scholarly journals Insight in Leptin Gene Polymorphism and Impact on Milk Traits in Autochtonous Busha Cattle

2019 ◽  
Vol 69 (2) ◽  
pp. 153-163 ◽  
Author(s):  
Milan Maletić ◽  
Nevres Paprikić ◽  
Miodrag Lazarević ◽  
Aida Hodžić ◽  
Vesna Davidović ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Freezing Point ◽  
Cattle Breed ◽  
Milk Traits ◽  
Native Breed ◽  
Cell Counts ◽  
Pcr Rflp ◽  
Endangered Population ◽  
High Production

Abstract Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p=0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (−0.54°C) had significantly higher (p=0.004) average FPD values than those with AB genotype (−0.58°C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.

scholarly journals PSXII-34 The evaluation of the influence of FMO3 gene polymorphism on the milk productivity of Ayrshire breed cows

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 249-250
Author(s):  
Elena Gladyr` ◽  
Elena Konovalova ◽  
Nikolay Sivkin ◽  
Olga V Kostyunina
Keyword(s):  
Gene Polymorphism ◽  
Milk Yield ◽  
Milk Protein ◽  
Cattle Breed ◽  
Monogenic Disease ◽  
World Population ◽  
Milk Productivity ◽  
Pcr Rflp ◽  
Ayrshire Breed ◽  
Significant Superiority

Abstract Russian Ayrshire population of cattle is the second largest in number the world population of animals of this breed. Trimethylaminuria (TMA) is the monogenic disease manifesting in the ‘‘fish off-flavor” of the milk from the sick cows and caused by R238X mutation in the flavin-containing monooxidase 3 gene (FMO3). The aim of our investigation was the analysis of the FMO3 gene polymorphism of Ayrshire cattle breed and its relationship with the milk productivity of the first lactation. The analysis of FMO3 gene polymorphism has been carried out on 352 animals of 5 herds, and associations with the milk productivity due 305 days have been evaluated on 20 healthy cows (RR) and 6 ones-carriers of the mutation (RX). DNA has been extracted by means of the kit “DNA-Extran” (“Syntol”, Russia). The identification of the FMO3 genotypes has been conducted by PCR-RFLP in according to the previously developed method with using TaqI restriction endonuclease. The frequency of animals-carriers of X allele associated with the defect of “fish off-flavor” of milk was in the range 10.21–12.82%. The frequencies of RR, RX and XX genotypes were 76.9, 20.5 and 2.6 %, respectively. The study of relationship between the genotypes on FMO3 gene and productivity traits has shown that the homozygous RR-individuals in comparing to RX-ones had significantly higher levels of the milk yield (+918.6 kg, P ≥ 0.05) and content of the milk protein (+28.8, P ≥ 0.05). Adaptive abilities of RR-cows of Russian selection allowed them to surpass animals of Finnish origin with a similar genotype in milk yield 5,655.6 ± 254.2 against 5,105.0 ± 238.6 kg. The investigations has shown the significant superiority the animals-non carriers of the “fish off-flavor” defect in the milk yield and content of the milk protein. This research was supported by the Ministry of science and higher education АААА-А18-118021590138-1.

scholarly journals Exploring the Genotype at CSN3 Gene, Milk Composition, Coagulation and Cheese-Yield Traits of the Sardo-Modicana, an Autochthonous Cattle Breed from the Sardinia Region, Italy

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1995
Author(s):  
Michele Pazzola ◽  
Giuseppe Massimo Vacca ◽  
Antonia Noce ◽  
Marta Porcedda ◽  
Maria Onnis ◽  
...  
Keyword(s):  
Cattle Breed ◽  
Milk Composition ◽  
Yield Traits ◽  
Milk Traits ◽  
Milk Samples ◽  
Cell Counts ◽  
Starting Point ◽  
The Mean ◽  
Extensive Farming ◽  
Milk Coagulation

The Sardo-Modicana is a local cattle breed from Sardinia, Italy. No information about its dairy potential is available in the literature. This study investigated the genotype at the CSN3 gene and milk traits of the Sardo-Modicana cattle breed. Fifty-four cows were sampled for DNA extraction and genotyping at the κ-casein gene locus, CSN3. Forty individual milk samples were analyzed for milk composition, milk coagulation properties and cheese yield (CY%). All the Sardo-Modicana cows were BB homozygotes at CSN3. Hence, the results were compared with the other two local Sardinian breeds. Eighty-three Sarda and 21 Sardo-Bruna cows were genotyped, and the A allele was found (at frequencies of 0.416 and 0.405, respectively). As regards milk traits, the mean protein value was 3.74 g/100 mL, and the mean casein value was 2.98 g/100 mL. Total bacterial and somatic cell counts showed excellent levels of hygiene considering the extensive farming and hand milking. In addition, milk produced by Sardo-Modicana cows was characterized by favorable values of coagulation properties and cheese yield. This information may represent a starting point for the conservation and enhancement of this breed.

scholarly journals Genetic polymorphism of Pit-1|HinfI gene in Grati-Ongole Grade cattle at Indonesian Beef Cattle Research Station

2018 ◽  
Vol 43 (4) ◽  
pp. 315
Author(s):  
H. Hartati ◽  
S. Anwar ◽  
B.D.P. Soewandi
Keyword(s):  
Transcription Factor ◽  
Genetic Polymorphism ◽  
Gene Polymorphism ◽  
Beef Cattle ◽  
Genomic Dna ◽  
Association Studies ◽  
Research Station ◽  
Milk Traits ◽  
Genotype Frequencies ◽  
Pcr Rflp

The pituitary transcription factor (Pit-1) gene is one of the considered genes that responsible to growth in cattle. A specific Pit-1|HinfI gene polymorphism located within exon 6(g.1256G>A) has been shown to be associated with growth and milk traits in cattle. This study aimed to identify the Pit-1|Hinf1 gene polymorphism in Grati-Ongole Grade cattle (Grati-OG cattle). A total of 107 genomic DNA of Grati-OG cattle were used in this study. The detection of polymorphism was performed by PCR-RFLP method. Only AB and BB genotypes were succesfully identified with the frequency of 0.009 and 0.991, respectively. Whereas, the frequency of A and B alleles were 0.005 and 0.995, respectively. The observed genotype frequencies in this population do not deviated from HWE. The value of Ho, He and PIC were 0.009, 0.009 and 0.009, respectively.In conclusion, the Pit-1|HinfI gene polymorphism in Grati-OG cattle is monomorphic and hence it should not be used in further association studies.

DETERMINATION OF P53 GENE CODON 72 POLYMORPHISMS IN PATIENTS WITH GASTRIC CANCER

2013 ◽  
pp. 11-17
Author(s):  
Thi Tuy Ha Nguyen ◽  
Thi Minh Thi Ha
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
P53 Gene ◽  
Diffuse Gastric Cancer ◽  
Codon 72 ◽  
Cancer Group ◽  
Pcr Rflp ◽  
Gastric Cancer Patients ◽  
The Relationship

Background: The role of p53 gene in the gastric cancer is still controversial. This study is aimed at determining the rate of the p53 gene codon 72 polymorphisms in gastric cancer patients and evaluating the relationship between these polymorphisms and endoscopic and histopathological features of gastric cancer. Patients and methods: Sixty eight patients with gastric cancer (cases) and one hundred and thirty six patients without gastric cancer (controls) were enrolled. p53 gene codon 72 polymorphisms were determined by PCR-RFLP technique with DNA extracted from samples of gastric tissue. Results: In the group of gastric cancer, Arginine/Argnine, Arginine/Proline and Proline/Proline genotypes were found in 29.4%, 42.7% and 27.9%, respectively. The differences of rates were not statistically significant between cases and controls (p > 0,05). In males, the Proline/Proline genotype was found in 38.1% in patients with gastric cancer and more frequent in patients without gastric cancer (15.7%, p = 0,01). An analysis of ROC curve showed that the cut-off was the age of 52 in the Proline/Proline genotype, but it was 65 years old in the Arginine/Proline genotype. The Proline/Proline genotype was found in 41.9% in Borrmann III/IV gastric cancer, this rate was higher than Borrmann I/II gastric cancer (16.2%, p = 0.037) and also higher than controls (18.4%, p = 0,01). The rate of Proline/Proline genotype was 41.7% in the diffuse gastric cancer, it was higher than in controls (p = 0,023). Conclusion: No significative difference of rate was found in genotypes between gastric cancer group and controls. However, there was the relationship between Proline/Proline genotype and gastric cancer in males, Borrmann types of gastric cancer, the diffuse gastric cancer. Key words: polymorphism, codon 72, p53 gene, PCR - RFLP, gastric cancer.

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