scholarly journals A new variety of Plocama calabrica (Rubiaceae) from Denizli (Turkey) confirmed by morphological and molecular ISSR markers

2019 ◽  
Vol 78 (2) ◽  
pp. 142-146
Author(s):  
Ramazan Süleyman Göktürk ◽  
Olcay Düşen ◽  
Ergun Kaya ◽  
Betül Gürcan ◽  
Uygar Sarpkaya
Keyword(s):  
Conservation Status ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
New Variety ◽  
South West ◽  
Related Variety ◽  
Distribution And Ecology ◽  
Issr Pcr ◽  
Simple Sequence ◽  
Molecular Characters

Abstract Plocama calabrica (L.f.) M.Backlund & Thulin var. alba Göktürk, O.D.Düşen, B.Gürcan & U. Sarpkaya variety nova is described from South-West Anatolia. The new variety grows on limestone slopes between Akpınar and Yaylapınar villages in the Çameli district in Denizli. It is closely related to P. calabrica var. calabrica, and can be readily distinguished by morphological and molecular characters from the related variety. Taxonomic comments such as descriptive and diagnostic characters, distribution and ecology, phenology and proposed conservation status for this new variety are given in the current study. Morphological affinities and the inter-simple-sequence repeat (ISSR)-PCR based phylogenetic relationships between the new and the related variety are also discussed

Study on the taxonomy status of alfalfa with cream flowers

2016 ◽  
Vol 39 (6) ◽  
Author(s):  
Zhiyong Li ◽  
Liu Lei ◽  
Shi Wengui ◽  
Li Hongyan ◽  
Huang Fan ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Simple Sequence Repeat ◽  
Flower Color ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Pcr Analysis ◽  
Independent Population ◽  
Taxonomical Status ◽  
Issr Pcr ◽  
Simple Sequence

Alfalfa with cream flowers is differentiable and genetically stable; however, describing it in scientific research is difficult and ambiguous because of its taxonomical status. This research attempts to delimit the taxonomical status of 14 accessions, including Medicago sativa ssp. sativa, alfalfa with cream flowers, Medicago sativa ssp. falcata and Medicago ruthinica L., using morphology, chromosome karyotypes and inter simple sequence repeat (ISSR) markers. From morphology and the ISSR-PCR analysis, three accessions of M. ruthenica L. were clustered into an independent group, and the remaining 11 accessions were clustered into three groups apparently based on flower color. Alfalfa with cream flowers had its own remarkable chromosome karyotype. The genetic distance between alfalfa with cream flowers and M. sativa ssp. sativa was almost equal to that between M. sativa ssp. falcata and M. sativa ssp. sativa. In conclusion, alfalfa with cream flowers may be an independent population just like M. sativa ssp. falcate, representing a subspecies of M. sativa ssp. according to certain taxonomic principles.

scholarly journals Genetic diversity of Panax vietnamensis var. langbianensis populations in Lam Vien plateau - Vietnam detected by inter simple sequence repeat (ISSR) markers

2020 ◽  
Vol 17 (4) ◽  
pp. 651-661
Author(s):  
Le Ngoc Trieu ◽  
Nong Van Duy ◽  
Tran Van Tien
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Sequence Repeat ◽  
New Variety ◽  
Gene Differentiation ◽  
The Stability ◽  
Simple Sequence

Panax vietnamensis var. langbianensis is a new variety from Lam Vien plateau of Vietnam. In this study, inter simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and variability of 115 individuals belonging to two naturally distributed populations of this variety, which classified by habitat. Genetic diversity at the taxon level was high (HeT = 0.284 and PPBT = 97.2 %).   The result showed lightly higher genetic diversity in population in Lac Duong region (HeLD= 0.228 and PPBLD = 81.5 %) as compared to those located in Dam Rong region (HeDR= 0.213 and PPBDR = 79.4 %). The interpopulation gene differentiation was high (GST Total = 0.221) with the genetic distance among populations was DLD-DR = 0.191. Gene flow within populations was Nm = 0.8793. In Lac Duong population, the genetic diversity of older group (HeLD O = 0.233; PPBLD O = 77.1%) was higher than of younger group (HeLD Y = 0.214; PPBLD Y = 72.4 %) and the intergroup gene differentiation was GSTDL = 0.0205 with the genetic distance between these two group was DLD O-Y  = 0.0061 showed the risk of reduction in genetic diversity. In Dam Rong population, the genetic diversity of older group (HeDR O = 0.204; PPBDR O = 75.2 %) was equal to younger group (HeDR Y = 0.209; PPBDR Y = 72.7 %) and the intergroup gene differentiation was GSTDR = 0.0304 with the genetic distance between them was DDR O-Y = 0.01393 showed the stability in genetic diversity. Data for genetic diversity and variation from this study can be used to further investigate and protect this variety for conservation and development purposes and for sustainable exploiting and use of these valuable natural resources.

Morphological and molecular determination of a new Viola species (Violaceae) from Turkey

Phytotaxa ◽  
2018 ◽  
Vol 369 (1) ◽  
pp. 37
Author(s):  
OLCAY DÜŞEN ◽  
RAMAZAN SÜLEYMAN GÖKTÜRK ◽  
ERGUN KAYA ◽  
UYGAR SARPKAYA ◽  
BETÜL GÜRCAN
Keyword(s):  
New Species ◽  
Related Species ◽  
Conservation Status ◽  
South West ◽  
Distribution And Ecology ◽  
Molecular Relationships ◽  
A New Species ◽  
Molecular Characters ◽  
Molecular Determination

Viola denizliensis is described as a new species from South-West Anatolia. The species grows on serpentinite stony slopes in Bozdağ Mountain in Denizli. It belongs to Viola sect. Melanium and is close to V. dirimliensis and V. kitaibeliana. Viola denizliensis can be readily distinguished from related species by morphological and molecular characters. Comments on descriptive and diagnostic characters, distribution and ecology, phenolgy and proposed conservation status for this new species are given in the present study. Morphological affinities and ISSR-based molecular relationships between V. denizliensis and related species are also discussed.

ISSR Marker Based Genetic Diversity in Morinda Spp. For Its Enhanced Collection, Conservation and Utilization

2021 ◽  
Author(s):  
Lalit Arya ◽  
Ramya Kossery Narayanan ◽  
Anjali Kak ◽  
Chitra Devi Pandey ◽  
Manjusha Verma ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Diversity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Morinda Citrifolia ◽  
Species Differentiation ◽  
Information Index ◽  
Upgma Cluster Analysis ◽  
Simple Sequence

Abstract Morinda (Rubiaceae) is considerably recognized for its multiple uses viz. food, medicine, dyes, firewood, tools, oil, bio-sorbent etc. The molecular characterization of such an important plant would be very useful for its multifarious enhanced utilization. In the present study, 31 Morinda genotypes belonging to two different species Morinda citrifolia and Morinda tomentosa collected from different regions of India were investigated using Inter Simple Sequence Repeat (ISSR) markers. Fifteen ISSR primers generated 176 bands with an average of 11.7 bands per primer, of which (90.34%) were polymorphic. The percentage of polymorphic bands, mean Nei’s gene diversity, mean Shannon’s information index in Morinda tomentosa and Morinda citrifolia was [(69.89%, 30.68%); (0.21 ± 0.19, 0.12 ± 0.20); (0.32 ± 0.27 0.17 ± 0.28)] respectively, revealing higher polymorphism and genetic diversity in Morinda tomentosa compared to Morinda citrifolia. Structure, and UPGMA cluster analysis placed the genotypes into well-defined separate clusters belonging to two species Morinda tomentosa and Morinda citrifolia revealing the utility of ISSR markers in species differentiation. Distinct ecotypes within a particular species could also be inferred emphasizing the collection and conservation of Morinda genotypes from different regions, in order to capture the overall diversity of respective species. Further higher diversity of M. tomentosa must be advanced for its utilization in nutraceutical, nutritional and other nonfood purposes.

scholarly journals Screening of homokaryotic protoclones of Agaricus bisporus (J. Lge) Imbach by colony characters and ISSR markers

2010 ◽  
Vol 39 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Mahmudul Islam Nazrul ◽  
Fan Xiao Lin ◽  
Bian Yin-Bing
Keyword(s):  
Simple Sequence Repeat ◽  
Agaricus Bisporus ◽  
Morphological Characters ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Fruiting Bodies ◽  
Sequence Repeat ◽  
Slow Growing ◽  
Simple Sequence ◽  
Issr Primers

Among ten slow-growing protoclones of Agaricus bisporus (J. Lge) Imbach, all appressed colonies showed slower growth rate and spawn run, and inability to produce fruiting bodies in substrate. Seven of 40 inter-simple sequence repeat (ISSR) primers amplified 78 reproducible fragments, 48.93% were polymorphic, each producing 7 to 16 bands ranging from 0.10 to 2.10 kbp, sufficient to differentiate the protoclones from each other. Appressed protoclones were homoallelic at a number of loci that were heteroallelic in the parent, suggesting that they represented rare homokaryons. Thus, using morphological characters along with ISSR, polymorphisms could be useful for quick, easy, and accurate in distinguishing homo- and heterokaryotic isolates. Key words: Agaricus bisporus (J. Lge) Imbach; Homokaryon; ISSR; Protoclone DOI: 10.3329/bjb.v39i1.5537Bangladesh J. Bot. 39(1): 119-122, 2010 (June)

Characterization of Italian populations ofLoliumspp. resistant and susceptible to diclofop by inter simple sequence repeat

Weed Science ◽  
2004 ◽  
Vol 52 (4) ◽  
pp. 554-563 ◽  
Author(s):  
Giovanni Dinelli ◽  
Alessandra Bonetti ◽  
Ilaria Marotti ◽  
Maurizio Minelli ◽  
Pietro Catizone
Keyword(s):  
Potential Role ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Intergeneric Hybridization ◽  
Banding Patterns ◽  
Inter Simple Sequence Repeats ◽  
Range Of Variation ◽  
Simple Sequence

Three ItalianLoliumweed populations, one susceptible and two resistant to diclofop, were characterized by the technique of inter simple sequence repeats (ISSR). The goal of this study was to taxonomically identify theseLoliumpopulations as well as to evaluate evidence for introgression of ISSR fragments fromFestucaand the potential role of this introgression in the diclofop response. ISSR analysis confirmed the genomic background of the weed populations to be consistent with that ofLolium. However, the great range of variation in ISSR banding patterns highlighted that the three ryegrass accessions are mixed populations made up of individuals resulting presumably from intrageneric and intergeneric hybridization in theLolium–Festucacomplex. TwoFestucagenus-discriminating and 20Festucaspecies-discriminating ISSR markers were screened among all the three ryegrass populations. The resistant Tuscania population carried the highest percentage ofFestucagenome (16.8%) followed by the resistant Roma (13.6%) and susceptible Vetralla (7.6%) populations. On the basis of these data some influence ofFestucagenome in diclofop resistance levels of studied ryegrass populations could be hypothesized.

scholarly journals Genetic diversity and interspecific relationships of some Allium species using inter simple sequence repeat markers

2015 ◽  
Vol 22 (2) ◽  
pp. 67-75 ◽  
Author(s):  
Leila Samiei ◽  
Mahnaz Kiani ◽  
Homa Zarghami ◽  
Farshid Memariani ◽  
Mohammad Reza Joharchi
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Gene Diversity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Group Method ◽  
Sequence Repeat ◽  
Allium Species ◽  
Interspecific Relationships ◽  
Simple Sequence

In this study genetic diversity and interspecific relationships of 11 Allium L. species from Khorassan province of Iran including 32 accessions were investigated by inter simple sequence repeat (ISSR) markers. Nine ISSR primers produced a total of 80 polymorphic markers and revealed high polymorphism among the studied species. The average gene diversity, effective number of alleles and Shannon’s information index were 0.2, 1.28 and 0.3, respectively. Allium kuhsorkhense exhibited the greatest level of variation (He: 0.18), whereas A. stipitatum demonstrated the lowest level of variability (He: 0.05). UPGMA (Unweighted Pair Group Method with Arithmetic mean) analysis showed that Allium accessions have a similarity range of 0.60 to 0.95. Allium scapriscapum composed the most distant group in the dendrogram. The clustered groups of Allium species clearly reflect the recent taxonomic concept of the genus at the subgenus and section levels. The present study showed that the ISSR technique is an effective molecular approach for analyzing genetic diversity and relationship in Allium species.Bangladesh J. Plant Taxon. 22(2): 67-75, 2015 (December)

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