Effect of different storage conditions and seed treatments on seed viability in soybean [Glycine max (L.) Merr.]

The present investigation was carried out in laboratory of the Department of Seed Science and Technolo-gy, College of Agriculture, Junagadh Agricultural University, Junagadh from the April 2013 to April 2015, wherein two kg of freshly harvested quality seed of soybean cv. Gujarat Junagadh Soybean 3 having high germination percentage and low moisture content (below 8%) was taken for each repetition and for each combination of treat-ments. The treatment consisted of two storage conditions (C) viz., C1 (Ambient temperature) and C2 (Cold storage at 7oC + 2oC), and five seed treatments (S) viz., S1 = Control, S2 = Carbendazim @ 2g/kg seed, S3 = Mancozeb @ 2g/ kg seed, S4 = Neem leaf powder @ 10g/kg seed, and S5 = Neem Oil @ 5 ml/kg seed. The experiment was carried out using Completely Randomized Design (Factorial) repeated three times. After proper mixing or smearing the seeds as per the treatments, seeds were packed in cloth bag and kept in laboratory under two different storage conditions. Observations were recorded at 90 days interval on germination (%), root length (cm), shoot length (cm), seedling dry weight (g), seed vigour index I, seed vigour index II and seed moisture content (%). The results revealed that storage condition (C) and seed treatments (S) exhibited significant differences almost for the all the traits for germination and seedling parameters after 2 years of storage. The results of soybean seed stored in two different storage conditions showed that on an average, the seed stored under cold storage (70C + 20C) noted higher values for all the traits studied except seed moisture content after 2 years of storage. Among the seed treatments, on an average, after 2 years of seed storage, significantly (P<0.05) higher values were recorded by all the seed treatments over the control. However, seed treated with Mancozeb @ 2g/kg of seed recorded the significantly highest germination percentage (71.50 %) and it was at par with Neem leaf powder @ 10 g / kg seed (70.67%) and Carbendazim @ 2g /kg seed (69.67%) after 2 years of storage. The germination percentage noted in control treatment was 33.17 per cent after 2 years of storage. An ISTA standard for germination in soybean is 70 per cent. Most of the interactions effects were found significant (P<0.05) for all the traits studied.

Download Full-text

Performance of neem products on the storability of mungbean[Vigna radiata (L.) Wilczek] seeds

Indian Journal of Agricultural Research ◽

10.18805/ijare.v50i6.6675 ◽

2016 ◽

Vol 50 (6) ◽

Author(s):

Babariya C. A. ◽

Patel J. B. ◽

Ribadiya Ribadiya ◽

K. H. Sondarva Jyoti ◽

Bhatiya V. J.

Keyword(s):

Moisture Content ◽

Germination Percentage ◽

Shoot Length ◽

Seed Kernel ◽

Neem Seed ◽

Seed Moisture Content ◽

Seed Vigour ◽

Neem Cake ◽

Significant Difference ◽

Vigour Index

The present investigation was carried out in laboratory of the Department of Seed Science and Technology, College of Agriculture, Junagadh Agricultural University, Junagadh from August, 2013 to June, 2015, wherein two kg of freshly harvested quality seed of mungbean cv. GM 4 having high germination percentage and low moisture content (below 8%) was taken for each repetition and given the treatments. The experiment was carried out using completely randomized design (CRD). After proper mixing or smearing, seeds were packed in 500 gauge polythene bags and kept in laboratory under ambient condition. Observations were recorded at bimonthly interval on germination (%), root length (cm), shoot length (cm), seedling dry weight (g), seed vigour index I, seed vigour index II and seed moisture content (%). The results indicated that all the treatments exhibited significant difference for all the traits studied except shoot length after two years of storage. The Indian Minimum Seed Certification Standards in mungbean for germination percentage is 75 per cent. Looking to the germination percentage and Seed vigourindex I and II, among all the treatments, T1 (Neem Seed kernel powder @ 5 g/kg seed), T2 (Neem seed kernel powder @ 10 g/kg seed), T3 (Neem Cake @ 5g/kg Seed) and T4 (Neem Cake @ 10g/kg Seed) recorded the higher values for these traits and can be recommended as a seed treatment before storage for maintaining the seed germination and seedling vigour for more than two years.

Download Full-text

Effect of storage conditions, packing materials and seed treatments on viability and seedling vigour of onion (Allium cepa l.) seeds

Journal of Applied and Natural Science ◽

10.31018/jans.v9i2.1321 ◽

2017 ◽

Vol 9 (2) ◽

pp. 1054-1067

Author(s):

J. B. Patel ◽

C. A. Babariya ◽

Jyoti Sondarva ◽

K. H. Ribadiya ◽

V. J. Bhatiya

Keyword(s):

Cold Storage ◽

Storage Conditions ◽

Germination Percentage ◽

Seed Treatments ◽

Seedling Vigour ◽

Packing Materials ◽

High Germination ◽

Allium Cepa L ◽

Leaf Powder ◽

Polyethylene Bags

The present investigation was carried out from July 2013 to July 2015, wherein 100 g of fresh quality seed of onion cv. GWO 1 was having high germination percentage and moisture content below 8 per cent. The treatment consisted of two storage conditions (C) viz., C1 (Ambient temperature) and C2 (Cold storage at 70C + 20C); two packing materials (P) viz., P1 = Cloth Bag and P2 = Polythene Bag (500 gauge = 125 µ), and five seed treatments (S) viz., S1 = Control, S2 = Carbendazim @ 2g/kg seed, S3 = Mancozeb @ 2g/kg seed, S4 = Thirum @ 3g/kg seed, and S5 = Neem leaf powder @ 10g / kg seed. After proper mixing or smearing the seeds as per the treatments, seeds were packed and stored as per treatments. Observations were recorded at 90 days interval on viability and vigour parameters. The results revealed that seed stored under cold storage (7±2 °C) and in polyethylene bags (500 gauge) noted significantly higher values for all the characters even after two years of storage. All the treatment combinations of seed stored under cold storage gave more than 70 per cent germination (As per ISTA standard) even after two years of storage, of which, seed treated with thirum @ 3g/kg seed was the best treatment. Therefore, it can be concluded that seed of onion can be stored up to two year in cold storage packed in polyethylene bag without or with seed treatment without deterioration in germination and seedling vigour.

Download Full-text

Plantability and moisture content of naked and pelleted seeds of supersweet (Sh2) corn during cold storage conditions

Revista Brasileira de Sementes ◽

10.1590/s0101-31222012000100005 ◽

2012 ◽

Vol 34 (1) ◽

pp. 39-46 ◽

Cited By ~ 4

Author(s):

André de Oliveira Lagôa ◽

Adriano César Ferreira ◽

Roberval Daiton Vieira

Keyword(s):

Moisture Content ◽

Cold Storage ◽

Research Work ◽

Apparent Volume ◽

Storage Conditions ◽

Irregular Shape ◽

Seed Moisture Content ◽

Seed Moisture ◽

Physical Attributes ◽

Supersweet Corn

The supersweet (Sh2) corn seeds have a thin tegument and an irregular shape, which hinder the sowing procedure. As a function of those factors, the significance of using the pelleting process to improve sowing and standardize the stand of plants in the field without the need of thinning within the row is emphasized. Although this technique has already been known for many years, little has been studied on the effect of such procedure on the plantability and on the moisture content of the supersweet corn seeds. Therefore, this research work aimed at evaluating the effects of pelleting on the moisture content of the seeds along the storing process, on the occurrence of gaps on seed deposition and on the dropping of double seeds, with the aid of a testing seeder. Other physical attributes of seeds and pellets were also evaluated through tests of fragmentation, screen retention, mass of a thousand seeds and apparent volume. Results have shown an increase on the mass and on the volume of the pelleted seeds that presented highly resistance to cracks and breaking. The pelleting was efficient on the reduction of gaps and on the deposition of double seeds at sowing, thus providing highly satisfactory results for these attributes. An increase on the seed moisture content was also observed as a function of storage with a significant reduction on this factor after the pelleting procedure. Results allow concluding that the pelleting process reduces seed moisture content and improves plantability, positively affecting the efficiency of the sowing process.

Download Full-text

Germination of Nonstratified Japanese Tree Lilac Seeds as Influenced by Seed Capsule Maturity and Moisture Content

HortTechnology ◽

10.21273/horttech.24.2.177 ◽

2014 ◽

Vol 24 (2) ◽

pp. 177-180 ◽

Cited By ~ 1

Author(s):

Todd P. West ◽

Samuel L. DeMarais ◽

Chiwon W. Lee

Keyword(s):

Moisture Content ◽

Germination Percentage ◽

Seedling Production ◽

Fresh Weight ◽

Seed Moisture Content ◽

Seed Collection ◽

The North ◽

Seed Moisture ◽

Embryo Dormancy ◽

Seed Stratification

Temperate-zone woody plant species generally require seed stratification to overcome embryo dormancy. Embryo dormancy is variable in japanese tree lilac (Syringa reticulata) with a recommendation of 30- to 90-days stratification at 1–5 °C. Cultivar propagation generally is done by grafting onto seedling rootstocks. It would be advantageous to rootstock seedling production to be able to reduce or eliminate the need for seed stratification to increase germination numbers as well as having production moved from field beds to greenhouses for quicker production of high-quality rootstock plants suitable for grafting. Research objective of this study was to determine if “green” seed could be used without the need of stratification for japanese tree lilac seedling production. Seed capsule fresh weight and seed moisture content were evaluated to determine if these factors could be used as predictors of germination percentages. Seed was randomly collected at the North Dakota State University campus in Fargo, ND, for seven consecutive weeks starting in Sept. 2011 and 2012. Germination and seed moisture tests were performed weekly. Germination percentage was highest (89.5%) at week 2 and steadily decreased to 0% at week 7. Germination percentages were 77.5%, 89.5%, 78.5%, 67%, 24.5%, 1.5%, and 0% for consecutive collection weeks 1–7, respectively. Seed moisture content was 59.0%, 52.6%, 49.8%, 51.8%, 44.5%, 27.4%, and 8.6% for collection weeks 1–7, respectively. Germination percentage was directly correlated with seed moisture content and decreased as seed capsules matured (natural drying and splitting of capsule seem to disperse seed) during the fall season. Data suggest that timing of fall seed collection from japanese tree lilac is critical and must be done before maturation of the seed capsule to avoid the stratification requirement. Seed capsules with an average fresh weight higher than 0.2 g and seed moisture content greater than 50% produced the highest germination rates without requiring stratification.

Download Full-text

Seed health of common bean stored at constant moisture and temperature

Scientia Agricola ◽

10.1590/s0103-90162008000600007 ◽

2008 ◽

Vol 65 (6) ◽

pp. 613-619 ◽

Cited By ~ 5

Author(s):

Fabiana Gonçalves Francisco ◽

Roberto Usberti

Keyword(s):

Moisture Content ◽

Common Bean ◽

Storage Conditions ◽

Quality Parameters ◽

Main Concern ◽

Fusarium Spp ◽

Seed Moisture Content ◽

Seed Moisture ◽

Seed Health ◽

Penicillium Spp

Fungal incidence in stored common bean (Phaseolus vulgaris L.) is the main concern in order to preserve seed health and viability. The main aim of this study was to analyse these quality parameters in hermetically stored seeds at 10.2, 13.1, 16.2, 18.5% moisture content (MC) and 25, 30, 35, 40°C, through seed germination and health tests. Water activity recorded at 10.2 and 18.5% MC were 0.448 and 0.700, respectively. Low seed moisture content reduced Alternaria spp. incidence at 25-30°C. Highest incidence of Fusarium spp. (7.5%) occurred at 16.2% MC and 35-40°C. Highest incidences of Rhizoctonia spp. (8-10%) were recorded at 16.2-18.5% MC and 30-40°C. Penicillium spp. and Aspergillus spp. were predominant throughout the experiment and the highest incidences (80-100%; 20-30%, respectively) were scored at 18.5% MC and 30-35°C and 13.1-18.5% MC at 35°C, respectively. The higher the seed MC the higher the fungi incidence while lower seed MC decreased the incidences by 25%. Storage conditions below 30°C and 13.0% MC appear suitable to preserve common bean seed in relation to viability and health, up to a 8-month period.

Download Full-text

Effect of different storage materials on the seed temperature, seed moisture content and germination of wheat under farmer’s field condition of Kailali district, Nepal

Agricultural Science and Technology ◽

10.15547/ast.2019.04.060 ◽

2019 ◽

Vol 11 (4) ◽

pp. 352-355

Author(s):

N. Khatri ◽

D. Pokhrel ◽

B.P. Pandey ◽

K.R. Pant ◽

M. Bista

Keyword(s):

Moisture Content ◽

Block Design ◽

Germination Percentage ◽

Quality Parameters ◽

Seed Moisture Content ◽

Quality Performance ◽

Storage Materials ◽

Seed Moisture ◽

Randomized Complete Block Design ◽

Long Time

Abstract. A study was conducted at four Village Development Committees (Masuriya, Udashipur, Basauti and Pahalmanpur) of Kailali dis- trict, Far western Region, Nepal in 2016/17 to find out the effect of different storage materials on the seed moisture content and germination percentage of wheat. Seeds of wheat were packed using five storage materials (metal seed bin, earthen pot, purdue improved crop storage / Pics/ bag, gunny bag and super grain bag). The stored seed samples were drawn at three-month intervals starting from May 2016 to January 2017. The experiment was arranged in Randomized Complete Block Design (RCBD) in four replications. The results of analysis of variance revealed that the effect of storage materials was significant (P≤0.05) on seed moisture content and seed germination of wheat. After nine months of storage, the seeds stored in Pics bag recorded the lowest moisture (13.3%) and temperature (26.4°C), and the highest germination percentage (91.75%). The lowest quality performance (high moisture and temperature, low germination) of stored seeds was observed in an earthen pot. From the results obtained, it was concluded that wheat seed could be stored for a long time in Pics bag without deteriorating its quality parameters.

Download Full-text

Moisture level and storage container effects on seed quality of soybean genotypes under ambient condition

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v44i4.45698 ◽

2020 ◽

Vol 44 (4) ◽

pp. 631-640

Author(s):

MR Ali ◽

MM Rahman ◽

M Asaduzzaman ◽

MAH Khan ◽

J Rahman

Keyword(s):

Moisture Content ◽

Seed Quality ◽

Agricultural Research ◽

Seed Moisture Content ◽

Seed Moisture ◽

Vigour Index ◽

Soybean Genotypes ◽

Initial Seed ◽

And Storage

The experiment was conducted at the Seed Laboratory, Regional Agricultural Research Station, Bangladesh Agricultural Research Institute, Jamalpur in 2013 to study the effect of seed moisture content and storage containers on seed quality of soybean genotypes. Three genotypes of soybean (AGS 191, ASET 93 and Shohag), four initial seed moisture content (6, 8, 10 and 12%) and two types of storage containers (polythene bag and glass jar) were included in the experimental treatment. Seeds of soybean genotypes was stored at ambient condition were temperature ranged from 15.97 to 29.37 0C, relative humidity ranged from 75.21 to 86.23% and rainfall ranged from 0.00 to 425mm during the whole storage period. Seed moisture content (%), germination (%) and vigour index were recorded at 50, 100, 150 and 200 days after storage (DAS). Result showed that final seed moisture content increased with the increase of initial seed moisture content. Genotype AGS 191 showed the highest germination (%) and vigour index. Seeds stored in polythene bag or glass jar showed similar performance for germination (%) and vigour index. Highest seed moisture content significantly reduced the germination and vigour index errespective of containers. The results indicate that soybean seed can be stored safely for at least 200 days maintaining >80% germination and high vigour when stored in polythene bag or glass jar with 6-8% initial moisture content at ambient room temperature and relative humidity. Bangladesh J. Agril. Res. 44(4): 631-640, December 2019

Download Full-text

CHROMOSOME DAMAGE INDUCED BY ARTIFICIAL SEED AGING IN BARLEY. I. GERMINABILITY AND FREQUENCY OF ABERRANT ANAPHASES AT FIRST MITOSIS

Canadian Journal of Genetics and Cytology ◽

10.1139/g81-029 ◽

1981 ◽

Vol 23 (2) ◽

pp. 267-280 ◽

Cited By ~ 23

Author(s):

Minoru Murata ◽

Eric E. Roos ◽

Takumi Tsuchiya

Keyword(s):

Moisture Content ◽

Seed Storage ◽

Germination Percentage ◽

Mitotic Division ◽

Root Tips ◽

Hordeum Vulgare L ◽

Seed Moisture Content ◽

Seed Aging ◽

Seed Moisture ◽

Higher Temperature

In order to study the genetic changes which occur during seed storage, barley (Hordeum vulgare L. 'Himalaya') seeds were subjected to artificial aging using six combinations of temperature (21 °C, 32 °C, and 38 °C) and seed moisture content (12% and 18%). With increasing time in storage, germination of the seeds was delayed and reduced. Abnormal seedlings without roots also occurred with increased storage. Higher temperature and seed moisture content induced rapid loss of germinability. At the first mitotic division in the root tips, the frequency of aberrant anaphases and of roots with aberrations increased with increased storage time. The frequencies of aberrant anaphases and of roots with aberrations were also increased by higher temperature and seed moisture content. Frequencies of aberrant anaphases and of roots with aberrations were negatively correlated with germination percentage. This indicated that the frequency of chromosomal aberrations induced by seed aging might be estimated from the germination percentages.

Download Full-text

Halogens, alcohols and potassium permanganate extend the storability of hot pepper seeds (Capsicum annuum l.) under accelerated ageing conditions

Journal of Horticultural Research ◽

10.2478/johr-2013-0032 ◽

2013 ◽

Vol 21 (2) ◽

pp. 131-138

Author(s):

Deepu Mathew ◽

S.D. Doijode ◽

K. Madhavi Reddy

Keyword(s):

Moisture Content ◽

Potassium Permanganate ◽

Seed Quality ◽

Seed Viability ◽

Dry Weight ◽

Accelerated Ageing ◽

Hot Pepper ◽

Seed Moisture Content ◽

Seed Vigour ◽

Seed Moisture

ABSTRACT The feasibility of extending the storability of fresh hot pepper seeds, by modifying the storage atmosphere, using the respiration inhibitors and seed desiccants was examined. Halogens such as chlorine, bromine and iodine and methanol, ethanol and potassium permanganate were used for a period of 22 months under accelerated ageing conditions of high seed moisture and temperature. The various seed quality parameters - viability, vigour, speed of germination, seedling dry weight, and moisture content were evaluated. In the untreated control, decreased seed viability was observed within 10 months of storage. However, iodine and chlorine were proved effective in retaining high seed viability up to 90.7 and 88.0%, respectively, even after 22 months of storage. Chlorine treatment was able to retain the seed vigour (904.0), iodine (766.4), KMnO4 (754.4) and methanol (566.7) whereas the value of vigour index in control was 72.0. Chlorine and iodine were on par in their ability to maintain the speed of seed germination (25.2 and 24.8, respectively), followed by KMnO4 (20.2). Seedling dry weight was proved as a parameter not sensitive in estimating the seed quality since even after 22 months storage of seeds at atmospheres of chlorine, iodine, KMnO4 and methanol did not show any differences. KMnO4 sharply reduced the seed moisture content from 9.83 to 7.89% providing better storability over the control.

Download Full-text

Drying and physiological quality of palheteira seeds (Clitoria fairchildiana R.A. Howard)

Research Society and Development ◽

10.33448/rsd-v9i9.8157 ◽

2020 ◽

Vol 9 (9) ◽

pp. e858998157

Author(s):

Érica Coutinho David ◽

Bressa Karolina Dias Cardoso ◽

Josiene Amanda dos Santos Viana ◽

Eniel David Cruz

Keyword(s):

Moisture Content ◽

Seed Quality ◽

Seed Storage ◽

Germination Percentage ◽

Hot Water ◽

Degraded Land ◽

Seed Moisture Content ◽

Seed Moisture ◽

Physiological Quality

Knowledge about seed storage behavior is important to preserve plant species. Clitoria fairchildiana R.A.Howard is an endemic species with medicinal properties, it is used in the recovery of degraded land. The objective of this study is to evaluate the effect of drying on the physiological quality of C. fairchildiana seeds. Seeds were oven dried along with silica gel for 18 days and in intervals of 2 or 3 days, samples were removed to assess the seed moisture content. This was done in an oven at 105±3 oC for 24h, using four replicates with 10 seeds each. After undergoing the above-mentioned treatment, a sample of seeds was stored in the freezer (-18 ºC) for 3 months. Sowing was carried out in plastic trays in a sand and sawdust mixture (1:1), previously sterilized in hot water (100 oC) for two hours. Seeds were left to germinate in a laboratory with no temperature and relative humidity control. Germination tests were performed over 14 days with daily counts of the number of emerged seedlings. The percentage of seed germination, abnormal seedlings and dead seeds were obtained 14 days after sowing. The experimental design was completely randomized with four replications of 25 seeds. The reduction of seed moisture content from 18.5% to 5.6% affected physiological seed quality causing a reduction in the germination percentage and an increase in dead seeds and abnormal seedling percentage. C. fairchildiana seeds are classified as intermediate and they can be desiccated up to 8.6% with no reduction in physiology quality.

Download Full-text