The effect of maternal nutrition on foetal serum potency in cell culture

1997 ◽  
Vol 64 (1) ◽  
pp. 111-117 ◽  
Author(s):  
M. F. Thomas ◽  
M. H. Oliver ◽  
S. C. Hodgkinson
Keyword(s):  
Cell Culture ◽  
Maternal Nutrition ◽  
Serum Insulin ◽  
Late Gestation ◽  
Cloning Efficiency ◽  
Proliferation Assay ◽  
Industry Standard ◽  
Ad Libitum ◽  
Foetal Blood

AbstractThe influence of pre-slaughter nutrition on the potency of foetal serum in cell culture was studied. Ewes carrying late-gestation foetuses (120-day gestation) were either fasted for 66 h (F), fasted for 66 h but drenched with Ketol, a propylene glycol preparation, (5 × 120 ml doses; FK), given food ad libitum (A), or given food ad libitum and drenched with Ketol (5 × 120 ml doses; AK). Following slaughter foetal blood was collected for the determination of potency in cell culture using industry-standard cell culture bioassays: cloning efficiency, plating efficiency and a 96 h cell proliferation assay. Foetal serum insulin-like growth factor (IGF) concentrations were also measured. Pre-slaughter fasting or drenching with Ketol had no effect on the potency of foetal serum in any of the cell culture bioassays. Fasting significantly lowered foetal plasma IGF-1 levels (F < 0·01). Foetal IGF-2 levels were unaffected by fasting or drenching with Ketol.

Effects of maternal nutrition during pregnancy on fetal growth and maternal constraint in sheep

2012 ◽  
Vol 52 (7) ◽  
pp. 524 ◽  
Author(s):  
C. M. C. Jenkinson ◽  
A. K. Earl ◽  
P. R. Kenyon ◽  
H. T. Blair
Keyword(s):  
Fetal Growth ◽  
Maternal Nutrition ◽  
Late Gestation ◽  
Second Trimester ◽  
Physical Constraint ◽  
Nutritional Treatment ◽  
Offspring Development ◽  
Fetal Size ◽  
Ad Libitum ◽  
Nutritional Level

This study set out to determine the stage of gestation at which maternal constraint on fetal growth occurs and whether pregnancy nutritional level could alleviate that constraint. One-hundred and thirty-eight Cheviot (C) and 114 Suffolk (S) ewes were split into two groups and bred with either 12 S or 12 C rams to generate four ewe/fetal groups CC (C dam and C sire), CSinC (crossbred fetus in C ewe), CSinS, and SS. At Day 21 of pregnancy (P21), half of the ewes in each of the four groups were randomly allocated to either a maintenance (M) or ad libitum (A) nutritional treatment, under pastoral grazing conditions. At P100, a subgroup of singleton-bearing ewes including ewes from all four groups (n = 55 in total) were euthanised (Study 1). Maternal, placental and fetal weights and sizes were recorded. The remaining ewes were fed to appetite from P140 and were allowed to lamb (n = 114 in total, Study 2) and lamb liveweights were recorded within 12 h of birth and at average days 30 and 100 (L30, L100) of lactation. In both studies, M ewes were lighter (P < 0.05) than A ewes, and CC and CSinC ewes were lighter (P < 0.05) than CSinS and SS ewes. In Study 1, maternal nutritional treatment had no effect (P > 0.05) on fetal bodyweight although fetuses from A ewes had heavier (P < 0.05) livers, spleens and thyroids than fetuses from M ewes. CC and CSinC fetuses were lighter (P < 0.01) than both CSinS and SS fetuses. In Study 2, lambs born to M ewes were lighter (P < 0.05) at birth and at L100 than lambs born to A ewes. CC lambs were lighter (P < 0.01) than CSinC, CSinS and SS lambs at birth. At L30 and L100, CC lambs were lighter (P < 0.05) than CSinC lambs, which, in turn, were lighter (P < 0.05) than both CSinS and SS lambs, which did not differ (P > 0.05). Combined, these studies indicate that maternal nutrition may have little impact on singleton-offspring development until late gestation while, in contrast, dam size affected fetal size by the end of the second trimester. These data suggest that the C ewe constrains the growth of the crossbred fetus well before a ‘physical’ constraint would be expected.

Determination of serologic and colostral response in late-gestation cows vaccinated with aMycoplasma bovis bacterin

2008 ◽  
Vol 233 (1) ◽  
pp. 135-135
Author(s):  
Christopher D. Calloway ◽  
Loren G. Schultz ◽  
Munashe Chigerwe ◽  
Robert L. Larson ◽  
Robert S. Youngquist ◽  
...  
Keyword(s):  
Late Gestation

Determination of Specific Activity of Cholera Chemical Vaccine Components using Cell Culture

2020 ◽  
Vol 36 (3) ◽  
pp. 82-89
Author(s):  
O.V. Gromova ◽  
O.S. Durakova ◽  
S.V. Generalov ◽  
L.F. Livanova ◽  
O.A. Volokh
Keyword(s):  
Cell Culture ◽  
Vibrio Cholerae ◽  
Production Control ◽  
Specific Activity ◽  
Methodological Approach ◽  
Toxin Production ◽  
Submerged Cultivation ◽  
Vaccine Production ◽  
Antigenic Activity

Том 36(2020) №3 стр. 82-89; DOI 10.21519/0234-2758-2020-36-3-82-89А.В. Гаева1*, О.В. Громова1, О.С. Дуракова1, С.В. Генералов1, Л.Ф. Ливанова1, О.А. Волох1 Определение специфической активности компонентов холерной химической вакцины с использованием культуры клеток 1ФКУЗ «Российский научно-исследовательский противочумный институт «Микроб»» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека, Саратов 410005 *[email protected] Поступила - 2019-11-26; После доработки - 2020-03-16; Принята к публикации - 2020-05-15 Список литературы Описаны методы определения динамики продукции токсинов штаммом Vibrio cholerae 569B при глубинном культивировании в биореакторе и антигенной активности специфической фракции холерогена-анатоксина по анатоксинсвязыванию с использованием клеточных культур. Показана высокая степень соответствия результатов, полученных методами, применяемыми для контроля этапов производства холерной химической вакцины и рассмотренными в данной работе. Отмечено, что применение клеточной линии СНО-К1 наиболее перспективно для замены биомоделей на промежуточных этапах контроля активных компонентов холерной химической вакцины. Разработанный методический подход впервые предлагается использовать на этапах производства холерной бивалентной химической вакцины. культура клеток, Vibrio cholerae, холерная химическая вакцина, контроль производства, холера. Vol 36(2020) N 3 p. 82-89; DOI 10.21519/0234-2758-2020-36-3-82-89A.V. Gaeva1*, O.V. Gromova1, O.S. Durakova1, S.V. Generalov1, L.F. Livanova1, O.A. Volokh1 Determination of Specific Activity of Cholera Chemical Vaccine Components using Cell Culture 1Russian Research Anti-Plague Institute «Microbe» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Saratov, 410005 *[email protected] Received - 26.11.2019; Accepted - 15.05.2020 References The methods has been described to determine the dynamics of toxin production by the Vibrio cholerae 569B strain during submerged cultivation in bioreactor and of the antigenic activity of specific choleragen anatoxin fraction by anatoxin binding levels using cell cultures. High degree of consistency was observed between the results obtained via the method under consideration and those obtained via control methods at different stages of cholera chemical vaccine production. It was shown that the CHO-K1 cell line is the most promising substitute for biomodels at the intermediate stages of control of active cholera chemical vaccine components. The developed methodological approach was first proposed for use at the stages of cholera chemical bivalent vaccine manufacturing. cell culture, Vibrio cholerae, cholera chemical vaccine, production control, cholera.

scholarly journals Determination of main parameters of ISOBUS system based agricultural machinery management

2021 ◽  
Vol 46 (3) ◽  
pp. 40-48
Author(s):  
Magó László ◽  
Kosta Gligorević ◽  
Milan Dražić ◽  
Mićo Oljača
Keyword(s):  
Communication Protocol ◽  
Plant Protection ◽  
Electronic Devices ◽  
Technological Parameters ◽  
Industry Standard ◽  
System Of Parameters ◽  
Agricultural Machines

ISO organization (https://www.iso.org) in early 90s defined an industry standard for the communication protocol among electronic devices of different manufacturers of agricultural machines. After that, all of the market actors recognised that this technology would be very important for agricultural electronics. The appearance of ISOBUS products in the market was in the mid-2000s. ISOBUS description could be found in ISO-11783 (https://www.iso.org). Through the standards and the related technical background, the production processes and the operations could be followed and monitored by the extensive Data Management. Farmers' and users legitimate needs and developing goal is to elaborate a decision support systems that follow-up the utilisation of the machines and ensure the quality of operations. For this purpose, it is essential to determine which technical, economical, technological parameters detection, measurement, transmission, processing, and evaluation becomes necessary. In our work, we reviewed which mechanical characteristic, settings are monitored within the ISOBUS system by the major machine manufacturers. We developed the system of parameters and derived features that provide effective farm-, and land-management in case of attached equipment for spreading of input materials, plant protection and tillage implements.

scholarly journals Association of Peptic Ulcer Disease with Obesity, Serum Insulin Level and Lipid Profile

2020 ◽  
Author(s):  
Foroogh Forghani ◽  
Mahboube Mirhashemi
Keyword(s):  
Peptic Ulcer ◽  
Peptic Ulcer Disease ◽  
Lipid Profile ◽  
Gastrointestinal Disease ◽  
Serum Insulin ◽  
Insulin Level ◽  
Contributing Factors ◽  
Ulcer Disease ◽  
Significant Difference

Abstract Background: PUD is a common and important type of gastrointestinal disease. Determination of contributing factors for PUD is an important issue for the development of preventive approaches. In this study, the association of Peptic Ulcer Disease with Obesity and Serum Insulin and Lipid Profile was studied.Methods: In this case-control study in Modarres Hospital in Tehran in 2019 among 170 consecutive subjects including 90 patients with PUD and 80 cases without it were enrolled and sampling tools were checklists. Serum Insulin and Lipid Profile and BMI were compared across the groupsResults: The results in this study demonstrated that Serum Insulin and Lipid Profile and BMI had no statistically significant difference between those patients with and without presence of PUD (P > 0.05).Conclusion: This study showed no statistically significant difference between those patients with and without the presence of PUD from points of Serum Insulin and Lipid Profile and BMI.

Sign in / Sign up

Export Citation Format

Share Document