scholarly journals Saliva Crystallization in Cattle: New Possibility for Early Pregnancy Diagnosis?

2013 ◽  
Vol 46 (3) ◽  
pp. 102-104 ◽  
Author(s):  
Iva Skalova ◽  
Tamara Fedorova ◽  
Karolina Brandlova
Keyword(s):  
Early Pregnancy ◽  
Artificial Insemination ◽  
Low Cost ◽  
Oestrous Cycle ◽  
Holstein Cows ◽  
Practical Application ◽  
Synchronization Process ◽  
Pregnancy Diagnosis ◽  
Non Invasive

Abstract Saliva sampling is a non-invasive, simple and low-cost procedure. The aim of this study was to confirm the presence and changes of saliva crystallization in domestic cattle during synchronized oestrous cycle and early pregnancy. We verified saliva crystallization as a method for early pregnancy diagnosis. Eight Holstein cows were included into the research. The samples were collected daily from 16th day before to 34th day after artificial insemination (in total 51 days). We observed the following types of crystallization: none, dotted, branch-like, fir-like, fern-like and combinations of them and an atypical pattern. We confirmed the presence of saliva crystallization in cattle and its changes during oestrus synchronization process, insemination and post-insemination periods. We found significant differences in pregnant and non-pregnant animals between 20th and 29th day after insemination. We concluded that pregnancy diagnosis by saliva crystallization might be possible but the practical application of this method is currently unfeasible.

scholarly journals Evaluation of synchronization protocols and methods of early pregnancy diagnosis in dairy cattle

2021 ◽  
Vol 50 (6) ◽  
Author(s):  
A.H. Shahzad ◽  
A. Sattar ◽  
N. Ahmad ◽  
I. Ahmad ◽  
M.S. Yousaf ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Early Pregnancy ◽  
Artificial Insemination ◽  
Pregnancy Loss ◽  
Holstein Cows ◽  
Plasma Progesterone ◽  
Pregnancy Diagnosis ◽  
Milk Samples ◽  
Timed Artificial Insemination ◽  
In Pregnancy

The studies aimed to evaluate the pregnancy rate (PR) for timed artificial insemination (TAI) after G7G-Ovsynch, modified G7G-Ovsynch (MG7G-Ovsynch) and Ovsynch protocols and to assess the accuracy of using pregnancy-associated glycoproteins (PAGs) and plasma progesterone (P4) in pregnancy diagnosis compared with ultrasonography (US). In study 1, Holstein cows (n = 37) were bred by TAI following the G7G-Ovsynch protocol (n = 19) or MG7G-Ovsynch (n = 18). Pregnancy was evaluated by US at days 31, 59, and 87 after breeding. The PR was not different for the G7G-Ovsynch and MG7G-Ovsynch. Blood and milk samples were collected on day 3 after insemination and then weekly through day 59 post TAI in cows diagnosed as not pregnant on day 31 and through day 87 in pregnant cows. PAGs were measured using ELISA and P4 by radioimmunoassay (RIA). In the second study, Holstein cows (n = 212) were bred by TAI following G7G-Ovsynch protocol (n = 110) or standard Ovsynch (n = 102). Cows were subjected to pregnancy diagnosis on days 30, 60, and 90. A subset (n = 15 in each group) was subjected to blood and milk samples on days 30, 45, 60, 75, and 90 to measure PAGs and P4. In study 2, PR was not significantly different between synchronization protocols on days 30, 60, and 90. Pregnancy loss averaged 15% between day 30 and day 90. The use of PAGs and P4 proved equally effective in diagnosis of pregnancy. Thus, G7G-Ovsynch was deemed the protocol of choice in postpartum cows, and PAGs assayed in milk or plasma could be used to diagnose pregnancy.

scholarly journals Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo

2020 ◽  
Vol 50 (4) ◽  
Author(s):  
Nelcio Antonio Tonizza de Carvalho ◽  
Júlia Gleyci Soares de Carvalho ◽  
José Nélio de Sousa Sales ◽  
Rodrigo Caron Macari ◽  
Pietro Sampaio Baruselli
Keyword(s):  
Statistical Analysis ◽  
Breeding Season ◽  
Artificial Insemination ◽  
Oestrous Cycle ◽  
Dominant Follicle ◽  
Induction Of Ovulation ◽  
Pregnancy Diagnosis ◽  
Timed Artificial Insemination

ABSTRACT: The aim of this study was to evaluate different times for timed artificial insemination (TAI) in buffalo submitted to a P4/E2/eCG-based protocol. In this study, 204 buffaloes were distributed into one of two groups (TAI56, n=103 and TAI64, n=101). At a random stage of the oestrous cycle (Day 0 = D0), in the morning (TAI56, a.m.) or afternoon (TAI64, p.m.), buffaloes received an intravaginal progesterone device (P4; 1.0 g) plus EB (2.0 mg i.m.). On D9 a.m. (TAI56) or p.m. (TAI64), the P4 was removed and buffaloes received PGF2a (0.53 mg i.m. sodium cloprostenol) and eCG (400 IU i.m.). On D10 a.m. (TAI56) or p.m. (TAI64), 24 h after P4 removal, buffaloes were treated with EB (1.0 mg i.m.). Buffaloes from TAI56 and TAI64 were inseminated 56 and 64 h after P4 removal (D11, p.m. and D12, a.m., respectively). Ultrasound examinations were performed on D0 to ascertain ovarian follicular status, at TAI to measure the diameter of the dominant follicle (DF) and D42 for pregnancy diagnosis. The statistical analysis was performed using the GLIMMIX procedure of SAS®. There was no difference between TAI56 and TAI64 for the diameter of the DF at TAI and the pregnancy per TAI. It was concluded that TAI 56 or 64 h after P4 removal did not affect fertility in buffaloes submitted to the induction of ovulation with EB. The present research supports that is possible to perform TAI at any time throughout the day in buffalo synchronized during the non-breeding season.

scholarly journals RESEARCHES REGARDING ESTRUS INDUCTION AND SYNCHRONIZATION IN SOWS AFTER WEANING

2009 ◽  
Vol 15 (1) ◽  
pp. 25-29
Author(s):  
Liviu BOGDAN ◽  
Ioan GROZA ◽  
Mihai CENARIU ◽  
Simona CIUPE ◽  
Emoke PALL ◽  
...  
Keyword(s):  
Economic Efficiency ◽  
Early Pregnancy ◽  
Artificial Insemination ◽  
Estrus Synchronization ◽  
Swine Farm ◽  
Pregnancy Diagnosis

The purpose of this paper was to improve the reproductive performances of sows after weaning in a private swine farm. The main objective was to implement modern reproductive biotechnologies (estrus synchronization, artificial insemination and early pregnancy diagnosis) in order to increase the economic efficiency of the reproductive sector of this farm. The biologic material used for the research was represented by 300 sows whose estrus was synchronized using three hormonal procedures (Regu-Mate administered collectively, Regu-Mate administered individually and PG600) as well as naturally, using stimulating boars. The results showed that the best methods of estrus induction and synchronization in sows use either Regu-Mate administered individually in fodder or PG600.

scholarly journals Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Reproduction ◽  
2005 ◽  
Vol 129 (3) ◽  
pp. 349-359 ◽  
Author(s):  
S Sukjumlong ◽  
A-M Dalin ◽  
L Sahlin ◽  
E Persson
Keyword(s):  
Monoclonal Antibody ◽  
Progesterone Receptor ◽  
Early Pregnancy ◽  
Artificial Insemination ◽  
Oestrous Cycle ◽  
Surface Epithelium ◽  
Physiological Changes ◽  
Receptor Proteins ◽  
High Level ◽  
Immunohistochemical Studies

Physiological changes in the sow uterus involve the regulation by progesterone and its receptor proteins (PR). Therefore, the aim of the present study was to investigate the localization of PR during different stages of the oestrous cycle and in inseminated sows during early pregnancy by use of immunohistochemistry. Uterine samples were collected from cyclic and inseminated sows at different stages of the oestrous cycle and early pregnancy. The samples were fixed in 10% formaldehyde and embedded in paraffin. Immunohistochemistry was done by use of a mouse monoclonal antibody to PR. The highest PR immunostaining in the surface epithelium was observed at oestrus/5–6 h after artificial insemination (AI) and early dioestrus/70 h after AI. In the glandular epithelium, the highest level of PR was found at oestrus with the lowest at late dioestrus/d 19. Higher levels of PR were observed in inseminated groups compared with cyclic sows. In the myometrium, a high level of PR was found at oestrus, while stromal PR cells were constantly present throughout the oestrous cycle and at different stages of early pregnancy. In conclusion, this study shows that the immunopresence of PR in the sow uterus differed between uterine compartments at the same reproductive stage. Differences were also found for some uterine compartments between cyclic and inseminated/early pregnant sows. The relatively consistent immunostaining of PR in the stroma strengthens a stromal role in the regulation of physiological activities in the sow uterus during the oestrous cycle as well as early pregnancy.

scholarly journals Non-invasive identification of protein biomarkers for early pregnancy diagnosis in the cheetah (Acinonyx jubatus)

PLoS ONE ◽  
2017 ◽  
Vol 12 (12) ◽  
pp. e0188575 ◽  
Author(s):  
Diana C. Koester ◽  
David E. Wildt ◽  
Morgan Maly ◽  
Pierre Comizzoli ◽  
Adrienne E. Crosier
Keyword(s):  
Early Pregnancy ◽  
Protein Biomarkers ◽  
Acinonyx Jubatus ◽  
Pregnancy Diagnosis ◽  
Non Invasive

scholarly journals The Foetal Ultrastructural Images Obtained from Pregnant and Non-Pregnant Does Using Transrectal and Transabdominal Probes of Ultrasound Scanner

2020 ◽  
Vol 11 (1S) ◽  
Author(s):  
Ramli Abdullah ◽  
Fatin Mardhiah Mohd
Keyword(s):  
Real Time ◽  
Early Pregnancy ◽  
Artificial Insemination ◽  
Small Ruminant ◽  
Farm Management ◽  
Gestation Period ◽  
Ultrasound Technique ◽  
Pregnancy Diagnosis ◽  
Ultrasound Scanner

Real time B-mode ultrasound scanner becomes very effective tools to detect early pregnancy diagnosis in small ruminant such as goat. Ultrasound scanner used in two way which are transrectal and transabdominal probes. Transabdominal probe used as it is less harmful than transrectal probe. The objectives of the study are: 1) to detect the ultrastructural images in non-pregnant does for artificial insemination purposes using ultrasound scanner, 2) to diagnose of difference foetal ultrastructural images obtained during gestation period using ultrasound scanner and 3) to compare the foetal ultrastructural image obtained from trans-rectal and trans-abdominal probes of ultrasound scanner. There are three pregnant does and 26 non-pregnant does with two successfully delivered out of 29 totals does chosen in this experiment. The ultrastructural images visible are ‘C’ shape placentome, heartbeat, foetus and hydrometra. It is shown that the size of heart determines the age of foetus during gestation period. At the end of this study, the farmers should be exposed to this modern way to diagnose the pregnancy and non-pregnancy in does and another ruminant. The farmer is able to manage their farm management for pregnant does and ready the non-pregnant does with culling or rebreeding for second time. The result of the study would be an alternative reference to increase the production of milk and farm management in goat industry at UDGF and Malaysia especially. The ultrasound technique to diagnose pregnancy in goats.

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