Determination of serologic and colostral response in late-gestation cows vaccinated with aMycoplasma bovis bacterin

2008 ◽  
Vol 233 (1) ◽  
pp. 135-135
Author(s):  
Christopher D. Calloway ◽  
Loren G. Schultz ◽  
Munashe Chigerwe ◽  
Robert L. Larson ◽  
Robert S. Youngquist ◽  
...  
Keyword(s):  
Late Gestation

The effect of maternal nutrition on foetal serum potency in cell culture

1997 ◽  
Vol 64 (1) ◽  
pp. 111-117 ◽  
Author(s):  
M. F. Thomas ◽  
M. H. Oliver ◽  
S. C. Hodgkinson
Keyword(s):  
Cell Culture ◽  
Maternal Nutrition ◽  
Serum Insulin ◽  
Late Gestation ◽  
Cloning Efficiency ◽  
Proliferation Assay ◽  
Industry Standard ◽  
Ad Libitum ◽  
Foetal Blood

AbstractThe influence of pre-slaughter nutrition on the potency of foetal serum in cell culture was studied. Ewes carrying late-gestation foetuses (120-day gestation) were either fasted for 66 h (F), fasted for 66 h but drenched with Ketol, a propylene glycol preparation, (5 × 120 ml doses; FK), given food ad libitum (A), or given food ad libitum and drenched with Ketol (5 × 120 ml doses; AK). Following slaughter foetal blood was collected for the determination of potency in cell culture using industry-standard cell culture bioassays: cloning efficiency, plating efficiency and a 96 h cell proliferation assay. Foetal serum insulin-like growth factor (IGF) concentrations were also measured. Pre-slaughter fasting or drenching with Ketol had no effect on the potency of foetal serum in any of the cell culture bioassays. Fasting significantly lowered foetal plasma IGF-1 levels (F < 0·01). Foetal IGF-2 levels were unaffected by fasting or drenching with Ketol.

scholarly journals Determination of reference intervals for metabolic profile of Hanwoo cows at early, middle and late gestation periods

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Da Chuan Piao ◽  
Tao Wang ◽  
Jae Sung Lee ◽  
Renato SA Vega ◽  
Sang Ki Kang ◽  
...  
Keyword(s):  
Metabolic Profile ◽  
Reference Intervals ◽  
Late Gestation

scholarly journals Comparison of the properties of two forms of pyruvate kinase in rat liver and determination of their separate activities during development

1972 ◽  
Vol 127 (4) ◽  
pp. 721-731 ◽  
Author(s):  
Marshall C. Middleton ◽  
Deryck G. Walker
Keyword(s):  
Skeletal Muscle ◽  
Pyruvate Kinase ◽  
Rat Liver ◽  
Developmental Period ◽  
Late Gestation ◽  
Kinetic Properties ◽  
Foetal Rat ◽  
Neonatal Liver ◽  
Developing Rat

1. Two forms of hepatic pyruvate kinase, designated type L and type M, were distinguished on the basis of kinetic, chromatographic, electrophoretic and immunological criteria. They were partially purified and their properties compared with each other and with the purified enzyme from skeletal muscle. 2. In contrast with type L, the type M enzyme showed no marked evidence of co-operative interactions with phosphoenolpyruvate and was not stimulated by fructose diphosphate. 3. The activity profiles of type L and type M enzymes were determined in developing rat liver by utilizing differences in the kinetic properties of the two forms. The high activity of type M enzyme in the early foetal rat decreased in late gestation and immediately after birth to reach a low value, which remained essentially constant for the remainder of the developmental period. The activity of type L enzyme, in contrast, was low in the early foetal and neonatal liver but increased markedly at the onset of weaning. 4. Possible roles of the two forms of hepatic pyruvate kinase in the control of glycolysis and gluconeogenesis are discussed.

scholarly journals Evaluation of two commercially available ELISA kits for the determination of melatonin concentrations in amniotic fluid throughout pregnancy

2016 ◽  
Vol 54 (1) ◽  
pp. 107-112 ◽  
Author(s):  
Soyhan Bagci ◽  
Özlem Altuntas ◽  
David Katzer ◽  
Christoph Berg ◽  
Arne Willruth ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Early Pregnancy ◽  
Subgroup Analysis ◽  
Late Pregnancy ◽  
Late Gestation ◽  
Melatonin Concentration ◽  
Extraction Step ◽  
Significant Difference ◽  
The Mean

Background The aim of the present study is to evaluate the utility of extraction versus non-extraction-based commercial melatonin ELISA kits for determining the melatonin concentration in amniotic fluid obtained in early and late pregnancy. Methods Pregnancy duration less than 28 weeks was defined as early and from 28 weeks until delivery as late gestation. Nine samples were obtained in early and 18 in late pregnancy. Two commercially available melatonin ELISA kits (melatonin ELISA RE54021, including methanol-based extraction and direct saliva melatonin ELISA RE 54041, not including an extraction step, both from IBL-International, Germany) were used to determine melatonin concentrations in amniotic fluid. Results The mean melatonin concentration in ELISAs assayed by the non-extraction was significantly lower than those assayed after extraction. Subgroup analysis showed that there was no significant difference between melatonin concentration measured by non-extraction versus extraction ELISA in early pregnancy (11.2 ± 7.4 vs. 12.2 ± 7.7, respectively, P = 0.463) but that the mean melatonin concentration in late pregnancy was significantly lower when assayed by non-extraction ELISA than when assayed by extraction ELISA (14.8 ± 9.3 vs. 145.1 ± 179.3, respectively; P < 0.001). Agreement between both measurements in late pregnancy was rather poor (r2 = 0.271, P = 0.022), as opposed to the good correlation found in early pregnancy (r2 = 0.929, P < 0.001). Conclusions The present study revealed that a melatonin assay without an extraction step, such as direct saliva ELISA, does not seem to be a valid method to determine the melatonin concentration of amniotic fluid, especially in late gestation.

Neonatal encephalopathy: pre-clinical studies in neuroprotection

2014 ◽  
Vol 42 (2) ◽  
pp. 564-568 ◽  
Author(s):  
Shyama D. Patel ◽  
Leslie Pierce ◽  
Amber J. Ciardiello ◽  
Susan J. Vannucci
Keyword(s):  
Animal Model ◽  
Clinical Studies ◽  
Late Gestation ◽  
Therapeutic Interventions ◽  
Human Infant ◽  
Neonatal Encephalopathy ◽  
Mortality And Morbidity ◽  
Relevant Animal Model ◽  
Term Births

Neonatal encephalopathy resulting from HI (hypoxia–ischaemia) continues to be a significant cause of mortality and morbidity in infants and children, affecting 1–2/1000 live term births and up to 60% of pre-term births. In order to understand the pathophysiology of this insult, as well as design therapeutic interventions, it is important to establish a relevant animal model for pre-clinical studies. One of the most frequently used models of HI-induced brain damage in immature animals is the unilateral carotid ligation/hypoxia model, initially developed in our laboratory more than 30 years ago. The original model employed the postnatal day 7 rat, whose brain is representative of that of a late gestation, pre-term [32–36 weeks GA (gestational age)] human infant. We, and others, have employed this model to characterize the pathophysiological, biochemical/energetic and neuropathological events following HI, as well as the determination of the unique characteristics of the immature brain that define its vulnerability to, and outcome from, HI. In defining the cascade of events following HI, it has become possible to identify potential targets for intervention and neuroprotection. Currently, the only available therapeutic intervention for neonatal encephalopathy in the term asphyxiated infant is therapeutic hypothermia, although this must be initiated within 6 h of birth and is at best partially effective in moderately injured infants. Ongoing pre-clinical studies are necessary to determine the basis for the partial protection afforded by hypothermia as well as the design of adjunct therapies to improve the outcome. The present review highlights the importance of using a well-characterized and relevant animal model to continue to pursue translational research in neuroprotection for the infant brain.

Determination of GABAAα1 and GABAB1 receptor subunits expression in tissues of gilts during the late gestation

2012 ◽  
Vol 40 (2) ◽  
pp. 1377-1384 ◽  
Author(s):  
Zhiyong Fan ◽  
Yonghui Chen ◽  
Junjun Wang ◽  
Jinping Deng ◽  
Dexing Hou ◽  
...  
Keyword(s):  
Late Gestation

Determination of serologic and colostral response in late-gestation cows vaccinated with aMycoplasma bovisbacterin

2008 ◽  
Vol 69 (7) ◽  
pp. 912-915 ◽  
Author(s):  
Christopher D. Calloway ◽  
Loren G. Schultz ◽  
Munashe Chigerwe ◽  
Robert L. Larson ◽  
Robert S. Youngquist ◽  
...  
Keyword(s):  
Late Gestation

Galactic orbits of stars

1966 ◽  
Vol 25 ◽  
pp. 93-97
Author(s):  
Richard Woolley
Keyword(s):  
Globular Cluster ◽  
Potential Field ◽  
High Velocity ◽  
Velocity Vector ◽  
Variable Stars ◽  
The Sun ◽  
Proper Motions ◽  
Rr Lyrae ◽  
The Galaxy

It is now possible to determine proper motions of high-velocity objects in such a way as to obtain with some accuracy the velocity vector relevant to the Sun. If a potential field of the Galaxy is assumed, one can compute an actual orbit. A determination of the velocity of the globular clusterωCentauri has recently been completed at Greenwich, and it is found that the orbit is strongly retrograde in the Galaxy. Similar calculations may be made, though with less certainty, in the case of RR Lyrae variable stars.

Distance to SN 1987A and the LMC

1999 ◽  
Vol 190 ◽  
pp. 549-554
Author(s):  
Nino Panagia
Keyword(s):  
Angular Size ◽  
Large Magellanic Cloud ◽  
Magellanic Cloud ◽  
Light Curves ◽  
Distance Modulus ◽  
Absolute Size ◽  
Sn 1987A

Using the new reductions of the IUE light curves by Sonneborn et al. (1997) and an extensive set of HST images of SN 1987A we have repeated and improved Panagia et al. (1991) analysis to obtain a better determination of the distance to the supernova. In this way we have derived an absolute size of the ringRabs= (6.23 ± 0.08) x 1017cm and an angular sizeR″ = 808 ± 17 mas, which give a distance to the supernovad(SN1987A) = 51.4 ± 1.2 kpc and a distance modulusm–M(SN1987A) = 18.55 ± 0.05. Allowing for a displacement of SN 1987A position relative to the LMC center, the distance to the barycenter of the Large Magellanic Cloud is also estimated to bed(LMC) = 52.0±1.3 kpc, which corresponds to a distance modulus ofm–M(LMC) = 18.58±0.05.

International determination of the total motion of the pole

1961 ◽  
Vol 13 ◽  
pp. 29-41
Author(s):  
Wm. Markowitz
Keyword(s):  
Secular Motion ◽  
The Future

A symposium on the future of the International Latitude Service (I. L. S.) is to be held in Helsinki in July 1960. My report for the symposium consists of two parts. Part I, denoded (Mk I) was published [1] earlier in 1960 under the title “Latitude and Longitude, and the Secular Motion of the Pole”. Part II is the present paper, denoded (Mk II).

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