Extraction and Phytochemicals Determination of Traditional Medicinal Plants for Anti-microbial Susceptibility Test

2020 ◽  
Vol 2020 ◽  
Author(s):  
Firew Admasu
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Traditional Medicine ◽  
Crude Extract ◽  
Susceptibility Test ◽  
Plant Sample ◽  
Traditional Medicinal Plants ◽  
Traditional Health Care ◽  
Susceptibility Tests

Abstract: The study were conducted at Dilla University, College of Natural Sciences, Biological Sciences laboratories. Background: Ethiopia is a country with many ethnic groups, cultures and beliefs which in turn have contributed to the high diversity of traditional health care knowledge and practices of traditional medicine from local growth plants, animals and minerals for various physical and mental disorders of human and livestock population that passed from generation to generation for centuries. Medicinal plants contributors to pharmaceutical, agricultural and food industries in the world. The use of medicinal plants in the industrialized societies has been traced to extraction and development of several drugs used in order to heel some diseases having inhibiting effect against pathogenic microorganism. Objective: The main objective of this study was Extraction and Phytochemicals determination of traditional medicinal plants for anti microbial susceptibility test. Methodology: The extraction and identification of some phytochemicals crude compound which used for antimicrobial susceptibility test from plant sample such as Ocimum lamiifolium (OL), Croton maerosth (Cm) and Ruta chalepesis (RC) were conducted. Plant samples are collected, powdered using mortal and pistil and extracted using ethanol and some susceptibility tests were performed to identify some phytochemicals compound. Result: The main result of Antimicrobial activity test showed that the crude extract of OL has the highest zone of inhibition. The highest yield of crude extract (38.21%) was obtained from Croton maerosth (CM) which followed by Ruta chalepesis (RC) (32.43%). However, the lowest yield (28.37%) was obtained from Oscpmum lamifolium (OL). Conclusion: Traditional Medicine is used by many people to managing numerous conditions; it’s accessible and effective on antimicrobial activity. Therefore, it plays a significant role by reducing life-threatening ailments of people and other animals.

Pemanfaatan Tumbuhan Obat Tradisional oleh Etnis Sangihe di Kepulauan Sangihe Bagian Selatan, Sulawesi Utara (The Usage of Traditional Medicinal Plants by Sangihe Ethnic in the Southern Sangihe Islands, North Sulawesi)

2018 ◽  
Vol 8 (2) ◽  
pp. 45
Author(s):  
Chrisye Yustitia Pelokang ◽  
Roni Koneri ◽  
Deidy Katili
Keyword(s):  
Medicinal Plants ◽  
Traditional Medicine ◽  
Plant Species ◽  
Structured Interviews ◽  
Active Components ◽  
Plant Materials ◽  
Medical Practitioners ◽  
North Sulawesi ◽  
Traditional Medicinal Plants ◽  
Plant Families

Abstrak Tumbuhan obat merupakan tumbuhan yang menghasilkan satu atau lebih komponen aktif yang dipercaya oleh penduduk berkhasiat obat sehingga dimanfaatkan dalam pengobatan tradisional. Penelitian ini bertujuan untuk mengidentifikasi dan mengkaji spesies tumbuhan yang digunakan sebagai obat tradisional oleh Etnis Sangihe di Kepulauan Sangihe bagian Selatan, Sulawesi Utara. Pengambilan data dilakukan melalui wawancara terstruktur  yang diajukan kepada pengobat tradisional. Hasil penelitian menunjukkan adanya 38 spesies dari 25 famili tumbuhan yang dimanfaatkan sebagai tumbuhan obat oleh Etnis Sangihe bagian Selatan. Herba merupakan habitus tumbuhan yang banyak dimanfaatkan untuk bahan pengobatan. Bagian tumbuhan yang paling banyak digunakan sebagai obat yaitu daun. Cara pengolahan yang paling banyak digunakan adalah direbus. Jenis penyakit yang dapat diobati dengan tumbuhan obat sebanyak 22 jenis penyakit. Kata kunci: tumbuhan obat, obat tradisional, habitus, Kepulauan Sangihe Bagian Selatan Abstract             Medicinal plants are plants that produce one or more active components that are believed by local people as medicinal plants for traditional medicine practices. This study aimed to identify and to assess the plant species that used as traditional medicine by the Sangihe Ethnic in the Southern Sangihe Islands, North Sulawesi. Data collection was conducted by structured interviews to the indigenous medical practitioners. The results showed that 38 plant species from 25 plant families were used as medicinal plants by the Southern Sangihe Ethnic people. Herbs were plant habitus that were widely used for medicinal ingredients. The leaves were widely used as medicinal plant materials. Boiling was the most processing method for preparing medicinal herbs. There were 22 types of diseases that could be treated using medicinal plants. Keywords: medicinal plants, traditional medicine, habitus, Southern Sangihe Islands

scholarly journals Evaluation of Antimicrobial Activity of the Methanol Extracts from 8 Traditional Medicinal Plants

2011 ◽  
Vol 27 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Chang-Geun Kang ◽  
Dae-Sik Hah ◽  
Chung-Hui Kim ◽  
Young-Hwan Kim ◽  
Eui-Kyung Kim ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Methanol Extracts ◽  
Traditional Medicinal Plants

A new ent-kaurane from the root extract of Chiococcaalba

1997 ◽  
Vol 75 (6) ◽  
pp. 801-804 ◽  
Author(s):  
Rocío Borges-Argáez ◽  
Leticia Medina-Baizabál ◽  
Filogonio May-Pat ◽  
Luis M. Peña-Rodríguez
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Crude Extract ◽  
Chemical Shifts ◽  
Root Extract ◽  
Spectroscopic Methods ◽  
Chemical Transformations ◽  
Bioactive Metabolite ◽  
H Nmr ◽  
C Nmr

A bioassay-guided purification of the methanolic crude extract obtained from the roots of Chiococcaalba (L.) Hitchc. resulted in the isolation of a new, bioactive, metabolite identified as ent-17-hydroxy-16α-kauran-3-one (1). Elucidation of the new structure was based on analyses of the results obtained from various spectroscopic methods (IR, MS, 1H NMR, and 13C NMR) and chemical transformations. The stereochemistry at C16 was assigned by comparing both the proton and carbon chemical shifts of C17 with those reported in the literature. The new kaurane showed weak antimicrobial activity when tested against Staphylococcusaureus. Keywords: medicinal plants, Chiococcaalba, Rubiaceae, kaurane, diterpene, antimicrobial.

scholarly journals Antimicrobial Activity of Selected Medicinal Plants from a Sub-Saharan African Country Against Bacterial Pathogens from Post-Operative Wound Infections

2021 ◽  
Vol 9 (2) ◽  
pp. 23
Author(s):  
Enid Owusu ◽  
Martin Mensah Ahorlu ◽  
Emmanuel Afutu ◽  
Amos Akumwena ◽  
George Awuku Asare
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Traditional Medicine ◽  
Chronic Wounds ◽  
Bacterial Pathogens ◽  
Antimicrobial Activities ◽  
Aureus Strain ◽  
Diffusion Method ◽  
Phytochemical Analysis

Background: Globally, the application of medicinal plants in the management of acute and chronic wounds can be considered a common occurrence in most traditional medicine practices. In view of this, many plants in the tropical and subtropical regions have been screened for their wound-healing activities. Consequently, plants having antimicrobial activity against multidrug-resistant (MD-R) pathogens can be considered great assets. Therefore, this study evaluated ethanolic and aqueous extracts of five medicinal plants (Psidium guajava, Myrianthus arboreus, Alchornea cordifolia, Momordica charantia, and Justicia flava) for their antimicrobial activities against MD-R bacterial pathogens isolated from post-operative wounds; Methods: This involved the aqueous and ethanolic extraction of the selected medicinal plants. Preliminary phytochemical constituents of the plants were examined. The agar well diffusion method was then used to determine the antibacterial activity of the leaves against reference strains (Escherichia coli ATCC 25922, Salmonella typhi ATCC 19430, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923, and a Methicillin-Resistant Staphylococcus aureus strain) as well as the MDR clinical isolates (E. coli, P. aeruginosa, S. aureus and CoNS) from the wounds; Results: The preliminary phytochemical analysis of the leaves showed the presence of saponins, phenolics, and reducing sugars in almost all the plants tested. All plant extracts were observed to have some antimicrobial activity against at least one reference strain. For the clinical MDR isolates, A. cordifolia from this study showed highest inhibition to growth of all bacteria used. Activity of J. flava against S. aureus was highest as compared to that of E.coli and P. aeruginosa. Similar observation was made for M. arboreus, P. guajava and M. charantia where the highest activity was observed against S. aureus; Conclusion: This study has mainly shown that P. guajava, M. arboreus, A. cordifolia, M. charantia, and J. flava exhibits antimicrobial activities against MD-R bacterial pathogens isolated from post-operative wounds. Also, these plants has bioactive phytochemical compounds with potential medicinal values for the treatment of numerous infections. Therefore, these plants may be helpful in the management of acute and chronic wounds, especially in traditional medicine practices.

scholarly journals Antimicrobial Study of Chloroform Fraction from the Leaves of Entada spiralis Ridl.

2021 ◽  
Vol 1 (1) ◽  
pp. 45-53
Author(s):  
Fitri Rachmadita ◽  
Erryana Martati ◽  
Sharifah Nurul Akilah Syed Mohamad ◽  
Siti Zaiton Mat So'ad
Keyword(s):  
Antimicrobial Activity ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Inhibition Zone ◽  
Food Preservation ◽  
Total Phenolic ◽  
Chloroform Fraction ◽  
Disk Diffusion Test ◽  
E Coli

Introduction: Entada Spiralis Ridl., or locally identified as Sintok, contains flavonoid, saponin, tannin, and glycoside, compounds that have antifungal and antibacterial activities. This research aims to identify bioactive compounds and determine the antimicrobial activity from crude and fraction of E. spiralis extract. Methods: The crude extract was prepared by macerating the leaves with chloroform, and then proceeded to fraction it by vacuum liquid chromatography with Dichloromethane (DCM)/Hexane (Hex) (1/9) and Dichloromethane (DCM)/Methanol (MeOH) (9/1) solvent system. Disk Diffusion Test and Microdilution Assay evaluated the extracts' antimicrobial activity against S. aureus, E. coli and C. albicans. The determination of bioactive compounds was done by Thin Layer Chromatography (TLC). Determination of Total Phenolic (TPC) and Flavonoid Content (TFC) were performed by Folin-Ciocalteu and AlCl3 Colourimetric Assay Results: The greatest inhibition zone against C. albicans was obtained from fraction Chloroform (CHCl3) extract with an inhibition zone of 10.33 mm. DCM/MeOH (9/1) effectively killed S. aureus and E.coli with an inhibition zone of 11.67 and 12 mm, respectively. The minimum inhibitory concentration (MIC) of CHCl3 crude extract were 1.563 mg/mL for both E. coli and S. aureus, and 0.781 mg/mL for C. albicans. The TLC revealed the presence of tannins, saponin, glycosides, phenol, flavonoid, triterpenoid, and aromatic compound in CHCl3 crude extract. TPC of DCM/MeOH (9/1), CHCl3, and DCM/Hex (1/9) were 50.56 ± 0.188, 51.913± 0.089, 24.16 ± 0.175 mg GAE/g extract. Conclusion: In conclusion, E. spiralis leaves could be a source of active antifungal and antimicrobial agents used for food preservation by using a semipolar solvent for extraction.

scholarly journals PEMANFAATAN TUMBUHAN OBAT OLEH MASYARAKAT DESA GUNUNG SEMBILAN KECAMATAN SUKADANA KABUPATEN KAYONG UTARA

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Muhamad Sauji ◽  
H A Oramahi ◽  
M Idham
Keyword(s):  
Medicinal Plants ◽  
Raw Materials ◽  
Sampling Technique ◽  
Processing Method ◽  
Snowball Sampling ◽  
Village Community ◽  
Direct Observations ◽  
Traditional Medicinal Plants ◽  
Snowball Sampling Technique

Medicinal plants are one of the important components in medicine, in the form of traditional herbal concoctions and have been used since hundreds of years ago, to solve various health problems. This study aims to obtain the spesies of traditional medicinal plants used by the community in Gunung Sembilan Village in treating diseases and to know the use of medicinal plants and how to concoct medicinal raw materials. Data collection with interview and determination of responden uses a snowball sampling technique or carried out sequentially by asking for information on people who have been interviewed or contacted before the research. Observations were made, namely by walking while (without plots) making direct observations in the field together with respondents who knew about the medicinal plants. The results of interviews with the community of Gunung Sembilan Village, Sukadana District North Kayong Regency, 27 species of medicinal plants and 23 families were utilized by the community. The use of herbs is single or mixed, the most widely used are leaves (10 species or 37.04%). The most commonly used processing method is pounded (12 species or 44.44%), and how to consume the most frequently done, which is drink (12 species or 44.44%).Keywords : Gunung Sembilan village community, medicinal plants.

Sign in / Sign up

Export Citation Format

Share Document