scholarly journals Genotoxicity of chromium (III) and cobalt (II) and interactions between them

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Katarzyna Czarnek ◽  
Andrzej K. Siwicki
Keyword(s):  
Cobalt Chloride ◽  
Human Fibroblasts ◽  
The Other ◽  
Genotoxic Effects ◽  
Chromium Chloride ◽  
Essential Trace Elements ◽  
Cells With Micronuclei ◽  
Pyrimidine Bases ◽  
Ap Sites ◽  
Number Of Cells

Abstract Introduction. Chromium and cobalt are essential trace elements that are required only in a small amount, otherwise their excess can cause toxic effects. Aim. The aim of this study was to determine the effects of chromium (III) and cobalt (II) and their combinations on genotoxicity in human fibroblasts cells (BJ). Material and methods. In this work, comet and micronucleus assays were used. The BJ cells were exposed to chromium chloride and cobalt chloride at concentration ranges from 100 to 1400 µM. Mixtures of these elements were prepared so as to examine interactions between them. Results. The present study shows the genotoxic effects of chromium (III) and cobalt (II) and their mixtures on BJ cells. In the comet assay, no comets were observed at the lowest concentrations; in the higher, a significant increase in their percentage was observed. In the other assay (formation of micronuclei), a statistically significant increase in the number of cells with micronuclei was observed in the BJ cells spiked with cobalt chloride and chromium chloride. In the case of simultaneous incubation of chromium chloride at 200 µM and cobalt chloride at 1000 µM in the BJ line, antagonism was observed. However, the interaction of chromium chloride at the 1000 µM and cobalt chloride at 200 µM leads to synergism between the studied elements. Conclusions. Cobalt (II) and chromium (III) show genotoxic properties, they induce breaks in double and single-stranded DNA and they cause formation of AP-sites that do not have purine or pyrimidine bases.

The cellular structure of the female reproductive system within the Heteroderinae and Meloidogyninae (Nematoda)

Nematology ◽  
2002 ◽  
Vol 4 (8) ◽  
pp. 953-963 ◽  
Author(s):  
Etienne Geraert ◽  
Rita Van Driessche ◽  
Gerrit Karssen ◽  
Wim Bert
Keyword(s):  
Reproductive System ◽  
Cellular Structure ◽  
Cell Number ◽  
The Other ◽  
Genital System ◽  
Female Reproductive System ◽  
Young Females ◽  
Genital Structure ◽  
Number Of Cells ◽  
Female Genital

AbstractGonads from living young females, representing 23 different species, were extracted to study the cellular structure of the female genital structure within the Meloidogyninae and Heteroderinae. All genera studied can be characterised by their cellular spermatheca morphology. Within Meloidogyne a spherical spermatheca is found with lobe-like protruding cells, most species having 16 to 18 spermatheca cells with interlaced cell boundaries while M. microtyla and M. ichinohei have more spermatheca cells with different cell boundaries. Heterodera and Globodera reveal a comparable gonad structure. The spermatheca cells of Heterodera are columnar and arranged in a restricted number of rows, whereas in Globodera the spermatheca cells are squarish to rounded, depending on the species. The gonad morphology of Afenestrata koreana is clearly different from what would be expected based on the related genera Globodera and Heterodera. The apparently simplest genital system was found in Meloidodera floridensis where the uterus has a limited number of cells. In the other genera studied a large and variable cell number was found.

scholarly journals Secretion of β-N-acetylglucosaminidase isoenzymes by normal human fibroblasts

1978 ◽  
Vol 173 (2) ◽  
pp. 433-439 ◽  
Author(s):  
P Willcox
Keyword(s):  
Lysosomal Enzyme ◽  
Human Fibroblast ◽  
Lysosomal Enzymes ◽  
Culture Medium ◽  
Human Fibroblasts ◽  
The Other ◽  
Normal Human Fibroblast ◽  
Acid Hydrolases ◽  
Human Fibroblast Cultures ◽  
Normal Human

1. Secretion of the lysosomal enzyme beta-N-acetylglucosaminidase (EC 3.2.1.30) by normal human fibroblast cultures was linear with respect to time up to 96h. 2. Two forms of the A isoenzyme of beta-N-acetylglucosaminidase were found in the culture medium. One form was similar to the isoenzyme found in other extracellular fluids, such as plasma and tears, the other resembled the intracellular (lysosomal) enzyme. The presence of the two isoenzymes in the culture medium appears to reflect two distinct secretory processes. 3. It is suggested that plasma acid hydrolases may be destined for incorporation into lysosomes in a manner analogous to that described for the packaging of lysosomal enzymes by fibroblasts.

Exogenous dTMP utilization by a novel tup mutant of Saccharomyces cerevisiae

1982 ◽  
Vol 152 (1) ◽  
pp. 111-119
Author(s):  
L F Bisson ◽  
J Thorner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Culture Medium ◽  
De Novo ◽  
The Other ◽  
Normal Cells ◽  
Pyrimidine Bases

The rate and extent of entry of dTMP were measured in strains of Saccharomyces cerevisiae carrying two new tup mutations (tup5 and tup7) and most of the other tup mutations which have been reported previously by others. The tup7 mutation allowed dramatically greater accumulation of dTMP than any of the other mutations tested. Specific labeling of DNA by [CH3-3H]dTMP, fate of the dTMP pool inside of the cells, and degradation of the dTMP in the culture medium were investigated in strains carrying the tup7 mutation. The extracellular dTMP was not appreciably degraded, and that accumulated intracellularly was readily phosphorylated to dTDP and dTTP. Under optimum labeling conditions, 60 to 80% of the total thymidylate residues in newly synthesized DNA were derived from the exogenously provided dTMP, even in the absence of a block in de novo dTMP biosynthesis. An apparent Km for entry of 2 mM dTMP was found. The tup7 mutation increased permeability to dTMP (and some other 5'-mononucleotides), but did not affect uptake of nucleosides and purine and pyrimidine bases. Uptake of dTMP could be almost completely inhibited by moderate concentrations of Pi. These findings and other observations suggest that entry of dTMP in strains carrying the tup7 mutation is mediated by a permease whose function in normal cells is the transport of Pi.

Macronutrients—Nitrogen

1999 ◽  
Author(s):  
Robert F. Keefer
Keyword(s):  
Molecular Structure ◽  
Amino Acids ◽  
Adenosine Triphosphate ◽  
Plant Tissue ◽  
The Other ◽  
Biochemical Reactions ◽  
Early Seedling ◽  
Pyrimidine Bases ◽  
Coloring Matter ◽  
Chlorophyll Molecule

Important considerations concerning nitrogen in plants include the amount of nitrogen required, the forms of nitrogen (inorganic and organic) present in plant tissue, the ways that nitrogen is used in plants and affected by fertilization, and symptoms plants show when nitrogen is deficient. After the nonmineral elements, N is found in the next largest amount. More N is needed by plants than all the other nonmineral elements combined, except for K. The range of N concentrations in plants is from 0.5 to 6.0%, with most plants having 1.5 to 3.0%. Inorganic forms of N in plants are NO3- (nitrate) and NH4+ (ammonium). These forms are usually present in relatively small amounts. Other inorganic forms of N do not accumulate without injury to the plants. Organic forms of N predominate in plants, mainly as amino acids and proteins. During and after absorption, N often follows this pathway: Proteins consist of a number of amino acids linked together into a large molecular structure. Once the proteins are formed in plants, N moves to other parts of the plant only if the proteins are split apart by hydrolysis into amino acids. The amino acids then flow freely to other parts of the plant where they can recombine into proteins again. Proteins consist of 12 to 19% nitrogen. Other complex proteins formed from amino acids are enzymes that act as catalysts in biochemical reactions. Proteins also act as reserve food in the seeds that is released during germination for early seedling growth. Another type of N-containing material is chlorophyll (the green coloring matter in leaves necessary for photosynthesis). In the center of a chlorophyll molecule is a Mg atom surrounded by four N atoms. Therefore, N is a part of the chlorophyll molecule and if N is deficient, then plants become yellow since there is insufficient chlorophyll produced. Other important N-complexes are purine and pyrimidine bases that can form adenosine triphosphate (ATP) during the respiration process as an energy carrier.

8-Isocyanoamphilecta-11(20),15-diene, a new antimalarial isonitrile diterpene from the sponge Ciocalapata sp.

2009 ◽  
Vol 87 (5) ◽  
pp. 612-618 ◽  
Author(s):  
Chatchai Wattanapiromsakul ◽  
Naphatson Chanthathamrongsiri ◽  
Somchai Bussarawit ◽  
Supreeya Yuenyongsawad ◽  
Anuchit Plubrukarn ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Cell Lines ◽  
Nmr Spectra ◽  
Human Fibroblasts ◽  
Fibroblast Cell ◽  
The Other ◽  
Ergosterol Peroxide ◽  
Other Hand ◽  
Mcf 7 ◽  
Fibroblast Cell Lines

A new isonitrile diterpene of the amphilectane family, 8-isocyanoamphilecta-11(20),15-diene (4), was isolated from the sponge Ciocalapata sp., along with three known isonitriles, 8,15-diisocyano-11(20)-amphilectene (1), 7-isocyanoamphilecta-11(20),15-diene (2), and 8-isocyanoamphilecta-11(20),14-diene (3), and two steroidal peroxides, ergosterol peroxide (5) and 5α,9α-epidioxy-8α,14α-epoxy-(22E)-ergosta-6,22-dien-3β-ol (6). The structure of the new isonitrile was elucidated spectroscopically. In addition, anomalous multiplicities in the NMR spectra of some isolated isonitriles were observed and are reported here. The four isonitriles were strongly active against Plasmodium falciparum K1 with IC50 in a range of 0.09–1.07 μmol/L. Except for 1, which was cytotoxic against both MCF-7 and fibroblast cell lines, the other three diterpenes showed no significant cytotoxicity against either targeted cell lines. On the other hand, the steroidal peroxides 5 and 6, which were less active in the antimalarial bioassay (IC50 values of 6.28 and 7.13 µmol/L, respectively), were strongly cytotoxic against MCF-7 (IC50 values of 0.025 and 0.003 µmol/L, respectively), with very little toxicity against human fibroblasts.

Synthesis of 2-Deoxy-β-D-ribonucleosides and 2,3-Dideoxy-β-D-pentofuranosides on Immobilized Bacterial Cells

1994 ◽  
Vol 59 (10) ◽  
pp. 2303-2330 ◽  
Author(s):  
Ivan Votruba ◽  
Antonín Holý ◽  
Hana Dvořáková ◽  
Jaroslav Günter ◽  
Dana Hocková ◽  
...  
Keyword(s):  
The Other ◽  
Bacterial Cells ◽  
Amino Groups ◽  
E Coli ◽  
Pyrimidine Series ◽  
Acceptor Activity ◽  
Dependent Strain ◽  
Pyrimidine Bases ◽  
Heterocyclic Bases ◽  
Derivatives Of

Alginate gel-entrapped cells of auxotrophic thymine-dependent strain of E. coli catalyze the transfer of 2-deoxy-D-ribofuranosyl moiety of 2'-deoxyuridine to purine and pyrimidine bases as well as their aza and deaza analogs. All experiments invariably gave β-anomers; in most cases, the reaction was regiospecific, affording N9-isomers in the purine and N1-isomers in the pyrimidine series. Also a 2,3-dideoxynucleoside can serve as donor of the glycosyl moiety. The acceptor activity of purine bases depends only little on substitution, the only condition being the presence of N7-nitrogen atom. On the other hand, in the pyrimidine series the activity is limited to only a narrow choice of mostly short 5-alkyl and 5-halogeno uracil derivatives. Heterocyclic bases containing amino groups are deaminated; this can be avoided by conversion of the base to the corresponding N-dimethylaminomethylene derivative which is then ammonolyzed. The method was verified by isolation of 9-(2-deoxy-β-D-ribofuranosyl) derivatives of adenine, guanine, 2-chloroadenine, 6-methylpurine, 8-azaadenine, 8-azaguanine, 1-deazaadenine, 3-deazaadenine, 1-(2-deoxy-β-D-ribofuranosyl) derivatives of 5-ethyluracil, 5-fluorouracil, and 9-(2,3-dideoxy-β-D-pentofuranosyl)hypoxanthine, 9-(2,3-dideoxy-β-D-pentofuranosyl)-6-methylpurine, and other nucleosides.

The musculature of the prothoracic legs and its innervation in Hierodula membranacea (Mantidea)

1985 ◽  
Vol 309 (1140) ◽  
pp. 479-503 ◽  
Keyword(s):  
Motor Neuron ◽  
Cobalt Chloride ◽  
The Other ◽  
Single Muscle ◽  
Innervation Pattern ◽  
Sense Organs ◽  
Extensor Muscles ◽  
Praying Mantid ◽  
Orthopteroid Insects

The musculature of the fore limbs and the innervation pattern of the muscles in the praying mantid Hierodula membranacea (Burm.) are described. There are three antagonistic pairs of muscles (promotors-remotors, abductors-adductors, anterior rotator-posterior rotators) operating the prothoracic-coxal joint around three different axes. At the coxo-trochanteral, femoro-tibial and tibio-tarsal joints there are flexor and extensor muscles, but at the tarsal-pretarsal joint only flexors are present. The trochanteral extensor is a complex muscle, with both parallel-fibred and pennate parts. The trochanteral-femoral joint is operated by a single muscle, the femoral reductor. There are six pairs of prothoracic nerves, the first of which innervates the musculature of the neck and pro-mesothoracic joints. The other five nerves are all concerned with the innervation of the muscles and sense organs of the prothoracic legs. Some of the motor neuron somata in the prothorathic ganglion have been identified by using the cobalt chloride backstaining technique. The leg musculature, its innervation pattern and the location of the motor neuron somata are compared with those of other orthopteroid insects.

scholarly journals Expression and distribution of vimentin and keratin filaments in heterokaryons of human fibroblasts and amnion epithelial cells.

1982 ◽  
Vol 94 (2) ◽  
pp. 308-315 ◽  
Author(s):  
P Laurila ◽  
I Virtanen ◽  
V P Lehto ◽  
T Vartio ◽  
S Stenman
Keyword(s):  
Epithelial Cells ◽  
Intermediate Filaments ◽  
Immunofluorescence Microscopy ◽  
Human Fibroblasts ◽  
The Other ◽  
Specific Fluorescence ◽  
Keratin Filaments ◽  
Typical Cell ◽  
Vinblastine Sulphate ◽  
Amnion Epithelial Cells

The expression of intermediate filaments of the keratin- and the vimentin-type was studied in heterokaryons of human fibroblasts and amnion epithelial cells by immunofluorescence microscopy. Fibroblasts and their homokaryons showed a fibrillar, vimentin-specific fluorescence throughout the cytoplasm but were negative when stained for keratin. Amnion epithelial cells and their homokaryons, on the other hand, showed a keratin-specific fibrillar staining, and only some of them contained also detectable vimentin. When suspended epithelial cells were fused with adherent fibroblasts, keratin fibrils spread within 3 h into the fibroblasts, intermixing with the vimentin fibrils. 1-3 d after fusion, both vimentin and keratin filaments were expressed as typical fibrillar cytoplasmic arrays, and the distribution of keratin in heterokaryons resembled closely that of vimentin. A typical cell-to-cell arrangement of keratin fibrils, seen in cultures of amnion epithelial cells, could also be found between heterokaryons. Treatment of the cultures with vinblastine sulphate induced coiling of the vimentin filaments in both homo- and heterokaryons, whereas the keratin organization was only slightly affected. Our results show that both vimentin and keratin filaments are incorporated into the cytoskeleton of heterokaryons formed between fibroblasts and epithelial cells, and that they behave in the same way as in their parental cells. Both epithelial and fibroblastic characteristics thus appear to the coexpressed in such heterokaryons.

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