scholarly journals Mutations of p53 gene in canine sweat gland carcinomas probably associated with UV radiation

2021 ◽  
Vol 65 (4) ◽  
pp. 519-526
Author(s):  
Agnieszka Jasik ◽  
Anna Kycko ◽  
Monika Olech ◽  
Krzysztof Wyrostek ◽  
Anna Śmiech ◽  
...  
Keyword(s):  
Uv Radiation ◽  
Sweat Gland ◽  
Pcr Amplification ◽  
P53 Gene ◽  
Skin Tumours ◽  
Epidemiological Aspect ◽  
Malignant Skin ◽  
Apocrine Sweat Gland ◽  
Exon 4 ◽  
Laser Capture

Abstract Introduction Apocrine sweat gland carcinomas (ASGCs) are rare malignant skin tumours in dogs and humans. The literature published so far focuses mostly on the clinico-epidemiological aspect of these tumours, but little is known about their pathogenesis. In this study we aimed to determine whether the p53 gene is involved in the carcinogenesis of the apocrine sweat gland in dogs and whether ultraviolet radiation (UV) is related to it. Material and Methods Forty canine ASGCs were submitted to laser capture microdissection to isolate neoplastic cells, from which DNA was subsequently extracted. PCR amplification and sequencing of p53 exons 2–8 was then performed, followed by computer analysis of the obtained sequences. Results Sixteen mutations within the p53 gene were found in 13 tumours. The mutations involved C → T, T → C, G → A, and CC → TT transitions, C → G transversion and adenine deletion, which are gene alteration types known to be related to UV radiation in the process of skin carcinogenesis in humans. Six of the thirteen tumour cases displayed the C → T transitions in the same location in exon 4 and three of the thirteen cases displayed T → C in the same location in exon 5. Conclusion The results of the present study indicate both the participation of the p53 gene and the influence of UV radiation in the formation of ASGCs in dogs.

scholarly journals Identifying new variation at the J locus, previously identified as e6, in long juvenile ‘Paranagoiana’ soybean

2021 ◽  
Author(s):  
Nour Nissan ◽  
Elroy R. Cober ◽  
Michael Sadowski ◽  
Martin Charette ◽  
Ashkan Golshani ◽  
...  
Keyword(s):  
Single Gene ◽  
Pcr Amplification ◽  
Gene Control ◽  
Grain Yields ◽  
Short Day ◽  
Exon 4 ◽  
Short Days ◽  
Kasp Markers

Abstract Key message A previously identified soybean maturity locus, E6, is discovered to be J, with the long juvenile allele in Paranagoiana now deemed j−x. Abstract Soybean grown at latitudes of ~20° or lower can produce lower grain yields due to the short days. This limitation can be overcome by using the long juvenile trait (LJ) which delays flowering under short day conditions. Two LJ loci have been mapped to the same location on Gm04, J and E6. The objective of this research was to investigate the e6 allele in ‘Paranagoiana’ and determine if E6 and J are the same locus or linked loci. KASP markers showed that e6 lines did not have the j−1 allele of LJ PI 159925. A population fixed for E1 but segregating for E6, with e6 introgressed from Paranagoiana, showed single gene control for flowering and maturity under short days. Sequencing Glyma.04G050200, the J gene, with long amplification Taq found that the e6 line ‘Paranagoiana’ contains a Ty1-copia retrotransposon of ~10,000 bp, inserted within exon 4. PCR amplification of the cDNA of Glyma.04G050200 also showed differences between the mRNA sequences (presence of insertion in j−x). Hence, we conclude that the loci E6 and J are one locus and deem this new variation found in Paranagoiana as j−x.

Ultrasound-guided peripheral nerve blocks are useful for resections of malignant skin tumours on the leg

2017 ◽  
Vol 27 (1) ◽  
pp. 70-72
Author(s):  
Kenji Yokota ◽  
Takaaki Matsumoto ◽  
Yoshie Murakami ◽  
Kaori Ando ◽  
Masashi Akiyama
Keyword(s):  
Peripheral Nerve ◽  
Ultrasound Guided ◽  
Nerve Blocks ◽  
Skin Tumours ◽  
Peripheral Nerve Blocks ◽  
Malignant Skin

Skin diseases affecting the oro-facial region

2018 ◽  
Author(s):  
Max Robinson ◽  
Keith Hunter ◽  
Michael Pemberton ◽  
Philip Sloan
Keyword(s):  
Skin Diseases ◽  
Systemic Disease ◽  
Lymphatic Vessels ◽  
Medical Practitioner ◽  
Skin Tumours ◽  
Facial Region ◽  
Malignant Skin ◽  
Common Skin ◽  
The Face ◽  
Direct Inoculation

Examination of the face and hands can identify significant skin diseases and also provide clues to the presence of underlying systemic disease. Many patients ignore even malignant skin tumours because they are often painless, subtle in appearance, and may be slow-growing. Dental healthcare professionals should be aware of how to recognize malig­nant skin tumours. If suspicious, but unsure of the nature of the lesion, the patient should be referred to their general medical practitioner for further evaluation. If malignancy is obvious, then an urgent referral to an appropriate specialist (dermatologist, plastic surgeon, or oral and max­illofacial surgeon) should be made using the ‘2-week wait’ (2WW) path­way (Chapter 1). Benign lesions and inflammatory diseases are more common and are important considerations in the differential diagnosis of head and neck skin abnormalities. It is important that the dental healthcare professional should be able to recognize common skin infections involving the oro-facial region. Some infections, such as erysipelas, can mimic cellulitis associated with a dental infection. When infection is diagnosed, it is vital to consider the underlying or predisposing factors, as these may be not only important diagnoses, but also may require treatment to achieve an effective clin­ical outcome. The adage ‘infection is the disease of the diseased’ is a useful reminder when dealing with patients presenting with infection. Direct inoculation of Streptococcus into skin through minor trauma is the most common initiating factor for erysipelas, which occurs in iso­lated cases. Infection involves the upper dermis and, characteristically, spreads to involve the dermal lymphatic vessels. Clinically, the disease starts as a red patch that extends to become a fiery red, tense, and indurated plaque. Erysipelas can be distinguished from cellulitis by its advancing, sharply defined borders and skin streaking due to lymphatic involvement. The infection is most common in children and the elderly, and whilst classically a disease affecting the face, in recent years it has more frequently involved the leg skin of elderly patients. Although a clinical diagnosis can be made without laboratory testing, and treat­ment is antibiotic therapy, when the diagnosis is suspected in dental practice, referral to a medical practitioner is recommended.

Dermatology

2020 ◽  
pp. 559-616
Author(s):  
Chantal Simon ◽  
Hazel Everitt ◽  
Françoise van Dorp ◽  
Nazia Hussain ◽  
Emma Nash ◽  
...  
Keyword(s):  
General Practice ◽  
Skin Cancer ◽  
Fungal Infection ◽  
Sweat Gland ◽  
Skin Infection ◽  
Skin Tumours ◽  
Nail Changes ◽  
Skin Erythema ◽  
Keratinization Disorders ◽  
Skin Conditions

This chapter in the Oxford Handbook of General Practice explores dermatology in general practice. It covers skin assessment, treatment of skin conditions, changes in skin colour and eruptions, itching and blistering of the skin, erythema, pigmentation disorder, hair and sweat gland problems, nail changes, and atopic and other eczemas. It discusses ulcers, urticaria, angio-oedema, acne, psoriasis, lichen planus, keratinization disorders, pityriasis, and seborrhoeic warts. It examines sunlight and the skin, benign skin tumours, and skin cancer. It also explores bacterial skin infection, viral skin infection, fungal infection, and infestation.

Mutational Analysis Revealed Polymorphic Variants in Exon 3 and Exon 4 Of P53 Gene in Cervical Cancer

2020 ◽  
Vol 106 (1_suppl) ◽  
pp. 18-18
Author(s):  
Omotoso Olabode ◽  
A. Gbadegesin Michael ◽  
A. O. Oluwasola Timothy ◽  
A. Okolo Clement ◽  
O. Ogun Gabriel ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Screening ◽  
Sequence Alignment ◽  
Multiple Sequence Alignment ◽  
Genomic Dna ◽  
P53 Gene ◽  
Reference Sequence ◽  
Multiple Sequence ◽  
Polymorphic Variants ◽  
Exon 4

This research study explored the mutations in the exon 3 and exon 4 of the P53 gene in cervical cancer. Genomic DNA was isolated and purified from cervical tissue biopsies obtained from patients reporting at the University College Hospital, Ibadan. The purified genomic DNA was quantified using NanoDropTM 2000 spectrophotometer. The region of interest was amplified using Polymerase Chain Reaction (PCR), this was quantified on a 1.5% agarose gel, and the ethidium bromide stained gel was viewed on a transilluminator. The PCR products were sequenced at Inqaba Biotec, and chromatogram was analyzed using FinchTv and SnapGene Viewer. Nucleotide BLAST was performed on the DNA sequence for sequence identity and retrieval of reference sequence. Multiple Sequence Alignment using T-COFFEE was performed to reveal the polymorphic variations in the samples. The outcome of this study showed nineteen identified polymorphic variants; Missense mutations occurred in 47% of the samples, 32% were silent mutations, 16% were frameshift mutation and 5% nonsense mutation. Sociodemographic characteristics revealed that 60% of the study participants has husbands with multiple sexual partners and that only 23.3% of the participants have ever done pap smear test prior to diagnosis, while 20% of them are unaware of the screening test. The histopathological result, alongside the Multiple Sequence Alignment revealed that with higher severity of cervical carcinoma, the p53 gene tends to accumulate more mutations. Our study also revealed the poor knowledge of Nigerian women to cancer screening, this may be one of the contributory factors to late diagnosis of cervical cancer among the African population. Hence, awareness for uptake of cancer screening and early detection are encouraged.

scholarly journals Apocrine sweat gland carcinoma

Eye ◽  
2001 ◽  
Vol 15 (2) ◽  
pp. 253-254 ◽  
Author(s):  
Dion Paridaens ◽  
Cornelia M Mooy
Keyword(s):  
Sweat Gland ◽  
Sweat Gland Carcinoma ◽  
Apocrine Sweat Gland ◽  
Gland Carcinoma

scholarly journals Malignant and Noninvasive Skin Tumours in Renal Transplant Recipients

2014 ◽  
Vol 2014 ◽  
pp. 1-6
Author(s):  
Christopher D. Roche ◽  
Joelle S. Dobson ◽  
Sion K. Williams ◽  
Mara Quante ◽  
Joyce Popoola ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Skin Tumours ◽  
Skin Malignancy ◽  
Increased Risk ◽  
Malignant Skin ◽  
Risk Of Malignancy

Background.Transplant recipients require immunosuppression to prevent graft rejection. This conveys an increased risk of malignancy, particularly skin tumours. There is a need for up-to-date data for the South of England.Method.Pathology records were reviewed for 709 kidney transplant recipients on immunosuppression at our hospital from 1995 to 2008. Skin tumours were recorded/analysed.Results.Mean age at transplant was 46 years. Mean length of follow-up was 7.2 years and total follow-up was 4926 person-years. 53 (7.5%) patients (39/458 (8.5%) males and 14/251 (5.6%) females) developed ≥1 skin malignancy. Cumulative incidences of 4.0%, 7.5%, and 12.2% were observed for those with <5, <10, and ≥10 years follow-up, respectively. The rate was 45 tumours per 1000 person-years at risk. Additionally, 21 patients (3.0%) only had noninvasive tumours. 221 malignant skin tumours were found: 50.2% were SCCs, 47.1% BCCs, and 2.7% malignant melanomas. Mean years to first tumour were 5.8. Mean number of tumours per patient was 4, with mean interval of 12 months.Conclusions.Despite changes in transplantation practice during the time since the last data were published in this region, these findings are similar to previous studies. This adds to the evidence allowing clinicians to inform patients in this region of their risk.

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