Nerve impulse transmission pathway-focused genes expression analysis in patients with primary hypothyroidism and autoimmune thyroiditis

AbstractObjective. Thyroid hormones have important actions in the adult brain. They regulate genes expression in myelination, differentiation of neuronal and glial cells, and neuronal viability and function.Methods. We used the pathway-specific real-time PCR array (Neurotrophins and Receptors RT2 Profiler PCR Array, QIAGEN, Germany) to identify and verify nerve impulse transmission pathway-focused genes expression in peripheral white blood cells of patients with postoperative hypothyroidism, hypothyroidism as a result of autoimmune thyroiditis (AIT) and AIT with elevated serum an anti-thyroglobulin (anti-Tg) and anti-thyroid peroxidase (anti-TPO) antibodies.Results. It was shown that patients with postoperative hypothyroidism and hypothyroidism resulting from AIT had significantly lower expression of BDNF and CBLN1. In patients with AIT with elevated serum anti-Tg and anti-TPO antibodies, the expression of GDNF was significantly down-regulated and the expression of PNOC was up-regulated. The expression levels of MEF2C and NTSR1 were decreased in the group of patients with postoperative hypothyroidism and AIT, correspondingly.Conclusions. The results of this study demonstrate that AIT and hypothyroidism can affect the expression of mRNA nerve impulse transmission genes in gene specific manner and that these changes in gene expressions can be playing a role in the development of neurological complications associated with thyroid pathology. Detection of the transcriptional activity of nerve impulse transmission genes in peripheral white blood cells can be used as an important minimally invasive prognostic marker of the risk for developing neurological complications comorbid with thyroid pathology.

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Expression of Cytokines and Cytokine Receptors-Genes in Patients with Different Forms of Thyroid Pathology in Ukrainian Population

Serbian Journal of Experimental and Clinical Research ◽

10.2478/sjecr-2021-0038 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Iryna Kamyshna ◽

Aleksandr Kamyshnyi

Keyword(s):

Autoimmune Thyroiditis ◽

Interleukin 6 ◽

Interleukin 1 ◽

White Blood Cells ◽

Elevated Serum ◽

Interleukin 10 ◽

Thyroid Peroxidase ◽

Thyroid Peroxidase Antibodies ◽

Interleukin 6 Receptor ◽

Peripheral White Blood Cells

Abstract Multiple susceptibility genes can be involved in the development of Hashimoto’s thyroiditis. Some of these genes are implicated in other autoimmune diseases, while others are specific to thyroid autoimmune response. 153 patients with thyroid pathology were enrolled in the study (152 women and 1 man, the average age was 46,02±14,3). They were divided into 3 groups: 16 patients with postoperative hypothyroidism; 65 patients with hypothyroidism resulting from autoimmune thyroiditis, and 72 patients with both AIT and elevated serum an anti-thyroglobulin and anti-thyroid peroxidase antibodies. We used a pathway-specific real-time Polymerase chain reaction array to identify and verify cytokines and receptor pathway-associated gene expression in peripheral white blood cells in randomly selected 12 individuals from each group. In the patients with postoperative hypothyroidism and those with hypothyroidism resulting from autoimmune thyroiditis, the expression of Chemokine (C-X3-C motif) receptor 1, Chemokine (C-X-C motif) receptor 4, Interleukin 6, and Interleukin 6 receptor significantly decreased, while the expression of IL6ST and IL10RA increased. In contrast, mRNA levels of Chemokine (C-X3-C motif) receptor 1, Chemokine (C-X-C motif) receptor 4, Interleukin 6, and Interleukin 6 receptor increased in the autoimmune thyroiditis patients with elevated serum anti-thyroglobulin and anti-thyroid peroxidase antibodies, while the expression of Interleukin 6 signal transducer and Interleukin 10 receptor, alpha decreased in this group of patients. The patients with hypothyroidism resulting from autoimmune thyroiditis and patients with elevated serum anti-thyroglobulin and anti-thyroid peroxidase antibodies had significantly lowered expression of Interleukin 10, while the expression of Interleukin 1, beta and Interleukin 1 receptor, type I was elevated. autoimmune thyroiditis and hypothyroidism affect the mRNA-level expression of cytokines and cytokine receptor genes in a gene-specific manner, and these changes to gene expression can be among the triggers of autoimmune inflammation progression in the thyroid gland. Transcriptional activity of cytokines, inducer, and receptor genes in the peripheral white blood cells can be used as an important minimally invasive prognostic marker of the autoimmune thyroid disease severity.

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Primary hypothyroidism and autoimmune thyroiditis alter the transcriptional activity of genes regulating neurogenesis in the blood of patients

Endocrine Regulations ◽

10.2478/enr-2021-0002 ◽

2021 ◽

Vol 55 (1) ◽

pp. 5-15

Author(s):

Iryna I. Bilous ◽

Larysa L. Pavlovych ◽

Aleksandr M. Kamyshnyi

Keyword(s):

Thyroid Hormones ◽

Autoimmune Thyroiditis ◽

Blood Cells ◽

Transcriptional Activity ◽

Primary Hypothyroidism ◽

Regulatory Genes ◽

Adult Brain ◽

Pcr Array ◽

Nervous Symptom ◽

Thyroid Pathology

Abstract Objective. Thyroid hormones play an important role in the development and maturation of the central nervous symptom and their failure in the prenatal period leading to an irreversible brain damage. Their effect on the brain of adult, however, has not been fully studied. With the discovery of neurogenesis in the adult brain, many recent studies have been focused on the understanding the basic mechanisms controlling this process. Many neurogenesis regulatory genes are not only transcribed but also translated into the blood cells. The goal of our study was to analyze the transcriptional activity of neurogenesis regulatory genes in peripheral blood cells in patients with thyroid pathology. Methods. The pathway-specific PCR array (Neurotrophins and Receptors RT2 Profiler PCR Array, QIAGEN, Germany) was used to identify and validate the neurogenesis regulatory genes expression in patients with thyroid pathology and control group. Results. The results showed that GFRA3, NGFR, NRG1, NTF3, NTRK1, and NTRK2 significantly decreased their expression in patients with autoimmune thyroiditis with rising serum of autoantibodies. The patients with primary hypothyroidism, as a result of autoimmune thyroiditis and postoperative hypothyroidism, had significantly lower expression of FGF2, NGFR, NRG1, and NTF3. The mRNA level of CNTFR was markedly decreased in the group of patients with postoperative hypothyroidism. No change in the ARTN, PSPN, TFG, MT3, and NELL1 expression was observed in any group of patients. Conclusion. The finding indicates that a decrease in thyroid hormones and a high level of autoantibodies, such as anti-thyroglobulin antibody and anti-thyroid peroxidase antibody, affect the expression of mRNA neurogenesis-regulated genes in patients with thyroid pathology.

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Apoptosis and Cell Cycle Pathway-Focused Genes Expression Analysis in Patients with Different Forms of Thyroid Pathology

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2020.4760 ◽

2020 ◽

Vol 8 (B) ◽

pp. 784-792

Author(s):

Iryna Bilous ◽

Larysa Pavlovych ◽

Inna Krynytska ◽

Mariya Marushchak ◽

Aleksandr Kamyshnyi

Keyword(s):

Cell Cycle ◽

Mrna Level ◽

Elevated Serum ◽

Thyroid Peroxidase ◽

Mrna Levels ◽

Pcr Array ◽

Thyroid Pathology ◽

Genes Expression ◽

Cell Cycle Pathway ◽

Lower Expression

BACKGROUND: Thyroid hormones are key regulators of essential cellular processes including proliferation, differentiation, and finally apoptosis. AIM: The aim of study was to detect changes in the expression of apoptosis and cell cycle pathway-focused genes in patients with different forms of thyroid pathology. PATIENTS AND METHODS: 36 patients with thyroid pathology were enrolled in the study. We used the pathway-specific real-time PCR array (Neurotrophins and Receptors RT2 Profiler PCR Array, QIAGEN, Germany) to identify and verify apoptosis and cell cycle pathway-focused genes expression in patients with postoperative hypothyroidism, hypothyroidism as a result of autoimmune thyroiditis (AIT) and AIT with elevated serum an anti-thyroglobulin (anti-Tg) and anti-thyroid peroxidase (anti-TPO) antibodies. RESULTS: It was shown that patients with elevated serum anti-Tg and anti-TPO antibodies and with hypothyroidism resulting from AIT had a significantly lower expression of FAS, TGFB, TP53, TGFA, while the expression of CD40 was increased. The mRNA levels of BCL2 and BAX decreased in the patients with elevated serum anti-Tg and anti-TPO antibodies and increased in the patients with hypothyroidism resulting from AIT and postoperative hypothyroidism. The patients with hypothyroidism resulting from AIT and postoperative hypothyroidism had significantly lower expression of HSPB1. NF1 expression did not change in all groups of patients. CONCLUSION: The results of this study demonstrate that AIT and hypothyroidism affect the mRNA-level expression of apoptosis and cell cycle pathway-focused genes in gene specific manner and that these changes to gene expression can be responsible for the apoptosis signs and symptoms associated with thyroid pathology.

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Increased serum concentrations and surface expression on peripheral white blood cells of decay-accelerating factor (cd55) in patients with active ulcerative colitis

Journal of Laboratory and Clinical Medicine ◽

10.1016/j.lab.2003.11.004 ◽

2004 ◽

Vol 143 (3) ◽

pp. 152-158 ◽

Cited By ~ 8

Author(s):

Chiho Makidono ◽

Motowo Mizuno ◽

Junichirou Nasu ◽

Sakiko Hiraoka ◽

Hiroyuki Okada ◽

...

Keyword(s):

Ulcerative Colitis ◽

Blood Cells ◽

White Blood Cells ◽

Surface Expression ◽

Active Ulcerative Colitis ◽

Serum Concentrations ◽

Decay Accelerating Factor ◽

Peripheral White Blood Cells

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The phosphatases of peripheral white blood cells in workers exposed to trichloroethylene and perchloroethylene

Occupational and Environmental Medicine ◽

10.1136/oem.26.2.159 ◽

1969 ◽

Vol 26 (2) ◽

pp. 159-161

Author(s):

A. Friborska

Keyword(s):

Blood Cells ◽

White Blood Cells ◽

Peripheral White Blood Cells

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Laparoscopic sleeve gastrectomy induces molecular changes in peripheral white blood cells

Clinical Nutrition ◽

10.1016/j.clnu.2019.03.012 ◽

2020 ◽

Vol 39 (2) ◽

pp. 592-598

Author(s):

Marc Beisani ◽

Stella Pappa ◽

Pau Moreno ◽

Eva Martínez ◽

Jordi Tarascó ◽

...

Keyword(s):

Sleeve Gastrectomy ◽

Laparoscopic Sleeve Gastrectomy ◽

Blood Cells ◽

White Blood Cells ◽

Molecular Changes ◽

Peripheral White Blood Cells

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Gene expression of the heat stress response in bovine peripheral white blood cells and milk somatic cells in vivo

Scientific Reports ◽

10.1038/s41598-020-75438-2 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

J. B. Garner ◽

A. J. Chamberlain ◽

C. Vander Jagt ◽

T. T. T. Nguyen ◽

B. A. Mason ◽

...

Keyword(s):

Gene Expression ◽

Heat Stress ◽

Blood Cells ◽

White Blood Cells ◽

Somatic Cells ◽

Holstein Friesian ◽

Milk Somatic Cells ◽

Peripheral White Blood Cells ◽

Friesian Cows

Abstract Heat stress in dairy cattle leads to reduction in feed intake and milk production as well as the induction of many physiological stress responses. The genes implicated in the response to heat stress in vivo are not well characterised. With the aim of identifying such genes, an experiment was conducted to perform differential gene expression in peripheral white blood cells and milk somatic cells in vivo in 6 Holstein Friesian cows in thermoneutral conditions and in 6 Holstein Friesian cows exposed to a short-term moderate heat challenge. RNA sequences from peripheral white blood cells and milk somatic cells were used to quantify full transcriptome gene expression. Genes commonly differentially expressed (DE) in both the peripheral white blood cells and in milk somatic cells were associated with the cellular stress response, apoptosis, oxidative stress and glucose metabolism. Genes DE in peripheral white blood cells of cows exposed to the heat challenge compared to the thermoneutral control were related to inflammation, lipid metabolism, carbohydrate metabolism and the cardiovascular system. Genes DE in milk somatic cells compared to the thermoneutral control were involved in the response to stress, thermoregulation and vasodilation. These findings provide new insights into the cellular adaptations induced during the response to short term moderate heat stress in dairy cattle and identify potential candidate genes (BDKRB1 and SNORA19) for future research.

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