scholarly journals Evaluation of urinary L-FABP as an early marker for diabetic nephropathy in type 2 diabetic patients

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Duong Thi Thuy Ngan ◽  
Nguyen Gia Binh ◽  
Le Thi Huong Lan ◽  
Cuc Thi Thu Nguyen ◽  
Phung Thanh Huong
Keyword(s):  
Renal Function ◽  
Diabetic Nephropathy ◽  
Early Detection ◽  
Urinary Albumin ◽  
Control Group ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Fatty Acid Binding ◽  
Type 2 Diabetic

Summary Background Albuminuria is the standard biomarker for the diagnosis of diabetic nephropathy (DN). However, some patients with persistent microalbuminuria still progress to chronic kidney disease, raising the question of finding a better biomarker. This study aimed to evaluate the correlation of urinary liver-type fatty acid-binding protein (L-FABP) levels with renal function and to compare the role of urinary albumin-to-creatinine ratio (ACR) with urinary L-FABP in early detection of DN in type 2 diabetic patients. Methods The cross-sectional study was done on 106 type 2 diabetic patients and 30 non-diabetic people. L-FABP was measured with the Latex enhanced immunoturbidimetric technique. Results There was a strong and negative correlation between the urine L-FABP levels and eGFR (r = -0.606, p<0.001). The urinary L-FABP levels were significantly higher (p<0.001) in the normoalbuminuria diabetic group than the non-diabetic control group. The ROC-curve analyses in the diabetic patients and the normoalbuminuria diabetic patients showed that the AUCL-FABP was remarkably higher (p<0.001) than the AUCACR. An optimal cutoff value of 5 mg L-FABP/g Cr (with the sensitivity of 98.1% and specificity of 90%) and of 4.3 mg L-FABP/g Cr (with the sensitivity of 100% and specificity of 86.67%) was set to detect DN in the diabetic patients and the normoalbuminuria diabetic patients, respectively. Conclusions The change in urinary L-FABP levels happened earlier than in urinary albumin during renal function impairment. Urinary L-FABP can be used as a better indicator than ACR for early detection of DN in type 2 diabetes.

scholarly journals Serum Klotho protein level in type 2 diabetic patients depending on the renal function

2020 ◽  
pp. 60-66
Author(s):  
I. Topchii ◽  
P. Semenovykh ◽  
T. Shcherban ◽  
V. Galchinska ◽  
K. Savicheva
Keyword(s):  
Acute Coronary Syndrome ◽  
Renal Function ◽  
Diabetic Nephropathy ◽  
Control Group ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Coronary Syndrome ◽  
Klotho Protein ◽  
Type 2 Diabetic

The study aimed to assess serum Klotho protein level in type 2 diabetic patients depending on kidney function. Methods. This observational study included 72 patients with diabetes mellitus (DM) and 26 patients with acute coronary syndrome. The control group consisted of 20 healthy subjects. Depending on the presence of albuminuria and glomerular filtration rate (GFR), the diabetics were divided into the following groups: group I included the patients with normal GFR and without albuminuria (n = 25); group ІІ consisted the patients with normal GFR and albuminuria (n = 23); group III – the patients with reduced GFR and albuminuria (n = 24) and group ІV included the patients with acute coronary syndrome (n = 26). The GFR was calculated using the CKD EPI formula (KDIGO 2012). The concentration of Klotho protein was determined by enzyme-linked immunosorbent assay. Results. The development of diabetic nephropathy in type 2 diabetic patients accompanied by a significant decrease of soluble Klotho compared with the controls and the patients of the1-st group. The level of Klotho protein in the group of patients with albuminuria decreased to (490.66 ± 58.76) pg/ml (p <0.05). The lowest concentration of Klotho (443.58 ± 46.92) pg/ml was found in the advanced stages of diabetic nephropathy, namely in patients with albuminuria and impaired renal function. Moreover, a significantly decreased serum Klotho was observed in acute coronary syndrome group in comparison with the control group (p <0.05). There were inverse correlations of Klotho concentration with urinary albumin and blood creatinine levels and a direct correlation between Klotho and GFR. Conclusions. The obtained data indicated the key role of Klotho protein in the formation of renal pathology in type 2 diabetes and the feasibility of practical use of Klotho determination as an early diagnostic marker of renal disorders and cardiovascular risk assessment. The strategies improving Klotho production may be useful in the reduction of both renal and vascular lesions progression in type 2 diabetic patients.

Urinary Albumin as an Indicator of Diabetic Nephropathy Lesions in Japanese Type 2 Diabetic Patients

Nephron ◽  
2002 ◽  
Vol 91 (2) ◽  
pp. 292-299 ◽  
Author(s):  
Tatsumi Moriya ◽  
Rika Moriya ◽  
Yoshitada Yajima ◽  
Michael W. Steffes ◽  
Michael Mauer
Keyword(s):  
Diabetic Nephropathy ◽  
Urinary Albumin ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetic

scholarly journals Serum Adipocyte Fatty Acid-Binding Protein 4 Levels Are Independently Associated with Radioisotope Glomerular Filtration Rate in Type 2 Diabetic Patients with Early Diabetic Nephropathy

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaoqing Ni ◽  
Yunjuan Gu ◽  
Haoyong Yu ◽  
Shenqi Wang ◽  
Ying Chen ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Diabetic Patients ◽  
Creatinine Ratio ◽  
Type 2 Diabetic Patients ◽  
Fatty Acid Binding ◽  
Early Diabetic Nephropathy ◽  
Type 2 Diabetic

Serum fatty acid-binding protein 4 (FABP4) has been linked to renal dysfunction. This study evaluated the association between serum FABP4 and the radioisotope glomerular filtration rate (rGFR) in type 2 diabetic patients (T2DM) with early diabetic nephropathy. Twenty healthy controls and 172 patients with T2DM were enrolled. Serum FABP4 and renal impairment biomarkers including urinary albumin-to-creatinine ratio (UACR), serum retinal-binding protein 4 (RBP4), urinary cystatin C-to-creatinine ratio (CysC/Cr), and neutrophil gelatinase-associated lipocalin-to-creatinine ratio (NGAL/Cr) were measured. Diethylenetriaminepentaacetic acid (99mTc-DTPA) was used to test rGFR. Serum FABP4 levels were higher in T2DM patients compared with the controls. There was no significant correlation between serum FABP4 and UACR in patients with T2DM. Multivariate stepwise regression analysis showed that, in patients with T2DM, FABP4 was significantly associated with rGFR while CysC/Cr and RBP4 were significantly associated with UACR independently. But UACR had no independent association with rGFR. NGAL/Cr had no significant correlation with either rGFR or UACR. FABP4 might be an early biomarker for diabetic nephropathy if combined with UACR.

scholarly journals Effects of Apolipoprotein E Isoforms in Diabetic Nephropathy of Chinese Type 2 Diabetic Patients

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
YongWei Jiang ◽  
Liang Ma ◽  
ChengWu Han ◽  
Qian Liu ◽  
Xiao Cong ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Genetic Polymorphism ◽  
Apolipoprotein E ◽  
Group Versus ◽  
Allelic Frequency ◽  
Control Group ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetic

Diabetic nephropathy (DN) is one of the major chronic complications of diabetes. Genetic polymorphism of Apolipoprotein E (ApoE) has been proposed to participating in DN. The purpose of the study was to evaluate the relationship between ApoE genetic polymorphism and the presence of DN in Chinese type 2 diabetic patients. We studied 845 diabetic patients who were divided into DN group (n=429) and control group (n=416). ApoE genotype was determined by ApoE genotyping chip and the plasmatic biochemical characterization was performed on all subjects. There were differences (P<0.001) in HbA1c, creatinine, and urinary albumin between the two groups. The ApoEε2 allelic frequency was 7.69% in DN group versus 3.49% in control group (OR = 2.22, 95% CI = 1.41–3.47, andP<0.05), as expected, ApoE E2/E2 and E2/E3 genotype frequency were higher in DN group (13.75% versus 6.49%,P<0.05). The ApoEε4 allelic frequency was 7.93% in DN group versus 11.54% in control group (OR = 0.70, 95% CI = 0.50–0.97, andP<0.05), and DN group presented a lower frequency of ApoE E3/E4 and E4/E4 genotype frequency (14.91% versus 19.96%,P<0.05). These results suggest ApoEε2 allele may be a risk factor; however ApoEε4 allele may play a protective role of DN in Chinese type 2 diabetic patients.

scholarly journals VISFATIN LEVEL DISTURBANCES IN PERIPHERAL BLOOD OF PATIENTS WITH DIABETIC NEPHROPATHY

2014 ◽  
pp. 25-30
Author(s):  
I.I. Topchii ◽  
P.S. Semenovykh ◽  
V.YU. Galchinskaya ◽  
N.V. Yefimova
Keyword(s):  
Diabetic Nephropathy ◽  
Blood Plasma ◽  
Peripheral Blood ◽  
Control Group ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Visfatin Level ◽  
Type 2 Diabetic ◽  
In Blood Plasma

 Introduction. Recent studies suggest that visfatin participates in pathogenesis of vascular diabetic complications, in particular diabetic nephropathy (DN). The aim of the present research - definition of visfatin level in peripheral blood of type 2 diabetic patients taking to account renal function disturbances and body mass index (BMI). Materials and methods. 94 type 2 diabetic patients with different stages of DN and 10 healthy subjects (control group) were observed. Visfatin concentration in blood plasma was determined using immunoassay kit. Results. An essential increase of visfatin level in blood plasma took place already in initial stages of the DN. Progressing of the disease was accompanied by more expressed growth of visfatin concentration. In patients with high BMI substantial increase of visfatin level when compared with those with normal IMT was determined. We established strong correlations between visfatin concentrations, urinary albumin levels and blood creatinine concentrations and negative correlations with glomerular filtration rate. Conclusions: Our findings testify that visfatin level displays a functional kidney state and may be used as addition to traditional methods of patients examination.

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