnog limfoma (AL) od upalne bolesti crijeva (UBC). U istraživanju su korišteni arhivski histopatološki nalazi i preparati tkiva, parafinski blokovi i stakalca. Prema pripadnosti pasmina podijelili u tri skupine malu, srednju ili veliku skupinu pasa. Cilj istraživanja bio je utvrditi povezanost pojedinih patoloških promjena ovisno o skupini pasa, dobi i spolu. Obavljenim istraživanjem zabilježili smo da je srednja vrijednost dobi u skupini malih pasmina pasa iznosila 7,58±3,59 godina, u skupini srednjih pasmina 7,45±3,04 godina te u skupini velikih pasmina 6,58±3,47 godina. Prosječna dob pasa oboljelih od alimentarnog limfoma iznosila je 8,8 godina, za razliku od upalne bolesti crijeva gdje je iznosila 7,0 godina. Unutar sve tri skupine pasa zabilježen je veći broj jedinki muškog spola. Najveći udio biopsiranih uzoraka (70,73 %) analiziran je iz tkiva tankog crijeva; po 8 uzoraka (27,59 %) iz male skupine pasa, 11 uzoraka iz srednje skupine (37,93 %) te po 10 uzoraka iz velike skupine pasa (34,48 %). Udio biopsiranog tkiva želudca bio je nešto niži (24,39 %) dok je tkivo debelog crijeva uzorkovano u samo dva psa (4,88 %). Histopatološkom analizom utvrđeno je 16 slučajeva upalne bolesti crijeva (45,71 %), 7 slučajeva limfoma (20,00 %), 9 slučajeva suspektne upale (25,71 %) te 3 slučaja suspektne upale/limfoma (8,58 %). Nakon provedene IHK metode ustanovljeno je da je u 6 slučajeva dijagnoza donesena histopatološkom evaluacijom potvrđena, u 5 slučajeva je opovrgnuta dok je u 5 slučajeva IHK metoda bila korisna kako bi razlučili AL od UBC. Utvrđene su značajne razlike (P<0,05) između dijagnoze upalne bolesti crijeva te limfoma ustanovljene patohistološkom pretragom te imunohistokemijskom metodom. Ustanovljena je češća pojavnost T staničnog limfoma u tankom crijevu dok je pojavnost B staničnog limfoma učestalija u želudcu. Povezanost s tendencijom učinka zabilježena je između patohistološke dijagnoze i CD3 biljega (r=-0.34; P=0,08) odnosno između patohistološke dijagnoze i CD79 biljega (r=0.36; P=0,09) s nešto nižom razinom povezanosti između patohistološke dijagnoze i dijagnoze IHK (r=0.21; P=0,34). Na temelju rezultata našeg istraživanja može se zaključiti da je imunohistokemijska metoda korisna za potvrdu dijagnoze i razlikovanje alimentarnog limfoma i upalne bolesti crijeva.

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Profiling of histone 3 lysine 27 acetylation reveals its role in a chronic DSS-induced colitis mouse model

Molecular Omics ◽

10.1039/c9mo00070d ◽

2019 ◽

Vol 15 (4) ◽

pp. 296-307

Author(s):

Meng Chen ◽

Qinglan Li ◽

Nan Cao ◽

Yanan Deng ◽

Lianyun Li ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Gastrointestinal Tract ◽

Mouse Model ◽

Bowel Disease ◽

Inflammatory Condition ◽

Histone 3 ◽

Chronic Inflammatory Condition ◽

Inflammatory Bowel

Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract.

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Ileocecal Burkitt’s Lymphoma Presenting as Acute Abdominal Pain

Case Reports in Oncology ◽

10.1159/000508998 ◽

2020 ◽

Vol 13 (2) ◽

pp. 968-972

Author(s):

Mostafa Mosbeh Abdelmaksoud ◽

Maram Kheder Alshareef ◽

Alaa Osama Jamjoom ◽

Mohamed Tarek Hafez

Keyword(s):

Inflammatory Bowel Disease ◽

Abdominal Pain ◽

Renal Transplantation ◽

Gastrointestinal Tract ◽

Bowel Disease ◽

Acute Abdominal Pain ◽

Emergency Laparotomy ◽

Ileocecal Region ◽

Inflammatory Bowel ◽

Hodgkin's Lymphomas

Primary gastrointestinal non-Hodgkin’s lymphomas are rare tumors which account for about 0.9% of all gastrointestinal tract tumors. They are usually associated with inflammatory bowel disease, previous radiotherapy, and renal transplantation. We report a case of non-Hodgkin’s lymphoma involving the ileocecal region in a 46-year-old gentleman who presented with acute abdominal pain that mandated emergency laparotomy.

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