scholarly journals PSXII-34 The evaluation of the influence of FMO3 gene polymorphism on the milk productivity of Ayrshire breed cows

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 249-250
Author(s):  
Elena Gladyr` ◽  
Elena Konovalova ◽  
Nikolay Sivkin ◽  
Olga V Kostyunina
Keyword(s):  
Gene Polymorphism ◽  
Milk Yield ◽  
Milk Protein ◽  
Cattle Breed ◽  
Monogenic Disease ◽  
World Population ◽  
Milk Productivity ◽  
Pcr Rflp ◽  
Ayrshire Breed ◽  
Significant Superiority

Abstract Russian Ayrshire population of cattle is the second largest in number the world population of animals of this breed. Trimethylaminuria (TMA) is the monogenic disease manifesting in the ‘‘fish off-flavor” of the milk from the sick cows and caused by R238X mutation in the flavin-containing monooxidase 3 gene (FMO3). The aim of our investigation was the analysis of the FMO3 gene polymorphism of Ayrshire cattle breed and its relationship with the milk productivity of the first lactation. The analysis of FMO3 gene polymorphism has been carried out on 352 animals of 5 herds, and associations with the milk productivity due 305 days have been evaluated on 20 healthy cows (RR) and 6 ones-carriers of the mutation (RX). DNA has been extracted by means of the kit “DNA-Extran” (“Syntol”, Russia). The identification of the FMO3 genotypes has been conducted by PCR-RFLP in according to the previously developed method with using TaqI restriction endonuclease. The frequency of animals-carriers of X allele associated with the defect of “fish off-flavor” of milk was in the range 10.21–12.82%. The frequencies of RR, RX and XX genotypes were 76.9, 20.5 and 2.6 %, respectively. The study of relationship between the genotypes on FMO3 gene and productivity traits has shown that the homozygous RR-individuals in comparing to RX-ones had significantly higher levels of the milk yield (+918.6 kg, P ≥ 0.05) and content of the milk protein (+28.8, P ≥ 0.05). Adaptive abilities of RR-cows of Russian selection allowed them to surpass animals of Finnish origin with a similar genotype in milk yield 5,655.6 ± 254.2 against 5,105.0 ± 238.6 kg. The investigations has shown the significant superiority the animals-non carriers of the “fish off-flavor” defect in the milk yield and content of the milk protein. This research was supported by the Ministry of science and higher education АААА-А18-118021590138-1.

scholarly journals Indicators of Food Activity As Breeding Dairy Cattle Characteristics

2019 ◽  
Author(s):  
Tatyana Sedunova ◽  
Alexander Kudrin ◽  
Marina Mechanikova ◽  
Julia Smirnova
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Milk Protein ◽  
Activity Index ◽  
Effective Means ◽  
Total Activity ◽  
Milk Productivity ◽  
Ayrshire Breed ◽  
The Difference ◽  
Selection Of

The article presents the data of ethological studies on dairy cows of the Ayrshire breed of the second lactation under conditions of stall-tethered keeping in the spring. Animals with increased nutritional activity have milk yield per 305 days of lactation higher by 1662 kg (P> 0.999) compared with animals with reduced nutritional activity. The amount of milk fat in animals with increased food activity is 72.8 kg more (P> 0.999), and the total amount of milk protein is 47.8 kg more (P> 0.999) than in animals with reduced food activity. An increased index of food activity characterizes Ayrshire cows. Comparison of milk yields obtained from Ayrshire cows with milk yields of cows with reduced food activity leads to the establishment of the following results: there is a difference in milk yield for the first lactation of 1179 kg (P> 0.999), a difference in the amount of milk fat 53.2 kg (P> 0.99), and by the amount of milk protein – 36.4 kg (P> 0.99). Comparison of the total activity index led to the following results: the difference in milk yield per 305 days of lactation is 1386 kg (P> 0.999), in the amount of milk fat 61.3 kg (P> 0.999), milk protein 40.0 kg (P> 0.999). The highest correlation is observed between the index of food activity and milk productivity in ultra-active animals. In infra-passive cows, the correlation between the index of food activity and milk productivity is negative. Therefore, an effective means of increasing milk productivity is the selection of animals of Ayrshire breed by ethological individuality, taking into account indices of food, motor, and general activity. The studied indices of ethological activity are inherited by cows and can be effectively used in breeding and breeding with highly productive cattle of the Vologda selection.

scholarly journals ASSOCIATION OF POLYMORPHISM OF THE BETA-LACTOGLOBULIN GENE FROM MILK PRODUCTION OF COWS OF THE BELARUSIAN BLACK-MOTLEY BREED

2017 ◽  
Vol 53 ◽  
pp. 215-221
Author(s):  
O. A. Epishko ◽  
V. V. Peshko ◽  
N. N. Peshko
Keyword(s):  
Gene Polymorphism ◽  
Protein Content ◽  
Milk Production ◽  
Milk Fat ◽  
Milk Protein ◽  
Milk Productivity ◽  
Calculated Frequency ◽  
Pcr Rflp ◽  
Beta Lactoglobulin

In cows of the Belarusian black-motley breed using PCR-RFLP method set gene polymorphism of beta-lactoglobulin. Identified genotypes LGBAA, LGBAВ and LGBВВ. Calculated frequency of alleles and genotypes in the gene beta-lactoglobulin. Studied milk productivity (yield of milk, fat and protein content of the milk, the amount of milk fat and milk protein) in animals with different genotypes of beta-lactoglobulin. Established the superiority of the cows with genotype LGBВВ the main indicators of milk production.

Study of indexes of the suckling productivity the cows of Ayrshire breed of tribal economy of the Leningrad area.

2020 ◽  
Vol 2 ◽  
pp. 148-154
Author(s):  
Yu. V. Mukiy ◽  
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Mass Fraction ◽  
Milk Protein ◽  
Milk Productivity ◽  
Ayrshire Breed ◽  
Selection Of

In this article, studies the dynamics of indicators of the main signs of milk productivity over 305 days 7 lactations in 240 cows of the Ayrshire breed. The average values for milk yield, the mass fraction of fat and protein, the amount of milk fat and protein, the range between the maximum and minimum values were calculated and studied, and the stand-ard deviation according to the characteristics stud-ied was established. The average milk yield was higher in the 5th and 6th lactation and amounted to 8412 kg and 8962 kg; the maximum% of fat was in the 7th lactation - 4.2%, and% of the protein in the second - 3.46% and also in the last 7th lactation -3.43%. The value of the mass fraction of fat was maximum in the 1st lactation - 1.2; and the mini- mum at the 6th lactation is 0.05. The value of the mass fraction of protein was maximum in the sec-ond - 0.74 and minimum in the 7th lactation -0.24. He overall picture for these indicators represents the horizontal trend lines in the diagrams, which indicates a slight deviation of the values relative to the average for all lactations. The yield of milk fat and protein was maximum in the 6th lactation - 345.9 kg and 289.47 kg, respectively. The stand-ard deviation by milk was 568.6; by mass fraction of fat 0.09; by the mass fraction of protein 0.08; by the amount of milk fat 26.4; by the amount of milk protein 16.9; which means the uniformity of the average values for these indicators. It can be con-cluded that the selection of cattle in this complex was progressive, because led to stably high pro-duction in animals over 7 lactations.

scholarly journals Preferred and undesirable genotypes of bGH and bIGF-1 genes for the milk yield and quality of black-and-white breed

2021 ◽  
pp. 1202-1209
Author(s):  
Vadim Aleksandrovich Ulyanov ◽  
Bakhyt Zhanaidarovna Kubekova ◽  
Indira Saltanovna Beishova ◽  
Alena Valentinovna Belaya ◽  
Natalya Vladimirovna Papusha
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Milk Yield ◽  
Milk Protein ◽  
Fat Content ◽  
Milk Productivity ◽  
Black And White ◽  
Pcr Rflp ◽  
White Breed

Background and Aim: The market demand for the quality of milk and dairy products, in particular in terms of such indicators as fat content, the amount and composition of milk protein, etc., is growing. Thus, the need for the selection of dairy herds using genetic markers associated with qualitative traits of milk productivity is becoming urgent. This study aimed to determine the preferred and undesirable genotypes of the AluI polymorphism of the growth hormone gene and SnaBI polymorphism of the insulin-like growth factor-1 gene associated with milk productivity and quality indicators of the black-and-white breed. The genotypes of animals were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Materials and Methods: Samples of the black-and-white breed from Kazakhstan served as the research subjects. The polymorphism of the growth hormone and insulin-like growth factor-1 genes was determined by PCR-RFLP. The relationship of bGH-AluI and bIGF-1-SnaBI polymorphisms with productivity was assessed (fat, protein, and milk yield for 305 days of lactation, live weight, somatic cells, and milk production coefficient) by analysis of variance using Statistica 6.0 software. Results: The black-and-white cows with the bGH-AluILV genotype had significantly higher milk yield in 305 days (3174.5±157.2 kg) than those with the bGH-AluILL (2940.0±152.6 kg) and bGH-AluIVV genotypes (2964.0±36.0; p<0.05). The milk fat content of cows with genotypes bGH-AluILV and bGH-AluILL (121.8±6.5 and 120.6±10.2, respectively) was significantly higher than those with bGH-AluIVV genotype (109.8±10.8; p<0.05). The black-and-white cows with the bGH-AluILV genotype (96.7±5.3) had significantly more milk protein than those with the bGH-AluILL (90.3±5.6) and bGH-AluIVV (86.9±4.6) genotypes (p<0.05). There were no statistically significant differences in the indicators of milk productivity of cows with different genotypes of bIGF-1-SnaBI polymorphism. Conclusion: The results showed that the bGH-AluILV genotype was preferred for the black-and-white breed. The study demonstrated that genotype determined the relevant qualities, while the conditions of feeding, maintenance, and industrial technology provided the manifestation of this genotype. Thus, cows of the same (Wis Burke Ideal) line, having common ancestors in close (IV–V) ranks but living in different farming conditions, had large differences in milk productivity level. The variation was 2046 kg or 67.6% of milk per lactation.

REALIZATION OF PARENTAL INDICES OF COW PRODUCTIVITY IN DIFFERENT MILKING BREEDS AT BREEDING FARM "AGROFIRMA "PAKHMA"

2020 ◽  
Keyword(s):  
Milk Yield ◽  
Cattle Breed ◽  
Holstein Cattle ◽  
Milk Productivity ◽  
Cattle Breeds ◽  
Significant Difference ◽  
Production Indices ◽  
High Level ◽  
Beta Lactoglobulin ◽  
Percentage Protein

Combination of genotypes of main types’ genetic markers causes milk productivity in cattle. The article studies correlation between kappa casein genotypes (C3N3), beta-lactoglobulin (LGB), their complexes and milk production indices in cows bred under equal conditions at a breeding farm. The calculation of parental milk yield and fat indices between the three breeds showed a significant difference (P>0,999) of PCI of Ayrshire and Holstein cattle breed milk yield - 3395 kg, PCI of fat - 0,22%; between Holstein and Yaroslavl cattle breeds - 3200 kg, PCI offat - 0,16%. A significant difference of realization indices of protein between all three breeds was not stated. The highest realization ofparental milk yield index was stated - between Ayrshire and Yaroslavl cattle breeds- 113%, Holstein cattle breed - 88%. As for the fat and protein indices, all three cattle breeds showed a high realization of PCI from 98% to 109%. However, Holstein cattle breed showed the highest percentage: protein - 101%, fat - 109%. A significant difference of PCI realization of milk productivity in CSN3 and LGB genotypes was not stated. It was also stated that there is a tendency of high level parental indices realization when there is a higher number of B-allele variants in genotypes of cows.

scholarly journals Influence of reproductive qualities on dairy productivity of cows of the Simmental breed

2021 ◽  
Vol 285 ◽  
pp. 04005
Author(s):  
Maria Grinchuk ◽  
Yulia Nesterova
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Mass Fraction ◽  
Milk Protein ◽  
Production Performance ◽  
Dry Period ◽  
Milk Productivity ◽  
Service Period ◽  
Positive Effect ◽  
Different Levels

The aim of work is to explore influence of first fruitful insemination, service period and dry period to dairy productivity of Simmental cows. The research is aimed at studying the milk production of Simmental cows with different levels of reproductive qualities. The result of the research, a relation was established between the age of the first fruitful insemination, the duration of the service period, the duration of the dry period and milk production performance. It has been established that the age of the first fruitful insemination at 18-24 months has the most beneficial effect on subsequent milk production. The service period of 90-120 days has a positive effect to an increase in milk yield per 305 days of lactation, the longer service period negatively affects to the mass fraction of fat. With an increase in the duration of the dry period, the mass fraction of fat increases, but milk yield decreases. The influence of reproductive qualities to the milk protein is negligible. It was revealed that the age of the first insemination at 18-24 months is the most profitable for production. According to the results of the research, it can be judged that reproductive qualities, in combination with other factors, affect the milk productivity of Simmental cows.

MILK PRODUCTIVITY OF BLACK-AND-WHITE CATTLE WITH DIFFERENT GENOTYPES OF PROLACTIN AND SOMATOTROPIN

2021 ◽  
pp. 21-25
Author(s):  
Л.Р. ЗАГИДУЛЛИН ◽  
Р.Р. ШАЙДУЛЛИН ◽  
Т.М. АХМЕТОВ ◽  
С.В. ТЮЛЬКИН ◽  
А.Б. МОСКВИЧЕВА
Keyword(s):  
Protein Content ◽  
Milk Yield ◽  
Milk Fat ◽  
Milk Protein ◽  
Milk Productivity ◽  
Black And White ◽  
Gh Gene ◽  
White Cattle ◽  
Fat Yield ◽  
Prl Gene

Изучена взаимосвязь аллельных вариантов генов пролактина и соматотропина с показателями молочной продуктивности коров черно-пестрой породы по 1 и 3 лактации. У первотелок, как и у полновозрастных животных,  наиболее высокий удой (4642 и 6240 кг), количество молочного жира (174,1 и 215,0 кг) и молочного белка (149,0 и 182,5 кг) достигнуты в группе с генотипом PRL АА. По массовой доле жира и белка в молоке коровы с генотипом PRL ВВ достоверно превосходили животных с генотипом PRL АА на 0,12% (Р<0,05) и 0,05% (Р<0,05) соответственно. По гену соматотропина наибольший уровень молочной продуктивности выявлен у коров с генотипом GH LL при достоверном преимуществе над GH VV у первотелок по удою на 439 кг (Р<0,01), выходу молочного жира — на 13,6 кг (Р<0,001), выходу молочного белка — на 15,3 кг (Р<0,01). У полновозрастных коров превышение над остальными группами было только по удою — на 219—548 кг (Р<0,05). Наибольшая жирномолочность характерна животным с генотипом GH VV по 1 лактации — 3,83% и по 3 лактации — 3,82%, а лучшая белковомолочность — коровам, имеющим аллель V гена GH:  3,21 и 3,23% соответственно. Полученные данные свидетельствуют о более высоком уровне молочной продуктивности у коров с генотипом PRL АА и GH LL, но лучшая жирномолочность и белковомолочность отмечены у особей, имеющих в своем генотипе аллели В гена PRL и V гена GH. The interrelation of allelic variants of prolactin and somatotropin genes with indicators of milk productivity of black-and-white cows for the 1st and 3rd lactation was studied. The highest milk yield (4642 and 6240 kg) amount of milk fat (174.1 and 215.0 kg) and milk protein (149.0 and 182.5 kg) were in the group with the PRL AA genotype in first-calf heifers as well as in full-age animals. In terms of the mass fraction of fat and protein of milk, the cows with the PRL BB genotype significantly exceeded animals with the PRL AA genotype by 0.12% (P<0.05) and 0.05% (P<0.05), respectively. According to the somatotropin gene, the highest level of milk productivity was revealed in cows with the GH LL genotype with a significant advantage over GH VV in first-calf heifers in milk yield by 439 kg (P<0.01), milk fat yield by 13.6 kg (P<0.001), milk protein yield by 15.3 kg (P<0.01). The excess over the other groups in full-age cows was only in terms of milk yield that was by 219-548 kg (P<0.05). The highest protein content of milk is a characteristic of animals with the GH VV genotype, 3.83% is for the 1st lactation and 3.82% is for the 3rd lactation, and the best protein content of milk in cows with V allele of the GH gene is 3.21 and 3.23%, respectively. The obtained data  indicated a higher level of milk productivity in cows with the PRL AA and GH LL genotypes but the best fat and milk content was noted in cows with alleles B of the PRL gene and V of the GH gene in their genotype.

A DdeI PCR-RFLP detecting genetic variation of goat POU1F1 gene

2007 ◽  
Vol 87 (1) ◽  
pp. 13-14 ◽  
Author(s):  
X. Y. Lan ◽  
C. Y. Pan ◽  
H. Chen ◽  
C. Z. Lei
Keyword(s):  
Genetic Variation ◽  
Gene Polymorphism ◽  
Key Words ◽  
Milk Yield ◽  
Dairy Goat ◽  
Pou1f1 Gene ◽  
Pcr Rflp ◽  
Silent Allele

We described a DdeI PCR-RFLP method for detecting silent allele at goat POU1F1 locus: p.S241S. Frequencies of D1 allele varied from 0.600 to 1.000 in eight Chinese native breeds. Association of DdeI RFLP genotypes with milk yield of dairy goat was significant (P < 0.05). Key words: Goat, POU1F1 gene, polymorphism, association, milk yield

scholarly journals Insight in Leptin Gene Polymorphism and Impact on Milk Traits in Autochtonous Busha Cattle

2019 ◽  
Vol 69 (2) ◽  
pp. 153-163 ◽  
Author(s):  
Milan Maletić ◽  
Nevres Paprikić ◽  
Miodrag Lazarević ◽  
Aida Hodžić ◽  
Vesna Davidović ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Freezing Point ◽  
Cattle Breed ◽  
Milk Traits ◽  
Native Breed ◽  
Cell Counts ◽  
Pcr Rflp ◽  
Endangered Population ◽  
High Production

Abstract Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p=0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (−0.54°C) had significantly higher (p=0.004) average FPD values than those with AB genotype (−0.58°C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.

scholarly journals LEVEL OF FEEDING AND THE DYNAMICS OF MILK YIELDS OF AYRSHIRE BREED OF COWS AFTER FIRST CALVING

2021 ◽  
Vol 246 (2) ◽  
pp. 81-87
Author(s):  
O.V. Zelenina ◽  
◽  
E.V. Ermoshina ◽  
M.A. Gerasimova ◽  
◽  
...  
Keyword(s):  
Milk Yield ◽  
Dry Matter ◽  
Nutritional Value ◽  
Lactation Period ◽  
Milk Productivity ◽  
Ayrshire Breed ◽  
Digestible Protein ◽  
Daily Milk Yield ◽  
Daily Milk ◽  
Pronounced Peak

Evaluation of the nutritional value of feed mixtures for lactation periods of the Ayrshire first-calf breed showed that in the first period there is an excess of dry matter and easily digestible protein in the diet, and a lack of sugar in all periods. The consumption of energy and nutrients for the synthesis of 1 kg of milk was optimal. For 305 days of lactation, an average of 7653 kg of milk per cow was obtained after the first calving, and the maximum daily milk yield was recorded in the second month of lactation. The research of lactation curves showed that highly productive cows have a more pronounced peak of milk yield in the first lactation months; the level of milk productivity remains high up to 9-10 months of the lactation period.

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