scholarly journals A preliminary study on the effect of mineralization parameters on determination of metals in Viscum album species

2010 ◽  
Vol 8 (2) ◽  
pp. 264-268 ◽  
Author(s):  
Eliza Blicharska ◽  
Łukasz Komsta ◽  
Ryszard Kocjan ◽  
Anna Gumieniczek ◽  
Artur Robak

AbstractAn increasing interest in determination of various macro- and microelements in medicinal plants has been observed. The majority of studies are carried out using one mineralization method without any optimization. The present study demonstrates that changes in mineralization parameters can significantly affect the recovery of the elements determined. In the study, the dried plant material was mineralized in 12 ways and iron (Fe), copper (Cu), zinc (Zn), nickel (Ni) and manganese (Mn) levels were determined. The samples were mineralized in the dry or open microwave mode as well as 10 closed microwave modes. The influence of acid amounts, irradiation power and time, addition of hydrogen peroxide and perfluoric acid was examined. All parameters were shown to be critical — good efficiency was observed with larger amounts of acid. The determined content varied significantly in the same sample and were in the ranges (ug g−1): 46–136 (Fe), 1.4–11.8 (Cu), 4.0–11.3 (Ni), 15.4–53.8 (Zn) and 9.5–67.6 (Mn). Increased irradiation resulted in the loss of copper and zinc and better recovery of nickel. The results demonstrate that such determinations should include the mineralization optimization step.

Author(s):  
A. Megalinska ◽  
M. Sokulska

The article is devoted to the important problem of the search of herbal preparations with high antibacterial, cytostatic and lithic activity. The plants such as Ambrosia artemisiifolia, which belongs to the family of Aystrou, are promising in this direction. The aim of the presented work is to study the antibacterial, cytostatic and lithic activity of water extracts of Ambrosia polynolithic and conduct a comparative analysis of these properties with other medicinal plants. Determining that the lectin extract from the green shoots (until flowering) of Ambrosia artemisiifolia does not agglutinate red blood cells, makes it possible to consider this raw material as a potential drug in pharmacology. Investigation of cytostatic activity by the method of Ivanov and Bystrova enabled to establish a place of ambrosia among already well-known medicinal plants. The cytostatic activity of the investigated plants can be represented by the following series in the order of reduction: Chelidónium május, Ambrosia artemisiifolia, Vinca minor, Viscum album. The establishment of antibacterial activity allowed to confirm the presence of anti-staphylococcal activity of the water extract of ambrosia and its significant effect on the colon. Anti-staphylococcus activity of the water extract of Ambrosia artemisiifolia is close to Viscum album and Chelidónium május. The antibacterial effect against Proteus vulgaris was the highest in the extract of аmbrosiа shoots, compared with other medicinal plants studied. Taking into account the phylogenetic method, it could be expected that the plants of the Aystroe family may have a lithic activity with respect to the concrements that are formed in the kidneys of man. So, a study was conducted on the lithic activity of polystyrene embryos and it was found that Ambrosia artemisiifolia has a high lithic activity in relation to urate and oxalate concretions.


Proceedings ◽  
2019 ◽  
Vol 15 (1) ◽  
pp. 7 ◽  
Author(s):  
Helena Vasconcelos ◽  
José M. M. M. de Almeida ◽  
Cristina Saraiva ◽  
Pedro A. S. Jorge ◽  
Luis Coelho

High concentration of biogenic amines (BA) is an indicator of deterioration of food and the determination of their concentration is an important method of food control. The hydrogen peroxide (H2O2) is a side product of the degradation of BAs by certain enzymes. It is presented an experimental technique grounded on chemiluminescence to measure small quantities of H2O2 with concentrations as low as 0.01%w/w up to 0.08%w/w. Luminol and cobalt hydroxide are added to hydroxyethyl cellulose to obtain an active membrane which will react with the sampling solution and the amount of total light emission is directly related to the H2O2 concentration.


1986 ◽  
Vol 69 (6) ◽  
pp. 1006-1008
Author(s):  
Helen D Skaltsa ◽  
Michael A Koupparis ◽  
Skevos M Philianos

Abstract A kinetic potentiometric method is described for the determination of peroxidases in vegetables and medicinal plants. It is based on the measurement of fluoride ion liberation rate from the peroxidase-catalyzed reaction of p-fluorophenol with hydrogen peroxide, sing the fluoride ion-selective electrode. The calibration curve is linear in the range 1-10 mU/mL with a precision of 1-3%. No interference was found from plant extracts, and recoveries were 97-107% (mean 100.9%). The method is rapid (2 min per measurement) and applicable to highly colored or turbid solutions. The method was applied for the determination of peroxidase activity in various plants of Greek flora. Plants of Leguminosae were found to be the richest source.


2020 ◽  
Vol 2020 ◽  
Author(s):  
Firew Admasu

Abstract: The study were conducted at Dilla University, College of Natural Sciences, Biological Sciences laboratories. Background: Ethiopia is a country with many ethnic groups, cultures and beliefs which in turn have contributed to the high diversity of traditional health care knowledge and practices of traditional medicine from local growth plants, animals and minerals for various physical and mental disorders of human and livestock population that passed from generation to generation for centuries. Medicinal plants contributors to pharmaceutical, agricultural and food industries in the world. The use of medicinal plants in the industrialized societies has been traced to extraction and development of several drugs used in order to heel some diseases having inhibiting effect against pathogenic microorganism. Objective: The main objective of this study was Extraction and Phytochemicals determination of traditional medicinal plants for anti microbial susceptibility test. Methodology: The extraction and identification of some phytochemicals crude compound which used for antimicrobial susceptibility test from plant sample such as Ocimum lamiifolium (OL), Croton maerosth (Cm) and Ruta chalepesis (RC) were conducted. Plant samples are collected, powdered using mortal and pistil and extracted using ethanol and some susceptibility tests were performed to identify some phytochemicals compound. Result: The main result of Antimicrobial activity test showed that the crude extract of OL has the highest zone of inhibition. The highest yield of crude extract (38.21%) was obtained from Croton maerosth (CM) which followed by Ruta chalepesis (RC) (32.43%). However, the lowest yield (28.37%) was obtained from Oscpmum lamifolium (OL). Conclusion: Traditional Medicine is used by many people to managing numerous conditions; it’s accessible and effective on antimicrobial activity. Therefore, it plays a significant role by reducing life-threatening ailments of people and other animals.


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