scholarly journals Rapid Evaluation of Germinability of Primed China Aster (Callistephus Chinensis Ness.) Seeds with Physiological and Biochemical Markers

2014 ◽  
Vol 22 (2) ◽  
pp. 5-18 ◽  
Author(s):  
Bogumiła Badek ◽  
Zdzisława Romanowska-Duda ◽  
Mieczysław Grzesik ◽  
Bert van Duijn

AbstractThe correlation between the sowing value of primed China aster seeds represented by germination percentage (GP), mean germination time (MGT), germination uniformity expressed as the time between 25% and 75% of germinated seeds (T75-25) and some selected physiological characteristics - total activity of dehydrogenases (TAD), activity of catalase (AC), activity of cell cycle (ACC) and electrolyte leakage (EL) has been analysed in order to find useful markers of biological quality of seeds. To achieve this objective, analyses of effects of three methods of water supply to seeds viz. - hydroconditioning by soaking in excessive amount of water (M1), hydroconditioning by soaking in limited amount of water (M2) or hydroconditioning by contact with solid carrier of water - matriconditioning (M3), three levels (30.0, 35.0 and 40.0%) of seed moisture content (m.c.) and three incubation periods (1, 8 and 10 days) during priming and hence their influence on germination properties (GP, MGT, T75-25) in comparison with TAD, AC, ACC and EL were determined. The results showed that MGT and T75-25 were correlated with TAD, AC, ACC and EL, irrespective of their priming method and sowing value. Therefore, all the investigated physiological/biochemical parameters of seed quality can be used as markers of germinability and sowing value reached by primed China aster seeds. The results also proved that, irrespective of the water supply method applied, hydration of seeds up to 37.5% m.c., and their incubation at 20 °C for 8 days, followed by drying to their initial moisture content, increased to the greatest extent the speed and uniformity of seed germination and their physiological activity.

2020 ◽  
Vol 9 (9) ◽  
pp. e858998157
Author(s):  
Érica Coutinho David ◽  
Bressa Karolina Dias Cardoso ◽  
Josiene Amanda dos Santos Viana ◽  
Eniel David Cruz

Knowledge about seed storage behavior is important to preserve plant species. Clitoria fairchildiana R.A.Howard is an endemic species with medicinal properties, it is used in the recovery of degraded land. The objective of this study is to evaluate the effect of drying on the physiological quality of C. fairchildiana seeds. Seeds were oven dried along with silica gel for 18 days and in intervals of 2 or 3 days, samples were removed to assess the seed moisture content. This was done in an oven at 105±3 oC for 24h, using four replicates with 10 seeds each. After undergoing the above-mentioned treatment, a sample of seeds was stored in the freezer (-18 ºC) for 3 months. Sowing was carried out in plastic trays in a sand and sawdust mixture (1:1), previously sterilized in hot water (100 oC) for two hours. Seeds were left to germinate in a laboratory with no temperature and relative humidity control. Germination tests were performed over 14 days with daily counts of the number of emerged seedlings. The percentage of seed germination, abnormal seedlings and dead seeds were obtained 14 days after sowing. The experimental design was completely randomized with four replications of 25 seeds. The reduction of seed moisture content from 18.5% to 5.6% affected physiological seed quality causing a reduction in the germination percentage and an increase in dead seeds and abnormal seedling percentage. C. fairchildiana seeds are classified as intermediate and they can be desiccated up to 8.6% with no reduction in physiology quality.


Rice is the staple food of more than half the world’s population and is being cultivated in Asia since ancient time’s generation after generation. Good quality seeds are the seeds of the green revolution, it has been assertively and emphatically shown that 15-20% increased yield could be achieved by the use of good quality seeds alone. Good quality seed is one of the most important requirements for production and supply to achieve higher production and productivity in any crop. The seed quality is depended on various factors Viz. genetic, edaphic, environmental, biotic, etc. Good quality seed production depends on complex conditions evoking the most favorable interactions between the genetic makeup of the seed and the environment, under which it is produced, harvested, processed, and stored. Seeds undergo deterioration at various levels during storage resulting in a decline in vigor and viability. With the above considerations, the present investigation has been undertaken to observe the potentials of ten rice genotypes and observed its seed quality after harvesting in 2016-2017 at Seed Testing Laboratory, Department of Seed Science and Technology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, India. Just after harvesting G2 (Gobindabhog) and G9 (IR-36) showed highest germination percentage (97.66) and G9 (IR-36) showed highest Vigor index (3204.13). But most of the varieties germination and vigor were nearer to G2 & G9. Among the containers with seed moisture content aluminum foil packet with seed moisture content, 8% is the best storage container for rice among the other containers as up to one-year storage vigor index was observed highest in that conditions.


1987 ◽  
Vol 27 (1) ◽  
pp. 179 ◽  
Author(s):  
MA Siddique ◽  
G Somerset ◽  
PB Goodwin

Trials on the cultivars Canyon and Gallatin 50 in 1978 and Cascade in 1979 were run in North Queensland to examine ways of improving seed quality of snap beans. The trials concentrated on the maturation period, since this is a critical period for the development of seed quality. We found that seed quality was poor when the crop was cut at the stage when the leaves had fallen and all the pods were dry, or if the plants were cut at any stage and allowed to dry on the ground in single rows. This poor seed quality was associated with high pod temperatures during seed maturation. Cutting the crop before leaf fall, at a seed moisture content close to 50% (20-40% of pods dry) and windrowing immediately in 5 or 10 rows to 1 windrow gave low pod temperatures during seed maturation and high seed quality. Seed harvested and threshed directly off the crop was of good quality provided the seed moisture content in the crop had fallen to less than 25%.


Author(s):  
M Kamruzzaman ◽  
S Khatun ◽  
A Rakib ◽  
MI Hoque ◽  
MH Rani

The experiment was conducted to investigate the seed quality of Indian spinach for three months stored at different containers (Plastic pot, polythene bag and cloth bag) during 2013-2014 at Seed Technology Laboratory of Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur. The initial moisture content of the seed was 9.89% with 75.00% germination. However, after two months, the seed had a different range of increased moisture content with reduced germination percentage depending upon the packing materials. Among the three containers, seeds stored at cloth bag absorbed more moisture (14.36%) from surrounding atmosphere followed by the moisture content, 12.67% and 11.50% of polythene bag and airtight plastic pot, respectively. Seeds stored at plastic pot, polythene bag and cloth bag exhibited 65.00, 64.00 and 50.00 per cent germination, respectively. Similarly, the fresh weight and dry weight of seedling and seedling vigor were also decreased. The seeds of plastic pot were good in term of germination capacity, fresh and dry weight and vigor index in comparison to those stored at cloth bag and polythene bag.Int. J. Agril. Res. Innov. & Tech. 5 (2): 51-57, December, 2015


2014 ◽  
Vol 24 (2) ◽  
pp. 177-180 ◽  
Author(s):  
Todd P. West ◽  
Samuel L. DeMarais ◽  
Chiwon W. Lee

Temperate-zone woody plant species generally require seed stratification to overcome embryo dormancy. Embryo dormancy is variable in japanese tree lilac (Syringa reticulata) with a recommendation of 30- to 90-days stratification at 1–5 °C. Cultivar propagation generally is done by grafting onto seedling rootstocks. It would be advantageous to rootstock seedling production to be able to reduce or eliminate the need for seed stratification to increase germination numbers as well as having production moved from field beds to greenhouses for quicker production of high-quality rootstock plants suitable for grafting. Research objective of this study was to determine if “green” seed could be used without the need of stratification for japanese tree lilac seedling production. Seed capsule fresh weight and seed moisture content were evaluated to determine if these factors could be used as predictors of germination percentages. Seed was randomly collected at the North Dakota State University campus in Fargo, ND, for seven consecutive weeks starting in Sept. 2011 and 2012. Germination and seed moisture tests were performed weekly. Germination percentage was highest (89.5%) at week 2 and steadily decreased to 0% at week 7. Germination percentages were 77.5%, 89.5%, 78.5%, 67%, 24.5%, 1.5%, and 0% for consecutive collection weeks 1–7, respectively. Seed moisture content was 59.0%, 52.6%, 49.8%, 51.8%, 44.5%, 27.4%, and 8.6% for collection weeks 1–7, respectively. Germination percentage was directly correlated with seed moisture content and decreased as seed capsules matured (natural drying and splitting of capsule seem to disperse seed) during the fall season. Data suggest that timing of fall seed collection from japanese tree lilac is critical and must be done before maturation of the seed capsule to avoid the stratification requirement. Seed capsules with an average fresh weight higher than 0.2 g and seed moisture content greater than 50% produced the highest germination rates without requiring stratification.


2007 ◽  
Vol 43 (3) ◽  
pp. 301-317 ◽  
Author(s):  
R. K. BAM ◽  
P. Q. CRAUFURD ◽  
P. T. DORWARD ◽  
E. A. ASIEDU ◽  
F. K. KUMAGA ◽  
...  

Improved upland rice cultivars introduced in Volta Region, Ghana, have been perceived to store poorly compared to farmers' traditional cultivars. A survey was conducted in 2003 in the Hohoe district of this region, where a Participatory Varietal Selection programme had started in 1997, to gain insight into farmers' seed production and storage practices that are likely to affect seed quality in storage. Farmers rated keeping quality (p < 0.001), tolerance to storage pests (p < 0.001), seed quality (p < 0.001) and establishment of their local cultivars Kawomo, Viono and Wuwulili as much better than the improved cultivar IDSA 85. Initial seed moisture content ranged from 12.8 to 18% and germination from 0 to 82%. There was a significant relationship between seed moisture content and duration of drying prior to storage (p < 0.001) and storage method (p = 0.015). Germination loss in storage was rapid at high moisture content and slow at low moisture content. Between 60 and 80% of seeds germinated after six months storage at 12.8% moisture content. The viability equation predicted accurately germination of farmer-saved seed stored under ambient temperature in Ghana. Except for the japonica rice cultivar WAB 126-18-HB, the traditional cultivars Kawomo, Viono and Wuwulili survived better in storage than improved cultivars. There is a need to improve seed quality of improved cultivars if farmers are to benefit from their higher yields and grain quality and to improve storage practices.


2019 ◽  
Vol 11 (4) ◽  
pp. 352-355
Author(s):  
N. Khatri ◽  
D. Pokhrel ◽  
B.P. Pandey ◽  
K.R. Pant ◽  
M. Bista

Abstract. A study was conducted at four Village Development Committees (Masuriya, Udashipur, Basauti and Pahalmanpur) of Kailali dis- trict, Far western Region, Nepal in 2016/17 to find out the effect of different storage materials on the seed moisture content and germination percentage of wheat. Seeds of wheat were packed using five storage materials (metal seed bin, earthen pot, purdue improved crop storage / Pics/ bag, gunny bag and super grain bag). The stored seed samples were drawn at three-month intervals starting from May 2016 to January 2017. The experiment was arranged in Randomized Complete Block Design (RCBD) in four replications. The results of analysis of variance revealed that the effect of storage materials was significant (P≤0.05) on seed moisture content and seed germination of wheat. After nine months of storage, the seeds stored in Pics bag recorded the lowest moisture (13.3%) and temperature (26.4°C), and the highest germination percentage (91.75%). The lowest quality performance (high moisture and temperature, low germination) of stored seeds was observed in an earthen pot. From the results obtained, it was concluded that wheat seed could be stored for a long time in Pics bag without deteriorating its quality parameters.


2017 ◽  
Vol 9 (1) ◽  
pp. 245-252
Author(s):  
J. B. Patel ◽  
Jyoti Sondarva ◽  
C. A. Babariya ◽  
R. R. Rathod ◽  
V. J. Bhatiya

The present investigation was carried out in laboratory of the Department of Seed Science and Technolo-gy, College of Agriculture, Junagadh Agricultural University, Junagadh from the April 2013 to April 2015, wherein two kg of freshly harvested quality seed of soybean cv. Gujarat Junagadh Soybean 3 having high germination percentage and low moisture content (below 8%) was taken for each repetition and for each combination of treat-ments. The treatment consisted of two storage conditions (C) viz., C1 (Ambient temperature) and C2 (Cold storage at 7oC + 2oC), and five seed treatments (S) viz., S1 = Control, S2 = Carbendazim @ 2g/kg seed, S3 = Mancozeb @ 2g/ kg seed, S4 = Neem leaf powder @ 10g/kg seed, and S5 = Neem Oil @ 5 ml/kg seed. The experiment was carried out using Completely Randomized Design (Factorial) repeated three times. After proper mixing or smearing the seeds as per the treatments, seeds were packed in cloth bag and kept in laboratory under two different storage conditions. Observations were recorded at 90 days interval on germination (%), root length (cm), shoot length (cm), seedling dry weight (g), seed vigour index I, seed vigour index II and seed moisture content (%). The results revealed that storage condition (C) and seed treatments (S) exhibited significant differences almost for the all the traits for germination and seedling parameters after 2 years of storage. The results of soybean seed stored in two different storage conditions showed that on an average, the seed stored under cold storage (70C + 20C) noted higher values for all the traits studied except seed moisture content after 2 years of storage. Among the seed treatments, on an average, after 2 years of seed storage, significantly (P<0.05) higher values were recorded by all the seed treatments over the control. However, seed treated with Mancozeb @ 2g/kg of seed recorded the significantly highest germination percentage (71.50 %) and it was at par with Neem leaf powder @ 10 g / kg seed (70.67%) and Carbendazim @ 2g /kg seed (69.67%) after 2 years of storage. The germination percentage noted in control treatment was 33.17 per cent after 2 years of storage. An ISTA standard for germination in soybean is 70 per cent. Most of the interactions effects were found significant (P<0.05) for all the traits studied.


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