Effects of Breed/Species and Gender on Platelet Concentration in Autologous Platelet Rich Plasma

Abstract Platelet rich plasma (PRP) is an autologous biological product harvested by consecutive centrifugations of whole blood and separation of plasma in a stepwise protocol. PRP has been successfully used to stimulate healing in orthopedic and dermatological conditions, both in humans and animals. The principle is the fact that α- granules inside platelets contain a high concentration of growth factors, that once released can interfere with cellular communication and speed up healing. Standardization of PRP requires establishing a gold standard for the preparation and evaluation of the product, especially considering that platelet concentration and, therefore, growth factor concentration, might vary due to a number of variables. Factors such as age, gender, race or breed, and immune status of the patient might interfere with PRP quality and with treatment results, although little is known about such interferences. This research investigated the effect of breed/species and gender in platelet concentration in autologous PRP from horses and mules. The results demonstrate that Quarter Horses provided PRP with the greatest amount of platelets, although mules had a higher concentration percentages in relation to the initial platelet counts.

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Treatment of a Refractory Skin Ulcer Using Punch Graft and Autologous Platelet-Rich Plasma

Case Reports in Dermatological Medicine ◽

10.1155/2016/7685939 ◽

2016 ◽

Vol 2016 ◽

pp. 1-4 ◽

Cited By ~ 3

Author(s):

Mauro Carducci ◽

Marcella Bozzetti ◽

Marco Spezia ◽

Giorgio Ripamonti ◽

Giuseppe Saglietti

Keyword(s):

Platelet Rich Plasma ◽

Skin Grafting ◽

Lower Limbs ◽

Alternative Approach ◽

Speed Up ◽

Autologous Platelet Rich Plasma ◽

Dermal Regeneration ◽

Diabetic Wounds ◽

Autologous Platelet ◽

Chronic Ulceration

Background. Chronic ulceration of the lower legs is a relatively common condition amongst adults: one that causes pain and social distress and results in considerable healthcare and personal costs. The technique of punch grafting offers an alternative approach to the treatment of ulcers of the lower limbs.Objective. Combining platelet-rich plasma and skin graft enhances the efficacy of treating chronic diabetic wounds by enhancing healing rate and decreasing recurrence rate. Platelet-rich plasma could, by stimulating dermal regeneration, increase the take rate after skin grafting or speed up reepithelialization.Methods and Materials. The ulcer was prepared by removing fibrin with a curette and the edges of the ulcer were freshened. The platelet-rich plasma has been infiltrated on the bottom and edges of the ulcer. The punch grafts were placed in 5 mm holes arranged. The ulcer was medicated with hydrogel and a pressure dressing was removed after 8 days.Results. After a few days the patient did not report more pain. Granulation tissue appeared quickly between implants. Most of the grafts were viable in 2-3 weeks. The grafts gradually came together to close the ulcer and were completed in four months.

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