scholarly journals Composition of volatile compounds in heat-treated meat from polish native sheep breeds using HS-SPME-GC/MS – chemometric classification based on breed and age

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Robert Gąsior ◽  
Aldona Kawęcka ◽  
Krzysztof Wojtycza ◽  
Jacek Sikora
Keyword(s):  
Volatile Compounds ◽  
Relative Intensity ◽  
Classification Accuracy ◽  
Ratio Method ◽  
Nitrogen Compounds ◽  
Sheep Breeds ◽  
Heat Treated ◽  
Leg Muscle ◽  
Native Sheep ◽  
Sulfur And Nitrogen Compounds

AbstractIn order to characterize the volatile compounds composition of lamb meat, 3 groups of baked leg muscle samples from two Polish native sheep breeds: Świniarka aged 9 months (S9), Wrzosówka aged 9 months (W9), and Wrzosówka aged 7 months (W7), were analyzed by HS-SPME-GC/MS, followed by multivariate statistics comprising the F-Ratio method for variables pre-selection, and PCA-LDA analysis. Ninety seven volatile compounds were determined, out of which 74 were identified. The largest classes of volatile compounds were aldehydes and furans followed by alcohols, hydrocarbons, ketones, and sulfur and nitrogen compounds. Statistically significant differences between the S9, W9, and W7 groups were observed for 11 volatiles, i.e.: pentanal; hexanal; 1,3-octadiene; benzaldehyde; 3-ethyl-2-methyl-1,3-hexadiene; 3-octen-2-one; 2-octenal; 2-hexylfuran; tetradecanal; pentadecanal; hexadecanal. The HS-SPME-GC/MS method coupled with chemometrics, based on the relative intensity spectral data of these volatiles, proved to be an effective tool for the discrimination of lambs according to breed and age. The classification accuracy value for the S9, W9, and W7 groups was 100%.

scholarly journals Simultaneous Extraction of Sulfur and Nitrogen Compounds from Model Diesel Fuel Using Neoteric Green Solvents

ACS Omega ◽  
2021 ◽  
Author(s):  
Hurun E. Suhaimi ◽  
Hanee F. Hizaddin ◽  
Irfan Wazeer ◽  
Lahssen El Blidi ◽  
Mohd A. Hashim ◽  
...  
Keyword(s):  
Diesel Fuel ◽  
Nitrogen Compounds ◽  
Green Solvents ◽  
Simultaneous Extraction ◽  
Model Diesel ◽  
Sulfur And Nitrogen Compounds

Genetic variation and distances of five Italian native sheep breeds

1990 ◽  
Vol 21 (1) ◽  
pp. 87-92 ◽  
Author(s):  
M. ZANOTTI CASATI ◽  
G. C. GANDINI ◽  
P. LEONE
Keyword(s):  
Genetic Variation ◽  
Sheep Breeds ◽  
Native Sheep

scholarly journals Metabolic Availability of Lysine in Milk and a Vegetarian Cereal–Legume Meal Determined by the Indicator Amino Acid Oxidation Method in Indian Men

2020 ◽  
Vol 150 (10) ◽  
pp. 2748-2754
Author(s):  
Sulagna Bandyopadhyay ◽  
Rebecca Kuriyan ◽  
Nirupama Shivakumar ◽  
Santu Ghosh ◽  
Rajendran Ananthan ◽  
...  
Keyword(s):  
Amino Acid ◽  
Dietary Protein ◽  
Milk Powder ◽  
Cow Milk ◽  
Ratio Method ◽  
Acid Oxidation ◽  
Mixed Effect ◽  
Amino Acid Oxidation ◽  
Heat Treated ◽  
Spray Dried

ABSTRACT Background Lysine rich foods such as milk and legumes serve as important food additions to the lysine deficient cereal-based diets of vegetarian populations in low- and middle-income countries (LMICs) to alleviate the risk of quality corrected dietary protein inadequacy. Dietary protein quality can be determined by estimating the metabolic availability (MA) of lysine. Objectives The study aimed to estimate the MA of lysine in spray-dried cow milk powder (SMP), heat-treated spray-dried cow milk powder (HSMP), and a habitually consumed cereal-legume based vegetarian meal (VM), using the indicator amino acid oxidation (IAAO) slope-ratio method. Methods The MA of lysine in SMP, HSMP, and VM was estimated in 7 healthy young men aged 19–24 y with BMI of 21.5 ± 0.5 kg/m2 in a repeated measures design. The IAAO response slopes with 2 graded lysine intakes (10.5 and 15.0 mg·kg−1·d−1) from the SMP and VM were compared with the response slope generated with 3 graded crystalline lysine intakes (6.0, 10.5, and 15.0 mg·kg−1·d−1) at the subrequirement level. To produce HSMP, pasteurized cow milk was heat treated and spray dried. The MA of lysine in HSMP was tested at a single level of lysine intake (15 mg·kg−1·d−1). A total of 8 IAAO experiments were conducted on each participant in randomized order. The IAAO slopes were estimated using a linear mixed-effect regression model. Results The MA of lysine in SMP, HSMP, and VM was 91.9%, 69.9%, and 86.6% respectively. Conclusions Heat treatment reduced the MA of lysine by 22% in HSMP compared with SMP in healthy Indian adults. The lysine MA estimates can be used to optimize lysine limited cereal-based diets, with the addition of appropriately processed legumes and milk powder, to meet the protein requirement. This trial was registered at Clinical Trials Registry of India (http://ctri.nic.in) as CTRI/2019/08/020568.

Geographical isolation of native sheep breeds in the UK—Evidence of endemism as a risk factor to genetic resources

2009 ◽  
Vol 123 (2-3) ◽  
pp. 288-299 ◽  
Author(s):  
Amanda Carson ◽  
Matt Elliott ◽  
Julian Groom ◽  
Agnes Winter ◽  
Dianna Bowles
Keyword(s):  
Risk Factor ◽  
Genetic Resources ◽  
Geographical Isolation ◽  
Sheep Breeds ◽  
Native Sheep ◽  
The Uk

scholarly journals Semi-synthetic aequorins with improved sensitivity to Ca2+ ions

1989 ◽  
Vol 261 (3) ◽  
pp. 913-920 ◽  
Author(s):  
O Shimomura ◽  
B Musicki ◽  
Y Kishi
Keyword(s):  
Relative Intensity ◽  
Luminescence Intensity ◽  
Intensity Ratio ◽  
Ratio Method ◽  
Prominent Feature ◽  
Wide Range

Thirty-seven coelenterazine analogues were synthesized and incorporated into apo-aequorin, yielding 30 semi-synthetic aequorins that have the capacity to emit a significant amount of light in the presence of Ca2+. The properties of resultant photoproteins were investigated. The most prominent feature of those photoproteins was the wide range in their sensitivities to Ca2+ concentration. The relative intensity of Ca2+-triggered luminescence of the photoproteins ranged from 0.01 to 190 when compared with natural aequorin (relative intensity 1.0) at pCa 6 for the cases where the relative intensity is less than 1 and at pCa 7 for the cases where the relative intensity is higher than 1. Eight of the semi-synthetic aequorins belonged to the class of e-aequorin. With two of those photoproteins, the degree of dependence of the luminescence intensity ratio I400/I465 on pCa was greater than that with e-aequorin, suggesting that these two photoproteins are possibly superior to e-aequorin in measuring Ca2+ concentration by the ratio method.

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