scholarly journals Genetic polymorphism of Pit-1|HinfI gene in Grati-Ongole Grade cattle at Indonesian Beef Cattle Research Station

2018 ◽  
Vol 43 (4) ◽  
pp. 315
Author(s):  
H. Hartati ◽  
S. Anwar ◽  
B.D.P. Soewandi
Keyword(s):  
Transcription Factor ◽  
Genetic Polymorphism ◽  
Gene Polymorphism ◽  
Beef Cattle ◽  
Genomic Dna ◽  
Association Studies ◽  
Research Station ◽  
Milk Traits ◽  
Genotype Frequencies ◽  
Pcr Rflp

The pituitary transcription factor (Pit-1) gene is one of the considered genes that responsible to growth in cattle. A specific Pit-1|HinfI gene polymorphism located within exon 6(g.1256G>A) has been shown to be associated with growth and milk traits in cattle. This study aimed to identify the Pit-1|Hinf1 gene polymorphism in Grati-Ongole Grade cattle (Grati-OG cattle). A total of 107 genomic DNA of Grati-OG cattle were used in this study. The detection of polymorphism was performed by PCR-RFLP method. Only AB and BB genotypes were succesfully identified with the frequency of 0.009 and 0.991, respectively. Whereas, the frequency of A and B alleles were 0.005 and 0.995, respectively. The observed genotype frequencies in this population do not deviated from HWE. The value of Ho, He and PIC were 0.009, 0.009 and 0.009, respectively.In conclusion, the Pit-1|HinfI gene polymorphism in Grati-OG cattle is monomorphic and hence it should not be used in further association studies.

scholarly journals Insight in Leptin Gene Polymorphism and Impact on Milk Traits in Autochtonous Busha Cattle

2019 ◽  
Vol 69 (2) ◽  
pp. 153-163 ◽  
Author(s):  
Milan Maletić ◽  
Nevres Paprikić ◽  
Miodrag Lazarević ◽  
Aida Hodžić ◽  
Vesna Davidović ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Freezing Point ◽  
Cattle Breed ◽  
Milk Traits ◽  
Native Breed ◽  
Cell Counts ◽  
Pcr Rflp ◽  
Endangered Population ◽  
High Production

Abstract Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p=0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (−0.54°C) had significantly higher (p=0.004) average FPD values than those with AB genotype (−0.58°C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.

scholarly journals Polymorphism and association of progesterone receptor gene with milk production and reproductive traits of rabbits

2020 ◽  
Vol 65 (No. 9.) ◽  
pp. 346-353
Author(s):  
Lubomir Ondruska ◽  
Vladimir Parkanyi ◽  
Jan Rafay ◽  
Alica Navratilova
Keyword(s):  
Progesterone Receptor ◽  
Milk Production ◽  
Litter Size ◽  
Association Studies ◽  
Receptor Gene ◽  
Reproductive Traits ◽  
Genotype Frequencies ◽  
Progesterone Receptor Gene ◽  
Pcr Rflp ◽  
Genotype A

Using the PCR RFLP method polymorphism and three different genotypes (AA, AG and GG) were detected in the progesterone receptor gene (PGR) promoter in a local Slovak crossbred rabbit line. We have noted a slightly majority frequency of allele A (0.53) over allele G (0.47). Comparing the observed and expected genotype frequencies with the χ<sup>2</sup> test the results were statistically significant, which means the tested rabbit population was non-equilibrium. The best results and significantly highest milk production (P &lt; 0.001) were recorded in the does of GG genotype compared to AA genotype. Other association studies aimed at the effect of genotypes on litter size showed the highest litter size and number of weaned rabbits per litter in GG genotype. A significantly higher (P &lt; 0.05) average number of stillborn kits per litter was in the group of does with AA genotype (0.62) compared with GG genotype (0.34).

scholarly journals Role of engulfment and cell motility 1 (ELMO1) gene polymorphism in development of diabetic kidney disease

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Thoria A. Omar ◽  
Shimaa K. Zewain ◽  
Mohamed M. Ghonaim ◽  
Khadija A. Refaat ◽  
Dalia H. Abou-Elela
Keyword(s):  
Kidney Disease ◽  
Gene Polymorphism ◽  
Cell Motility ◽  
Diabetic Kidney Disease ◽  
Association Studies ◽  
Diabetic Patients ◽  
Genome Wide Association Studies ◽  
Diabetic Kidney ◽  
Genotype Frequencies ◽  
Significant Difference

Abstract Background Diabetic kidney disease (DKD) is a progressive kidney disease that affects diabetic patients irrespective of glycemic state or hypertension. Therefore, early detection of DKD is of critical importance. Many genome-wide association studies have identified the engulfment and cell motility 1 (ELMO1) gene as a genetic marker linked to DKD. This study aimed to investigate the association between ELMO1 rs741301 gene polymorphism and the development of DKD among Egyptian patients with type 2 diabetes mellitus (T2DM). Allele and genotype frequencies were investigated in 304 subjects by real-time PCR allelic discrimination assay: 100 DKD patients, 102 diabetic patients without DKD, and 102 healthy controls. Results GG genotype of ELMO1 (rs741301) SNP and its allele frequencies were significantly high in all diabetic patients. GG genotype had an odds ratio (OR) of 6.095 and 95% confidence interval (CI) of 2.456–15.125, p < 0.001, while the frequent allele G had an OR of 2.366 and 95% CI of 1.450–3.859, p = 0.001. No significant difference was observed between T2DM without DKD and DKD. Conclusion Our results could not establish an association between the ELMO1 rs741301 variant and the progression of DKD.

scholarly journals Genetic polymorphisms related to meat traits in purebred and crossbred Nelore cattle

2009 ◽  
Vol 44 (12) ◽  
pp. 1660-1666
Author(s):  
Rogério Abdallah Curi ◽  
Marina Rufino Salinas Fortes ◽  
Luis Artur Loyola Chardulo ◽  
Antonio Carlos Silveira ◽  
Mário De Beni Arrigoni ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Bos Taurus ◽  
Association Studies ◽  
Large Subunit ◽  
Meat Tenderness ◽  
Nucleotide Polymorphisms ◽  
Genetic Groups ◽  
Fragmentation Index ◽  
Pcr Rflp ◽  
Nelore Cattle

The objective of this work was to estimate the allelic and genotypic frequencies of CAST/XmnI, a calpastatin gene polymorphism, and CAPN530, a calpain 1 large subunit gene polymorphism, in different beef genetic groups (Nelore and Nelore x Bos taurus), and to investigate associations between these polymorphisms and carcass and meat traits. Three hundred animals - comprising 114 Nelore, 67 Angus x Nelore, 44 Rubia Gallega x Nelore, 41 Canchim, 19 Brangus three-way cross and 15 Braunvieh three-way cross- were genotyped by PCR-RFLP and phenotyped for rib-eye area (REA), back-fat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The occurrence of the two alleles of the CAST/XmnI and CAPN530 single nucleotide polymorphisms (SNPs) in a B. indicus breed, which permitted association studies in purebred and crossbred Nelore cattle, was first shown in the present work. No relationship was found between the CAST or CAPN1 SNPs and growth-related traits (REA) or fat deposition (BT and IF), since calpastatin and µ-calpain are not physiologically involved with these traits. Moreover, the association results between genotypes and aged meat tenderness (assessed by SF and MFI) showed that these markers are useless in assisted selection for purebred Nelore and their crosses with B. taurus.

scholarly journals Single Nucleotide Polymorphism (SNP) 316 on Calpain Gene in Aceh Cattle

2020 ◽  
Vol 151 ◽  
pp. 01027
Author(s):  
Teuku S. Rosa ◽  
Al Azhar ◽  
Muslim Akmal ◽  
Muhammad Hambal ◽  
Triva M. Lubis ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Meat Tenderness ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Calcium Dependent ◽  
Slaughter House ◽  
Genotype Frequencies ◽  
Pcr Rflp ◽  
Set Up ◽  
Calpain Gene

Genetic polymorphisms of μ-calpain, a calcium-dependent protease specifically expressed in muscle and related to meat tenderness, have been investigated in cattle worldwide, but not well documented in Indonesian beef cattle. This study was done to evaluate the frequency of SNP 316 in the μ-calpain gene in Aceh cattle, a local Indonesian beef cattle plays important role in the fulfillment of red meat for peoples in Aceh and its neighboring areas. For this purpose, genomic DNA was isolated from 29 sirloin meats samples collected from cull, female aceh cattle slaughtered at the Slaughter House of Banda Aceh. The cattle were purposively selected based on physical characteristics and hair colors referred to Indonesian law. For the SNP genotyping, PCR-RFLP methods were set up. The results showed all three genotypes namely CC, CG, and GG were found in Aceh cattle. The allele and genotype frequencies of SNP CAPN316 in the CAPN gene were: C – 0.14 and G – 0.86; CC – 3.4%, CG – 20.7% and GG – 75.9%. The observed and expected frequencies of CAPN SNP 316 in the cattle population examined were GG 22.0 and 21.6, CG 6.0 and 6.9, and CC 1.0 and 0.6.

scholarly journals HYPOXIA-INDUCIBLE FACTOR (HIF): STRUCTURE, FUNCTION AND GENETIC POLYMORPHISM

2019 ◽  
Vol 98 (7) ◽  
pp. 723-728 ◽  
Author(s):  
Anna G. Zhukova ◽  
A. S. Kazitskaya ◽  
T. G. Sazontova ◽  
N. N. Mikhailova
Keyword(s):  
Transcription Factor ◽  
Genetic Polymorphism ◽  
Gene Polymorphism ◽  
Adaptive Systems ◽  
Iron Homeostasis ◽  
Hypoxia Inducible Factor ◽  
The Body ◽  
Adaptive Responses ◽  
Related Factors ◽  
Expression Of Genes

Introduction. The review presents data on the structure and functions of hypoxia-inducible transcription factor - HIF. In today’s world, a person is constantly exposed to harmful damaging factors, the response of the body to which, depending on the state of adaptive systems leads either to the development of diseases or increase resistance. Important importance in the adaptation of the body to damaging effects belongs to the transcription factor, denoted as a hypoxia-inducible factor (HIF). There were identified more than 100 genes activated by HIF and therefore mediated by this transcription factor affecting the regulation of iron homeostasis, energy metabolism, the balance of Pro - and antioxidants in the cells, the activation of inhibitors of apoptosis and the formation of new blood vessels. The structure of HIF and its isoforms. The data on isoforms of HIF-α and organ-specific features of the distribution of HIF-1α, HIF-2α, and HIF-3α. Increased expression of α-subunits of transcription factor occurs in response to hypoxic effects, both acute and adaptive, psycho-emotional stress, under the action of toxic production-related factors. The increase in the level of HIF-α isoforms provides an expression of genes involved in the implementation of compensatory-adaptive responses to various harmful effects. Genetic polymorphism of the HIF. The data on the HIF-1α gene polymorphism and its association with various diseases are presented. It is shown that the most studied polymorphisms are rs11549465 C > T and rs11549467 T > C identified in the domain of oxygen-dependent degradation of the DNA sequence of the HIF-1α gene. Carriers of the C/T genotype have increased expression of HIF-1α transcription factor for rs11549465 C > T and rs11549467 T > Cpolymorphisms, Association with the risk of coronary heart disease and myocardial infarction is shown. The study of HIF-1α gene polymorphism can be promising for the diagnosis and prognosis of occupationally caused diseases, as well as the development of effective ways of their correction and prevention.

scholarly journals ABCG2 Polymorphism in Hyperuricemia associated Type 2 Diabetes and Hypertension Patients

2020 ◽  
Vol 11 (1) ◽  
pp. 1123-1129
Author(s):  
Swarnalatha J C ◽  
Amar Nagesh Kumar G ◽  
Vijaya Rachel K
Keyword(s):  
Type 2 Diabetes ◽  
Body Weight ◽  
Uric Acid ◽  
Genetic Polymorphism ◽  
Gene Polymorphism ◽  
Genomic Dna ◽  
South India ◽  
Study Population ◽  
Abcg2 Gene

Hyperuricemia is one of those syndromes that is highly inherited similar to obesity and body weight. There are many ongoing types of research in connection to the genetic inheritance of hyperuricemia. Existing studies have found out that, abnormal production of SNP rs2231142 of ABCG2gene, that belongs to the ATP-Binding cassette family, is responsible for the increased uric acid levels. Studies have proved that genetic polymorphism on the Q141K and V12M variants of the ABCG2 gene is responsible for the abnormal uric acid levels. Hence, people carrying these alleles are more likely to develop hyperuricemia. However, the extent of association of ABCG2 and hyperuricemia was found to vary with ethnicity. So, we have selected the same SNP rs 2231142 of the variants Q141K, in order to establish the theory of hyperuricemia variation with ethnicity. Here we want to study the significance of ABCG2 polymorphism in hyperuricemia patients suffering from type 2 diabetes and hypertension in the district of Srikakulam in South India. The aim of the present study was to find an association of novel ABCG2 gene with hyperuricemia in Srikakulam population attending GEMS&H, who are diagnosed as hyperuricemic and are suffering from type 2 diabetes and hypertension for more than 5 years. The study population is selected based on the disease conditions such as hypertension, hyperlipidemia, and type 2 diabetes. A total of 100 subjects were involved. Genomic DNA is extracted from the whole blood and gene polymorphism was identified by PCR and RFLP. Hyperuricemia was positively associated with age, obesity and alcoholism. The SNP locus rs2231142 of the ABCG2 gene was found to be associated with hyperuricemia and found significant in obese alcoholic men who are suffering from type 2 diabetes and hypertension.

scholarly journals Detection of GHR|AluI gene polymorphism and its association with body weight of Madura cattle in Indonesian Beef Cattle Research Station

2021 ◽  
Vol 888 (1) ◽  
pp. 012013
Author(s):  
Hartati ◽  
N H Krisna ◽  
F Firdaus ◽  
S P Rahmadani ◽  
B D P Soewandi
Keyword(s):  
Body Weight ◽  
Beef Cattle ◽  
Growth Traits ◽  
Population Sample ◽  
Growth Trait ◽  
Allele Frequencies ◽  
Research Station ◽  
Weaning Weight ◽  
Genotype Frequencies ◽  
Ghr Gene

Abstract Madura cattle is a one of the native cattle that are genetically tolerant of tropical climates. Madura cattle are one of the beef cattle breeds in Indonesia. Madura cattle can be a superior breed, but improving growth trait in Madura cattle needs to be conducted. The early step that was needed was for detecting the genes which were involved in growth traits. The purpose of this research was for detecting GHR gene polymorphisms and its association with body weight of Madura cattle population in Indonesian Beef Cattle Research Station. This study used 110 DNA samples of Madura cattle that was collected from the experimental barn at Indonesian Beef Cattle Research Station. DNA samples were extracted by DNA extraction kit. Genotype of GHR gene was detected by PCR-RFLP method using AluI restriction enzymes. In this study, association genotype and body weight was analysed by univariate GLM method. The birth weight, weaning weight, yearling weight and 18 months weight of Madura cattle were 16.8±0.3 kg; 82.4±2.3 kg; 124.6±3.7 kg and 166.7±5.0 kg, respectively. The GHR genes of Madura cattle had 3 genotypes AA, AG and GG and genotype frequencies of GHR gene were 0.273 (AA), 0.373 (AG), 0.355 (GG), respectively. Whereas the allele frequencies of A was 0.459 and the allele frequencies of G was 0.541. Based on Chi-square (X2) analysis showed that the population sample was not disequilibrium. The result of association analysis was significant (P <0.05) on weaning weight, yearling weight and 18 month weight. It was concluded that the GHR gene has potential as a genetic marker for growth traits and can be used as MAS in Madura cattle in Indonesian Beef Cattle Research Station.

scholarly journals The The favorable alleles of AKIRIN2:c.*188G>A, EDG1:c.-312A>G and TTN:g.231054C>T as candidate markers for high-marbling are very low in Bali cattle

2019 ◽  
Vol 20 (4) ◽  
pp. 965-970
Author(s):  
SAIFUL ANWAR ◽  
ARI SULISTYO WULANDARI ◽  
WIDYA PINTAKA BAYU PUTRA ◽  
SYAHRUDDIN SAID
Keyword(s):  
Genetic Polymorphism ◽  
Beef Cattle ◽  
Candidate Genes ◽  
Cattle Breed ◽  
The Other ◽  
Favorable Alleles ◽  
Factors Influencing ◽  
Pcr Rflp ◽  
Bali Cattle

Abstract. Anwar S, Wulandari AS, Putra WPB, Said S. 2019. The favorable alleles of AKIRIN2:c.*188G>A, EDG1:c.-312A>G and TTN:g.231054C>T as candidate markers for high-marbling are very low in Bali cattle. Biodiversitas 20: 965-970. Marbling is one of primary factors influencing the palatability of beef. The AKIRIN2, EDG1 and TTN genes are considered as the three candidate genes responsible for high-marbling in beef cattle. This study aimed to investigate the genetic polymorphism of the SNPs of AKIRIN2:c.*188G>A, EDG1:c.-312A>G and TTN g.231054C>T in Bali cattle. A total of 125 DNA samples of Bali cattle were genotyped for AKIRIN2 and TTN gene and 82 DNA samples for EDG1 gene using PCR-RFLP method. In the present study, it was found that the A allele of AKIRIN2:c.*188G>A, the G allele of EDG1:c.-312A>G and the T allele of TTN g.231054C>T that considered as the favorable alleles were found to be very low in Bali cattle (0.004, 0.000, and 0.004, respectively). This results indicated that the polymorphism of AKIRIN2:c.*188G>A, EDG1:c.-312A>G and TTN g.231054C>T were very low and can be considered as monomorphic in Bali cattle. These findings suggested that Bali cattle may not genetically potential as a high-marbling cattle breed. However, the other specific genes for high-marbling still needs to be investigated in Bali cattle.

Genetic polymorphism in patients with colorectal and with head and neck cancer

2009 ◽  
Vol 150 (33) ◽  
pp. 1545-1549 ◽  
Author(s):  
András Csejtei ◽  
Antal Tibold ◽  
István Ember ◽  
István Kiss
Keyword(s):  
Head And Neck Cancer ◽  
Genetic Polymorphism ◽  
Head And Neck ◽  
Neck Cancer ◽  
Pcr Rflp ◽  
Xrcc1 Arg194trp

A colorectalis rákok a második leggyakoribb halálokként szerepelnek a rosszindulatú betegségek között. A fej-nyak táji daganatok halálozása Magyarországon 265%-kal emelkedett az utóbbi 30 évben. Nem lehet eléggé hangsúlyozni e két daganatcsoport közegészségügyi jelentőségét. A colorectalis daganatok esetén a GSTM1, GSTT1 enzimek, valamint a p53 72-es kodonjának, fej-nyak táji tumorok esetén az XRCC1 Arg194Trp és Arg399Gln polimorfizmusainak hatását elemeztük. Intraoperatíve eltávolított daganatos és megfelelően illesztett daganatmentes mintákat válogattunk. A formalinban fixált mintákat deparaffinizáltuk és proteináz-K-emésztésnek vetettük alá. A genotipizálást PCR, illetve a fej-nyak táji tumorok esetén PCR-RFLP módszerrel végeztük. A vizsgált allélek gyakoriságában nem volt különbség a daganatos és a kontrollcsoport között. Túlélés tekintetében szignifikáns különbséget találtunk a GSTM1 és a p53 allélek között Dukes B stádiumú colorectalis daganatok esetén és az XRCC1 194 allélek között III-as stádiumú fej-nyak táji tumorokban. A fenti típusú genetikai különbségek szisztematikus vizsgálata a jövőben hozzájárulhat az egyéni rizikóbecslés és az individualizált kezelések megalapozásához.

Sign in / Sign up

Export Citation Format

Share Document